LOCUS       62056_18835       15041 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15041)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..15041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..386
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     misc_RNA        407..482
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1048..1393
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1415..1439
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1601..5701
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    complement(5782..5806)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5817..5840
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     terminator      5862..6114
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6359..6383
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7683..8309
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8746..9810
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     misc_feature    10417..10557
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10743..11331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11421..12212)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    12637..12661
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(12739..12913)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(12956..13978)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(14046..14723)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    14914..14935
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        14950..14980
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14988..15004
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     15012..15028
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 agcttaagga atctttaaac atacgaacag atcacttaaa gttcttctga agcaacttaa
       61 agttatcagg catgcatgga tcttggagga atcagatgtg cagtcaggga ccatagcaca
      121 agacaggcgt cttctactgg tgctaccagc aaatgctgga agccgggaac actgggtacg
      181 ttggaaacca cgtgatgtga agaagtaaga taaactgtag gagaaaagca tttcgtagtg
      241 ggccatgaag cctttcagga catgtattgc agtatgggcc ggcccattac gcaattggac
      301 gacaacaaag actagtatta gtaccacctc ggctatccac atagatcaaa gctgatttaa
      361 aagagttgtg cagatgatcc gtggcacaac tggcatgcga accaacgttt tagagctaga
      421 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
      481 gcttttttgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg tttttagcgc
      541 gtgcatgcct gcaggtcccc agattagcct tttcaatttc agaaagaatg ctaacccaca
      601 gatggttaga gaggcttacg cagcagcact catcaagacg atctacccga gcaataatct
      661 ccaggaaatc aaataccttc ccaagaaggt taaagatgca gtcaaaagat tcaggactaa
      721 ctgcatcaag aacacagaga aagatatatt tctcaagatc agaagtacta ttccagtatg
      781 gacgattcaa ggcttgcttc acaaaccaag gcaagtaata gagattggag tctctaaaaa
      841 ggtagttccc actgaatcaa aggccatgga gtcaaagatt caaatagagg acctaacaga
      901 actcgccgta aagactggcg aacagttcat acagagtctc ttacgactca atgacaagaa
      961 gaaaatcttc gtcaacatgg tggagcacga cacacttgtc tactccaaaa atatcaaaga
     1021 tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa tatccggaaa
     1081 cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag tggaaaagga
     1141 aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg aagatgcctc
     1201 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
     1261 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg acgtaaggga
     1321 tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa gttcatttca
     1381 tttggagaga acacggggga ctctagagtt atcaacaagt ttgtacaaaa aagcaggctt
     1441 cgaaggagat agaaccaatt ctctaaggaa atacttaacc atggactata aggaccacga
     1501 cggagactac aaggatcatg atattgatta caaagacgat gacgataaga tggccccaaa
     1561 gaagaagcgg aaggtcggta tccacggagt cccagcagcc gacaagaagt acagcatcgg
     1621 cctggacatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc
     1681 cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat
     1741 cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc
     1801 cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa
     1861 cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga
     1921 agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta
     1981 ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa
     2041 ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt
     2101 cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct
     2161 ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc
     2221 caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca
     2281 gctgcccggc gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct
     2341 gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa
     2401 ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga
     2461 cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt
     2521 gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca
     2581 ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa
     2641 agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag
     2701 ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga
     2761 actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg
     2821 cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga
     2881 tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat
     2941 cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa
     3001 gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc
     3061 ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct
     3121 gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa
     3181 atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat
     3241 cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta
     3301 cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa
     3361 cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga
     3421 caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga
     3481 cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat
     3541 gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa
     3601 cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt
     3661 cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat
     3721 ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc
     3781 cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt
     3841 gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca
     3901 gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat
     3961 caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa
     4021 cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact
     4081 ggacatcaac cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa
     4141 ggacgactcc atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga
     4201 caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa
     4261 cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct
     4321 gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac
     4381 aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa
     4441 gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa
     4501 ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta
     4561 cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt
     4621 cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga
     4681 aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac
     4741 cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga
     4801 aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag
     4861 catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga
     4921 gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc
     4981 taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa
     5041 agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat
     5101 catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa
     5161 agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa
     5221 cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct
     5281 gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc
     5341 ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga
     5401 gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga
     5461 caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa
     5521 tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga
     5581 caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat
     5641 ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggcga
     5701 caaaaggccg gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagt aagaattcgc
     5761 ggccgcactc gagatatcta gacccagctt tcttgtacaa agtggttgat aacagcgact
     5821 acaaggatga cgatgacaag gcttagagct cgaatttccc cgatcgttca aacatttggc
     5881 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     5941 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     6001 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     6061 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
     6121 tcactggccg tcgttttaca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     6181 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     6241 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga
     6301 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg
     6361 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt
     6421 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
     6481 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
     6541 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
