LOCUS       40924_37063        9168 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Agrobacterium binary vector with a multiple cloning site (MCS), for 
            plant cell transformation. The MCS is reversed in pRI 909.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9168)
  AUTHORS   TaKaRa
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9168)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(57..645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    889..5524
                     /label=pRi replicator region
                     /note="replicator region from Agrobacterium plasmid pRiA
                     4b"
     misc_feature    5531..5555
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(5893..6145)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6422..7210)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(7234..7417)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    7584..7605
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7620..7650
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7658..7674
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7682..7698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7708..7764
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7768..7784)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7899..7923
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(8010..8801)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
       61 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      481 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      781 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      841 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactcga tcctacaagg
      901 tagaatccgc ctgagtcgca agggtgactt cgcctatatt ggacgacggc gcgcagaggg
      961 cgacctcttt ttgggttacg attgtaggat tatcactaaa acaatacatg aacatattca
     1021 aatggcaatc tctctaaggc attggaaata aatacaaata acagttgggt ggagtttttc
     1081 gacctgaggg cgttaacctt ctgttaacct aaaagctctt gcccaaacag cagaatcggc
     1141 gctaattgcc agcggcggaa cttttccagt ttcgcgaaaa atatcgccac tggcaaggaa
     1201 tgggtttgag atggcgaagt ctgtcctaaa agcagcgcct gtagttgtag ggttgacggc
     1261 cttgatggag cgtcatgccg atgccctctc gagccaactt caagcacatc atcttaaggt
     1321 tttcccgccg cattccgaga agggtattcg aacattcggg ccatcggagg cgtccaagct
     1381 gctcggcgtt ggcgagtcat atttacggca gaccgcgtct gagatgccag agttgaatgt
     1441 tagcatgagc ccaggtggca ggcgaatgtt ctcaattgaa gatatccatg tgattcggaa
     1501 gtatatggat caggtcggcc gcgggaaccg gcgctacctg ccacatcgtc gaggcggcga
     1561 gcagcttcag gttatctctg tgatgaattt caaaggtggg tcgggtaaga ccaccaccgc
     1621 cgcgcatctg gcgcagtacc tcgctatgcg cggatatcga gtcttggcca ttgatctcga
     1681 tcctcaagcg agcctttctg cactctttgg gagccaaccg gagacggacg ttggcccgaa
     1741 cgaaacgctc tacggcgcta taaggtatga tgatgagcag gtggcaatcg aacgagtcgt
     1801 ccgagggact tacattcccg acctccacct gattcctggt aaccttgagc tgatggagtt
     1861 tgaacacgat acgccacgcg cgctgatgaa ccgcaaagag ggcgacacgc tcttttatgg
     1921 tcgcatcagc caagtaattg aagatatcgc ggataactat gacgtcgtgg tcatcgactg
     1981 ccctccccag cttgggtatc tcacgctatc cgcattgact gcggcgacgt ccattcttgt
     2041 cacggtccat ccgcagatgc tggatgtgat gtcgatgaac cagtttctgg caatgacatc
     2101 gaaccttttg cgtgaaatcg agaatgctgg cgccaagttc aagtttaatt ggatgcgcta
     2161 tctgataacc cgtttcgaac cgagcgacgg accacagaac caaatggtag gttatctgcg
     2221 gtcgattttt ggcgaaaatg tcctcaattt tccgatgctt aaaaccaccg cggtttcgga
     2281 cgctggcctg acaaaccaga ctctattcga agtggagcgt ggcctgttca cgcgctcgac
     2341 ctatgatcga gccttggagg cgatgaacgc cgtcaacgac gagatcgaaa cactgatcaa
     2401 aaaagcatgg ggtaggccca catgagccgg aagcacatcc ttggcgtctc aactgacgcc
     2461 cctgagacgt cgcccgccga caataggacg gcaaagaacc gctccatgcc gctcctcggc
     2521 gtaacaagga aggagcgcga tccggcaacg aagctcacag cgaacattgg taacgcactg
     2581 cgagagcaaa acgatcgtct tagccgtgcc gaagagatcg agcggcgtct cgctgaaggt
