LOCUS       62056_18880       11940 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11940)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..712)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    complement(1289..1762)
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     primer_bind     complement(1811..1827)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1835..1851)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1859..1889)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1904..1925)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2160..2528)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(2561..2670)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(4032..4748)
                     /codon_start=1
                     /label=EYFP
                     /note="enhanced YFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             4854..6050
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(7327..8472)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga
       61 ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt cggcgtgcgc
      121 ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat tggtttaaaa
      181 gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt gcaaatgctg
      241 gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca tctgtcagca
      301 ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc gcccctcaag
      361 tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg gaaactcgcg
      421 taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc cggccgaaat
      481 cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca agtgtcaacg
      541 tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca acgccggcgg
      601 ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg gccatagacg
      661 gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc gctcttccgc
      721 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      781 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      841 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      901 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      961 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1021 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1081 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     1141 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     1201 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgacagg cattgatgga
     1261 atcgtagtct cacgctgata gtctgatcga caatacaagt gggaccgtgg tcccagaccg
     1321 ataatcagac cgacaacacg agtgggatcg tggtcccaga ctaataatca gaccgacgat
     1381 acgagtggga ccgtggtccc agactaataa tcagaccgac gatacgagtg ggaccgtggt
     1441 tccagactaa taatcagacc gacgatacga gtgggaccgt ggtcccagac taataatcag
     1501 accgacgata cgagtgggac catggtccca gactaataat cagaccgacg atacgagtgg
     1561 gaccgtggtc ccagtctgat tatcagaccg acgatacgag tgggaccgtg gtcccagact
     1621 aataatcaga ccgacgatac gagtgggacc gtggtcccag actaataatc agaccgacga
     1681 tacgagtggg accgtggtcc cagtctgatt atcagaccga cgatacaagt ggaacagtgg
     1741 gcccagagag aatattcagg ccggatcctc tagagtcgac ctgcaggcat gcaagcttgg
     1801 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     1861 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     1921 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     1981 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcggtcttgc
     2041 cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg atggagcgca
     2101 tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat
     2161 ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc ctgcttctct
     2221 tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg
     2281 tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt gatgcgggcc
     2341 agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg gccgacggcc
     2401 agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat cgctcttcgt
     2461 tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg cttggtttca
     2521 tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca
     2581 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc
     2641 gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag
     2701 tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa
     2761 aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca cgcttcccga
     2821 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2881 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2941 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3001 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3061 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3121 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcca
     3181 gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc agggcatgaa
     3241 aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag gggatgttgt
     3301 ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga tcaatcgtca
     3361 ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa tccatcgaca
     3421 atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa ggcgtctatc
     3481 gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg ctcgccggca
     3541 tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct gattgtcatc
