LOCUS       62056_18895        4718 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4718
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    1102..1236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1372..1582
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     primer_bind     complement(1610..1626)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1839..2294
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2576..2680
                     /label=AmpR promoter
     CDS             2681..3538
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3712..4301
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4589..4610
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4625..4655
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4663..4679
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4687..4703
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcccgaac cgacagtcgg tctcttcacc aaggccattc
      961 gcgccaccat gggccaccat caccatcacc aagatctcaa tggtggtggt ggtgggtcga
     1021 cgtacccggg cgtcaagctt gctagatatc cgcatgcggc cgctaagtaa gtaaggatcc
     1081 ccagcttggc cgccatggcc caacttgttt attgcagctt ataatggtta caaataaagc
     1141 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1201 tccaaactca tcaatgtatc ttatcatgtc tggatcggga attaattcgg cgcagcacca
     1261 tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac
     1321 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     1381 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     1441 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     1501 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     1561 ggcttttttg gaggcctagg cttttgcaaa aagctgttaa cagcttggca ctggccgtcg
     1621 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     1681 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     1741 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
     1801 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
     1861 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     1921 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     1981 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     2041 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     2101 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     2161 aacactcaac cctatctcgg gctattcttt taatttataa gggattttgc cgatttcggc
     2221 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     2281 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2341 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     2401 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     2461 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     2521 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2581 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2641 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2701 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2761 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2821 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     2881 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     2941 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     3001 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     3061 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3121 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3181 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3241 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3301 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3361 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3421 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3481 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3541 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3601 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3661 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3721 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3781 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3841 gcagataacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3901 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3961 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     4021 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     4081 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     4141 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     4201 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     4261 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     4321 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     4381 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     4441 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     4501 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     4561 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     4621 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     4681 ttcacacagg aaacagctat gacatgatta cgaattaa
//
