LOCUS       40924_37143        9816 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRK442, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Scott HN, Laible PD, Hanson DK.
  TITLE     Sequences of versatile broad-host-range vectors of the RK2 family
  JOURNAL   Plasmid 50 (1), 74-79 (2003)
  PUBMED    12826060
REFERENCE   2  (bases 1 to 9816)
  AUTHORS   Scott HN, Laible PD, Hanson DK.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2002) Biosciences Division, Argonne National 
            Laboratory, 9700 South Cass Ave., Argonne, IL 60439, USA
REFERENCE   3  (bases 1 to 9816)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9816)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2003"; volume: "50"; issue: "1"; pages: "74-79"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2002) Biosciences Division, Argonne National Laboratory, 
            9700 South Cass Ave., Argonne, IL 60439, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(152..863)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     primer_bind     1373..1389
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1438..1454)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1462..1478)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1486..1516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1531..1552)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1787..2155)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(2188..2297)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2981..3628)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             3734..4930
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(6209..7354)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8081..9175)
                     /codon_start=1
                     /gene="incC"
                     /product="plasmid maintenance protein"
                     /label=incC
                     /protein_id="AAO47421.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            complement(8081..9175)
                     /gene="incC"
                     /label=incC
     CDS             complement(8857..9162)
                     /codon_start=1
                     /gene="korA"
                     /product="regulation of gene expression"
                     /label=korA
                     /protein_id="AAO47422.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            complement(8857..9162)
                     /gene="korA"
                     /label=korA
ORIGIN
        1 ccacccaggc cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc
       61 tgcggcacgt caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc
      121 ccgatcccgg caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg
      181 gccgaggggc gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg
      241 agaagggggg gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt
      301 aaaaacaagg tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc
      361 gaaaaacggg cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa
      421 tgtcaatagg tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc
      481 ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag
      541 gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga
      601 ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc
      661 gccgggtgag tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa
      721 gttttccgcg aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg
      781 cgtttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag
      841 cccggtgagc gtcggaaagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      901 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      961 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1021 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1201 tgtccgcctt tctcccttcg ggaagcgtgg cgccattcgc cattcaggct gcgcaactgt
     1261 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     1321 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     1381 acggccagtg aattcccggg gatccgtcga cctgcagcca agcttggcgt aatcatggtc
     1441 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     1501 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     1561 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     1621 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctcg gtcttgcctt gctcgtcggt
     1681 gatgtacttc accagctccg cgaagtcgct cttcttgatg gagcgcatgg ggacgtgctt
     1741 ggcaatcacg cgcacccccc ggccgtttta gcggctaaaa aagtcatggc tctgccctcg
     1801 ggcggaccac gcccatcatg accttgccaa gctcgtcctg cttctcttcg atcttcgcca
     1861 gcagggcgag gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg gtgagccaga
     1921 gtttcagcag gccgcccagg cggcccaggt cgccattgat gcgggccagc tcgcggacgt
     1981 gctcatagtc cacgacgccc gtgattttgt agccctggcc gacggccagc aggtaggccg
