LOCUS       40924_37128       11912 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad host range expression vector pRK415iq, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11912)
  AUTHORS   Kickstein E, Harms K, Wackernagel W.
  TITLE     Deletions of recBCD or recD influence genetic transformation 
            differently and are lethal together with a recJ deletion in 
            Acinetobacter baylyi
  JOURNAL   Microbiology (Reading, Engl.) 153 (PT 7), 2259-2270 (2007)
  PUBMED    17600070
REFERENCE   2  (bases 1 to 11912)
  AUTHORS   Harms K, Huelter N, de Vries J, Wackernagel W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2007) Genetics, Department of Biology and 
            Environmental Sciences, University of Oldenburg, 
            Carl-von-Ossietzky-Strasse 9-11, Oldenburg D-26111, Germany
REFERENCE   3  (bases 1 to 11912)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11912)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology (Reading, Engl.) 153 (PT 7), 2259-2270 (2007)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2007) Genetics, Department of Biology and Environmental 
            Sciences, University of Oldenburg, Carl-von-Ossietzky-Strasse 9-11, 
            Oldenburg D-26111, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..712)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     primer_bind     1222..1238
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1239..1295
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(1308..1324)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1332..1348)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1356..1386)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1401..1422)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1657..2025)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(2058..2167)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(2851..3498)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             3604..4800
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     promoter        4840..4917
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4918..5997
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     CDS             complement(6656..7321)
                     /codon_start=1
                     /gene="upf16.5"
                     /product="unknown plasmid factor 16.5"
                     /label=upf16.5
                     /protein_id="ABO32169.1"
                     /translation="MTWCIANARALWGQFRLGVQQPALYWHFRNKRALLDALASLSIAR
                     AHGALYELPINRGLKLTIVIKAQIRRLGAADVQDFREIRLSALKKAPEMFGSVYEHEEK
                     KPMEAFAERLRDAVAFGAYIDGEIIGLSVFKQEDGPKDAHKAHLSGVFVEPEQRGRGVA
                     GMLLRALPAGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGSRRR"
     gene            complement(6656..7321)
                     /gene="upf16.5"
                     /label=upf16.5
     CDS             complement(7299..8444)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(8493..8843)
                     /codon_start=1
                     /gene="ssb"
                     /product="single-strand binding protein"
                     /label=ssb
                     /protein_id="ABO32172.1"
                     /translation="MSHNQFQFIGNLTRDTEVRHGNSNKPQAIFDIAVNEEWRNDAGDK
                     QERTDFFRIKCFGSQAEAHGKYLGKGSLVFVQGKIRNTKYEKDGQTVYGTDFIADKVDY
                     LDTKAPGGSNQE"
     gene            complement(8493..8843)
                     /gene="ssb"
                     /label=ssb
     CDS             8964..9329
                     /codon_start=1
                     /gene="trbA"
                     /product="TrbA"
                     /label=trbA
                     /note="regulation of tra gene expression"
                     /protein_id="ABO32173.1"
                     /translation="MYNQIFFTNILRLLDERGMTKHELSERAGVSISFLSDLTNGKANP
                     SLKVMEAIADALETPLPLLLESTDLDREALAEIAGHPFKSSVPPGYERISVVLPSHKAF
                     IVKKWGDDTRKKLRGRL"
     gene            8964..9329
                     /gene="trbA"
                     /label=trbA
