LOCUS       40924_37138        9820 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRK442(H), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Scott HN, Laible PD, Hanson DK.
  TITLE     Sequences of versatile broad-host-range vectors of the RK2 family
  JOURNAL   Plasmid 50 (1), 74-79 (2003)
  PUBMED    12826060
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Scott HN, Laible PD, Hanson DK.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2002) Biosciences Division, Argonne National 
            Laboratory, 9700 South Cass Ave., Argonne, IL 60439, USA
REFERENCE   3  (bases 1 to 9820)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9820)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2003"; volume: "50"; issue: "1"; pages: "74-79"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2002) Biosciences Division, Argonne National Laboratory, 
            9700 South Cass Ave., Argonne, IL 60439, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9820
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(152..863)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     primer_bind     1373..1389
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1442..1458)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1466..1482)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1490..1520)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1535..1556)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1791..2159)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(2192..2301)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2985..3632)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             3738..4934
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(6213..7358)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8085..9179)
                     /codon_start=1
                     /gene="incC"
                     /product="plasmid maintenance protein"
                     /label=incC
                     /protein_id="AAO47427.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            complement(8085..9179)
                     /gene="incC"
                     /label=incC
     CDS             complement(8861..9166)
                     /codon_start=1
                     /gene="korA"
                     /product="regulation of gene expression"
                     /label=korA
                     /protein_id="AAO47428.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            complement(8861..9166)
                     /gene="korA"
                     /label=korA
ORIGIN
        1 ccacccaggc cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc
       61 tgcggcacgt caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc
      121 ccgatcccgg caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg
      181 gccgaggggc gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg
      241 agaagggggg gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt
      301 aaaaacaagg tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc
      361 gaaaaacggg cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa
      421 tgtcaatagg tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc
      481 ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag
      541 gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga
      601 ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc
      661 gccgggtgag tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa
      721 gttttccgcg aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg
      781 cgtttctggc gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag
      841 cccggtgagc gtcggaaagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      901 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      961 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1021 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1201 tgtccgcctt tctcccttcg ggaagcgtgg cgccattcgc cattcaggct gcgcaactgt
     1261 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     1321 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     1381 acggccagtg aattcccggg gatccgtcga cctgcagcca agctagcttg gcgtaatcat
     1441 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1501 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1561 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1621 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcggtcttg ccttgctcgt
     1681 cggtgatgta cttcaccagc tccgcgaagt cgctcttctt gatggagcgc atggggacgt
     1741 gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca tggctctgcc
     1801 ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc ttcgatcttc
     1861 gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc gtcggtgagc
     1921 cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc cagctcgcgg
     1981 acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc cagcaggtag
