LOCUS       40924_37133       10041 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRK437, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Scott HN, Laible PD, Hanson DK.
  TITLE     Sequences of versatile broad-host-range vectors of the RK2 family
  JOURNAL   Plasmid 50 (1), 74-79 (2003)
  PUBMED    12826060
REFERENCE   2  (bases 1 to 10041)
  AUTHORS   Scott HN, Laible PD, Hanson DK.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2002) Biosciences Division, Argonne National 
            Laboratory, 9700 South Cass Ave., Argonne, IL 60439, USA
REFERENCE   3  (bases 1 to 10041)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10041)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2003"; volume: "50"; issue: "1"; pages: "74-79"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-DEC-2002) Biosciences Division, Argonne National Laboratory, 
            9700 South Cass Ave., Argonne, IL 60439, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(276..987)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     CDS             complement(1246..1566)
                     /codon_start=1
                     /gene="lacZa"
                     /product="beta-galactosidase alpha peptide"
                     /label=lacZa
                     /protein_id="AAO47409.1"
                     /translation="MTMITPSLAAGRRIPGNSLAVVLQRRDWENPGVTQLNRLAAHPPF
                     ASWRNSEEARTDRPSQQLRSLNGEWRHASRREKGGQVSGKRQGRNRRAHEGASRGKRLV
                     SL"
     gene            complement(1246..1566)
                     /gene="lacZa"
                     /label=lacZa
     primer_bind     1497..1513
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1562..1578)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1586..1602
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1610..1640)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1655..1676)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1911..2279)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(2312..2421)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(3105..3752)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             3858..5054
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(6333..7478)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8205..9299)
                     /codon_start=1
                     /gene="incC"
                     /product="plasmid maintenance protein"
                     /label=incC
                     /protein_id="AAO47414.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            complement(8205..9299)
                     /gene="incC"
                     /label=incC
     CDS             complement(8981..9286)
                     /codon_start=1
                     /gene="korA"
                     /product="regulation of gene expression"
                     /label=korA
                     /protein_id="AAO47415.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            complement(8981..9286)
                     /gene="korA"
                     /label=korA
ORIGIN
        1 gaattcaccc ccgaacacga gcacggcacc cgcgaccact atgccaagaa tgcccaaggt
       61 aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg acggccgaag tgaagggcag
      121 gccgccaccc aggccgccgc cctcactgcc cggcacctgg tcgctgaatg tcgatgccag
      181 cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg ctgatcgccc atcccgttac
      241 tgccccgatc ccggcaatgg caaggactgc cagcgctgcc atttttgggg tgaggccgtt
      301 cgcggccgag gggcgcagcc cctgggggga tgggaggccc gcgttagcgg gccgggaggg
      361 ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct
      421 ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt
      481 ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct
      541 caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc
      601 gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc
