LOCUS       40924_37068        8808 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRI857-BAC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Zhang K, Su H, Yang M, Ge J, Li G, Yi J, Wang Y.
  TITLE     Construction of a thermo-sensitive pRI857 vector for efficient DNA 
            capturing in Escherichia coli
  JOURNAL   Biotechnol. Lett. (2017) In press
  PUBMED    28251389
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   Zhang K, Wang Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-AUG-2016) College of Environmental and Biological 
            Engineering, Nanjing University of Science and Technology, 200 
            Xiaolingwei, Nanjing, Jiangsu 210094, China
REFERENCE   3  (bases 1 to 8808)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8808)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Lett. (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-AUG-2016) College of Environmental and Biological Engineering, 
            Nanjing University of Science and Technology, 200 Xiaolingwei, 
            Nanjing, Jiangsu 210094, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(4..795)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     RBS             799..807
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    950..971
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        986..1016
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1024..1040
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1060..1770
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
     regulatory      2026..2062
                     /regulatory_class="terminator"
     CDS             complement(2129..2761)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(2762..2864)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2980..3327
                     /codon_start=1
                     /gene="redF"
                     /product="RedF"
                     /label=redF
                     /protein_id="AQX17847.1"
                     /translation="MERRNRRTGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVP
                     VTPHTFRHSYAMHMLYAGIPLKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPE
                     SDAVAMLKQLS"
     gene            2980..3327
                     /gene="redF"
                     /label=redF
     rep_origin      3723..4337
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      4413..4632
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             4723..5475
                     /label=repE
                     /note="replication initiation protein for the bacterial F 
                     plasmid"
     misc_feature    5481..5731
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             6057..7229
                     /label=sopA
                     /note="partitioning protein for the bacterial F plasmid"
     CDS             7232..8200
                     /label=sopB
                     /note="partitioning protein for the bacterial F plasmid"
     misc_feature    8276..8749
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat
       61 accgtaaagc acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg
      121 ggtagccaac gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa
      181 tccagaaaag cggccatttt ccaccatgat attcggcaag caggcatcgc catgggtcac
      241 gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg gcgaacagtt cggctggcgc
      301 gagcccctga tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt
      361 acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag
      421 cgtatgcagc cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg
      481 agatgacagg agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc
      541 agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg
      601 cgctgcctcg tcctgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac
      661 cgggcgcccc tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg
      721 tgcccagtca tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc
      781 atcttgttca atcatacaac ctccttagta catgcaacca ttatcaccgc cagaggtaaa
      841 atagtcaaca cgcacggtgt tagatattta tcccttgcgg tgatagattt aacgtcagct
      901 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      961 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     1021 gaattgtgag cggataacaa tttcacacag gaaacagcta tgagcacaaa aaagaaacca
     1081 ttaacacaag agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa
     1141 aatgaacttg gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc
     1201 gttggtgctt tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttaca
     1261 aaaattctca aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag
     1321 atgtatgaag cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt
     1381 tctcatgttc aggcagggat gttctcacct aagcttagaa cctttaccaa aggtgatgcg
     1441 gagagatggg taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa
     1501 ggtaattcca tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt
     1561 ctcgttgacc ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt
     1621 gatgagttta ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta
     1681 aacccacagt acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc
     1741 gctagtcagt ggcctgaaga gacgtttggc tgatcggcaa ggtgttctgg ttaactgatg
     1801 aactatggga ccaaataaaa tatattgaag aaattaattg attacagaaa ctccttaaaa
     1861 ggagtttttt agttacctta attgaaataa acgaaataaa aactcgcaat taagcgagtt
     1921 ggaatttaaa tatgatatct acattatcag cagtaacatc aacctttgat acaaggttgt
     1981 tgcatttacc ttgattgaga tgttaattgt gttggcaatt atcagaaaaa agaacccgag
     2041 tgggttcttt tttatttttt aatctagcta gaactagtgg atcccccggg ctgcaggaat
     2101 tcgatatcaa gcttatcgat accgtcgacc actgttgtaa ttcattaagc attctgccga
     2161 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     2221 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     2281 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat
     2341 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     2401 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     2461 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     2521 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     2581 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     2641 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2701 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2761 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2821 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc
     2881 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
     2941 agtgatcttc cgtcacaggt atttattcgc gataagctca tggagcggcg taaccgtcgc
     3001 acaggaagga cagagaaagc gcggatctgg gaagtgacgg acagaacggt caggacctgg
     3061 attggggagg cggttgccgc cgctgctgct gacggtgtga cgttctctgt tccggtcaca
     3121 ccacatacgt tccgccattc ctatgcgatg cacatgctgt atgccggtat accgctgaaa
     3181 gttctgcaaa gcctgatggg acataagtcc atcagttcaa cggaagtcta cacgaaggtt
     3241 tttgcgctgg atgtggctgc ccggcaccgg gtgcagtttg cgatgccgga gtctgatgcg
     3301 gttgcgatgc tgaaacaatt atcctgagaa taaatgcctt ggcctttata tggaaatgtg
     3361 gaactgagtg gatatgctgt ttttgtctgt taaacagaga agctggctgt tatccactga
     3421 gaagcgaacg aaacagtcgg gaaaatctcc cattatcgta gagatccgca ttattaatct
     3481 caggagcctg tgtagcgttt ataggaagta gtgttctgtc atgatgcctg caagcggtaa
     3541 cgaaaacgat ttgaatatgc cttcaggaac aatagaaatc ttcgtgcggt gttacgttga
     3601 agtggagcgg attatgtcag caatggacag aacaacctaa tgaacacaga accatgatgt
     3661 ggtctgtcct tttacagcca gtagtgctcg ccgcagtcga gcgacagggc gaagccctcg
     3721 agctggttgc cctcgccgct gggctggcgg ccgtctatgg ccctgcaaac gcgccagaaa
     3781 cgccgtcgaa gccgtgtgcg agacaccgcg gccggccgcc ggcgttgtgg atacctcgcg
     3841 gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca
     3901 cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg
     3961 ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg
     4021 acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga
     4081 tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta
     4141 ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg
     4201 tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt
     4261 gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc
     4321 ccttctcgaa ccctcccggt cgagtgagcg aggaagcacc agggaacagc acttatatat
     4381 tctgcttaca cacgatgcct gaaaaaactt cccttggggt tatccactta tccacgggga
     4441 tatttttata attatttttt ttatagtttt tagatcttct tttttagagc gccttgtagg
     4501 cctttatcca tgctggttct agagaaggtg ttgtgacaaa ttgccctttc agtgtgacaa
     4561 atcaccctca aatgacagtc ctgtctgtga caaattgccc ttaaccctgt gacaaattgc
     4621 cctcagaaga agctgttttt tcacaaagtt atccctgctt attgactctt ttttatttag
     4681 tgtgacaatc taaaaacttg tcacacttca catggatctg tcatggcgga aacagcggtt
     4741 atcaatcaca agaaacgtaa aaatagcccg cgaatcgtcc agtcaaacga cctcactgag
     4801 gcggcatata gtctctcccg ggatcaaaaa cgtatgctgt atctgttcgt tgaccagatc
     4861 agaaaatctg atggcaccct acaggaacat gacggtatct gcgagatcca tgttgctaaa
     4921 tatgctgaaa tattcggatt gacctctgcg gaagccagta aggatatacg gcaggcattg
     4981 aagagtttcg cggggaagga agtggttttt tatcgccctg aagaggatgc cggcgatgaa
     5041 aaaggctatg aatcttttcc ttggtttatc aaacgtgcgc acagtccatc cagagggctt
     5101 tacagtgtac atatcaaccc atatctcatt cccttcttta tcgggttaca gaaccggttt