     2041 acaggctcat gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc
     2101 agtacacctt gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct
     2161 tgccctcatc tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga
     2221 gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta
     2281 cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc
     2341 tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa
     2401 tgaccccgaa gcagggttat gcagcggaaa agcgccacgc ttcccgaagg gagaaaggcg
     2461 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2521 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2581 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2641 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2701 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     2761 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgccagaa ggccgccaga
     2821 gaggccgagc gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa gcccgtagcg
     2881 ggctgctacg ggcgtctgac gcggtggaaa gggggagggg atgttgtcta catggctctg
     2941 ctgtagtgag tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc tttctcggtc
     3001 cttcaacgtt cctgacaacg agcctccttt tcgccaatcc atcgacaatc accgcgagtc
     3061 cctgctcgaa cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg gcccgcaaca
     3121 gcggcgagag cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg ctgtcgccgg
     3181 cctgctcctc aagcacggcc ccaacagtga agtagctgat tgtcatcagc gcattgacgg
     3241 cgtccccggc cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca
     3301 tctgcggtgc gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca
     3361 gcgcctgcct gaagctgcgg gcattcccga tcagaaatga gcgccagtcg tcgtcggctc
     3421 tcggcaccga atgcgtatga ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg
     3481 cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg aacccccaac cgttccgcca
     3541 gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa caggtccagg gcggcacgga
     3601 tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata aacataatat
     3661 gtccaccaac ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt
     3721 ccggaggcca gacatgaaac ccaacatacc cctgatcgta attctgagca ctgtcgcgct
     3781 cgacgctgtc ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt
     3841 tcactcgaac gacgtcaccg cccactatgg cattctgctg gcgctgtatg cgttggtgca
     3901 atttgcctgc gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc ggccaatctt
     3961 gctcgtctcg ctggccggcg ccactgtcga ctacgccatc atggcgacag cgcctttcct
     4021 ttgggttctc tatatcgggc ggatcgtggc cggcatcacc ggggcgactg gggcggtagc
     4081 cggcgcttat attgccgata tcactgatgg cgatgagcgc gcgcggcact tcggcttcat
     4141 gagcgcctgt ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg
     4201 tttctccccc cacgctccgt tcttcgccgc ggcagccttg aacggcctca atttcctgac
     4261 gggctgtttc cttttgccgg agtcgcacaa aggcgaacgc cggccgttac gccgggaggc
     4321 tctcaacccg ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg ccgccctgat
     4381 ggcggtcttc ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt
     4441 cggcgaggat cgctttcact gggacgcgac cacgatcggc atttcgcttg ccgcatttgg
     4501 cattctgcat tcactcgccc aggcaatgat caccggccct gtagccgccc ggctcggcga
     4561 aaggcgggca ctcatgctcg gaatgattgc cgacggcaca ggctacatcc tgcttgcctt
     4621 cgcgacacgg ggatggatgg cgttcccgat catggtcctg cttgcttcgg gtggcatcgg
     4681 aatgccggcg ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc aggggcagct
     4741 gcaaggctca ctggcggcgc tcaccagcct gacctcgatc gtcggacccc tcctcttcac
     4801 ggcgatctat gcggcttcta taacaacgtg gaacgggtgg gcatggattg caggcgctgc
     4861 cctctacttg ctctgcctgc cggcgctgcg tcgcgggctt tggagcggcg cagggcaacg
     4921 agccgatcgc tgatcgtgga aacgataggc ctatgccatg cgggtcaagg cgacttccgg
     4981 caagctatac gcgccctagg agtgcggttg gaacgttggc ccagccagat actcccgatc
     5041 acgagcagga cgccgatgat ttgaagcgca ctcagcgtct gatccaagaa caaccatcct
     5101 agcaacacgg cggtccccgg gctgagaaag cccagtaagg aaacaactgt aggttcgagt
     5161 cgcgagatcc cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac
     5221 gccaggccga gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct
     5281 actggaacga gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc
     5341 acgggaggtt gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc
     5401 aggcccgctg cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc
     5461 acgcccgcag ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc
     5521 agagcggcag agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc
     5581 ccgcccggca ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt
     5641 gcgccgagga tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc
     5701 acgagcaata aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc
     5761 tgttcgggct ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc
     5821 tcctgtttga agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg
     5881 gcatctcgca accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg
     5941 gacccgaaca tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc
     6001 acgtcggccg ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct
     6061 ctgtttatcg gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag
     6121 tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc
     6181 cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc
     6241 aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc
     6301 gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga
     6361 gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca
     6421 gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga
     6481 tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga
     6541 agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg
     6601 ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg
     6661 accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg
     6721 tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct
     6781 cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca
     6841 gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt
     6901 ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt
     6961 ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt
     7021 ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga
     7081 tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg
     7141 cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg
     7201 ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga
     7261 cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt
     7321 gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga
     7381 ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca
     7441 gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt
     7501 acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc
     7561 cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct
     7621 tgtcgccggt cgtggccgcg ccaacctttg cgatccgcaa gcgcgcggtc gccatcttca
     7681 cgctggaaca gtacgtcgag gcgggcatca tgacccgcga gcaatacgag gtcattaaaa
     7741 gcgccgtgat tgatgatata gcggcccggc tgctcctggt tctcgcgcac cgaaatgggt
     7801 gacttcaccc cgcgctcttt gatcgtggca ccgatttccg cgatgctctc cggggaaaag
     7861 ccggggttgt cggccgtccg cggctgatgc ggatcttcgt cgatcaggtc caggtccagc
     7921 tcgatagggc cggaaccgcc ctgagacgcc gcaggagcgt ccaggaggct cgacaggtcg
     7981 ccgatgctat ccaaccccag gccggacggc tgcgccgcgc ctgcggcttc ctgagcggcc
     8041 gcagcggtgt ttttcttggt ggtcttggct tgagccgcag tcattgggaa atctccatct
     8101 tcgtgaacac gtaatcagcc agggcgcgaa cctctttcga tgccttgcgc gcggccgttt
     8161 tcttgatctt ccagaccggc acaccggatg cgagggcatc ggcgatgctg ctgcgcaggc
     8221 caacggtggc cggaatcatc atcttggggt acgcggccag cagctcggct tggtggcgcg
     8281 cgtggcgcgg attccgcgca tcgaccttgc tgggcaccat gccaaggaat tgcagcttgg
     8341 cgttcttctg gcgcacgttc gcaatggtcg tgaccatctt cttgatgccc tggatgctgt
     8401 acgcctcaag ctcgatgggg gacagcacat agtcggccgc gaagagggcg gccgccaggc
     8461 cgacgccaag ggtcggggcc gtgtcgatca ggcacacgtc gaagccttgg ttcgccaggg
     8521 ccttgatgtt cgccccgaac agctcgcggg cgtcgtccag cgacagccgt tcggcgttcg
     8581 ccagtaccgg gttggactcg atgagggcga ggcgcgcggc ctggccgtcg ccggctgcgg
     8641 gtgcggtttc ggtccagccg ccggcaggga cagcgccgaa cagcttgctt gcatgcaggc
     8701 cggtagcaaa gtccttgagc gtgtaggacg cattgccctg ggggtccagg tcgatcacgg
     8761 caacccgcaa gccgcgctcg aaaaagtcga aggcaagatg cacaagggtc gaagtcttgc
     8821 cgacgccgcc tttctggttg gccgtgacca aagttttcat cgtttggttt cctgtttttt
     8881 cttggcgtcc gcttcccact tccggacgat gtacgcctga tgttccggca gaaccgccgt
     8941 tacccgcgcg tacccctcgg gcaagttctt gtcctcgaac gcggcccaca cgcgatgcac
     9001 cgcttgcgac actgcgcccc tggtcagtcc cagcgacgtt gcgaacgtcg cctgtggctt
     9061 cccatcgact aagacgcccc gcgctatctc gatggtctgc tgccccactt ccagcccctg
     9121 gatcgcctcc tggaactggc tttcggtaag ccgtttcttc atggataaca cccataattt
     9181 gctccgcgcc ttggttgaac atagcggtga cagccgccag cacatgagag aagtttagct
     9241 aaacatttct cgcacgtcaa cacctttagc cgctaaaact cgtccttggc gtaacaaaac
     9301 aaaagcccgg aaaccgggct ttcgtctctt gccgcttatg gctctgcacc cggctccatc
     9361 accaacaggt cgcgcacgcg cttcactcgg ttgcggatcg acactgccag cccaacaaag
     9421 ccggttgccg ccgccgccag gatcgcgccg atgatgccgg ccacaccggc catcgcccac
     9481 caggtcgccg ccttccggtt ccattcctgc tggtactgct tcgcaatgct ggacctcggc
     9541 tcaccatagg ctgaccgctc gatggcgtat gccgcttctc cccttggcgt aaaacccagc
     9601 gccgcaggcg gcattgccat gctgcccgcc gctttcccga ccacgacgcg cgcaccaggc
     9661 ttgcggtcca gaccttcggc cacggcgagc tgcgcaagga cataatcagc cgccgacttg
     9721 gctccacgcg cctcgatcag ctcttgcact cgcgcgaaat ccttggcctc cacggccgcc
     9781 atgaatcgcg cacgcggcga aggctccgca gggccg
//