     2641 caggcagtga tagagttgga tgcctcgtca atagaaccgt ctttcgtgca ggatcgtatg
     2701 cgaggggaca ttgacgggct ccttacttcg atccgggaac aaggacagca agtcccaatc
     2761 cttgtgcgac cgcatccgag ccagccgggc cgatatcagg ttgccttcgg ccaccgccgg
     2821 ctacgcgccg tttcagaact cggacttccg gtcagggcgg tcgttcgcga actgacggac
     2881 gagcaagtgg tcgtagcaca gggtcaggaa aacaatgtgc gcgaagatct taccttcatc
     2941 gaaaaggcgc gcttcgcaca tcgcctgaac aggcagtttt ctcgagagat tgtcatcgcc
     3001 gcgatgtcga tcgacaagag caatttgtcc aagatgcttc tgctcgttga cgccctcccc
     3061 tctgaactga ccgatgctat tggtgccgct cctggtgttg gacggccgag ttggcaacaa
     3121 cttgccgagc tgattgagaa agtttcttca ccggccgacg tggctaaata tgctatgtcg
     3181 gaggaagttc aagcgctgcc atcggcagaa cgattcaagg cggtgatcgc tagtctgaag
     3241 cccagtcggg ttgcgcgtgg acttcccgag gtcatggcca ccccagacgg caccagaatt
     3301 gcacaggtga cgcagagcaa ggccaaactg gaaatcacga ttgacaggaa ggcgacgccc
     3361 gattttgcga ccttcgtgct cgatcatgtg ccagcgctgt atcaagcgta ccacgctgag
     3421 aaccaacgga aacggggaga gtaaaccgca aaagaaaaga gccccctcaa cgtcgccgtc
     3481 gcggaagccc ttctgtctct ctagcgcgaa cagaatcgca tttcctcgaa tcctcgtcaa
     3541 gagtttttag cgccgttttg gtgagctgat ttcctttgcc tgctgaaagg tgaaagatga
     3601 tgcagacagg aagtgtaacg acgccattcg ggcggcggcc aatgacgctt gcgcttgtgc
     3661 ggcgccagac ggcgctggcc gatatcaaac aaggcaagac agcggacaag tggaaggtct
     3721 ttagggacgc gtccgcggcc atggaactac ttggaatcca gtccaacagt cttgccgtcc
     3781 ttgatgcgct attgagcttt cacccggaaa cggagttgcg tcaggaggca cagctgatcg
     3841 tcttcccgtc gaatgctcag cttgcccttc gggcgcatgg gatggctggc gcgactttgc
     3901 gtaggcacat cgccatgctc gtggagtcag gcttgatcgt ccggaaggat agcgccaacg
     3961 gaaagcgtta cgctcgtaag gatggcgctg gtcagatcga gcgcgcgttt ggcttcgatt
     4021 tgtctccgct tctcgcgcgg tccgaagagc tagcgatgat ggcacagcag gtgatggccg
     4081 atcgagcagc attcaggatg gccaaagaaa gtctgacgat ttgccgacgg gacgttcgga
     4141 agctaattac ggcagctatg gaagagggag cggagggcga ctggcaagct gtcgaggaag
     4201 tctatgtgga acttgtgggt agaatcccac gcgccccgac gcttgctgat gtagagtcaa
     4261 ttctcgaaga gatgtggatg ctccaggaag agataatcaa ccggttggaa attagagaca
     4321 attcagaaaa taatagcacc aatgctgccc agagcgagca gcacatacag aactcaaaac
     4381 ccgaatccgt taatgaactt gaacctcgct ctgaaaagga gcagggcgct aagccgagtg
     4441 aaatagaccg ggcaaggagc gagccgataa aagcgttccc cctcgggatg atcctgaaag
     4501 cgtgcccgac cattggcaat tatgggccga gcggtgcggt tgctagctgg cgtgacctca
     4561 tgtcggctgc ggtggtggtt cggtctatgc tgggggtcag cccgtcggct taccaagacg
     4621 cgtgtgaggc aatgggaccg gagaatgcgg cagcagcgat ggcgtgcatt ttggagcgag
     4681 cgaacttcat caattcgcct gggggctatc tccgagatct gacacggcgg agcgaactcg
     4741 ggaagttttc acttggcccg atgataatgg cgctcttgaa ggctagcggg caggggacgt
     4801 tgcggtttgg ctagaattag cgagtatgga gcaggatggt ctgtggtcag ctgaccacag
     4861 acctaatagg ttgaaaacat gagcgttttt tggatgatcg acagaccatc cgattcccgg
     4921 agtaccaagc gtgctctgat gggagcgata acattactca acaagcacga aggccccatg
     4981 ccgatcgttg atcgtgaagg agagcctgct ctacatgcgg cggtattttg ccggccgagg
     5041 catgtagtcg cggagcactg cctatttact gccctaggca caaacgttga ctcttggatc
     5101 gagctggcag acaaagcaat aacccacaca gaggacgatt aatggctgac gaagagatcc
     5161 agaatccgcc ggacggtact gctgctgccg aagttgagcc ggctgctcct agaggtagaa
     5221 gagcaaagaa agcaccagcc gaaacagccc gcacgggatc gttcaaatcc gtgaagccga
     5281 aaacccgcgg cctcagcaac cgagaaaaac tggagaagat cggtcaaatc gaagctcagg
     5341 tcgctggcgg cgcaaccttg aaggacgccg ttaagatcgt gggtatttcc gttcagacct
     5401 attatcaatg gaagagagct gcggttcaac ctgtctcaca gaatccggcc gtgtctgttt
     5461 cagttgacga tgaactcggc gagttcatcc aactcgagga ggaaaatcgg cggctcagaa
     5521 agctagcttg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
     5581 acacattgcg gacgttttta atgtactggg gtggtttttc ttttcaccag tgagacgggc
     5641 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     5701 gtttgcccca gcaggcgaaa atcctgtttg atggtggttc cgaaatcggc aaaatccctt