     2041 gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg ttcgtctgga
     2101 aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc atcagccatc
     2161 cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg
     2221 ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg
     2281 cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga
     2341 accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct
     2401 ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgcc acgcttcccg aagggagaaa
     2461 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2521 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2581 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2641 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2701 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2761 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc agaaggccgc
     2821 cagagaggcc gagcgcggcc gtgaggcttg gacgctaggg cagggcatga aaaagcccgt
     2881 agcgggctgc tacgggcgtc tgacgcggtg gaaaggggga ggggatgttg tctacatggc
     2941 tctgctgtag tgagtgggtt gcgctccggc agcggtcctg atcaatcgtc accctttctc
     3001 ggtccttcaa cgttcctgac aacgagcctc cttttcgcca atccatcgac aatcaccgcg
     3061 agtccctgct cgaacgctgc gtccggaccg gcttcgtcga aggcgtctat cgcggcccgc
     3121 aacagcggcg agagcggagc ctgttcaacg gtgccgccgc gctcgccggc atcgctgtcg
     3181 ccggcctgct cctcaagcac ggccccaaca gtgaagtagc tgattgtcat cagcgcattg
     3241 acggcgtccc cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc gtcggccgtt
     3301 tccatctgcg gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc gcggtaggcg
     3361 agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg
     3421 gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag tgcgtcgagc
     3481 agcgcccgct tgttcctgaa gtgccagtaa agcgccggct gctgaacccc caaccgttcc
     3541 gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc cagggcggca
     3601 cggatcactg tattcggctg caactttgtc atgcttgaca ctttatcact gataaacata
     3661 atatgtccac caacttatca gtgataaaga atccgcgcgt tcaatcggac cagcggaggc
     3721 tggtccggag gccagacatg aaacccaaca tacccctgat cgtaattctg agcactgtcg
     3781 cgctcgacgc tgtcggcatc ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc
     3841 tggttcactc gaacgacgtc accgcccact atggcattct gctggcgctg tatgcgttgg
     3901 tgcaatttgc ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg cggcggccaa
     3961 tcttgctcgt ctcgctggcc ggcgccactg tcgactacgc catcatggcg acagcgcctt
     4021 tcctttgggt tctctatatc gggcggatcg tggccggcat caccggggcg actggggcgg
     4081 tagccggcgc ttatattgcc gatatcactg atggcgatga gcgcgcgcgg cacttcggct
     4141 tcatgagcgc ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg
     4201 gcggtttctc cccccacgct ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc
     4261 tgacgggctg tttccttttg ccggagtcgc acaaaggcga acgccggccg ttacgccggg
     4321 aggctctcaa cccgctcgct tcgttccggt gggcccgggg catgaccgtc gtcgccgccc
     4381 tgatggcggt cttcttcatc atgcaacttg tcggacaggt gccggccgcg ctttgggtca
     4441 ttttcggcga ggatcgcttt cactgggacg cgaccacgat cggcatttcg cttgccgcat
     4501 ttggcattct gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg
     4561 gcgaaaggcg ggcactcatg ctcggaatga ttgccgacgg cacaggctac atcctgcttg
     4621 ccttcgcgac acggggatgg atggcgttcc cgatcatggt cctgcttgct tcgggtggca
     4681 tcggaatgcc ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc
     4741 agctgcaagg ctcactggcg gcgctcacca gcctgacctc gatcgtcgga cccctcctct
     4801 tcacggcgat ctatgcggct tctataacaa cgtggaacgg gtgggcatgg attgcaggcg
     4861 ctgccctcta cttgctctgc ctgccggcgc tgcgtcgcgg gctttggagc ggcgcagggc
     4921 aacgagccga tcgctgatcg tggaaacgat aggcctatgc catgcgggtc aaggcgactt
     4981 ccggcaagct atacgcgccc taggagtgcg gttggaacgt tggcccagcc agatactccc
     5041 gatcacgagc aggacgccga tgatttgaag cgcactcagc gtctgatcca agaacaacca
     5101 tcctagcaac acggcggtcc ccgggctgag aaagcccagt aaggaaacaa ctgtaggttc
     5161 gagtcgcgag atcccccgga accaaaggaa gtaggttaaa cccgctccga tcaggccgag
     5221 ccacgccagg ccgagaacat tggttcctgt aggcatcggg attggcggat caaacactaa
     5281 agctactgga acgagcagaa gtcctccggc cgccagttgc caggcggtaa aggtgagcag
     5341 aggcacggga ggttgccact tgcgggtcag cacggttccg aacgccatgg aaaccgcccc
     5401 cgccaggccc gctgcgacgc cgacaggatc tagcgctgcg tttggtgtca acaccaacag
     5461 cgccacgccc gcagttccgc aaatagcccc caggaccgcc atcaatcgta tcgggctacc
     5521 tagcagagcg gcagagatga acacgaccat cagcggctgc acagcgccta ccgtcgccgc
     5581 gaccccgccc ggcaggcggt agaccgaaat aaacaacaag ctccagaata gcgaaatatt
     5641 aagtgcgccg aggatgaaga tgcgcatcca ccagattccc gttggaatct gtcggacgat
     5701 catcacgagc aataaacccg ccggcaacgc ccgcagcagc ataccggcga cccctcggcc
     5761 tcgctgttcg ggctccacga aaacgccgga cagatgcgcc ttgtgagcgt ccttggggcc
     5821 gtcctcctgt ttgaagaccg acagcccaat gatctcgccg tcgatgtagg cgccgaatgc
     5881 cacggcatct cgcaaccgtt cagcgaacgc ctccatgggc tttttctcct cgtgctcgta