      661 ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt
      721 gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg
      781 ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg
      841 ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg
      901 acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa
      961 ccagcccggt gagcgtcgga aaggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1021 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1081 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1141 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1201 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1261 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1321 tacctgtccg cctttctccc ttcgggaagc gtggcgccat tcgccattca ggctgcgcaa
     1381 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     1441 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     1501 aacgacggcc agtgaattcc cggggatccg tcgacctgca gccaagcttg gcgtaatcat
     1561 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1621 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1681 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1741 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcggtcttg ccttgctcgt
     1801 cggtgatgta cttcaccagc tccgcgaagt cgctcttctt gatggagcgc atggggacgt
     1861 gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca tggctctgcc
     1921 ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc ttcgatcttc
     1981 gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc gtcggtgagc
     2041 cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc cagctcgcgg
     2101 acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc cagcaggtag
     2161 gccgacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg ttcgtctgga
     2221 aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc atcagccatc
     2281 cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc aggattcccg
     2341 ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct cgcgggtggg
     2401 cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa gtctacacga
     2461 accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga aaaaatcgct
     2521 ataatgaccc cgaagcaggg ttatgcagcg gaaaagcgcc acgcttcccg aagggagaaa
     2581 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2641 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2701 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2761 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     2821 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     2881 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc agaaggccgc
     2941 cagagaggcc gagcgcggcc gtgaggcttg gacgctaggg cagggcatga aaaagcccgt
     3001 agcgggctgc tacgggcgtc tgacgcggtg gaaaggggga ggggatgttg tctacatggc
     3061 tctgctgtag tgagtgggtt gcgctccggc agcggtcctg atcaatcgtc accctttctc
     3121 ggtccttcaa cgttcctgac aacgagcctc cttttcgcca atccatcgac aatcaccgcg
     3181 agtccctgct cgaacgctgc gtccggaccg gcttcgtcga aggcgtctat cgcggcccgc
     3241 aacagcggcg agagcggagc ctgttcaacg gtgccgccgc gctcgccggc atcgctgtcg
     3301 ccggcctgct cctcaagcac ggccccaaca gtgaagtagc tgattgtcat cagcgcattg
     3361 acggcgtccc cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc gtcggccgtt
     3421 tccatctgcg gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc gcggtaggcg
     3481 agcagcgcct gcctgaagct gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg
     3541 gctctcggca ccgaatgcgt atgattctcc gccagcatgg cttcggccag tgcgtcgagc
     3601 agcgcccgct tgttcctgaa gtgccagtaa agcgccggct gctgaacccc caaccgttcc
     3661 gccagtttgc gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc cagggcggca
     3721 cggatcactg tattcggctg caactttgtc atgcttgaca ctttatcact gataaacata
     3781 atatgtccac caacttatca gtgataaaga atccgcgcgt tcaatcggac cagcggaggc
     3841 tggtccggag gccagacatg aaacccaaca tacccctgat cgtaattctg agcactgtcg
