LOCUS       40924_36448        9769 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pRE1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Toepfer R, Filsak E, Hausmann L.
  TITLE     Binary vector for agrobacterium-mediated plant transformation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9769)
  AUTHORS   Toepfer R, Filsak E, Hausmann L.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2003) Institute for Grapevine Breeding 
            Geilweilerhof, Siebeldingen 76833, Germany
REFERENCE   3  (bases 1 to 9769)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9769)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-2003) Institute for Grapevine Breeding Geilweilerhof, 
            Siebeldingen 76833, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9769
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(333..1133)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="MGAWIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(1148..1492)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    complement(2288..2312)
                     /label=LB T-DNA repeat
                     /note="left border repeat from octopine Ach5 T-DNA"
     CDS             complement(2873..3661)
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             5565..6191
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6623..7693
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7762..7956
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8300..8440
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8626..9214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(9523..9547)
                     /label=RB T-DNA repeat
                     /note="right border repeat from octopine Ach5 T-DNA"
     misc_feature    complement(join(9766..9769,1..102))
                     /label=MCS
                     /note="pBluescript multiple cloning site"
ORIGIN
        1 gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcct gcagcccggg
       61 ggatccacta gttctagagc ggccgccacc gcggtggagc tcgctgcatg caggtcactg
      121 gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca
      181 tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataagaac cctaattccc
      241 ttatctggga actactcaca cattattctg gagaaaaata gagagagata gatttgtaga
      301 gagagactgg tgatttttgc gggtcccgct cagaagaact cgtcaagaag gcgatagaag
      361 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
      421 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
      481 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
      541 ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc
      601 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
      661 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
      721 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
      781 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
      841 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
      901 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca
      961 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     1021 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     1081 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tccaagctcc catggtggcc
     1141 actcgaggtc ctctccaaat gaaatgaact tccttatata gaggaagggt cttgcgaagg
     1201 atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt
     1261 tgaagacgtg gttggaacgt cttctttttc cacgatgttc ctcgtgggtg ggggtccatc
     1321 tttgggacca ctgtcggtag aggcatcttg aacgatagcc tttcctttat cgcaatgatg
     1381 gcatttgtag aagccatctt ccttttctac tgtcctttcg atgaagtgac agatagctgg
     1441 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
     1501 ctggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
     1561 catgtttgca tgcatgcatg catgcaagct tggtaccaga tcttataatt aaatggcctt
     1621 cgctgcccat attattggta actcaacagc atcaatcacg ggatttttct cgaattaatt
     1681 gcgtcgaatc tcagcatcga aatattcgcc tttttcgtcc attagactat ctattgtgat
     1741 ggtggattta tcacaaatgg gacccgccgc cgacagaggt gtgatgttag gccaggactt
     1801 tgaaaatttg cgcaactatc gtatagtggc cgacaaattg acgccgagtt gacagactgc
     1861 ctagcatttg agtgaattat gtaaggtaat gggctacact gaattggtag ctcaaactgt
     1921 cagtatttat gtatatgagt gtatattttc gcataatctc agaccaatct gaagatgaaa
     1981 tgggtatctg ggaatggcga aatcaaggca tcgatcgtga agtttctcat ctaagccccc
     2041 atttggacgt gaatgtagac acgtcgaaat aaagatttcc gaattagaat aatttgttta
     2101 ttgctttcgc ctataaatac gacggatcgt aatttgtcgt tttatcaaaa tgtactttca
     2161 ttttataata acgctgcgga catctacatt tttgaattga aaaaaaattg gtaattactc
     2221 tttctttttc tccatattga ccatcatact cattgctgat ccatgtagat ttcccggaca
     2281 tgaagccatt tacaattgaa tatatcctgc cgccgctgcc gctttgcacc cggtggagct
     2341 tgcatgttgg tttctacgca gaactgagcc ggttaggcag ataatttcca ttgagaactg
     2401 agccatgtgc accttccccc caacacggtg agcgacgggg caacggagtg atccacatgg
     2461 gacttttaaa catcatccgt cggatggcgt tgcgagagaa gcagtcgatc cgtgagatca
     2521 gccgacgcac cgggcaggcg cgcaacacga tcgcaaagta tttgaacgca ggtacaatcg
     2581 agccgacgtt cacggtaccg gaacgaccaa gcaagctagc ttagtaaagc cctcgctaga
     2641 ttttaatgcg gatgttgcga ttacttcgcc aactattgcg ataacaagaa aaagccagcc
     2701 tttcatgata tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa
     2761 gcagacttga cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcttg
     2821 agttaagccg cgccgcgaag cggcgtcggc ttgaacgaat tgttagacat tatttgccga
     2881 ctaccttggt gatctcgcct ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg
     2941 aggccaagcg atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct
     3001 gatactgggc cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattt
     3061 tgccggttac tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc
     3121 cagcccagtc gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat
     3181 cctgttcagg aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat
     3241 gttctcttgc ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga
     3301 tacctgcaag aatgtcattg cgctgccatt ctccaaattg cagttcgcgt ttagctggat
     3361 aacgccacgg aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct
     3421 cgctctctcc aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg
     3481 tttcatcaag ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc
     3541 cgccatccac tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct
     3601 cgatgacgcc aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca
     3661 tgatgtttaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctccata
     3721 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact
     3781 gtaccccaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     3841 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagct
     3901 tacgaaccga acaggcttat gtccactggg ttcgtgcctt catccgtttc cacggtgtgc
     3961 gtcacccggc aaccttgggc agcagcgaag tcgaggcatt tctgtcctgg ctggcgaacg
     4021 agcgcaaggt ttcggtctcc acgcatcgtc aggcattggc ggccttgctg ttcttctacg
     4081 gcaaggtgct gtgcacggat ctgccctggc ttcaggagat cggaagacct cggccgtcgc
     4141 ggcgcttgcc ggtggtgctg accccggatg aagtggttcg catcctcggt tttctggaag
     4201 gcgagcatcg tttgttcgcc cagcttctgt atggaacggg catgcggatc agtgagggtt
     4261 tgcaactgcg ggtcaaggat ctggatttcg atcacggcac gatcatcgtg cgggagggca
     4321 agggctccaa ggatcgggcc ttgatgttac ccgagagctt ggcacccagc ctgcgcgagc
     4381 aggatcgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
     4441 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
     4501 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
     4561 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
     4621 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
     4681 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
     4741 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     4801 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     4861 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     4921 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     4981 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     5041 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     5101 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     5161 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     5221 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     5281 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     5341 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     5401 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     5461 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     5521 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     5581 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     5641 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     5701 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     5761 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     5821 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     5881 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     5941 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     6001 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     6061 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     6121 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     6181 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     6241 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     6301 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     6361 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     6421 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     6481 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     6541 cgggcggttg gccaggcgta agcggctggg ttgtctgccg gccctgcaat ggcactggaa
     6601 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     6661 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     6721 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     6781 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     6841 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     6901 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     6961 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     7021 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     7081 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     7141 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     7201 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     7261 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     7321 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     7381 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     7441 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     7501 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     7561 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     7621 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     7681 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     7741 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     7801 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     7861 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     7921 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     7981 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     8041 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     8101 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     8161 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8221 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8281 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     8341 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     8401 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     8461 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     8521 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     8581 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     8641 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8701 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8761 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8821 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8881 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8941 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     9001 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     9061 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     9121 tgatagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     9181 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat ccggaaaacg caagcgcaaa
     9241 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg
     9301 gacagcaagc gaaccggaat tgccagattc gaagctcggt cccgtgggtg ttctgtcgtc
     9361 tcgttgtaca acgaaatcca ttcccattcc gcgctcaaga tggcttcccc tcggcagttc
     9421 atcagggcta aatcaatcta gccgacttgt ccggtgaaat gggctgcact ccaacagaaa
     9481 caatcaaaca aacatacaca gcgacttatt cacacgagct caaattacaa cggtatatat
     9541 cctgccagtc agcatcatca caccaaaagt taggcccgaa tagtttgaaa ttagaaagct
     9601 cgcaattgag gtctacaggc caaattcgct cttagccgta caatattact caccggtgcg
     9661 atgcccccca tcgtaggtga aggtggaaat taatgatcca tcttgagacc acaggcccac
     9721 aacagctacc agtttcctca agggtccacc aaaaacgtaa gcgcttacg
//
