LOCUS       pRGEB32.       15905 bp DNA     circular SYN 06-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pRGEB32
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15905)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 15905)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..15905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             26..49
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     terminator      71..323
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(332..348)
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(349..365)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    568..592
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1892..2518
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2955..4019
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      4088..4282
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4626..4766
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4952..5540)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5630..6421)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6846..6870
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6948..7122)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7165..8187)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(8255..8932)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    9123..9144
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9159..9189
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9197..9213
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9221..9237
                     /label=M13 rev
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9256..9636
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     misc_RNA        9653..9728
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     intron          10557..11518
                     /label=intron
                     /note="intron upstream of the coding sequence for the rice 
                     polyubiquitin gene RUBQ2"
     protein_bind    11529..11553
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             11598..11663
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=three tandem FLAG
                     /note="3xFLAG"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             11670..11690
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T
                     antigen"
                     /label=nuclear localization signal of SV40 large T
                     ant
                     /note="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             11715..15815
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     CDS             15816..15863
                     /codon_start=1
                     /product="bipartite nuclear localization signal from
                     nucleoplasmin"
                     /label=bipartite nuclear localization signal from
                     nucl
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cttgtacaaa gtggttgata acagcgacta caaggatgac gatgacaagg cttagagctc
       61 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
      121 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
      181 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
      241 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
      301 ggtgtcatct atgttactag atcgggaatt cactggccgt cgttttacac tggccgtcgt
      361 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
      421 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
      481 gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga ttgtcgtttc
      541 ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa acctaagaga
      601 aaagagcgtt tattagaata acggatattt aaaagggcgt gaaaaggttt atccgttcgt
      661 ccatttgtat gtgcatgcca accacagggt tcccctcggg atcaaagtac tttgatccaa
      721 cccctccgct gctatagtgc agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac
      781 gacatgtcgc acaagtccta agttacgcga caggctgccg ccctgccctt ttcctggcgt
      841 tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa ccggagacat
      901 tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt cagcaccgac
      961 gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac caagctgttt
     1021 tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat gcttgaccac
     1081 ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg cagcacccgc
     1141 gacctactgg acattgccga gcgcatccag gaggccggcg cgggcctgcg tagcctggca
     1201 gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc
     1261 attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc
     1321 aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca gatcgcgcac
     1381 gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc actgcttggc
     1441 gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag
     1501 gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc cctggcggcc
     1561 gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc caggacgaac
     1621 cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc
     1681 cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg tctgatgcca
     1741 agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa
     1801 ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat
     1861 gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac ttaaccagaa
     1921 aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg
     1981 ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt gggcggccgt
     2041 gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt
     2101 gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg cggcggactt
     2161 ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc caagccctta
     2221 cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg aggtcacgga
     2281 tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg
     2341 tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc gtatcacgca
     2401 gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat cagaacccga
     2461 gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa aactcatttg
     2521 agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg
     2581 agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc agccatgaag
     2641 cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta cgcggtacgc
     2701 caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc agagtaaatg
     2761 agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca tggaaaatca
     2821 agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc
     2881 aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc ccaagcccga
     2941 ggaatcggcg tgacggtcgc aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg
     3001 atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc atcgaggcag
     3061 aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc
     3121 aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac gagcaaccag
     3181 attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc atcatggacg
     3241 tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc
     3301 ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt gtgtgggatt
     3361 acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga taccgggaag
     3421 ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct
     3481 gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc attcggttaa
     3541 acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc ctggtgacgg
     3601 tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa accgggcggc
     3661 cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga
     3721 acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc atcggccgtt
     3781 ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga
     3841 cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc accgtgcgca
     3901 agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg gggcaggctg
     3961 gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc gccggttcct
     4021 aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt cgaaaagcac
     4081 tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg aaccggaacc
     4141 cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa gtgactgata
     4201 taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt aaaactctta
     4261 aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag ctgcaaaaag
     4321 cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg
     4381 ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg cgcggacaag
     4441 ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc
     4501 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     4561 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     4621 cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg
     4681 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     4741 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
     4801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     5401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5581 ggaacgaaaa ctcacgttaa gggattttgg tcatgcattc taggtactaa aacaattcat
     5641 ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt aagtcaaaaa
     5701 atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg cagaaggcaa
     5761 tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca cttactttgc
     5821 catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt
     5881 cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat ggagtgtctt
     5941 cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa tccaattcgg
     6001 ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga
     6061 gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca tcttcatact
     6121 cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag ccatcatgcc
     6181 gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc atcatgtcct
     6241 tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt tttaaatata
     6301 ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct tccgtatctt
     6361 ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc attttagcca
     6421 tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag ctaattataa
     6481 caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc agaaaacagc
     6541 tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc cgattttgaa
     6601 accgcggtga tcacaggcag caacgctctg tcatcgttac aatcaacatg ctaccctccg
     6661 cgagatcatc cgtgtttcaa acccggcagc ttagttgccg ttcttccgaa tagcatcggt
     6721 aacatgagca aagtctgccg ccttacaacg gctctcccgc tgacgccgtc ccggactgat
     6781 gggctgcctg tatcgagtgg tgattttgtg ccgagctgcc ggtcggggag ctgttggctg
     6841 gctggtggca ggatatattg tggtgtaaac aaattgacgc ttagacaact taataacaca
     6901 ttgcggacgt ttttaatgta ctgaattaac gccgaattaa ttcgggggat ctggatttta
     6961 gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt tacaaataca
     7021 aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat aggaacccta
     7081 attcccttat ctgggaacta ctcacacatt attatggaga aactcgagct tgtcgatcga
     7141 cagatccggt cggcatctac tctatttctt tgccctcgga cgagtgctgg ggcgtcggtt
     7201 tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc
     7261 gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg
     7321 cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc
     7381 aatgcggagc atatacgccc ggagtcgtgg cgatcctgca agctccggat gcctccgctc
     7441 gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc
     7501 gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg
     7561 gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc
     7621 ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac
     7681 ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa
     7741 atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt
     7801 ctggctaaga tcggccgcag cgatcgcatc catagcctcc gcgaccggtt gtagaacagc
     7861 gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca
     7921 ataggtcagg ctctcgctaa actccccaat gtcaagcact tccggaatcg ggagcgcggc
     7981 cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac
     8041 ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc
     8101 cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga
     8161 cgtcgcggtg agttcaggct ttttcatatc tcattgcccc ccgggatctg cgaaagctcg
     8221 agagagatag atttgtagag agagactggt gatttcagcg tgtcctctcc aaatgaaatg
     8281 aacttcctta tatagaggaa ggtcttgcga aggatagtgg gattgtgcgt catcccttac
     8341 gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
     8401 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
     8461 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
     8521 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
     8581 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
     8641 attcttggag tagacgagag tgtcgtgctc caccatgtta tcacatcaat ccacttgctt
     8701 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
     8761 tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg
     8821 gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg
     8881 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
     8941 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
     9001 catgttggca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     9061 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     9121 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     9181 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     9241 gattacgcca agcttaagga atctttaaac atacgaacag atcacttaaa gttcttctga
     9301 agcaacttaa agttatcagg catgcatgga tcttggagga atcagatgtg cagtcaggga
     9361 ccatagcaca agacaggcgt cttctactgg tgctaccagc aaatgctgga agccgggaac
     9421 actgggtacg ttggaaacca cgtgatgtga agaagtaaga taaactgtag gagaaaagca
     9481 tttcgtagtg ggccatgaag cctttcagga catgtattgc agtatgggcc ggcccattac
     9541 gcaattggac gacaacaaag actagtatta gtaccacctc ggctatccac atagatcaaa
     9601 gctgatttaa aagagttgtg cagatgatcc gtggcaggag accgaggtct cggttttaga
     9661 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
     9721 gtcggtgctt ttttgtttta gagctagaaa tagcaagtta aaataaggct agtccgtttt
     9781 tagcgcgtgc atgcctgcag gtccacaaat tcgggtcaag gcggaagcca gcgcgccacc
     9841 ccacgtcagc aaatacggag gcgcggggtt gacggcgtca cccggtccta acggcgacca
     9901 acaaaccagc cagaagaaat tacagtaaaa aaaaagtaaa ttgcactttg atccaccttt
     9961 tattacctaa gtctcaattt ggatcaccct taaacctatc ttttcaattt gggccgggtt
    10021 gtggtttgga ctaccatgaa caacttttcg tcatgtctaa cttccctttc agcaaacata
    10081 tgaaccatat atagaggaga tcggccgtat actagagctg atgtgtttaa ggtcgttgat
    10141 tgcacgagaa aaaaaaatcc aaatcgcaac aatagcaaat ttatctggtt caaagtgaaa
