LOCUS       Exported                4279 bp DNA     circular SYN 29-AUG-2024
DEFINITION  Ribosome display vector pRDV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Binz HK, Amstutz P, Kohl A, Stumpp MT, Briand C, Forrer P, Grutter 
            MG, Pluckthun A.
  TITLE     High-affinity binders selected from designed ankyrin repeat protein 
            libraries
  JOURNAL   Nat. Biotechnol. 22 (5), 575-582 (2004)
  PUBMED    15097997
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Amstutz P, Zahnd C, Binz KH, Stumpp MT, Forrer P, Pluckthun A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-JUN-2003) Biochemistry, University of Zurich, 
            Winterthurerstrasse 190, Zurich 8057, Switzerland
REFERENCE   3  (bases 1 to 4279)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4279)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4279)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol."; date: "2004"; volume: "22"; issue: "5"; pages: 
            "575-582"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-JUN-2003) Biochemistry, University of Zurich,
            Winterthurerstrasse 190, Zurich 8057, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..37
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     stem_loop       36..56
     protein_bind    60..76
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             87..109
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             123..146
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             156..941
                     /label=bla(M)
                     /note="beta-lactamase lacking the signal sequence"
     misc_feature    975..1862
                     /label=tolA spacer
                     /note="tolA spacer"
     terminator      1930..1977
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2014..2469
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2495..2599
                     /label=AmpR promoter
     CDS             2600..3457
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3631..4219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acccgacacc atcgaaatta atacgactca ctatagggag accacaacgg tttcccgaat
       61 tgtgagcgga taacaataga aataattttg tttaacttta agaaggagat atatccatgg
      121 cggactacaa agatgacgat gacaaaggat cccacccaga aacgctggtg aaagtaaaag
      181 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      241 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
      301 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
      361 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
      421 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
      481 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
      541 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
      601 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
      661 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
      721 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
      781 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
      841 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
      901 gacagatcgc tgagataggt gcctcactga ttaagcattg gggaaagctt tatatggcct
      961 cgggggccga attcggatct ggtggccaga agcaagctga agaggcggca gcgaaagcgg
     1021 cggcagatgc taaagcgaag gccgaagcag atgctaaagc tgcggaagaa gcagcgaaga
     1081 aagcggctgc agacgcaaag aaaaaagcag aagcagaagc cgccaaagcc gcagccgaag
     1141 cgcagaaaaa agccgaggca gccgctgcgg cactgaagaa gaaagcggaa gcggcagaag
     1201 cagctgcagc tgaagcaaga aagaaagcgg caactgaagc tgctgaaaaa gccaaagcag
     1261 aagctgagaa gaaagcggct gctgaaaagg ctgcagctga taagaaagcg gcagcagaga
     1321 aagctgcagc cgacaaaaaa gcagcagaaa aagcggctgc tgaaaaggca gcagctgata
     1381 agaaagcagc ggcagaaaaa gccgccgcag acaaaaaagc ggcagcggca aaagctgcag
     1441 ctgaaaaagc cgctgcagca aaagcggccg cagaggcaga tgatattttc ggtgagctaa
     1501 gctctggtaa gaatgcaccg aaaacggggg gaggggcgaa agggaacaat gcttcgcctg
     1561 ccgggagtgg taatactaaa aacaatggcg catcaggggc cgatatcaat aactatgccg
     1621 ggcagattaa atctgctatc gaaagtaagt tctatgacgc atcgtcctat gcaggcaaaa
     1681 cctgtacgct gcgcataaaa ctggcacccg atggtatgtt actggatatc aaacctgaag
     1741 gtggcgatcc cgcactttgt caggctgcgt tggcagcagc taaacttgcg aagatcccga
     1801 aaccaccaag ccaggcagta tatgaagtgt tcaaaaacgc gccattggac ttcaaaccgt
     1861 agtagagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     1921 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     1981 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     2041 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     2101 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     2161 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     2221 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     2281 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     2341 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     2401 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     2461 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2521 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2581 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     2641 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     2701 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     2761 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     2821 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     2881 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     2941 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     3001 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3061 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3121 cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3181 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3241 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     3301 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     3361 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     3421 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     3481 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3541 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3601 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3661 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3721 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3781 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3841 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3901 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3961 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4021 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4081 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4141 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4201 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4261 ctggcctttt gctcacatg
//