     3901 cgctcgacgc tgtcggcatc ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc
     3961 tggttcactc gaacgacgtc accgcccact atggcattct gctggcgctg tatgcgttgg
     4021 tgcaatttgc ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg cggcggccaa
     4081 tcttgctcgt ctcgctggcc ggcgccactg tcgactacgc catcatggcg acagcgcctt
     4141 tcctttgggt tctctatatc gggcggatcg tggccggcat caccggggcg actggggcgg
     4201 tagccggcgc ttatattgcc gatatcactg atggcgatga gcgcgcgcgg cacttcggct
     4261 tcatgagcgc ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg
     4321 gcggtttctc cccccacgct ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc
     4381 tgacgggctg tttccttttg ccggagtcgc acaaaggcga acgccggccg ttacgccggg
     4441 aggctctcaa cccgctcgct tcgttccggt gggcccgggg catgaccgtc gtcgccgccc
     4501 tgatggcggt cttcttcatc atgcaacttg tcggacaggt gccggccgcg ctttgggtca
     4561 ttttcggcga ggatcgcttt cactgggacg cgaccacgat cggcatttcg cttgccgcat
     4621 ttggcattct gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg
     4681 gcgaaaggcg ggcactcatg ctcggaatga ttgccgacgg cacaggctac atcctgcttg
     4741 ccttcgcgac acggggatgg atggcgttcc cgatcatggt cctgcttgct tcgggtggca
     4801 tcggaatgcc ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc
     4861 agctgcaagg ctcactggcg gcgctcacca gcctgacctc gatcgtcgga cccctcctct
     4921 tcacggcgat ctatgcggct tctataacaa cgtggaacgg gtgggcatgg attgcaggcg
     4981 ctgccctcta cttgctctgc ctgccggcgc tgcgtcgcgg gctttggagc ggcgcagggc
     5041 aacgagccga tcgctgatcg tggaaacgat aggcctatgc catgcgggtc aaggcgactt
     5101 ccggcaagct atacgcgccc taggagtgcg gttggaacgt tggcccagcc agatactccc
     5161 gatcacgagc aggacgccga tgatttgaag cgcactcagc gtctgatcca agaacaacca
     5221 tcctagcaac acggcggtcc ccgggctgag aaagcccagt aaggaaacaa ctgtaggttc
     5281 gagtcgcgag atcccccgga accaaaggaa gtaggttaaa cccgctccga tcaggccgag
     5341 ccacgccagg ccgagaacat tggttcctgt aggcatcggg attggcggat caaacactaa
     5401 agctactgga acgagcagaa gtcctccggc cgccagttgc caggcggtaa aggtgagcag
     5461 aggcacggga ggttgccact tgcgggtcag cacggttccg aacgccatgg aaaccgcccc
     5521 cgccaggccc gctgcgacgc cgacaggatc tagcgctgcg tttggtgtca acaccaacag
     5581 cgccacgccc gcagttccgc aaatagcccc caggaccgcc atcaatcgta tcgggctacc
     5641 tagcagagcg gcagagatga acacgaccat cagcggctgc acagcgccta ccgtcgccgc
     5701 gaccccgccc ggcaggcggt agaccgaaat aaacaacaag ctccagaata gcgaaatatt
     5761 aagtgcgccg aggatgaaga tgcgcatcca ccagattccc gttggaatct gtcggacgat
     5821 catcacgagc aataaacccg ccggcaacgc ccgcagcagc ataccggcga cccctcggcc
     5881 tcgctgttcg ggctccacga aaacgccgga cagatgcgcc ttgtgagcgt ccttggggcc
     5941 gtcctcctgt ttgaagaccg acagcccaat gatctcgccg tcgatgtagg cgccgaatgc
     6001 cacggcatct cgcaaccgtt cagcgaacgc ctccatgggc tttttctcct cgtgctcgta
     6061 aacggacccg aacatctctg gagctttctt cagggccgac aatcggatct cgcggaaatc
     6121 ctgcacgtcg gccgctccaa gccgtcgaat ctgagcctta atcacaattg tcaattttaa
     6181 tcctctgttt atcggcagtt cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag
     6241 caagtgcgtc gagcagtgcc cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa
     6301 cccccagccg gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg
     6361 acccaggcgt gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc
     6421 tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc
     6481 ttgagcgggt acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc
     6541 gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg
     6601 acgatttcct cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg
     6661 cggaagcggt gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc
     6721 atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg
     6781 atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata
     6841 ggggtgcgct tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc
     6901 agctcgacgc cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt
     6961 tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg
     7021 tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc
     7081 atttccttga tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc
     7141 tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg
     7201 tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc
     7261 acggcggccg atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc
     7321 ttggccgtag cttgctggac catcgagccg acggactgga aggtttcgcg gggcgcacgc
     7381 atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt
     7441 tcttgcctgt atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc
     7501 ccgactcacg ccggggcaat gtgcccttat tcctgatttg acccgcctgg tgccttggtg
     7561 tccagataat ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc
     7621 tcgtacttgg tattccgaat cttgccctgc acgaatacca gcgacccctt gcccaaatac
     7681 ttgccgtggg cctcggcctg agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc
     7741 tgcttgtcgc cggtcgtggc cgcgccaacc tttgcgatcc gcaagcgcgc ggtcgccatc
     7801 ttcacgctgg aacagtacgt cgaggcgggc atcatgaccc gcgagcaata cgaggtcatt
     7861 aaaagcgccg tgattgatga tatagcggcc cggctgctcc tggttctcgc gcaccgaaat
     7921 gggtgacttc accccgcgct ctttgatcgt ggcaccgatt tccgcgatgc tctccgggga
     7981 aaagccgggg ttgtcggccg tccgcggctg atgcggatct tcgtcgatca ggtccaggtc
     8041 cagctcgata gggccggaac cgccctgaga cgccgcagga gcgtccagga ggctcgacag
     8101 gtcgccgatg ctatccaacc ccaggccgga cggctgcgcc gcgcctgcgg cttcctgagc
     8161 ggccgcagcg gtgtttttct tggtggtctt ggcttgagcc gcagtcattg ggaaatctcc
     8221 atcttcgtga acacgtaatc agccagggcg cgaacctctt tcgatgcctt gcgcgcggcc
     8281 gttttcttga tcttccagac cggcacaccg gatgcgaggg catcggcgat gctgctgcgc
     8341 aggccaacgg tggccggaat catcatcttg gggtacgcgg ccagcagctc ggcttggtgg
     8401 cgcgcgtggc gcggattccg cgcatcgacc ttgctgggca ccatgccaag gaattgcagc
     8461 ttggcgttct tctggcgcac gttcgcaatg gtcgtgacca tcttcttgat gccctggatg
     8521 ctgtacgcct caagctcgat gggggacagc acatagtcgg ccgcgaagag ggcggccgcc
     8581 aggccgacgc caagggtcgg ggccgtgtcg atcaggcaca cgtcgaagcc ttggttcgcc
     8641 agggccttga tgttcgcccc gaacagctcg cgggcgtcgt ccagcgacag ccgttcggcg
     8701 ttcgccagta ccgggttgga ctcgatgagg gcgaggcgcg cggcctggcc gtcgccggct
     8761 gcgggtgcgg tttcggtcca gccgccggca gggacagcgc cgaacagctt gcttgcatgc
     8821 aggccggtag caaagtcctt gagcgtgtag gacgcattgc cctgggggtc caggtcgatc
     8881 acggcaaccc gcaagccgcg ctcgaaaaag tcgaaggcaa gatgcacaag ggtcgaagtc
     8941 ttgccgacgc cgcctttctg gttggccgtg accaaagttt tcatcgtttg gtttcctgtt
     9001 ttttcttggc gtccgcttcc cacttccgga cgatgtacgc ctgatgttcc ggcagaaccg
     9061 ccgttacccg cgcgtacccc tcgggcaagt tcttgtcctc gaacgcggcc cacacgcgat
     9121 gcaccgcttg cgacactgcg cccctggtca gtcccagcga cgttgcgaac gtcgcctgtg
     9181 gcttcccatc gactaagacg ccccgcgcta tctcgatggt ctgctgcccc acttccagcc
     9241 cctggatcgc ctcctggaac tggctttcgg taagccgttt cttcatggat aacacccata
     9301 atttgctccg cgccttggtt gaacatagcg gtgacagccg ccagcacatg agagaagttt
     9361 agctaaacat ttctcgcacg tcaacacctt tagccgctaa aactcgtcct tggcgtaaca
     9421 aaacaaaagc ccggaaaccg ggctttcgtc tcttgccgct tatggctctg cacccggctc
     9481 catcaccaac aggtcgcgca cgcgcttcac tcggttgcgg atcgacactg ccagcccaac
     9541 aaagccggtt gccgccgccg ccaggatcgc gccgatgatg ccggccacac cggccatcgc
     9601 ccaccaggtc gccgccttcc ggttccattc ctgctggtac tgcttcgcaa tgctggacct
     9661 cggctcacca taggctgacc gctcgatggc gtatgccgct tctccccttg gcgtaaaacc
     9721 cagcgccgca ggcggcattg ccatgctgcc cgccgctttc ccgaccacga cgcgcgcacc
     9781 aggcttgcgg tccagacctt cggccacggc gagctgcgca aggacataat cagccgccga
     9841 cttggctcca cgcgcctcga tcagctcttg cactcgcgcg aaatccttgg cctccacggc
     9901 cgccatgaat cgcgcacgcg gcgaaggctc cgcagggccg gcgtcgtgat cgccgccgag
     9961 aatgcccttc accaagttcg acgacacgaa aatcatgctg acggctatca ccatcatgca
    10021 gacggatcgc acgaacccgc t
//
