LOCUS       40924_36433       14856 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pRD29A-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14856)
  AUTHORS   Chen W-P.
  TITLE     Cre-lox recombination-based plant expression vector with 
            stress-inducible promoter
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14856)
  AUTHORS   Chen W-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAY-2005) Department of Horticultural Science, 
            Laboratory of Plant Hardiness, University of Minnesota, 1970 Folwell
            Avenue, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 14856)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14856)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-MAY-2005) Department of Horticultural Science, Laboratory of 
            Plant Hardiness, University of Minnesota, 1970 Folwell Avenue, St. 
            Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(826..850)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(1011..1211)
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti
                     plasmid gene 7"
     primer_bind     complement(1244..1260)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3060..3648
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3936..3957
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3972..4002
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4010..4026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4034..4050
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4071..4089
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      4117..5065
                     /label=rd29A 1kb promoter
                     /note="rd29A 1kb promoter"
                     /regulatory_class="promoter"
     protein_bind    complement(5072..5105)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5108..5824
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      5898..6150
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6172..6188)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6221..6239)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6249..6265)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6407..6835
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        complement(6878..7258)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     CDS             7363..7911
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     primer_bind     complement(8178..8194)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    8628..8758
                     /label=Agrobacterium tumefaciens Ti plasmid left border
                     /note="Agrobacterium tumefaciens Ti plasmid left border"
     misc_feature    complement(8812..8836)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10435..11580)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(11882..12673)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     mobile_element  12865..13632
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      complement(14325..14856)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 agaggaagcg aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg
       61 catggatgcg cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc
      121 cgatcagaaa tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg
      181 ccagcatggc ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa
      241 gcgccggctg ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc
      301 cgacctcgtt caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca
      361 tgcttgacac tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa
      421 tccgcgcgtt caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat
      481 acccctgatc gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat
      541 gccggtgctg ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta
      601 tggcattctg ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc
      661 gctgtcggat cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc
      721 tggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc
      781 ccttttaaat atccgattat tctaataaac gctcttttct cttaggttta cccgccaata
      841 tatcctgtca aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc
      901 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
      961 tttggaactg acagaaccgc aacgttgaag gagccactca gccaattccc atcttgaaag
     1021 aaatatagtt taaatattta ttgataaaat aacaagtcag gtattatagt ccaagcaaaa
     1081 acataaattt attgatgcaa gtttaaattc agaaatattt caataactga ttatatcagc
     1141 tggtacattg ccgtagatga aagactgagt gcgatattat gtgtaataca taaattgatg
     1201 atatagctag cttagctcat cgggggatcc gatatcttaa gatcgatacc gtcgacctcg
     1261 aattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     1321 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     1381 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     1441 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     1501 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     1561 tattctcaga atgacttggt tgagtccaaa gctagagagc tagtttcaag gatgttaaag
     1621 ttgagatcca tttttattcc caggatccac gcaacgataa cactcaatgg gaccacgaac
     1681 aaagaaatct gagttgcaga gcccaacgca acccctagag atatgtcgag cttgttcttg
     1741 aatgcgaaaa tgatggctcc agcatgctca gccgcattcc caacaatggg aagcaatatg
     1801 atgcttatga aactcactga tagtccccat gagtccgatg catcctcgat cgtgtccaca
     1861 acatactctg atagcaatgc gatgactatt gtcatcccaa cgagccaagc aaatccgctc
     1921 cagaatccta tcactggagt ttcttcaaca ctaacctcat catcatatgc atcatcatca
     1981 tcctgttgtg cctcaaacaa ttggcggtga gtccagagct ggaagatgag ataagcaatg
     2041 taagcaataa gcataactat gctgcttgtc cgcgacagag tgagcgacat tttgttaatc
     2101 atagaggtcg atacttcgcc ggttgctgca tattttaaca ataatggcag caaatgacac
     2161 aacaggccca taagcagcaa gaagaagttc acatcggctt gtttccggtc gaaccgctgc
     2221 tcgcggcgga tattggcgat accaccaaag aagagggaag tgccaagaac caagagaagg
     2281 tttgagagaa ttgaacccaa tagagagtat ttcaccactg ccactttgtt attggactca