    10201 agatatgttt aaaggtagtc caaagtaaaa cttatagata ataaaatgtg gtccaaagcg
    10261 taattcactc aaaaaaaatc aacgagacgt gtaccaaacg gagacaaacg gcatcttctc
    10321 gaaatttccc aaccgctcgc tcgcccgcct cgtcttcccg gaaaccgcgg tggtttcagc
    10381 gtggcggatt ctccaagcag acggagacgt cacggcacgg gactcctccc accacccaac
    10441 cgccataaat accagccccc tcatctcctc tcctcgcatc agctccaccc ccgaaaaatt
    10501 tctccccaat ctcgcgaggc tctcgtcgtc gaatcgaatc ctctcgcgtc ctcaaggtac
    10561 gctgcttctc ctctcctcgc ttcgtttcga ttcgatttcg gacgggtgag gttgttttgt
    10621 tgctagatcc gattggtggt tagggttgtc gatgtgatta tcgtgagatg tttaggggtt
    10681 gtagatctga tggttgtgat ttgggcacgg ttggttcgat aggtggaatc gtggttaggt
    10741 tttgggattg gatgttggtt ctgatgattg gggggaattt ttacggttag atgaattgtt
    10801 ggatgattcg attggggaaa tcggtgtaga tctgttgggg aattgtggaa ctagtcatgc
    10861 ctgagtgatt ggtgcgattt gtagcgtgtt ccatcttgta ggccttgttg cgagcatgtt
    10921 cagatctact gttccgctct tgattgagtt attggtgcca tgggttggtg caaacacagg
    10981 ctttaatatg ttatatctgt tttgtgtttg atgtagatct gtagggtagt tcttcttaga
    11041 catggttcaa ttatgtagct tgtgcgtttc gatttgattt catatgttca cagattagat
    11101 aatgatgaac tcttttaatt aattgtcaat ggtaaatagg aagtcttgtc gctatatctg
    11161 tcataatgat ctcatgttac tatctgccag taatttatgc taagaactat attagaatat
    11221 catgttacaa tctgtagtaa tatcatgtta caatctgtag ttcatctata taatctattg
    11281 tggtaatttc tttttactat ctgtgtgaag attattgcca ctagttcatt ctacttattt
    11341 ctgaagttca ggatacgtgt gctgttacta cctatctgaa tacatgtgtg atgtgcctgt
    11401 tactatcttt ttgaatacat gtatgttctg ttggaatatg tttgctgttt gatccgttgt
    11461 tgtgtcctta atcttgtgct agttcttacc ctatctgttt ggtgattatt tcttgcagat
    11521 agttatcaac aagtttgtac aaaaaagcag gcttcgaagg agatagaacc aattctctaa
    11581 ggaaatactt aaccatggac tataaggacc acgacggaga ctacaaggat catgatattg
    11641 attacaaaga cgatgacgat aagatggccc caaagaagaa gcggaaggtc ggtatccacg
    11701 gagtcccagc agccgacaag aagtacagca tcggcctgga catcggcacc aactctgtgg
    11761 gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag gtgctgggca
    11821 acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc gacagcggcg
    11881 aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc agacggaaga
    11941 accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg gacgacagct
    12001 tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac gagcggcacc
    12061 ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc accatctacc
    12121 acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg atctatctgg
    12181 ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac ctgaaccccg
    12241 acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac cagctgttcg
    12301 aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct gccagactga
    12361 gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag aagaatggcc
    12421 tgttcggaaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag agcaacttcg
    12481 acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac gacctggaca
    12541 acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc aagaacctgt
    12601 ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc aaggcccccc
    12661 tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc ctgctgaaag
    12721 ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac cagagcaaga
    12781 acggctacgc cggctacatt gacggcggag ccagccagga agagttctac aagttcatca
    12841 agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg aacagagagg
    12901 acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag atccacctgg
    12961 gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg aaggacaacc
    13021 gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc cctctggcca
    13081 ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc accccctgga
    13141 acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag cggatgacca
    13201 acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt
    13261 acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc
    13321 ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc
    13381 ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag tgcttcgact
    13441 ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca taccacgatc
    13501 tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag gacattctgg
    13561 aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag gaacggctga
    13621 aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg cggagataca
    13681 ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag cagtccggca
    13741 agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc atgcagctga
    13801 tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg tccggccagg
    13861 gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt aagaagggca
    13921 tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg
    13981 agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga cagaagaaca
    14041 gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc cagatcctga
    14101 aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg tactacctgc
    14161 agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg tccgactacg
    14221 atgtggacca tatcgtgcct cagagctttc tgaaggacga ctccatcgac aacaaggtgc
    14281 tgaccagaag cgacaagaac cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga
    14341 agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc cagagaaagt
    14401 tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag gccggcttca
    14461 tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag atcctggact
    14521 cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg aaagtgatca
    14581 ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac aaagtgcgcg
    14641 agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc
    14701 tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac aaggtgtacg
    14761 acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc gccaagtact
    14821 tcttctacag caacatcatg aactttttca agaccgagat taccctggcc aacggcgaga
    14881 tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg
    14941 gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa
    15001 agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag aggaacagcg
    15061 ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc ttcgacagcc
    15121 ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac
    15181 tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc ttcgagaaga
    15241 atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac ctgatcatca
    15301 agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg ctggcctctg
    15361 ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg aacttcctgt
    15421 acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag cagaaacagc
    15481 tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc agcgagttct
    15541 ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc tacaacaagc
    15601 accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt accctgacca
    15661 atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg aagaggtaca
    15721 ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc ggcctgtacg
    15781 agacacggat cgacctgtct cagctgggag gcgacaaaag gccggcggcc acgaaaaagg
    15841 ccggccaggc aaaaaagaaa aagtaagaat tcgcggccgc actcgagata tctagaccca
    15901 gcttt
//
