LOCUS       Exported               10692 bp DNA     circular SYN 08-SEP-2025
DEFINITION  Broad host range expression vector pRK415, complete sequence.
ACCESSION   EF437940
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Hulter N, Wackernagel W.
  TITLE     Double illegitimate recombination events integrate DNA segments 
            through two different mechanisms during natural transformation of 
            Acinetobacter baylyi
  JOURNAL   Mol. Microbiol. 67 (5), 984-995 (2008)
  PUBMED    18194157
REFERENCE   2  (bases 1 to 10692)
  AUTHORS   Huelter N, Wackernagel W.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-FEB-2007) Genetics, Department of Biology and 
            Environmental Sciences, Carl von Ossietzky University Oldenburg, 
            Carl-von-Ossietzkystrasse 9-11, Oldenburg 26111, Germany
REFERENCE   3  (bases 1 to 10692)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Microbiol."; date: "2008"; volume: "67"; issue: "5"; pages: 
            "984-995"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-FEB-2007) Genetics, Department of Biology and Environmental 
            Sciences, Carl von Ossietzky University Oldenburg, 
            Carl-von-Ossietzkystrasse 9-11, Oldenburg 26111, Germany"
FEATURES             Location/Qualifiers
     source          1..10692
                     /mol_type="other DNA"
                     /note="derived from plasmid pRK404 GenBank Accession Number
                     AY204475"
                     /db_xref="taxon:429723"
                     /organism="Broad host range expression vector pRK415"
     rep_origin      1..712
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      276..987
                     /note="oriV; IncP"
     CDS             complement(971..1312)
                     /codon_start=1
                     /gene="lacZ-alpha"
                     /product="beta-galactosidase alpha peptide"
                     /label=lacZ-alpha
                     /protein_id="ABO32178.1"
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRHASRREKGGQVSGKRQGRNRRAHEGA
                     SRGKRLVSL"
     gene            complement(971..1312)
                     /gene="lacZ-alpha"
                     /label=lacZ-alpha
     primer_bind     1222..1238
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1239..1295
                     /label=multiple cloning site
                     /note="multiple cloning site"
     primer_bind     complement(1308..1324)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1332..1348
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      1352..1421
                     /label=lac promoter
                     /note="lac promoter"
                     /regulatory_class="promoter"
     promoter        complement(1356..1386)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1401..1422
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     gene            complement(1654..2279)
                     /gene="traJ"
                     /label=traJ
     CDS             complement(1654..2025)
                     /codon_start=1
                     /gene="traJ"
                     /product="TraJ"
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /protein_id="ABO32179.1"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            2058..2167
                     /label=incP
                     /note="incP"
     misc_feature    2299..2532
                     /label=similar to NC_001371 Plasmid ColE1
                     /note="similar to NC_001371 Plasmid ColE1"
     misc_feature    2524..2676
                     /label=similar to NC_001371 Plasmid ColE1
                     /note="similar to NC_001371 Plasmid ColE1"
     CDS             complement(2848..3498)
                     /codon_start=1
                     /gene="tetR"
                     /product="TetR"
                     /label=tetR
                     /note="tetracycline resistance regulation"
                     /protein_id="ABO32180.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(2848..3498)
                     /gene="tetR"
                     /label=tetR
     CDS             3604..4803
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline resistance protein"
                     /label=tetA
                     /protein_id="ABO32181.1"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     gene            3604..4803
                     /gene="tetA"
                     /label=tetA
     CDS             complement(4835..5911)