     2341 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     2401 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     2461 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     2521 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     2581 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     2641 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     2701 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     2761 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     2821 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     2881 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     2941 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     3001 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     3061 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     3121 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3181 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3241 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3301 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3361 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3421 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3481 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3541 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3601 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3661 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3721 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3781 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     3841 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     3901 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     3961 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     4021 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     4081 cactaaaggg aacaaaagct gggtaccggg ccccccctcg aggagccata gatgcaattc
     4141 aatcaaactg aaatttctgc aagaatctca aacacggaga tctcaaagtt tgaaagaaaa
     4201 tttatttctt cgactcaaaa caaacttacg aaatttaggt agaacttata tacattatat
     4261 tgtaattttt tgtaacaaaa tgtttttatt attattatag aattttactg gttaaattaa
     4321 aaatgaatag aaaaggtgaa ttaagaggag agaggaggta aacattttct tctatttttt
     4381 catattttca ggataaatta ttgtaaaagt ttacaagatt tccatttgac tagtgtaaat
     4441 gaggaatatt ctctagtaag atcattattt catctacttc ttttatcttc taccagtaga
     4501 ggaataaaca atatttagct cctttgtaaa tacaaattaa ttttcgttct tgacatcatt
     4561 caattttaat tttacgtata aaataaaaga tcatacctat tagaacgatt aaggagaaat
     4621 acaattcgaa tgagaaggat gtgccgtttg ttataataaa cagccacacg acgtaaacgt
     4681 aaaatgacca catgatgggc caatagacat ggaccgacta ctaataatag taagttacat
     4741 tttaggatgg aataaatatc ataccgacat cagtttgaaa gaaaagggaa aaaaagaaaa
     4801 aataaataaa agatatacta ccgacatgag ttccaaaaag caaaaaaaaa gatcaagccg
     4861 acacagacac gcgtagagag caaaatgact ttgacgtcac accacgaaaa cagacgcttc
     4921 atacgtgtcc ctttatctct ctcagtctct ctataaactt agtgagaccc tcctctgttt
     4981 tactcacaaa tatgcaaact agaaaacaat catcaggaat aaagggtttg attacttcta
     5041 ttggaaagaa aaaaatcttt ggaaactcga gataacttcg tatagcatac attatacgaa
     5101 gttatttatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     5161 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     5221 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     5281 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     5341 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     5401 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     5461 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     5521 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     5581 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     5641 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     5701 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     5761 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     5821 caagtaaatc aacaactctc ctggcgcacc atcgtcggct acagcctcgg gaattgctac
     5881 cgagctcgaa tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc
     5941 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     6001 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     6061 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     6121 cgcgcgcggt gtcatctatg ttactagatc gggaattcct gcagcccggg ggatccacta
     6181 gttctagagc ggccgccacc gcggtggagc tccaattcgc cctatagtga gtcgtattac
     6241 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6301 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6361 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     6421 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6481 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6541 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6601 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6661 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6721 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6781 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     6841 aaaatgggtg atcagtttaa acaagcttga attaattcga tttggtgtat cgagattggt
     6901 tatgaaattc agatgctagt gtaatgtatt ggtaatttgg gaagatataa taggaagcaa
     6961 ggctatttat ccatttctga aaaggcgaaa tggcgtcacc gcgagcgtca cgcgcattcc
     7021 gttcttgctg taaagcgttg tttggtacac ttttgactag cgaggcttgg cgtgtcagcg
     7081 tatctattca aaagtcgtta atggctgcgg atcaagaaaa agttggaata gaaacagaat
     7141 acccgcgaaa ttcaggcccg gttgccatgt cctacacgcc gaaataaacg accaaattag
     7201 tagaaaaata aaaactgact cggatactta cgtcacgtct tgcgcactga tttgaaaaat
     7261 ctcaatataa acaaagacgg ccacaagaaa aaaccaaaac accgatattc attaatctta
     7321 tctagtttct caaaaaaatt catatcttcc acacgtggat ccatgagccc agaacgacgc
     7381 ccggccgaca tccgccgtgc caccgaggcg gacatgccgg cggtctgcac catcgtcaac
     7441 cactacatcg agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg
     7501 acggacgacc tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga ggtggacggc
     7561 gaggtcgccg gcatcgccta cgcgggcccc tggaaggcac gcaacgccta cgactggacg
     7621 gccgagtcga ccgtgtacgt ctccccccgc caccagcgga cgggactggg ctccacgctc
     7681 tacacccacc tgctgaagtc cctggaggca cagggcttca agagcgtggt cgctgtcatc
     7741 gggctgccca acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc cccccgcggc
     7801 atgctgcggg cggccggctt caagcacggg aactggcatg acgtgggttt ctggcagctg
     7861 gacttcagcc tgccggtacc gccccgtccg gtcctgcccg tcaccgagat ctgagatcac
     7921 gcgttctagg atcctctaga gtcgacctgc aggcatgccc gctgaaatca ccagtctctc
     7981 tctacaaatc tatctctctc tataataatg tgtgagtagt tcccagataa gggaattagg
     8041 gttcttatag ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta
     8101 tttgtaaaat acttcttatc aataaaattt ctaattccta aaaccaaaat cccagggtac
     8161 cgagctcgaa ttaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     8221 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     8281 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt
     8341 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     8401 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     8461 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     8521 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     8581 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
     8641 caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc
     8701 caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccacccca
     8761 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
     8821 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
     8881 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
     8941 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
     9001 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
     9061 gctgcctgtg atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc
     9121 atttctcgct taaccgtgac aggctgtcga tcttgagaac tatgccgaca taataggaaa
