LOCUS       40924_37113        3053 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRJK046, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Krom RJ, Bhargava P, Lobritz MA, Collins JJ.
  TITLE     Engineered Phagemids for Nonlytic, Targeted Antibacterial Therapies
  JOURNAL   Nano Lett. 15 (7), 4808-4813 (2015)
  PUBMED    26044909
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Krom RJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) Molecular Medicine, Boston University, 72 
            East Concord St, Boston, MA 02118, USA
REFERENCE   3  (bases 1 to 3053)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3053)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nano 
            Lett."; date: "2015"; volume: "15"; issue: "7"; pages: "4808-4813"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-JUN-2015) Molecular Medicine, Boston University, 72 East Concord
            St, Boston, MA 02118, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        103..176
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     RBS             202..210
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             217..276
                     /codon_start=1
                     /product="apidaecin"
                     /label=apidaecin
                     /protein_id="AKQ98638.1"
                     /translation="MGNNRPVYIPQPRPPHPRI"
     RBS             315..323
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             329..388
                     /codon_start=1
                     /product="apidaecin"
                     /label=apidaecin
                     /protein_id="AKQ98639.1"
                     /translation="MGNNRPVYIPQPRPPHPRI"
     terminator      409..503
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      620..1047
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      1072..1158
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1322..1910)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1998..2092)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2126..2917)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 gacgtctgtg caagtactac tgttctgcag tcacttgaat tcgataccca gctgggtgga
       61 gtgcaccaag gagcatgcga aggaaacgtt tcgcagaagc tttccctatc agtgatagag
      121 attgacatcc ctatcagtga tagagatact gagcacatca gcaggacgca ctgaccggta
      181 cccaacaggg attcgttaat ttaaggaggt ctccaaatgg gcaacaaccg cccggtgtat
      241 attccgcagc cgcgcccgcc gcatccgcgc atttaaccgc ggagtatttg tacagaggta
      301 caactcaact aatataagga ggttcaaaat gggcaacaac cgcccggtgt atattccgca
      361 gccgcgcccg ccgcatccgc gcatttaagg atcccgagaa ttggcttgga ctcctgttga
      421 tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc
      481 gggcgttttt tattggtgag aatccaagca gtagtcagag ctcggactgc ttaagtcgct
      541 ccatatgctc gttcccggga ctacacaatt gtcccccggc gccagggttg atatctatcg
      601 ccctagggac cgtctcgaga gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      661 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      721 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      781 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
      841 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
      901 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
      961 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     1021 tgatttaaca aaaatttaac gcgaattaaa tattaatcca acgcgtggca tcaaataaaa
     1081 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1141 ctcctgagta ggacaaatcc gccgccctag acttaggcgt tcggctgcgg cgagcggtat
     1201 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1261 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1321 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1381 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1441 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1501 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     1561 ccaagctggg ctgtctgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     1621 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     1681 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     1741 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     1801 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     1861 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     1921 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     1981 tcatggctag tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg
     2041 aacaaatcca gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagccaa
     2101 ttctcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc
     2161 gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc
     2221 caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac
     2281 ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca
     2341 agcaggcatc gccgtgggtc acgacgagat cctcgccgtc gggcatacgc gccttgagcc
     2401 tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga
     2461 caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga
     2521 atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata
     2581 ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata
     2641 gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agccgcgcaa ggaacgcccg
     2701 tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca
     2761 ggtcggtctt gacaaaaaga accgggcgac cctgcgctga cagccggaac acggcggcat
     2821 cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagccg
     2881 ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct catcctgtct
     2941 cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt
     3001 ttactttgca gggcttccca accttaccag agggcggccc aactggcaat tcc
//