                     /codon_start=1
                     /label=upf16.5
                     /translation="MIKAQIRRLGAADVQDFREIRLSALKKAPEMFGSVYEHEEKKPME
                     AFAERLRDAVAFGAYIDGEIIGLSVFKQEDGPKDAHKAHLSGVFVEPEQRGRGVAGMLL
                     RALPAGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQ
                     PLMVVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAF
                     GTVLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGA
                     GLTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(5434..6101)
                     /gene="upf16.5"
                     /label=upf16.5
     CDS             complement(6076..7224)
                     /codon_start=1
                     /gene="trfA2"
                     /product="trans-acting replication A2"
                     /label=trfA2
                     /protein_id="ABO32183.1"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     gene            complement(6076..7224)
                     /gene="trfA2"
                     /label=trfA2
     CDS             complement(6076..6933)
                     /codon_start=1
                     /gene="trfA1"
                     /product="trans-acting replication A1"
                     /label=trfA1
                     /protein_id="ABO32184.1"
                     /translation="MTTKKRKTAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAP
                     WPDTMRAMPNDTARSALFTTRNKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELV
                     WQQVLEYAKRTPIGEPITFTFYELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRV
                     GHLESVSLLHRFRVLDRGKKTSRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRM
                     FDYFSSHREPYPLKLETFRLMCGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLV
                     HCKR"
     gene            complement(6076..6933)
                     /gene="trfA1"
                     /label=trfA1
     CDS             complement(7273..7623)
                     /codon_start=1
                     /gene="ssb"
                     /product="single-stranded DNA binding protein"
                     /label=ssb
                     /protein_id="ABO32185.1"
                     /translation="MSHNQFQFIGNLTRDTEVRHGNSNKPQAIFDIAVNEEWRNDAGDK
                     QERTDFFRIKCFGSQAEAHGKYLGKGSLVFVQGKIRNTKYEKDGQTVYGTDFIADKVDY
                     LDTKAPGGSNQE"
     gene            complement(7273..7623)
                     /gene="ssb"
                     /label=ssb
     CDS             7744..8109
                     /codon_start=1
                     /gene="trbA"
                     /product="TrbA"
                     /label=trbA
                     /note="regulation of tra gene expression"
                     /protein_id="ABO32186.1"
                     /translation="MYNQIFFTNILRLLDERGMTKHELSERAGVSISFLSDLTNGKANP
                     SLKVMEAIADALETPLPLLLESTDLDREALAEIAGHPFKSSVPPGYERISVVLPSHKAF
                     IVKKWGDDTRKKLRGRL"
     gene            7744..8109
                     /gene="trbA"
                     /label=trbA
     CDS             complement(9147..10241)
                     /codon_start=1
                     /gene="incC1"
                     /product="IncC1"
                     /label=incC1
                     /note="IncC partitioning protein, long form"
                     /protein_id="ABO32187.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            complement(9147..10241)
                     /gene="incC1"
                     /label=incC1
     CDS             complement(9147..9926)
                     /codon_start=1
                     /gene="incC2"
                     /product="IncC2"
                     /label=incC2
                     /note="IncC partitioning protein, short form"
                     /protein_id="ABO32188.1"
                     /translation="MKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNAS
                     YTLKDFATGLHASKLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDA
                     RELFGANIKALANQGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMV
                     TTIANVRQKNAKLQFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADA
                     LASGVPVWKIKKTAARKASKEVRALADYVFTKMEISQ"
     gene            complement(9147..9926)
                     /gene="incC2"
                     /label=incC2
     CDS             complement(9923..10228)
                     /codon_start=1
                     /gene="korA"
                     /product="KorA"