     9181 tcgctggata aagccgctga ggaagctgag tggcgctatt tctttagaag tgaacgttga
     9241 cgatatcaac tcccctatcc attgctcacc gaatggtaca ggtcggggac ccgaagttcc
     9301 gactgtcggc ctgatgcatc cccggctgat cgaccccaga tctggggctg agaaagccca
     9361 gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta
     9421 aacccgctcc gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg
     9481 ggattggcgg atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt
     9541 gccaggcggt aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc
     9601 cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg
     9661 cgtttggtgt caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg
     9721 ccatcaatcg tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct
     9781 gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca
     9841 agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc
     9901 ccgttggaat ctgtcggacg atcatcacga gcaataaacc cgccggcaac gcccgcagca
     9961 gcataccggc gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg
    10021 ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc
    10081 cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg
    10141 gctttttctc ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg
    10201 acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga atctgagcct
    10261 taatcacaat tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg
    10321 tcccgagcga tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc
    10381 cagtaaagcg ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt
    10441 gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc
    10501 ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca
    10561 catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc
    10621 gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta
    10681 gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt
    10741 tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc
    10801 aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc
    10861 cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt
    10921 gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac
    10981 cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt
    11041 gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt
    11101 gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc
    11161 aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc
    11221 ggcctgcttg gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt
    11281 ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg
    11341 ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag
    11401 ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg
    11461 gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc
    11521 ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat
    11581 tcaccctcct tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt
    11641 tgacccgcct ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc
    11701 gtagaccgtc tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac
    11761 cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat
    11821 gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca tctaggtact
    11881 aaaacaattc atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca
    11941 gtaagtcaaa aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga
    12001 cgcagaaggc aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc
    12061 cacttacttt gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga
    12121 caagttcctc ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa
    12181 atggagtgtc ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt
    12241 aatccaattc ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga
    12301 tggagtgaaa gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt
    12361 catcttcata ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc
    12421 agccatcatg ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata
    12481 gcatcatgtc cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca
    12541 tttttaaata taggttttca ttttctccca ccagcttata taccttagca ggagacattc
    12601 cttccgtatc ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc
    12661 tcattttagc catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga
    12721 agctaattat aacaagacga actccaattc actgttcctt gcattctaaa accttaaata
    12781 ccagaaaaca gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga
    12841 gccgattttg aaaccacaat tatgggtgat gctgccaact tactgattta gtgtatgatg
    12901 gtgtttttga ggtgctccag tggcttctgt gtctatcagc tgtccctcct gttcagctac
    12961 tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca
    13021 ctgccgtaaa acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcacca
    13081 gaaaatcatt gatatggcca tgaatggcgt tggatgccgg gcaacagccc gcattatggg
    13141 cgttggcctc aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg
    13201 cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat
    13261 gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg
    13321 gttgttgcgc acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg
    13381 ctgtcaccct ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc
    13441 ctgaagggaa agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac
    13501 ctgaatctga ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg
    13561 gtggagctgc atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt
    13621 ggagtcatta cccaattatg atagaattta caagctataa ggttattgtc ctgggtttca
    13681 agcattagtc catgcaagtt tttatgcttt gcccattcta tagatatatt gataagcgcg
    13741 ctgcctatgc cttgccccct gaaatcctta catacggcga tatcttctat ataaaagata
    13801 tattatctta tcagtattgt caatatattc aaggcaatct gcctcctcat cctcttcatc
    13861 ctcttcgtct tggtagcttt ttaaatatgg cgcttcatag agtaattctg taaaggtcca
    13921 attctcgttt tcatacctcg gtataatctt acctatcacc tcaaatggtt cgctgggttt
    13981 atcgcacccc cgaacacgag cacggcaccc gcgaccacta tgccaagaat gcccaaggta
    14041 aaaattgccg gccccgccat gaagtccgtg aatgccccga cggccgaagt gaagggcagg
    14101 ccgccaccca ggccgccgcc ctcactgccc ggcacctggt cgctgaatgt cgatgccagc
    14161 acctgcggca cgtcaatgct tccgggcgtc gcgctcgggc tgatcgccca tcccgttact
    14221 gccccgatcc cggcaatggc aaggactgcc agcgctgcca tttttggggt gaggccgttc
    14281 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt
    14341 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg
    14401 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg
    14461 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc
    14521 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg
    14581 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc
    14641 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg
    14701 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc
    14761 cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc
    14821 caagttttcc gcgaggtatc cacaacgccg gcggcc
//
