LOCUS       40924_36973       11881 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRGK337, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Engineer CB, Fitzsimmons KC, Schmuke JJ, Dotson SB, Kranz RG.
  TITLE     Development and evaluation of a Gal4-mediated LUC/GFP/GUS enhancer 
            trap system in Arabidopsis
  JOURNAL   BMC Plant Biol. 5, 9 (2005)
  PUBMED    15941484
REFERENCE   2  (bases 1 to 11881)
  AUTHORS   Engineer CB, Fitzsimmons KC, Schmuke J, Dotson S, Kranz RG.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2004) Biology, Washington University in St. Louis,
            1 Brookings Drive, Biology 1137, St. Louis, MO 63130-4899, USA
REFERENCE   3  (bases 1 to 11881)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11881)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Plant 
            Biol. 5, 9 (2005)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-SEP-2004) Biology, Washington University in St. Louis, 1 
            Brookings Drive, Biology 1137, St. Louis, MO 63130-4899, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             553..2358
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
                     VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
                     EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
                     FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
                     VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQFL
                     INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
                     DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
                     NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     CDS             2365..2382
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      2414..2666
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2688..2712
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4012..4638
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5075..6139
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6208..6402
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6746..6886
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7072..7660)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7750..8541)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8966..8990
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9068..9242)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9285..10307)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10375..11052)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11243..11264
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11279..11309
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11317..11333
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11341..11357
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11413..11507
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        11568..11614
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             join(11717..11881,1..546)
                     /codon_start=1
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
ORIGIN
        1 tggccaacac ttgtcactac tttcacttat ggtgttcaat gcttttcaag atacccagat
       61 catatgaagc ggcacgactt cttcaagagc gccatgcctg agggatacgt gcaggagagg
      121 accatcttct tcaaggacga cgggaactac aagacacgtg ctgaagtcaa gtttgaggga
      181 gacaccctcg tcaacaggat cgagcttaag ggaatcgatt tcaaggagga cggaaacatc
      241 ctcggccaca agttggaata caactacaac tcccacaacg tatacatcat ggccgacaag
      301 caaaagaacg gcatcaaagc caacttcaag acccgccaca acatcgaaga cggcggcgtg
      361 caactcgctg atcattatca acaaaatact ccaattggcg atggccctgt ccttttacca
      421 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
      481 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
      541 tacaaagcta gtttacgtcc tgtagaaacc ccaacccgtg aaatcaaaaa actcgacggc
      601 ctgtgggcat tcagtctgga tcgcgaaaac tgtggaattg atcagcgttg gtgggaaagc
      661 gcgttacaag aaagccgggc aattgctgtg ccaggcagtt ttaacgatca gttcgccgat
      721 gcagatattc gtaattatgc gggcaacgtc tggtatcagc gcgaagtctt tataccgaaa
      781 ggttgggcag gccagcgtat cgtgctgcgt ttcgatgcgg tcactcatta cggcaaagtg
      841 tgggtcaata atcaggaagt gatggagcat cagggcggct atacgccatt tgaagccgat
      901 gtcacgccgt atgttattgc cgggaaaagt gtacgtatca ccgtttgtgt gaacaacgaa
      961 ctgaactggc agactatccc gccgggaatg gtgattaccg acgaaaacgg caagaaaaag
     1021 cagtcttact tccatgattt ctttaactat gccggaatcc atcgcagcgt aatgctctac
     1081 accacgccga acacctgggt ggacgatatc accgtggtga cgcatgtcgc gcaagactgt
     1141 aaccacgcgt ctgttgactg gcaggtggtg gccaatggtg atgtcagcgt tgaactgcgt
     1201 gatgcggatc aacaggtggt tgcaactgga caaggcacta gcgggacttt gcaagtggtg
     1261 aatccgcacc tctggcaacc gggtgaaggt tatctctatg aactgtgcgt cacagccaaa
     1321 agccagacag agtgtgatat ctacccgctt cgcgtcggca tccggtcagt ggcagtgaag
     1381 ggccaacagt tcctgattaa ccacaaaccg ttctacttta ctggctttgg tcgtcatgaa
     1441 gatgcggact tacgtggcaa aggattcgat aacgtgctga tggtgcacga ccacgcatta
     1501 atggactgga ttggggccaa ctcctaccgt acctcgcatt acccttacgc tgaagagatg
     1561 ctcgactggg cagatgaaca tggcatcgtg gtgattgatg aaactgctgc tgtcggcttt
     1621 cagctgtctt taggcattgg tttcgaagcg ggcaacaagc cgaaagaact gtacagcgaa
     1681 gaggcagtca acggggaaac tcagcaagcg cacttacagg cgattaaaga gctgatagcg
     1741 cgtgacaaaa accacccaag cgtggtgatg tggagtattg ccaacgaacc ggatacccgt
     1801 ccgcaaggtg cacgggaata tttcgcgcca ctggcggaag caacgcgtaa actcgacccg
     1861 acgcgtccga tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga taccatcagc
     1921 gatctctttg atgtgctgtg cctgaaccgt tattacggat ggtatgtcca aagcggcgat
     1981 ttggaaacgg cagagaaggt actggaaaaa gaacttctgg cctggcagga gaaactgcat
     2041 cagccgatta tcatcaccga atacggcgtg gatacgttag ccgggctgca ctcaatgtac
     2101 accgacatgt ggagtgaaga gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt
     2161 gatcgcgtca gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt tgcgacctcg
     2221 caaggcatat tgcgcgttgg cggtaacaag aaagggatct tcactcgcga ccgcaaaccg
     2281 aagtcggcgg cttttctgct gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg
     2341 cagcagggag gcaaacaagc tagccaccac caccaccacc acgtgtgaat tggtgaccag
     2401 ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     2461 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     2521 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     2581 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     2641 cgcggtgtca tctatgttac tagatcggga attaaactat cagtgtttga caggatatat
     2701 tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt aaaagggcgt
     2761 gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt tcccctcggg
     2821 atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc tgacgttcag
     2881 tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga caggctgccg
     2941 ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt
     3001 gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct
     3061 atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg
     3121 ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc
     3181 tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc
     3241 gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag gaggccggcg
     3301 cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg
     3361 tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc
     3421 ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca
     3481 ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag
     3541 aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg
     3601 aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg
     3661 aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca
     3721 ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc
     3781 ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct
     3841 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
     3901 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     3961 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     4021 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     4081 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     4141 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     4201 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     4261 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     4321 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     4381 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     4441 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     4501 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