     CDS             complement(10367..11461)
                     /codon_start=1
                     /gene="incC1"
                     /product="IncC1"
                     /label=incC1
                     /note="IncC partitioning protein, long form"
                     /protein_id="ABO32174.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            complement(10367..11461)
                     /gene="incC1"
                     /label=incC1
     CDS             complement(10367..11146)
                     /codon_start=1
                     /gene="incC2"
                     /product="IncC2"
                     /label=incC2
                     /note="IncC partitioning protein, short form"
                     /protein_id="ABO32175.1"
                     /translation="MKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNAS
                     YTLKDFATGLHASKLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDA
                     RELFGANIKALANQGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMV
                     TTIANVRQKNAKLQFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADA
                     LASGVPVWKIKKTAARKASKEVRALADYVFTKMEISQ"
     gene            complement(10367..11146)
                     /gene="incC2"
                     /label=incC2
     CDS             complement(11143..11448)
                     /codon_start=1
                     /gene="korA"
                     /product="KorA"
                     /label=korA
                     /note="regulation of IncC repression"
                     /protein_id="ABO32176.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            complement(11143..11448)
                     /gene="korA"
                     /label=korA
ORIGIN
        1 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
       61 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
      121 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
      181 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
      241 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
      301 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
      361 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
      421 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
      481 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
      541 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
      601 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
      661 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gctcttccgc
      721 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      781 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      841 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      901 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      961 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1021 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1081 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     1141 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     1201 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtacccggg
     1261 gatcctctag agtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt
     1321 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1381 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1441 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1501 gggagaggcg gtttgcgtat tgggcgctcg gtcttgcctt gctcgtcggt gatgtacttc
     1561 accagctccg cgaagtcgct cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg
     1621 cgcacccccc ggccgtttta gcggctaaaa aagtcatggc tctgccctcg ggcggaccac
     1681 gcccatcatg accttgccaa gctcgtcctg cttctcttcg atcttcgcca gcagggcgag
     1741 gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag
     1801 gccgcccagg cggcccaggt cgccattgat gcgggccagc tcgcggacgt gctcatagtc
     1861 cacgacgccc gtgattttgt agccctggcc gacggccagc aggtaggccg acaggctcat
     1921 gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt
     1981 gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc
     2041 tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag
     2101 gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat
     2161 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa
     2221 tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa
     2281 gcagggttat gcagcggaaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2341 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2401 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2461 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2521 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2581 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2641 gcagcgagtc agtgagcgag gaagcggaag agcgccagaa ggccgccaga gaggccgagc
     2701 gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa gcccgtagcg ggctgctacg
     2761 ggcgtctgac gcggtggaaa gggggagggg atgttgtcta catggctctg ctgtagtgag
     2821 tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc tttctcggtc cttcaacgtt
     2881 cctgacaacg agcctccttt tcgccaatcc atcgacaatc accgcgagtc cctgctcgaa
     2941 cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg gcccgcaaca gcggcgagag
     3001 cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg ctgtcgccgg cctgctcctc
     3061 aagcacggcc ccaacagtga agtagctgat tgtcatcagc gcattgacgg cgtccccggc
     3121 cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc
     3181 gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct
     3241 gaagctgcgg gcattcccga tcagaaatga gcgccagtcg tcgtcggctc tcggcaccga
     3301 atgcgtatga ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt
     3361 cctgaagtgc cagtaaagcg ccggctgctg aacccccaac cgttccgcca gtttgcgtgt
     3421 cgtcagaccg tctacgccga cctcgttcaa caggtccagg gcggcacgga tcactgtatt
     3481 cggctgcaac tttgtcatgc ttgacacttt atcactgata aacataatat gtccaccaac
     3541 ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca
     3601 gacatgaaac ccaacatacc cctgatcgta attctgagca ctgtcgcgct cgacgctgtc
     3661 ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac
     3721 gacgtcaccg cccactatgg cattctgctg gcgctgtatg cgttggtgca atttgcctgc
     3781 gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc ggccaatctt gctcgtctcg
     3841 ctggccggcg ccactgtcga ctacgccatc atggcgacag cgcctttcct ttgggttctc
     3901 tatatcgggc ggatcgtggc cggcatcacc ggggcgactg gggcggtagc cggcgcttat
     3961 attgccgata tcactgatgg cgatgagcgc gcgcggcact tcggcttcat gagcgcctgt
     4021 ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg tttctccccc
     4081 cacgctccgt tcttcgccgc ggcagccttg aacggcctca atttcctgac gggctgtttc
     4141 cttttgccgg agtcgcacaa aggcgaacgc cggccgttac gccgggaggc tctcaacccg
     4201 ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg ccgccctgat ggcggtcttc
     4261 ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt cggcgaggat
     4321 cgctttcact gggacgcgac cacgatcggc atttcgcttg ccgcatttgg cattctgcat
     4381 tcactcgccc aggcaatgat caccggccct gtagccgccc ggctcggcga aaggcgggca
     4441 ctcatgctcg gaatgattgc cgacggcaca ggctacatcc tgcttgcctt cgcgacacgg
     4501 ggatggatgg cgttcccgat catggtcctg cttgcttcgg gtggcatcgg aatgccggcg
     4561 ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc aggggcagct gcaaggctca
     4621 ctggcggcgc tcaccagcct gacctcgatc gtcggacccc tcctcttcac ggcgatctat
     4681 gcggcttcta taacaacgtg gaacgggtgg gcatggattg caggcgctgc cctctacttg
     4741 ctctgcctgc cggcgctgcg tcgcgggctt tggagcggcg cagggcaacg agccgatcgc
     4801 tgatcgtgga aacgataggt taatttctag atttacgttg acaccatcga atggtgcaaa
     4861 acctttcgcg gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg
     4921 aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc
     4981 cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg
     5041 atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg
     5101 ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg
     5161 gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga
     5221 agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg
     5281 ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact
     5341 aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc
     5401 tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa
     5461 atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg
     5521 cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt
     5581 gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg
     5641 atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg
     5701 ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt
     5761 tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg
     5821 gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc
     5881 tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg
     5941 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     6001 gcgcaacgca attaatgtaa gttagcgcgt ctagaatata tcctatgcca tgcgggtcaa
     6061 ggcgacttcc ggcaagctat acgcgcccta ggagtgcggt tggaacgttg gcccagccag
     6121 atactcccga tcacgagcag gacgccgatg atttgaagcg cactcagcgt ctgatccaag
     6181 aacaaccatc ctagcaacac ggcggtcccc gggctgagaa agcccagtaa ggaaacaact
     6241 gtaggttcga gtcgcgagat cccccggaac caaaggaagt aggttaaacc cgctccgatc
     6301 aggccgagcc acgccaggcc gagaacattg gttcctgtag gcatcgggat tggcggatca
     6361 aacactaaag ctactggaac gagcagaagt cctccggccg ccagttgcca ggcggtaaag
     6421 gtgagcagag gcacgggagg ttgccacttg cgggtcagca cggttccgaa cgccatggaa
     6481 accgcccccg ccaggcccgc tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac
     6541 accaacagcg ccacgcccgc agttccgcaa atagccccca ggaccgccat caatcgtatc
     6601 gggctaccta gcagagcggc agagatgaac acgaccatca gcggctgcac agcgcctacc
     6661 gtcgccgcga cccgcccggc aggcggtaga ccgaaataaa caacaagctc cagaatagcg
     6721 aaatattaag tgcgccgagg atgaagatgc gcatccacca gattcccgtt ggaatctgtc
     6781 ggacgatcat cacgagcaat aaacccgccg gcaacgcccg cagcagcata ccggcgaccc
     6841 ctcggcctcg ctgttcgggc tccacgaaaa cgccggacag atgcgccttg tgagcgtcct
     6901 tggggccgtc ctcctgtttg aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc
     6961 cgaatgccac ggcatctcgc aaccgttcag cgaacgcctc catgggcttt ttctcctcgt
     7021 gctcgtaaac ggacccgaac atctctggag ctttcttcag ggccgacaat cggatctcgc
     7081 ggaaatcctg cacgtcggcc gctccaagcc gtcgaatctg agccttaatc acaattgtca
     7141 attttaatcc tctgtttatc ggcagttcgt agagcgcgcc gtgcgcccga gcgatactga
     7201 gcgaagcaag tgcgtcgagc agtgcccgct tgttcctgaa atgccagtaa agcgctggct
     7261 gctgaacccc cagccggaac tgaccccaca aggccctagc gtttgcaatg caccaggtca
     7321 tcattgaccc aggcgtgttc caccaggccg ctgcctcgca actcttcgca ggcttcgccg
     7381 acctgctcgc gccacttctt cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt
     7441 tccagcttga gcgggtacgg ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc
     7501 gtcggcgaca gcttgcggta cttctcccat atgaatttcg tgtagtggtc gccagcaaac
     7561 agcacgacga tttcctcgtc gatcaggacc tggcaacggg acgttttctt gccacggtcc
     7621 aggacgcgga agcggtgcag cagcgacacc gattccaggt gcccaacgcg gtcggacgtg
     7681 aagcccatcg ccgtcgcctg taggcgcgac aggcattcct cggccttcgt gtaataccgg
     7741 ccattgatcg accagcccag gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg
     7801 ccgatagggg tgcgcttcgc gtactccaac acctgctgcc acaccagttc gtcatcgtcg
     7861 gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc
     7921 ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg
     7981 gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa
     8041 agctgcattt ccttgatctg ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg
     8101 ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct tcttggtcgt catagttcct
     8161 cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa
     8221 cgctccacgg cggccgatgg cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc
     8281 tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg actggaaggt ttcgcggggc
     8341 gcacgcatga cggtgcggct tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg
     8401 atcagttctt gcctgtatgc cttccggtca aacgtccgat tcattcaccc tccttgcggg
     8461 attgccccga ctcacgccgg ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc
     8521 ttggtgtcca gataatccac cttatcggca atgaagtcgg tcccgtagac cgtctggccg
     8581 tccttctcgt acttggtatt ccgaatcttg ccctgcacga ataccagcga ccccttgccc
     8641 aaatacttgc cgtgggcctc ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg
     8701 cgctcctgct tgtcgccggc atcgttgcgc cactcttcat taaccgctat atcgaaaatt
     8761 gcttgcggct tgttagaatt gccatgacgt acctcggtgt cacgggtaag attaccgata
     8821 aactggaact gattatggct catatcgaaa gtctccttga gaaaggagac tctagtttag
     8881 ctaaacattg gttccgctgt caagaacttt agcggctaaa attttgcggg ccgcgaccaa
     8941 aggtgcgagg ggcggcttcc gctgtgtaca accagatatt tttcaccaac atccttcgtc
     9001 tgctcgatga gcggggcatg acgaaacatg agctgtcgga gagggcaggg gtttcaattt
     9061 cgtttttatc agacttaacc aacggtaagg ccaacccctc gttgaaggtg atggaggcca
     9121 ttgccgacgc cctggaaact cccctacctc ttctcctgga gtccaccgac cttgaccgcg
     9181 aggcactcgc ggagattgcg ggtcatcctt tcaagagcag cgtgccgccc ggatacgaac
     9241 gcatcagtgt ggttttgccg tcacataagg cgtttatcgt aaagaaatgg ggcgacgaca
     9301 cccgaaaaaa gctgcgtgga aggctctgac gccaagggtt agggcttgca cttccttctt
     9361 tagccgctaa aacggcccct tctctgcggg ccgtcggctc gcgcatcata tcgacatcct
     9421 caacggaagc cgtgccgcga atggcatcgg gcgggtgcgc tttgacagtt gttttctatc
     9481 agaaccccta cgtcgtgcgg ttcgattagc tgtttgtctt gcaggctaaa cactttcggt
     9541 atatcgtttg cctgtgcgat aatgttgcta atgatttgtt gcgtaggggt tactgaaaag
     9601 tgagcgggaa agaagagttt cagaccatca aggagcgggc caagcgcaag ctggaacgcg
     9661 acatgggtgc ggacctgttg gccgcgctca acgacccgaa aaccgttgaa gtcatgctca
     9721 acgcggacgg caaggtgtgg cacgaacgcc ttggcgagcc gatgcggtac atctgcgaca
     9781 tgcggcccag ccagtcgcag gcgattatag aaacggtggc cggattccac ggcaaagagg
     9841 tcacgcggca ttcgcccatc ctggaaggcg agttcccctt ggatggcagc cgctttgccg
     9901 gccaattgcc gccggtcgtg gccgcgccaa cctttgcgat ccgcaagcgc gcggtcgcca
     9961 tcttcacgct ggaacagtac gtcgaggcgg gcatcatgac ccgcgagcaa tacgaggtca
    10021 ttaaaagcgc cgtgattgat gatatagcgg cccggctgct cctggttctc gcgcaccgaa
    10081 atgggtgact tcaccccgcg ctctttgatc gtggcaccga tttccgcgat gctctccggg
    10141 gaaaagccgg ggttgtcggc cgtccgcggc tgatgcggat cttcgtcgat caggtccagg
    10201 tccagctcga tagggccgga accgccctga gacgccgcag gagcgtccag gaggctcgac
    10261 aggtcgccga tgctatccaa ccccaggccg gacggctgcg ccgcgcctgc ggcttcctga
    10321 gcggccgcag cggtgttttt cttggtggtc ttggcttgag ccgcagtcat tgggaaatct
    10381 ccatcttcgt gaacacgtaa tcagccaggg cgcgaacctc tttcgatgcc ttgcgcgcgg
    10441 ccgttttctt gatcttccag accggcacac cggatgcgag ggcatcggcg atgctgctgc
    10501 gcaggccaac ggtggccgga atcatcatct tggggtacgc ggccagcagc tcggcttggt
    10561 ggcgcgcgtg gcgcggattc cgcgcatcga ccttgctggg caccatgcca aggaattgca
    10621 gcttggcgtt cttctggcgc acgttcgcaa tggtcgtgac catcttcttg atgccctgga
    10681 tgctgtacgc ctcaagctcg atgggggaca gcacatagtc ggccgcgaag agggcggccg
    10741 ccaggccgac gccaagggtc ggggccgtgt cgatcaggca cacgtcgaag ccttggttcg
    10801 ccagggcctt gatgttcgcc ccgaacagct cgcgggcgtc gtccagcgac agccgttcgg
    10861 cgttcgccag taccgggttg gactcgatga gggcgaggcg cgcggcctgg ccgtcgccgg
    10921 ctgcgggtgc ggtttcggtc cagccgccgg cagggacagc gccgaacagc ttgcttgcat
    10981 gcaggccggt agcaaagtcc ttgagcgtgt aggacgcatt gccctggggg tccaggtcga
    11041 tcacggcaac ccgcaagccg cgctcgaaaa agtcgaaggc aagatgcaca agggtcgaag
    11101 tcttgccgac gccgcctttc tggttggccg tgaccaaagt tttcatcgtt tggtttcctg
    11161 ttttttcttg gcgtccgctt cccacttccg gacgatgtac gcctgatgtt ccggcagaac
    11221 cgccgttacc cgcgcgtacc cctcgggcaa gttcttgtcc tcgaacgcgg cccacacgcg
    11281 atgcaccgct tgcgacactg cgcccctggt cagtcccagc gacgttgcga acgtcgcctg
    11341 tggcttccca tcgactaaga cgccccgcgc tatctcgatg gtctgctgcc ccacttccag
    11401 cccctggatc gcctcctgga actggctttc ggtaagccgt ttcttcatgg ataacaccca
    11461 taatttgctc cgcgccttgg ttgaacatag cggtgacagc cgccagcaca tgagagaagt
    11521 ttagctaaac atttctcgca cgtcaacacc tttagccgct aaaactcgtc cttggcgtaa
    11581 caaaacaaaa gcccggaaac cgggctttcg tctcttgccg cttatggctc tgcacccggc
    11641 tccatcacca acaggtcgcg cacgcgcttc actcggttgc ggatcgacac tgccagccca
    11701 acaaagccgg ttgccgccgc cgccaggatc gcgccgatga tgccggccac accggccatc
    11761 gcccaccagg tcgccgccct cactgcccgg cacctggtcg ctgaatgtcg atgccagcac
    11821 ctgcggcacg tcaatgcttc cgggcgtcgc gctcgggctg atcgcccatc ccgttactgc
    11881 cccgatcccg gcaatggcaa ggactgccag cg
//
