LOCUS       40924_37108        3172 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRJK037, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Krom RJ, Bhargava P, Lobritz MA, Collins JJ.
  TITLE     Engineered Phagemids for Nonlytic, Targeted Antibacterial Therapies
  JOURNAL   Nano Lett. 15 (7), 4808-4813 (2015)
  PUBMED    26044909
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Krom RJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JUN-2015) Molecular Medicine, Boston University, 72 
            East Concord St, Boston, MA 02118, USA
REFERENCE   3  (bases 1 to 3172)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3172)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nano 
            Lett."; date: "2015"; volume: "15"; issue: "7"; pages: "4808-4813"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-JUN-2015) Molecular Medicine, Boston University, 72 East Concord
            St, Boston, MA 02118, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        103..176
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator
                     sites (Lutz and Bujard, 1997)"
     CDS             214..336
                     /codon_start=1
                     /product="cecropin PR-39"
                     /label=cecropin PR-39
                     /protein_id="AKQ98636.1"
                     /translation="MRRRPRPPYLPRPRPPPFFPPRLPPRIPPGFPPRFPPRFP"
     CDS             385..507
                     /codon_start=1
                     /product="cecropia PR-39"
                     /label=cecropia PR-39
                     /protein_id="AKQ98637.1"
                     /translation="MRRRPRPPYLPRPRPPPFFPPRLPPRIPPGFPPRFPPRFP"
     terminator      528..622
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      739..1166
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      1191..1277
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(1441..2029)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(2117..2211)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2245..3036)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 gacgtctgtg caagtactac tgttctgcag tcacttgaat tcgataccca gctgggtgga
       61 gtgcaccaag gagcatgcga aggaaacgtt tcgcagaagc tttccctatc agtgatagag
      121 attgacatcc ctatcagtga tagagatact gagcacatca gcaggacgca ctgaccggta
      181 ccctataagt tacacaacat aaggaggaat ataatgcgtc gtcgtccgcg tccgccgtac
      241 ctgccgcgtc cgcgtccgcc gccgttcttc ccgccgcgtc tgccgccgcg tatcccgccg
      301 ggtttcccgc cgcgtttccc gccgcgtttc ccgtaaccgc ggagtatttg tacattctat
      361 caaagtatca taaggaggag aataatgcgt cgtcgtccgc gtccgccgta cctgccgcgt
      421 ccgcgtccgc cgccgttctt cccgccgcgt ctgccgccgc gtatcccgcc gggtttcccg
      481 ccgcgtttcc cgccgcgttt cccgtaagga tcccgagaat tggcttggac tcctgttgat
      541 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg
      601 ggcgtttttt attggtgaga atccaagcag tagtcagagc tcggactgct taagtcgctc
      661 catatgctcg ttcccgggac tacacaattg tcccccggcg ccagggttga tatctatcgc
      721 cctagggacc gtctcgagag cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      781 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      841 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      901 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      961 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     1021 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     1081 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     1141 gatttaacaa aaatttaacg cgaattaaat attaatccaa cgcgtggcat caaataaaac
     1201 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1261 tcctgagtag gacaaatccg ccgccctaga cttaggcgtt cggctgcggc gagcggtatc
     1321 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1381 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1441 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1501 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1561 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1621 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1681 caagctgggc tgtctgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1741 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1801 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1861 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     1921 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1981 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2041 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2101 catggctagt gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga
     2161 acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt ccaagccaat
     2221 tctcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     2281 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     2341 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     2401 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     2461 gcaggcatcg ccgtgggtca cgacgagatc ctcgccgtcg ggcatacgcg ccttgagcct
     2521 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     2581 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     2641 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     2701 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
     2761 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gccgcgcaag gaacgcccgt
     2821 cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt tcattcaggg caccggacag
     2881 gtcggtcttg acaaaaagaa ccgggcgacc ctgcgctgac agccggaaca cggcggcatc
     2941 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagccgc
     3001 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     3061 ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt
     3121 tactttgcag ggcttcccaa ccttaccaga gggcggccca actggcaatt cc
//
