LOCUS       40924_37078        5241 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRIE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Welch MD, Reed SC, Lamason RL, Serio AW.
  TITLE     Expression of an Epitope-Tagged Virulence Protein in Rickettsia 
            parkeri Using Transposon Insertion
  JOURNAL   PLoS ONE 7 (5), E37310 (2012)
  PUBMED    22624012
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Reed SCO., Welch MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2012) Molecular and Cell Biology, University of 
            California, Berkeley, 305 Life Sciences Addition, Berkeley, CA 
            94720-3200, USA
REFERENCE   3  (bases 1 to 5241)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5241)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2012"; volume: "7"; issue: "5"; pages: "E37310"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-FEB-2012) Molecular and Cell Biology, University of California, 
            Berkeley, 305 Life Sciences Addition, Berkeley, CA 94720-3200, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    112..133
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        148..178
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    186..202
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     210..226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      495..671
                     /label=flgBp
                     /note="flgBp"
                     /regulatory_class="promoter"
     CDS             672..1718
                     /codon_start=1
                     /product="Himar1"
                     /label=Himar1
                     /note="Mariner transposase"
                     /protein_id="AFJ44823.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQQRVDDSERCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIRELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYRNGIKKLEGRYNRCI
                     ALEGNYVE"
     CDS             2092..2898
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(2986..3002)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3143..3178
                     /label=ITR1
                     /note="ITR1"
     rep_origin      complement(3279..3867)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4076..4175
                     /label=MCS2
                     /note="MCS2"
     misc_feature    complement(4408..4431)
                     /label=MCS1
                     /note="MCS1"
     regulatory      complement(4432..4572)
                     /label=OmpAp
                     /note="OmpAp"
                     /regulatory_class="promoter"
     CDS             complement(4591..5043)
                     /codon_start=1
                     /product="Rparr-2"
                     /label=Rparr-2
                     /note="rifampicin resistance protein"
                     /protein_id="AFJ44822.1"
                     /translation="MVKDWIPISHDNYKQVQGPFYHGTKANLAIGDLLTTGFISHFEDG
                     RILKHIYFSALMEPAVWGAELAMSLSGLEGRGYIYIVEPTGPFEDDPNLTNKKFPGNPT
                     QSYRTCEPLRIVGVVEDWEGHPVELIRGMLDSLEDLKRRGLHVIED"
     regulatory      complement(5044..5204)
                     /label=rpsLp
                     /note="rpsLp"
                     /regulatory_class="promoter"
ORIGIN
        1 gcggccgcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
       61 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      121 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      181 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt
      241 cgagaattcg cccttacgcg ttaatacccg agcttcaagg aagatttcct attaaggttg
      301 aacttaagag cttaagcata gacgatttga aaaaaatttt aaaacaaaca aaaaattctt
      361 taataaaaca atatgttgcg atgtttaagg tttatgattt agatttaaag tttagtgagg
      421 aagctataga tagaattgca gagcttactt ttaatatgaa tcttgagagt gaaaatcttg
      481 gtgccagaag acttcacggt gttatggaaa tagtgcttgc agatcttttt tttgaagtgc
      541 ctggcagtaa gttgaaaaaa tttgaaataa acttggacta tgttaataaa aaaatacaaa
      601 ttaacgaaca aaaagatttg aattattata taatttagtt aaaagcaatt ttaaatgagg
      661 gaggtttcca tatggaaaaa aaggaatttc gtgttttgat aaaatactgt tttctgaagg
      721 gaaaaaatac agtggaagca aaaacttggc ttgataatga gtttccggac tctgccccag
      781 ggaaatcaac aataattgat tggtatgcaa aattcaagcg tggtgaaatg agcacggagg
      841 acggtgaacg cagcggacgc ccgaaagagg tggttaccga cgaaaacatc aaaaaaatcc
      901 acaaaatgat tttgaatgac cgtaaaatga agttgatcga gatagcagag gccttaaaga
      961 tatcgaagga acgtgttggt catatcattc atcaatattt ggatatgcgg aagctctgtg
     1021 caaaatgggt gccgcgcgag ctcacatttg accaaaaaca acaacgtgtt gatgattctg
     1081 agcggtgttt gcagctgtta actcgcaata cacccgagtt tttccgtcga tatgtgacaa
     1141 tggatgaaac atggctccat cactacactc ctgagtccaa tcgacagtcg gctgagtgga