     6601 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
     6661 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
     6721 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
     6781 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
     6841 ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg tgacagtgac
     6901 caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg agcgcatcca
     6961 ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca ccacgccggc
     7021 cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat
     7081 cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg
     7141 ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg
     7201 caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact
     7261 tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga
     7321 cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag aggaacaagc
     7381 atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg
     7441 gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg
     7501 catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc
     7561 gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg
     7621 cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac
     7681 gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa
     7741 cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc
     7801 cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg
     7861 gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag
     7921 tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact
     7981 tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg
     8041 agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt
     8101 cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg
     8161 agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg
     8221 ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc
     8281 tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa
     8341 aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt
     8401 ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga
     8461 tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc
     8521 tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt
     8581 tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat
     8641 gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc
     8701 cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgacggtcg caaaccatcc
     8761 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg
     8821 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
     8881 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gccgtcgatt
     8941 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
     9001 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
     9061 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
     9121 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
     9181 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
     9241 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
     9301 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
     9361 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
     9421 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
     9481 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
     9541 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
     9601 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
     9661 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
     9721 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
     9781 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
     9841 gccctagcag gggaaaaagg tcgaaaagca ctctttcctg tggatagcac gtacattggg
     9901 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
     9961 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
    10021 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
    10081 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
    10141 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
    10201 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
    10261 ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
    10321 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
    10381 tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc agtcacgtag
    10441 cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt actgagagtg
    10501 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
    10561 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    10621 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    10681 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    10741 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    10801 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    10861 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    10921 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    10981 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    11041 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    11101 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    11161 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
    11221 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    11281 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    11341 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    11401 gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc
    11461 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
    11521 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
    11581 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
    11641 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
    11701 agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc
    11761 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
    11821 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
    11881 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
    11941 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
    12001 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
    12061 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
    12121 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
    12181 ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct tttctacagt
    12241 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
    12301 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
    12361 ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct
    12421 gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag
    12481 cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac
    12541 ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt
    12601 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
    12661 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actgaattaa
    12721 cgccgaatta attcggggga tctggatttt agtactggat tttggtttta ggaattagaa
    12781 attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc
    12841 aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact actcacacat
    12901 tattatggag aaactcgagc ttgtcgatcg acagatccgg tcggcatcta ctctatttct
    12961 ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc
    13021 catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc
    13081 cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt
    13141 caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg
    13201 gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag
    13261 ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg
    13321 cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc
    13381 cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct
    13441 catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat
    13501 acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc
    13561 cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat
    13621 ccatagcctc cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca
    13681 acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgcta aactccccaa
    13741 tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat
    13801 ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat
    13861 cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt
    13921 cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat
    13981 ctcattgccc cccgggatct gcgaaagctc gagagagata gatttgtaga gagagactgg
    14041 tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga aggtcttgcg
    14101 aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt
    14161 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
    14221 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
    14281 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
    14341 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
    14401 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
    14461 ccaccatgtt atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
    14521 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
    14581 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
    14641 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
    14701 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
    14761 tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct agccaatacg
    14821 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    14881 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
    14941 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
    15001 acaatttcac acaggaaaca gctatgacca tgattacgcc a
//