     3601 agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg aagctgcgcg
     3661 tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg cgcgccatcg
     3721 cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa tgagcgccag
     3781 tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc ttcggccagt
     3841 gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg ctgaaccccc
     3901 aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt caacaggtcc
     3961 agggcggcac ggatcactgt attcggctgc aactttgtca tgcttccccc tcctccactc
     4021 cctccgccgc ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
     4081 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactggtag
     4141 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
     4201 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
     4261 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
     4321 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
     4381 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
     4441 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
     4501 tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact gcaggccgta gccgaaggtg
     4561 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
     4621 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
     4681 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
     4741 ctcaccatgc ttgacacttt atcactgata aacataatat gtccaccaac ttatcagtga
     4801 taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca gacatgaaac
     4861 ccaacatacc cctgatcgta attctgagca ctgtcgcgct cgacgctgtc ggcatcggcc
     4921 tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac gacgtcaccg
     4981 cccactatgg cattctgctg gcgctgtatg cgttggtgca atttgcctgc gcacctgtgc
     5041 tgggcgcgct gtcggatcgt ttcgggcggc ggccaatctt gctcgtctcg ctggccggcg
     5101 ccactgtcga ctacgccatc atggcgacag cgcctttcct ttgggttctc tatatcgggc
     5161 ggatcgtggc cggcatcacc ggggcgactg gggcggtagc cggcgcttat attgccgata
     5221 tcactgatgg cgatgagcgc gcgcggcact tcggcttcat gagcgcctgt ttcgggttcg
     5281 ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg tttctccccc cacgctccgt
     5341 tcttcgccgc ggcagccttg aacggcctca atttcctgac gggctgtttc cttttgccgg
     5401 agtcgcacaa aggcgaacgc cggccgttac gccgggaggc tctcaacccg ctcgcttcgt
     5461 tccggtgggc ccggggcatg accgtcgtcg ccgccctgat ggcggtcttc ttcatcatgc
     5521 aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt cggcgaggat cgctttcact
     5581 gggacgcgac cacgatcggc atttcgcttg ccgcatttgg cattctgcat tcactcgccc
     5641 aggcaatgat caccggccct gtagccgccc ggctcggcga aaggcgggca ctcatgctcg
     5701 gaatgattgc cgacggcaca ggctacatcc tgcttgcctt cgcgacacgg ggatggatgg
     5761 cgttcccgat catggtcctg cttgcttcgg gtggcatcgg aatgccggcg ctgcaagcaa
     5821 tgttgtccag gcaggtggat gaggaacgtc aggggcagct gcaaggctca ctggcggcgc
     5881 tcaccagcct gacctcgatc gtcggacccc tcctcttcac ggcgatctat gcggcttcta
     5941 taacaacgtg gaacgggtgg gcatggattg caggcgctgc cctctacttg ctctgcctgc
     6001 cggcgctgcg tcgcgggctt tggagcggcg cagggcaacg agccgatcgc tgatcgtgga
     6061 aacgataggc ctatgccatg cgggtcaagg cgacttccgg caagctatac gcgccctagg
     6121 agtgcggttg gaacgttggc ccagccagat actcccgatc acgagcagga cgccgatgat
     6181 ttgaagcgca ctcagcgtct gatccaagaa caaccatcct agcaacacgg cggtccccgg
     6241 gctgagaaag cccagtaagg aaacaactgt aggttcgagt cgcgagatcc cccggaacca
     6301 aaggaagtag gttaaacccg ctccgatcag gccgagccac gccaggccga gaacattggt
     6361 tcctgtaggc atcgggattg gcggatcaaa cactaaagct actggaacga gcagaagtcc
     6421 tccggccgcc agttgccagg cggtaaaggt gagcagaggc acgggaggtt gccacttgcg
     6481 ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc aggcccgctg cgacgccgac
     6541 aggatctagc gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag ttccgcaaat
     6601 agcccccagg accgccatca atcgtatcgg gctacctagc agagcggcag agatgaacac
     6661 gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc cgcccggcag gcggtagacc
     6721 gaaataaaca acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc
     6781 atccaccaga ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc
     6841 aacgcccgca gcagcatacc ggcgacccct cggcctcgct gttcgggctc cacgaaaacg
     6901 ccggacagat gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa gaccgacagc
     6961 ccaatgatct cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg
     7021 aacgcctcca tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct
     7081 ttcttcaggg ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt
     7141 cgaatctgag ccttaatcac aattgtcaat tttaatcctc tgtttatcgg cagttcgtag
     7201 agcgcgccgt gcgcccgagc gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg
     7261 ttcctgaaat gccagtaaag cgctggctgc tgaaccccca gccggaactg accccacaag
     7321 gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct
     7381 gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga
     7441 atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga
     7501 gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat
     7561 gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg
     7621 gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga
     7681 ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag
     7741 gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag
     7801 ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac
     7861 ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt
     7921 cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt
     7981 gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc
     8041 cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg
     8101 tttcagcaac gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt
     8161 ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc
     8221 tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc
     8281 agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga
     8341 gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc
     8401 ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa
     8461 cgtccgattc attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc
     8521 ttattcctga tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat
     8581 gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc
     8641 ctgcacgaat accagcgacc ccttgcccaa atacttgccg tgggcctcgg cctgagagcc
     8701 aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca
     8761 ctcttcatta accgctatat cgaaaattgc ttgcggcttg ttagaattgc catgacgtac
     8821 ctcggtgtca cgggtaagat taccgataaa ctggaactga ttatggctca tatcgaaagt
     8881 ctccttgaga aaggagactc tagtttagct aaacattggt tccgctgtca agaactttag
     8941 cggctaaaat tttgcgggcc gcgaccaaag gtgcgagggg cggcttccgc tgtgtacaac
     9001 cagatatttt tcaccaacat ccttcgtctg ctcgatgagc ggggcatgac gaaacatgag
     9061 ctgtcggaga gggcaggggt ttcaatttcg tttttatcag acttaaccaa cggtaaggcc
     9121 aacccctcgt tgaaggtgat ggaggccatt gccgacgccc tggaaactcc cctacctctt
     9181 ctcctggagt ccaccgacct tgaccgcgag gcactcgcgg agattgcggg tcatcctttc
     9241 aagagcagcg tgccgcccgg atacgaacgc atcagtgtgg ttttgccgtc acataaggcg
     9301 tttatcgtaa agaaatgggg cgacgacacc cgaaaaaagc tgcgtggaag gctctgacgc
     9361 caagggttag ggcttgcact tccttcttta gccgctaaaa cggccccttc tctgcgggcc
     9421 gtcggctcgc gcatcatatc gacatcctca acggaagccg tgccgcgaat ggcatcgggc
     9481 gggtgcgctt tgacagttgt tttctatcag aacccctacg tcgtgcggtt cgattagctg
     9541 tttgtcttgc aggctaaaca ctttcggtat atcgtttgcc tgtgcgataa tgttgctaat
     9601 gatttgttgc gtaggggtta ctgaaaagtg agcgggaaag aagagtttca gaccatcaag
     9661 gagcgggcca agcgcaagct ggaacgcgac atgggtgcgg acctgttggc cgcgctcaac
     9721 gacccgaaaa ccgttgaagt catgctcaac gcggacggca aggtgtggca cgaacgcctt
     9781 ggcgagccga tgcggtacat ctgcgacatg cggcccagcc agtcgcaggc gattatagaa
     9841 acggtggccg gattccacgg caaagaggtc acgcggcatt cgcccatcct ggaaggcgag
     9901 ttccccttgg atggcagccg ctttgccggc caattgccgc cggtcgtggc cgcgccaacc
     9961 tttgcgatcc gcaagcgcgc ggtcgccatc ttcacgctgg aacagtacgt cgaggcgggc
    10021 atcatgaccc gcgagcaata cgaggtcatt aaaagcgccg tgattgatga tatagcggcc
    10081 cggctgctcc tggttctcgc gcaccgaaat gggtgacttc accccgcgct ctttgatcgt
    10141 ggcaccgatt tccgcgatgc tctccgggga aaagccgggg ttgtcggccg tccgcggctg
    10201 atgcggatct tcgtcgatca ggtccaggtc cagctcgata gggccggaac cgccctgaga
    10261 cgccgcagga gcgtccagga ggctcgacag gtcgccgatg ctatccaacc ccaggccgga
    10321 cggctgcgcc gcgcctgcgg cttcctgagc ggccgcagcg gtgtttttct tggtggtctt
    10381 ggcttgagcc gcagtcattg ggaaatctcc atcttcgtga acacgtaatc agccagggcg
    10441 cgaacctctt tcgatgcctt gcgcgcggcc gttttcttga tcttccagac cggcacaccg
    10501 gatgcgaggg catcggcgat gctgctgcgc aggccaacgg tggccggaat catcatcttg
    10561 gggtacgcgg ccagcagctc ggcttggtgg cgcgcgtggc gcggattccg cgcatcgacc
    10621 ttgctgggca ccatgccaag gaattgcagc ttggcgttct tctggcgcac gttcgcaatg
    10681 gtcgtgacca tcttcttgat gccctggatg ctgtacgcct caagctcgat gggggacagc
    10741 acatagtcgg ccgcgaagag ggcggccgcc aggccgacgc caagggtcgg ggccgtgtcg
    10801 atcaggcaca cgtcgaagcc ttggttcgcc agggccttga tgttcgcccc gaacagctcg
    10861 cgggcgtcgt ccagcgacag ccgttcggcg ttcgccagta ccgggttgga ctcgatgagg
    10921 gcgaggcgcg cggcctggcc gtcgccggct gcgggtgcgg tttcggtcca gccgccggca
    10981 gggacagcgc cgaacagctt gcttgcatgc aggccggtag caaagtcctt gagcgtgtag
    11041 gacgcattgc cctgggggtc caggtcgatc acggcaaccc gcaagccgcg ctcgaaaaag
    11101 tcgaaggcaa gatgcacaag ggtcgaagtc ttgccgacgc cgcctttctg gttggccgtg
    11161 accaaagttt tcatcgtttg gtttcctgtt ttttcttggc gtccgcttcc cacttccgga
    11221 cgatgtacgc ctgatgttcc ggcagaaccg ccgttacccg cgcgtacccc tcgggcaagt
    11281 tcttgtcctc gaacgcggcc cacacgcgat gcaccgcttg cgacactgcg cccctggtca
    11341 gtcccagcga cgttgcgaac gtcgcctgtg gcttcccatc gactaagacg ccccgcgcta
    11401 tctcgatggt ctgctgcccc acttccagcc cctggatcgc ctcctggaac tggctttcgg
    11461 taagccgttt cttcatggat aacacccata atttgctccg cgccttggtt gaacatagcg
    11521 gtgacagccg ccagcacatg agagaagttt agctaaacat ttctcgcacg tcaacacctt
    11581 tagccgctaa aactcgtcct tggcgtaaca aaacaaaagc ccggaaaccg ggctttcgtc
    11641 tcttgccgct tatggctctg cacccggctc catcaccaac aggtcgcgca cgcgcttcac
    11701 tcggttgcgg atcgacactg ccagcccaac aaagccggtt gccgccgccg ccaggatcgc
    11761 gccgatgatg ccggccacac cggccatcgc ccaccaggtc gccgccctca ctgcccggca
    11821 cctggtcgct gaatgtcgat gccagcacct gcggcacgtc aatgcttccg ggcgtcgcgc
    11881 tcgggctgat cgcccatccc gttactgccc cgatcccggc aatggcaagg actgccagcg
//