     1201 cggcgaccgg tgaaccgtct ccgaagcgtg gaaagactca aaagtccgct ggcaaagtaa
     1261 tggcctctgt tttttgggat gcgcatggaa taatttttat cgattatctt gagaagggaa
     1321 aaaccatcaa cagtgactat tatatggcgt tattggagcg tttgaaggtc gaaatcgcgg
     1381 caaaacggcc ccacatgaag aagaaaaaag tgttgttcca ccaagacaac gcaccgtgcc
     1441 acaagtcatt gagaacgatg gcaaaaattc gtgaattggg cttcgaattg cttccccacc
     1501 cgccgtattc tccagatctg gcccccagcg actttttctt gttctcagac ctcaaaagga
     1561 tgctcgcagg gaaaaaattt ggctgcaatg aagaggtgat cgccgaaact gaggcctatt
     1621 ttgaggcaaa accgaaggag tactaccgaa atggtatcaa aaaattggaa ggtcgttata
     1681 atcgttgtat cgctcttgaa gggaactatg ttgaataaag atcttgtctg tcgcctcttg
     1741 tggcttccgg aggagttatt tataataaaa taaagaatta cgacttgaaa atttttaatc
     1801 aaaaaaataa agttgaagat tataaaaaga agtacgaaga tagagagaga aaagtggaag
     1861 gtgaacttaa taccttggat tttaccgtta agcgcatgaa agatcaagaa aatacattaa
     1921 aggcttttga ttttaatcaa agaaataaat aataataatt atttttaatg ctattgctat
     1981 ttgcgtttct ttttttttaa tttttgtgct attcttttta acaggcaaaa ggatttgcca
     2041 aagtcagaaa tttaaatttt atcatggagg aatgatatat gattatcaat catgagccat
     2101 attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg
     2161 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg
     2221 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
     2281 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
     2341 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca
     2401 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
     2461 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
     2521 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
     2581 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
     2641 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
     2701 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
     2761 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
     2821 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
     2881 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt
     2941 acgctgactt gacgggacgg cagatcttca ttcgccagat ctggcactgg ccgtcgtttt
     3001 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3061 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3121 gcgcagcctg aatggcgaat ggcggccgct aacaggttgg ctgataagtc cccggtctcc
     3181 taaggatgaa cttgccgggg atctagtgca ggaaagaaca tgtgagcaaa aggccagcaa
     3241 aagcccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     3301 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     3361 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     3421 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     3481 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     3541 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     3601 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     3661 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     3721 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     3781 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     3841 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3901 gctcagtgga acgaaaactc acgttaaggg attttggacg cgtaagccga tctcggcttg
     3961 aacgaattgt taggtggcgg tacttgggtc gatatcaaag tgcatcactt cttcccgtat
     4021 gcccaacttt gtatagagag ccactgcggg atcgtcaccg taatctgctt gcacggacgt
     4081 cggtaccggc gcgccggatc ccccgggcgt acgtgatcag gcgccctgca ggccggccac
     4141 cggtggctag cgagctcccg cggctcgagg tcgacactag ttaaatgtca ttcccgcttt
     4201 gcgggaatga cataaaacac catataaatc taccctttaa acttcttagt tatttcaact
     4261 attcttttac caaagaaatt aatgcatgca tgcaagctgg catatgtcgc aatgacgctt
     4321 ctcaaaacta cattataaag caaaaaagcc tatatagtca aactatatag gcttttttta
     4381 tgattataga acggttgctt attagcctct agacttaaga atattaagct tatgtaaaac
     4441 cttaatcaaa attaaatcac ttaaaaagtg ttataacgca acttttttaa tatgtctatt
     4501 ataattaata acatagatga tatttttatg tatagattat atgtcattcc gtagcggtgt
     4561 tggccttgtt gccatatgat gcatcttaag ttaatcttca ataacatgta aaccacgacg
     4621 ttttaaatct tctaatgaat ctaacattcc tcttattaat tcaacaggat gtccttccca
     4681 atcttcaaca acaccaacaa ttcttaaagg ttcacaagtt ctataagatt gtgttggatt
     4741 accaggaaat tttttatttg taagatttgg atcatcttca aatggtcctg ttggttcaac
     4801 tatatatata taaccacgac cttcaagacc agatagtgac atagcaagtt cagctcccca
     4861 aactgctggt tccattaaag ctgaaaaata aatatgttta agaattcgac catcttcaaa
     4921 atgagaaata aatcctgtag ttagtaaatc accaattgct aaattagctt tagttccatg
     4981 ataaaatggt ccttgtactt gtttataatt atcatgagaa ataggaatcc aatcttttac
     5041 catatgaaaa cactccaaaa aacttactta tatcactgat taaagggtaa aaatatactt
     5101 cacccttgac tctctttaaa tcatatctaa atacaaatta taagaacttg taaattacag
     5161 taaacttaaa ttataattat ttttcctata aaagcaagta tgttcctagg gcagaccggg
     5221 gacttatcag ccaacctgtt a
//
