LOCUS       40924_36738        3740 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial plasmid with p15A origin and lacI gene for regulating 
            expression from pQE vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     This plasmid can be co-transformed into E. coli with expression 
            vectors such as pQE-30.
FEATURES             Location/Qualifiers
     source          1..3740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             357..1148
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(1532..2611)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(2612..2689)
                     /label=lacI promoter
     rep_origin      complement(3065..3609)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 aagcttcacg ctgccgcaag cactcagggc gcaagggctg ctaaaggaag cggaacacgt
       61 agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct
      121 ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc
      181 gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc
      241 cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg ccgccaagga
      301 tctgatggcg caggggatca agatctgatc aagagacagg atgacggtcg tttcgcatgc
      361 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
      421 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
      481 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
      541 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
      601 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
      661 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
      721 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
      781 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
      841 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
      901 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
      961 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     1021 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     1081 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     1141 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     1201 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     1261 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     1321 ccccgggctc gatcccctcg cgagttggtt cagctgctgc ctgaggctgg acgacctcgc
     1381 ggagttctac cggcagtgca aatccgtcgg catccaggaa accagcagcg gctatccgcg
     1441 catccatgcc cccgaactgc aggagtgggg aggcacgatg gccgctttgg tcgacaattc
     1501 gcgctaactt acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1561 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1621 ccagggtggt ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct
     1681 ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct
     1741 gtttgatggt ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca
     1801 ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca
     1861 gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt
     1921 gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct
     1981 gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag
     2041 aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca
     2101 cgcccagtcg cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag
     2161 agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct
     2221 ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca
     2281 ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac
     2341 ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca
     2401 gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc
     2461 ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag
     2521 aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact
     2581 ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg
     2641 ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtca acgtaaatgc
     2701 atgccgcttc gccttcgcgc gcgaattgtc gaccctgtcc ctcctgttca gctactgacg
     2761 gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctagcggagt gtatactggc
     2821 ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag
     2881 gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact
     2941 gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga
     3001 gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc
     3061 cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt
     3121 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tcccctggcg gctccctcgt
     3181 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
     3241 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
     3301 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt
     3361 ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag
     3421 gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac
     3481 tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa
     3541 aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa
     3601 cgatctcaag aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt
     3661 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgtt aattctcatg
     3721 tttgacagct tatcatcgat
//
