LOCUS       62056_18900        4916 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             1023..1250
                     /codon_start=1
                     /label=ubiquitin-K48
                     /note="human ubiquitin variant containing Lys-48, with all
                     other lysines mutated to arginine"
                     /translation="MQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQRESTLHLVLRLRGG"
     polyA_signal    1300..1434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1570..1780
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     primer_bind     complement(1808..1824)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2037..2492
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2774..2878
                     /label=AmpR promoter
     CDS             2879..3736
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3910..4499
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4787..4808
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4823..4853
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4861..4877
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4885..4901
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcccgaac cgacagtcgg tctcttcacc aaggccattc
      961 gcgccaccat gggccaccat caccatcacc aagatctcaa tggtggtggt ggtgggtcga
     1021 ccatgcagat cttcgtcaga acgttaaccg gtagaaccat aactctagaa gttgaaccat
     1081 ccgataccat cgaaaacgtt agagctagaa ttcaagacag agaaggcatt ccacctgatc
     1141 aacaaagatt gatctttgcc ggtaagcagc tcgaggacgg tagaacgctg tctgattaca
     1201 acattcagag agagtccacc ctgcacctgg tcctccgtct cagaggtggt tgagcggccg
     1261 ctaagtaagt aaggatcccc agcttggccg ccatggccca acttgtttat tgcagcttat
     1321 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     1381 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcgggaat
     1441 taattcggcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg gttaggtacc
     1501 ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     1561 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     1621 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     1681 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     1741 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctgttaaca
     1801 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     1861 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     1921 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt
     1981 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
     2041 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     2101 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     2161 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     2221 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
     2281 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     2341 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattctttta atttataagg
     2401 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     2461 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
     2521 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     2581 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     2641 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     2701 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2761 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2821 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2881 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2941 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     3001 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     3061 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3121 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3181 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3241 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3301 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3361 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3421 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3481 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3541 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3601 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3661 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3721 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3781 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3841 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3901 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3961 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4021 aactggcttc agcagagcgc agataaccaa atactgttct tctagtgtag ccgtagttag
     4081 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4141 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4201 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4261 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4321 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     4381 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     4441 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     4501 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     4561 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     4621 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     4681 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     4741 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     4801 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     4861 ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg aattaa
//