                     /label=korA
                     /note="regulation of gene expression"
                     /protein_id="ABO32189.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            complement(9923..10228)
                     /gene="korA"
                     /label=korA
ORIGIN
        1 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
       61 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
      121 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
      181 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
      241 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
      301 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
      361 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
      421 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
      481 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
      541 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
      601 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
      661 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gctcttccgc
      721 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      781 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      841 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      901 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      961 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1021 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1081 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     1141 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     1201 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtacccggg
     1261 gatcctctag agtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt
     1321 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     1381 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     1441 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     1501 gggagaggcg gtttgcgtat tgggcgctcg gtcttgcctt gctcgtcggt gatgtacttc
     1561 accagctccg cgaagtcgct cttcttgatg gagcgcatgg ggacgtgctt ggcaatcacg
     1621 cgcacccccc ggccgtttta gcggctaaaa aagtcatggc tctgccctcg ggcggaccac
     1681 gcccatcatg accttgccaa gctcgtcctg cttctcttcg atcttcgcca gcagggcgag
     1741 gatcgtggca tcaccgaacc gcgccgtgcg cgggtcgtcg gtgagccaga gtttcagcag
     1801 gccgcccagg cggcccaggt cgccattgat gcgggccagc tcgcggacgt gctcatagtc
     1861 cacgacgccc gtgattttgt agccctggcc gacggccagc aggtaggccg acaggctcat
     1921 gccggccgcc gccgcctttt cctcaatcgc tcttcgttcg tctggaaggc agtacacctt
     1981 gataggtggg ctgcccttcc tggttggctt ggtttcatca gccatccgct tgccctcatc
     2041 tgttacgccg gcggtagccg gccagcctcg cagagcagga ttcccgttga gcaccgccag
     2101 gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat
     2161 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa
     2221 tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa
     2281 gcagggttat gcagcggaaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2341 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2401 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2461 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2521 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2581 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2641 gcagcgagtc agtgagcgag gaagcggaag agcgccagaa ggccgccaga gaggccgagc
     2701 gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa gcccgtagcg ggctgctacg
     2761 ggcgtctgac gcggtggaaa gggggagggg atgttgtcta catggctctg ctgtagtgag
     2821 tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc tttctcggtc cttcaacgtt
     2881 cctgacaacg agcctccttt tcgccaatcc atcgacaatc accgcgagtc cctgctcgaa
     2941 cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg gcccgcaaca gcggcgagag
     3001 cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg ctgtcgccgg cctgctcctc
     3061 aagcacggcc ccaacagtga agtagctgat tgtcatcagc gcattgacgg cgtccccggc
     3121 cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc
     3181 gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct
     3241 gaagctgcgg gcattcccga tcagaaatga gcgccagtcg tcgtcggctc tcggcaccga
     3301 atgcgtatga ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt
     3361 cctgaagtgc cagtaaagcg ccggctgctg aacccccaac cgttccgcca gtttgcgtgt
     3421 cgtcagaccg tctacgccga cctcgttcaa caggtccagg gcggcacgga tcactgtatt
     3481 cggctgcaac tttgtcatgc ttgacacttt atcactgata aacataatat gtccaccaac
     3541 ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca
     3601 gacgtgaaac ccaacatacc cctgatcgta attctgagca ctgtcgcgct cgacgctgtc
     3661 ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac
     3721 gacgtcaccg cccactatgg cattctgctg gcgctgtatg cgttggtgca atttgcctgc
     3781 gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc ggccaatctt gctcgtctcg
     3841 ctggccggcg ccactgtcga ctacgccatc atggcgacag cgcctttcct ttgggttctc
     3901 tatatcgggc ggatcgtggc cggcatcacc ggggcgactg gggcggtagc cggcgcttat
     3961 attgccgata tcactgatgg cgatgagcgc gcgcggcact tcggcttcat gagcgcctgt
     4021 ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg tttctccccc
     4081 cacgctccgt tcttcgccgc ggcagccttg aacggcctca atttcctgac gggctgtttc
     4141 cttttgccgg agtcgcacaa aggcgaacgc cggccgttac gccgggaggc tctcaacccg
     4201 ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg ccgccctgat ggcggtcttc
     4261 ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt cggcgaggat
     4321 cgctttcact gggacgcgac cacgatcggc atttcgcttg ccgcatttgg cattctgcat
     4381 tcactcgccc aggcaatgat caccggccct gtagccgccc ggctcggcga aaggcgggca
     4441 ctcatgctcg gaatgattgc cgacggcaca ggctacatcc tgcttgcctt cgcgacacgg
     4501 ggatggatgg cgttcccgat catggtcctg cttgcttcgg gtggcatcgg aatgccggcg
     4561 ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc aggggcagct gcaaggctca
     4621 ctggcggcgc tcaccagcct gacctcgatc gtcggacccc tcctcttcac ggcgatctat
     4681 gcggcttcta taacaacgtg gaacgggtgg gcatggattg caggcgctgc cctctacttg
     4741 ctctgcctgc cggcgctgcg tcgcgggctt tggagcggcg cagggcaacg agccgatcgc
     4801 tgatcgtgga aacgataggc ctatgccatg cgggtcaagg cgacttccgg caagctatac
     4861 gcgccctagg agtgcggttg gaacgttggc ccagccagat actcccgatc acgagcagga
     4921 cgccgatgat ttgaagcgca ctcagcgtct gatccaagaa caaccatcct agcaacacgg
     4981 cggtccccgg gctgagaaag cccagtaagg aaacaactgt aggttcgagt cgcgagatcc
     5041 cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac gccaggccga
     5101 gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct actggaacga
     5161 gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc acgggaggtt
     5221 gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc aggcccgctg
     5281 cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag
     5341 ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc agagcggcag
     5401 agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca
     5461 ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt gcgccgagga
     5521 tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc acgagcaata
     5581 aacccgccgg caacgcccgc agcagcatac cggcgacccc tcggcctcgc tgttcgggct
     5641 ccacgaaaac gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc tcctgtttga
     5701 agaccgacag cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg gcatctcgca
     5761 accgttcagc gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg gacccgaaca
     5821 tctctggagc tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc acgtcggccg
     5881 ctccaagccg tcgaatctga gccttaatca caattgtcaa ttttaatcct ctgtttatcg
     5941 gcagttcgta gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc
     6001 agtgcccgct tgttcctgaa atgccagtaa agcgctggct gctgaacccc cagccggaac
     6061 tgaccccaca aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc
     6121 caccaggccg ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt
     6181 cacgcgggtg gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg
     6241 ctcccggtgc gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta
     6301 cttctcccat atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc
     6361 gatcaggacc tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag
     6421 cagcgacacc gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg
     6481 taggcgcgac aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag
     6541 gtcctggcaa agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc
     6601 gtactccaac acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt
     6661 gtaggtgatc ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg
     6721 cgggattttc ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc
     6781 tcgcatcgtg tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg
     6841 ctgcttcgtg tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc
     6901 ctcgccggcg gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga
     6961 cttcgccaaa cctgccgcct cctgttcgag acgacgcgaa cgctccacgg cggccgatgg
     7021 cgcgggcagg gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg
     7081 ctggaccatc gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct
     7141 tgcgatggtt tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc
     7201 cttccggtca aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg
     7261 ggcaatgtgc ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac
     7321 cttatcggca atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt
     7381 ccgaatcttg ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc
     7441 ggcctgagag ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc
     7501 atcgttgcgc cactcttcat taaccgctat atcgaaaatt gcttgcggct tgttagaatt
     7561 gccatgacgt acctcggtgt cacgggtaag attaccgata aactggaact gattatggct
     7621 catatcgaaa gtctccttga gaaaggagac tctagtttag ctaaacattg gttccgctgt
     7681 caagaacttt agcggctaaa attttgcggg ccgcgaccaa aggtgcgagg ggcggcttcc
     7741 gctgtgtaca accagatatt tttcaccaac atccttcgtc tgctcgatga gcggggcatg
     7801 acgaaacatg agctgtcgga gagggcaggg gtttcaattt cgtttttatc agacttaacc
     7861 aacggtaagg ccaacccctc gttgaaggtg atggaggcca ttgccgacgc cctggaaact
     7921 cccctacctc ttctcctgga gtccaccgac cttgaccgcg aggcactcgc ggagattgcg
     7981 ggtcatcctt tcaagagcag cgtgccgccc ggatacgaac gcatcagtgt ggttttgccg
     8041 tcacataagg cgtttatcgt aaagaaatgg ggcgacgaca cccgaaaaaa gctgcgtgga
     8101 aggctctgac gccaagggtt agggcttgca cttccttctt tagccgctaa aacggcccct
     8161 tctctgcggg ccgtcggctc gcgcatcata tcgacatcct caacggaagc cgtgccgcga
     8221 atggcatcgg gcgggtgcgc tttgacagtt gttttctatc agaaccccta cgtcgtgcgg
     8281 ttcgattagc tgtttgtctt gcaggctaaa cactttcggt atatcgtttg cctgtgcgat
     8341 aatgttgcta atgatttgtt gcgtaggggt tactgaaaag tgagcgggaa agaagagttt
     8401 cagaccatca aggagcgggc caagcgcaag ctggaacgcg acatgggtgc ggacctgttg
     8461 gccgcgctca acgacccgaa aaccgttgaa gtcatgctca acgcggacgg caaggtgtgg
     8521 cacgaacgcc ttggcgagcc gatgcggtac atctgcgaca tgcggcccag ccagtcgcag
     8581 gcgattatag aaacggtggc cggattccac ggcaaagagg tcacgcggca ttcgcccatc
     8641 ctggaaggcg agttcccctt ggatggcagc cgctttgccg gccaattgcc gccggtcgtg
     8701 gccgcgccaa cctttgcgat ccgcaagcgc gcggtcgcca tcttcacgct ggaacagtac
     8761 gtcgaggcgg gcatcatgac ccgcgagcaa tacgaggtca ttaaaagcgc cgtgattgat
     8821 gatatagcgg cccggctgct cctggttctc gcgcaccgaa atgggtgact tcaccccgcg
     8881 ctctttgatc gtggcaccga tttccgcgat gctctccggg gaaaagccgg ggttgtcggc
     8941 cgtccgcggc tgatgcggat cttcgtcgat caggtccagg tccagctcga tagggccgga
     9001 accgccctga gacgccgcag gagcgtccag gaggctcgac aggtcgccga tgctatccaa
     9061 ccccaggccg gacggctgcg ccgcgcctgc ggcttcctga gcggccgcag cggtgttttt
     9121 cttggtggtc ttggcttgag ccgcagtcat tgggaaatct ccatcttcgt gaacacgtaa
     9181 tcagccaggg cgcgaacctc tttcgatgcc ttgcgcgcgg ccgttttctt gatcttccag
     9241 accggcacac cggatgcgag ggcatcggcg atgctgctgc gcaggccaac ggtggccgga
     9301 atcatcatct tggggtacgc ggccagcagc tcggcttggt ggcgcgcgtg gcgcggattc
     9361 cgcgcatcga ccttgctggg caccatgcca aggaattgca gcttggcgtt cttctggcgc
     9421 acgttcgcaa tggtcgtgac catcttcttg atgccctgga tgctgtacgc ctcaagctcg
     9481 atgggggaca gcacatagtc ggccgcgaag agggcggccg ccaggccgac gccaagggtc
     9541 ggggccgtgt cgatcaggca cacgtcgaag ccttggttcg ccagggcctt gatgttcgcc
     9601 ccgaacagct cgcgggcgtc gtccagcgac agccgttcgg cgttcgccag taccgggttg
     9661 gactcgatga gggcgaggcg cgcggcctgg ccgtcgccgg ctgcgggtgc ggtttcggtc
     9721 cagccgccgg cagggacagc gccgaacagc ttgcttgcat gcaggccggt agcaaagtcc
     9781 ttgagcgtgt aggacgcatt gccctggggg tccaggtcga tcacggcaac ccgcaagccg
     9841 cgctcgaaaa agtcgaaggc aagatgcaca agggtcgaag tcttgccgac gccgcctttc
     9901 tggttggccg tgaccaaagt tttcatcgtt tggtttcctg ttttttcttg gcgtccgctt
     9961 cccacttccg gacgatgtac gcctgatgtt ccggcagaac cgccgttacc cgcgcgtacc
    10021 cctcgggcaa gttcttgtcc tcgaacgcgg cccacacgcg atgcaccgct tgcgacactg
    10081 cgcccctggt cagtcccagc gacgttgcga acgtcgcctg tggcttccca tcgactaaga
    10141 cgccccgcgc tatctcgatg gtctgctgcc ccacttccag cccctggatc gcctcctgga
    10201 actggctttc ggtaagccgt ttcttcatgg ataacaccca taatttgctc cgcgccttgg
    10261 ttgaacatag cggtgacagc cgccagcaca tgagagaagt ttagctaaac atttctcgca
    10321 cgtcaacacc tttagccgct aaaactcgtc cttggcgtaa caaaacaaaa gcccggaaac
    10381 cgggctttcg tctcttgccg cttatggctc tgcacccggc tccatcacca acaggtcgcg
    10441 cacgcgcttc actcggttgc ggatcgacac tgccagccca acaaagccgg ttgccgccgc
    10501 cgccaggatc gcgccgatga tgccggccac accggccatc gcccaccagg tcgccgccct
    10561 cactgcccgg cacctggtcg ctgaatgtcg atgccagcac ctgcggcacg tcaatgcttc
    10621 cgggcgtcgc gctcgggctg atcgcccatc ccgttactgc cccgatcccg gcaatggcaa
    10681 ggactgccag cg
//