     5761 ataaatcaaa agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     5821 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     5881 gcccaaattc ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg
     5941 cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa
     6001 aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat
     6061 tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac
     6121 tttattgcca aatgtttgaa cgatcggggc ccgatcatat tgtcgctcag gatcgtggcg
     6181 ttgtgcttgt cggccgttgc tgtcgtaatg atatcggcac cttcgaccgc ctgttccgca
     6241 gagatcccgt gggcgaagaa ctccagcatg agatccccgc gctggaggat catccagccg
     6301 gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt ggaatcgaaa
     6361 tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc
     6421 agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac
     6481 cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg
     6541 tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc
     6601 cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga
     6661 cgagatcatc gccgtcgggc atacgcgcct tgagcctggc gaacagttcg gctggcgcga
     6721 gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc atccgagtac
     6781 gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg
     6841 tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag
     6901 atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag
     6961 tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg
     7021 ctgcctcgtc ctgtagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg
     7081 ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg
     7141 cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat
     7201 cttgttcaat catgcgaaac gatccagatc cggtgcagat tatttggatt gagagtgaat
     7261 atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc atttttgaca
     7321 agaaatattt gctagctgat agtgacctta ggcgactttt gaacgcgcaa taatggtttc
     7381 tgacgtatgt gcttagctca ttaaactcca gaaacccgcg gctgagtggc tccttcaacg
     7441 ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc gcgtcatcgg cgggggtcat
     7501 aacgtgactc ccttaattct ccgctcatga tcctggcacg acaggtttcc cgactggaaa
     7561 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7621 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7681 acaggaaaca gctatgacca tgattacgaa ttcgagctcg gtacccgggg atcctctaga
     7741 gtcgacctgc aggcatgcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     7801 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccaggatcag
     7861 attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata ttggcgggta
     7921 aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc gtgaaaaggt
     7981 ttatccgttc gtccatttgt atgtgactaa aacaattcat ccagtaaaat ataatatttt
     8041 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct
     8101 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc
     8161 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg
     8221 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa
     8281 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc
     8341 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta
     8401 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac
     8461 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca
     8521 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt
     8581 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag
     8641 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc
     8701 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg
     8761 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt
     8821 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
     8881 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa
     8941 agttggcgta taacatagta tcgacggagc cgattttgaa accacaatta tgggtgatgc
     9001 tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt
     9061 ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg
     9121 ccggacatca gcgctatctc tgctctcact gccgtaaaac atggcaac
//