     5941 aacggacccg aacatctctg gagctttctt cagggccgac aatcggatct cgcggaaatc
     6001 ctgcacgtcg gccgctccaa gccgtcgaat ctgagcctta atcacaattg tcaattttaa
     6061 tcctctgttt atcggcagtt cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag
     6121 caagtgcgtc gagcagtgcc cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa
     6181 cccccagccg gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg
     6241 acccaggcgt gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc
     6301 tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc
     6361 ttgagcgggt acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc
     6421 gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg
     6481 acgatttcct cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg
     6541 cggaagcggt gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc
     6601 atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg
     6661 atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata
     6721 ggggtgcgct tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc
     6781 agctcgacgc cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt
     6841 tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg
     6901 tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc
     6961 atttccttga tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc
     7021 tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg
     7081 tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc
     7141 acggcggccg atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc
     7201 ttggccgtag cttgctggac catcgagccg acggactgga aggtttcgcg gggcgcacgc
     7261 atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt
     7321 tcttgcctgt atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc
     7381 ccgactcacg ccggggcaat gtgcccttat tcctgatttg acccgcctgg tgccttggtg
     7441 tccagataat ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc
     7501 tcgtacttgg tattccgaat cttgccctgc acgaatacca gcgacccctt gcccaaatac
     7561 ttgccgtggg cctcggcctg agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc
     7621 tgcttgtcgc cggtcgtggc cgcgccaacc tttgcgatcc gcaagcgcgc ggtcgccatc
     7681 ttcacgctgg aacagtacgt cgaggcgggc atcatgaccc gcgagcaata cgaggtcatt
     7741 aaaagcgccg tgattgatga tatagcggcc cggctgctcc tggttctcgc gcaccgaaat
     7801 gggtgacttc accccgcgct ctttgatcgt ggcaccgatt tccgcgatgc tctccgggga
     7861 aaagccgggg ttgtcggccg tccgcggctg atgcggatct tcgtcgatca ggtccaggtc
     7921 cagctcgata gggccggaac cgccctgaga cgccgcagga gcgtccagga ggctcgacag
     7981 gtcgccgatg ctatccaacc ccaggccgga cggctgcgcc gcgcctgcgg cttcctgagc
     8041 ggccgcagcg gtgtttttct tggtggtctt ggcttgagcc gcagtcattg ggaaatctcc
     8101 atcttcgtga acacgtaatc agccagggcg cgaacctctt tcgatgcctt gcgcgcggcc
     8161 gttttcttga tcttccagac cggcacaccg gatgcgaggg catcggcgat gctgctgcgc
     8221 aggccaacgg tggccggaat catcatcttg gggtacgcgg ccagcagctc ggcttggtgg
     8281 cgcgcgtggc gcggattccg cgcatcgacc ttgctgggca ccatgccaag gaattgcagc
     8341 ttggcgttct tctggcgcac gttcgcaatg gtcgtgacca tcttcttgat gccctggatg
     8401 ctgtacgcct caagctcgat gggggacagc acatagtcgg ccgcgaagag ggcggccgcc
     8461 aggccgacgc caagggtcgg ggccgtgtcg atcaggcaca cgtcgaagcc ttggttcgcc
     8521 agggccttga tgttcgcccc gaacagctcg cgggcgtcgt ccagcgacag ccgttcggcg
     8581 ttcgccagta ccgggttgga ctcgatgagg gcgaggcgcg cggcctggcc gtcgccggct
     8641 gcgggtgcgg tttcggtcca gccgccggca gggacagcgc cgaacagctt gcttgcatgc
     8701 aggccggtag caaagtcctt gagcgtgtag gacgcattgc cctgggggtc caggtcgatc
     8761 acggcaaccc gcaagccgcg ctcgaaaaag tcgaaggcaa gatgcacaag ggtcgaagtc
     8821 ttgccgacgc cgcctttctg gttggccgtg accaaagttt tcatcgtttg gtttcctgtt
     8881 ttttcttggc gtccgcttcc cacttccgga cgatgtacgc ctgatgttcc ggcagaaccg
     8941 ccgttacccg cgcgtacccc tcgggcaagt tcttgtcctc gaacgcggcc cacacgcgat
     9001 gcaccgcttg cgacactgcg cccctggtca gtcccagcga cgttgcgaac gtcgcctgtg
     9061 gcttcccatc gactaagacg ccccgcgcta tctcgatggt ctgctgcccc acttccagcc
     9121 cctggatcgc ctcctggaac tggctttcgg taagccgttt cttcatggat aacacccata
     9181 atttgctccg cgccttggtt gaacatagcg gtgacagccg ccagcacatg agagaagttt
     9241 agctaaacat ttctcgcacg tcaacacctt tagccgctaa aactcgtcct tggcgtaaca
     9301 aaacaaaagc ccggaaaccg ggctttcgtc tcttgccgct tatggctctg cacccggctc
     9361 catcaccaac aggtcgcgca cgcgcttcac tcggttgcgg atcgacactg ccagcccaac
     9421 aaagccggtt gccgccgccg ccaggatcgc gccgatgatg ccggccacac cggccatcgc
     9481 ccaccaggtc gccgccttcc ggttccattc ctgctggtac tgcttcgcaa tgctggacct
     9541 cggctcacca taggctgacc gctcgatggc gtatgccgct tctccccttg gcgtaaaacc
     9601 cagcgccgca ggcggcattg ccatgctgcc cgccgctttc ccgaccacga cgcgcgcacc
     9661 aggcttgcgg tccagacctt cggccacggc gagctgcgca aggacataat cagccgccga
     9721 cttggctcca cgcgcctcga tcagctcttg cactcgcgcg aaatccttgg cctccacggc
     9781 cgccatgaat cgcgcacgcg gcgaaggctc cgcagggccg
//