     4561 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
     4621 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
     4681 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
     4741 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
     4801 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
     4861 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
     4921 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt
     4981 ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc
     5041 actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca
     5101 aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca
     5161 gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg
     5221 aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc
     5281 caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga
     5341 tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg
     5401 cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg
     5461 catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc
     5521 catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt
     5581 tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt
     5641 agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa
     5701 gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt
     5761 aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg
     5821 cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat
     5881 cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga
     5941 agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa
     6001 gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa
     6061 ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg
     6121 cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg
     6181 ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc
     6241 gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc
     6301 acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt
     6361 aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac
     6421 agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc
     6481 ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa
     6541 tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg
     6601 caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     6661 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     6721 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     6781 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     6841 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     6901 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6961 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7021 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7081 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7141 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7201 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7261 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7321 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7381 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7441 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7501 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7561 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7621 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7681 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc
     7741 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
     7801 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
     7861 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
     7921 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
     7981 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
     8041 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
     8101 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
     8161 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
     8221 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
     8281 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
     8341 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
     8401 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
     8461 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
     8521 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
     8581 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
     8641 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
     8701 tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg tcatcgttac
     8761 aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc ttagttgccg
     8821 ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg gctctcccgc
     8881 tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc
     8941 ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac aaattgacgc
     9001 ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac gccgaattaa
     9061 ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat
     9121 agaagtattt tacaaataca aatacatact aagggtttct tatatgctca acacatgagc
     9181 gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga
     9241 aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt tgccctcgga
     9301 cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag
     9361 acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg
     9421 attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc
     9481 tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg cgatcctgca
     9541 agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc
     9601 ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag
     9661 tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg
     9721 tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc
     9781 tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga
     9841 tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg
     9901 aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catagcctcc
     9961 gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc
    10021 tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat gtcaagcact
    10081 tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa
    10141 ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa
    10201 gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg
    10261 atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc tcattgcccc
    10321 cccggatctg cgaaagctcg agagagatag atttgtagag agagactggt gatttcagcg
    10381 tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga aggatagtgg
    10441 gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac
    10501 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
    10561 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
    10621 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
    10681 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
    10741 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgtta
    10801 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
    10861 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg aacgatagcc
    10921 tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac tgtccttttg
    10981 atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat taccctttgt
    11041 tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt cttggagtag
    11101 acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc aaaccgcctc
    11161 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    11221 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    11281 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    11341 caggaaacag ctatgacatg attacgaatt cgagctcggt accgattacg ccaagcttgc
    11401 atgcctgcag gtcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
    11461 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agactctaga
    11521 agctactcca cgtccataag ggacacatca caatcccact atccttcgca agacccttcc
    11581 tctatataag gaagttcatt tcatttggag aggacgacct gcaggtcgac ggatccaagg
    11641 agatataaca atgaagacta atctttttct ctttctcatc ttttcacttc tcctatcatt
    11701 atcctcggcc gaattcagta aaggagaaga acttttcact ggagttgtcc caattcttgt
    11761 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
    11821 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
    11881 a
//