     5161 acgcagtttc ggcttagtga aacaaaagaa atcaccaatc cgtatgccat gcgtttatac
     5221 gaatccctgt gtcagtatcg taagccggat ggctcaggca tcgtctctct gaaaatcgac
     5281 tggatcatag agcgttacca gctgcctcaa agttaccagc gtatgcctga cttccgccgc
     5341 cgcttcctgc aggtctgtgt taatgagatc aacagcagaa ctccaatgcg cctctcatac
     5401 attgagaaaa agaaaggccg ccagacgact catatcgtat tttccttccg cgatatcact
     5461 tccatgacga caggatagtc tgagggttat ctgtcacaga tttgagggtg gttcgtcaca
     5521 tttgttctga cctactgagg gtaatttgtc acagttttgc tgtttccttc agcctgcatg
     5581 gattttctca tactttttga actgtaattt ttaaggaagc caaatttgag ggcagtttgt
     5641 cacagttgat ttccttctct ttcccttcgt catgtgacct gatatcgggg gttagttcgt
     5701 catcattgat gagggttgat tatcacagtt tattactctg aattggctat ccgcgtgtgt
     5761 acctctacct ggagtttttc ccacggtgga tatttcttct tgcgctgagc gtaagagcta
     5821 tctgacagaa cagttcttct ttgcttcctc gccagttcgc tcgctatgct cggttacacg
     5881 gctgcggcga gcgctagtga taataagtga ctgaggtatg tgctcttctt atctcctttt
     5941 gtagtgttgc tcttatttta aacaactttg cggttttttg atgactttgc gattttgttg
     6001 ttgctttgca gtaaattgca agatttaata aaaaaacgca aagcaatgat taaaggatgt
     6061 tcagaatgaa actcatggaa acacttaacc agtgcataaa cgctggtcat gaaatgacga
     6121 aggctatcgc cattgcacag tttaatgatg acagcccgga agcgaggaaa ataacccggc
     6181 gctggagaat aggtgaagca gcggatttag ttggggtttc ttctcaggct atcagagatg
     6241 ccgagaaagc agggcgacta ccgcacccgg atatggaaat tcgaggacgg gttgagcaac
     6301 gtgttggtta tacaattgaa caaattaatc atatgcgtga tgtgtttggt acgcgattgc
     6361 gacgtgctga agacgtattt ccaccggtga tcggggttgc tgcccataaa ggtggcgttt
     6421 acaaaacctc agtttctgtt catcttgctc aggatctggc tctgaagggg ctacgtgttt
     6481 tgctcgtgga aggtaacgac ccccagggaa cagcctcaat gtatcacgga tgggtaccag
     6541 atcttcatat tcatgcagaa gacactctcc tgcctttcta tcttggggaa aaggacgatg
     6601 tcacttatgc aataaagccc acttgctggc cggggcttga cattattcct tcctgtctgg
     6661 ctctgcaccg tattgaaact gagttaatgg gcaaatttga tgaaggtaaa ctgcccaccg
     6721 atccacacct gatgctccga ctggccattg aaactgttgc tcatgactat gatgtcatag
     6781 ttattgacag cgcgcctaac ctgggtatcg gcacgattaa tgtcgtatgt gctgctgatg
     6841 tgctgattgt tcccacgcct gctgagttgt ttgactacac ctccgcactg cagtttttcg
     6901 atatgcttcg tgatctgctc aagaacgttg atcttaaagg gttcgagcct gatgtacgta
     6961 ttttgcttac caaatacagc aatagcaatg gctctcagtc cccgtggatg gaggagcaaa
     7021 ttcgggatgc ctggggaagc atggttctaa aaaatgttgt acgtgaaacg gatgaagttg
     7081 gtaaaggtca gatccggatg agaactgttt ttgaacaggc cattgatcaa cgctcttcaa
     7141 ctggtgcctg gagaaatgct ctttctattt gggaacctgt ctgcaatgaa attttcgatc
     7201 gtctgattaa accacgctgg gagattagat aatgaagcgt gcgcctgtta ttccaaaaca
     7261 tacgctcaat actcaaccgg ttgaagatac ttcgttatcg acaccagctg ccccgatggt
     7321 ggattcgtta attgcgcgcg taggagtaat ggctcgcggt aatgccatta ctttgcctgt
     7381 atgtggtcgg gatgtgaagt ttactcttga agtgctccgg ggtgatagtg ttgagaagac
     7441 ctctcgggta tggtcaggta atgaacgtga ccaggagctg cttactgagg acgcactgga
     7501 tgatctcatc ccttcttttc tactgactgg tcaacagaca ccggcgttcg gtcgaagagt
     7561 atctggtgtc atagaaattg ccgatgggag tcgccgtcgt aaagctgctg cacttaccga
     7621 aagtgattat cgtgttctgg ttggcgagct ggatgatgag cagatggctg cattatccag
     7681 attgggtaac gattatcgcc caacaagtgc ttatgaacgt ggtcagcgtt atgcaagccg
     7741 attgcagaat gaatttgctg gaaatatttc tgcgctggct gatgcggaaa atatttcacg
     7801 taagattatt acccgctgta tcaacaccgc caaattgcct aaatcagttg ttgctctttt
     7861 ttctcacccc ggtgaactat ctgcccggtc aggtgatgca cttcaaaaag cctttacaga
     7921 taaagaggaa ttacttaagc agcaggcatc taaccttcat gagcagaaaa aagctggggt
     7981 gatatttgaa gctgaagaag ttatcactct tttaacttct gtgcttaaaa cgtcatctgc
     8041 atcaagaact agtttaagct cacgacatca gtttgctcct ggagcgacag tattgtataa
     8101 gggcgataaa atggtgctta acctggacag gtctcgtgtt ccaactgagt gtatagagaa
     8161 aattgaggcc attcttaagg aacttgaaaa gccagcaccc tgatgcgacc acgttttagt
     8221 ctacgtttat ctgtctttac ttaatgtcct ttgttacagg ccagaaagca taactggcct
     8281 gaatattctc tctgggccca ctgttccact tgtatcgtcg gtctgataat cagactggga
     8341 ccacggtccc actcgtatcg tcggtctgat tattagtctg ggaccacggt cccactcgta
     8401 tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgataatc
     8461 agactgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccatggtc
     8521 ccactcgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg tatcgtcggt
     8581 ctgattatta gtctggaacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg
     8641 accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacga tcccactcgt
     8701 gttgtcggtc tgattatcgg tctgggacca cggtcccact tgtattgtcg atcagactat
     8761 cagcgtgaga ctacgattcc atcaatgcct gtcaagggca agtattga
//
