LOCUS       62056_18770        5055 bp DNA     circular SYN 09-FEB-2019
DEFINITION  Expression vector pRD2, complete sequence.
ACCESSION   MH298521
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5055)
  AUTHORS   Knopp M.
  TITLE     De novo emergence of peptides conferring antibiotic resistance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5055)
  AUTHORS   Knopp M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2018) Medical Biochemistry and Microbiology, 
            Uppsala University, Husargatan 3, Uppsala 75123, Sweden
REFERENCE   3  (bases 1 to 5055)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-MAY-2018) Medical Biochemistry and Microbiology, Uppsala 
            University, Husargatan 3, Uppsala 75123, Sweden"
FEATURES             Location/Qualifiers
     source          1..5055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      22..67
                     /label=lux terminator
                     /note="lux terminator"
                     /regulatory_class="terminator"
     CDS             complement(80..937)
                     /label=AmpR
                     /note="beta-lactamase"
     regulatory      complement(958..997)
                     /label=predicted promoter
                     /note="predicted promoter"
                     /regulatory_class="promoter"
     regulatory      complement(977..1016)
                     /label=predicted promoter
                     /note="predicted promoter"
                     /regulatory_class="promoter"
     terminator      1037..1064
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     regulatory      1091..1149
                     /label=T21 terminator
                     /note="T21 terminator"
                     /regulatory_class="terminator"
     protein_bind    1179..1195
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    1232..1253
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      1254..1265
                     /regulatory_class="ribosome_binding_site"
     terminator      complement(1329..1360)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     regulatory      1408..1448
                     /label=terminator
                     /note="terminator"
                     /regulatory_class="terminator"
     terminator      complement(1469..1503)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1570..2988)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     regulatory      complement(3012..3046)
                     /label=PsacB-J23113
                     /note="PsacB-J23113"
                     /regulatory_class="promoter"
     rep_origin      3064..3608
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        3886..3963
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3964..5043
                     /label=lacI
                     /note="lac repressor"
ORIGIN
        1 cacacaacca caccacacca caaataataa aaaagccgga ttaataatct ggctttttat
       61 attctctgtc gacttattac caatgcttaa tcagggaggc acctatctca gcgatctggc
      121 gattacgttc gtccatagtt gcctggctcc ccgtcgtgta gataactacg atacgggagg
      181 gcttaccatc tggccccagg gctgcaataa taccgcggga cccacgctca ccggctccag
      241 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
      301 tatccgcctc catccagtct attaattgtt gccgggaagc gagagtaaga agttcgccag
      361 ttaagagttt gcgcaacgtt gttgccatag ctacaggcat cgtggtgtca cgctcgtcgt
      421 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatcaccca
      481 tgttgtgcaa aaaagcggta agctccttcg gtcctccgat cgttgtcaga agtaagttgg
      541 ccgccgtgtt atcactcatg gttatggcag cgctgcataa ttcgcgtact gtcatgccgt
      601 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
      661 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacagagca
      721 gaactttaaa agtgctcatc attgggaaac gttcttcggg gcgaaaactc tcaagaatct
      781 taccgctgtt gaggtccagt tcgatgtaac ccacacgtgc acccaactga tcttcggcat
      841 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
      901 agggaataag ggcgacacgg aaatgttgaa tactcatatt cttccttttt caatattatt
      961 gaagcattta tcagggttat tgtctcatga gcggatagat atttgaatgt atttagagct
     1021 caaatgtaat cacactggct caccttcggg tgggcctttc tgcgtttata tgccacctga
     1081 cgtctaagaa ataagctgcc gttagtgact cttaagttgc aacggtggct ttttttattt
     1141 gggtcagtct tagttagtta gctagttcta gcatgcaatt gtgagcggat aacaattgac
     1201 attgtgagcg gataacaaga tactgagcac agaattcgcg gccgcttcta gagaaagagg
     1261 agaaaggatc catgtaataa tactagtctg caggagtcac taagggttag ttagttagat
     1321 tagcagaaag tcaaaagcct ccggtcggag gcttttgact aaaacttccc ttggggttat
     1381 cattggggct cactcaaagg cggtaatcag ataaaaaaaa tccttagctt tcgctaagga
     1441 tgatttctgc tagagttctc gaccaccaat aaaaaacgcc cggcggcaac cgagcgttct
     1501 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcca
     1561 aatttattat ttgttaactg ttaattgtcc ttgttcaagg atgctgtctt tgacaacaga
     1621 tgttttcttg cctttgatgt tcagcaggaa gcttggcgca aacgttgatt gtttgtctgc
     1681 gtagaatcct ctgtttgtca tatagcttgt aatcacgaca ttgtttcctt tcgcttgagg
     1741 tacagcgaag tgtgagtaag taaaggttac atcgttagga tcaagatcca tttttaacac
     1801 aaggccagtt ttgttcagcg gcttgtatgg gccagttaaa gaattagaaa cataaccaag
     1861 catgtaaata tcgttagacg taatgccgtc aatcgtcatt tttgatccgc gggagtcagt
     1921 gaacaggtac catttgccgt tcattttaaa gacgttcgcg cgttcaattt catctgttac
     1981 tgtgttagat gcaatcagcg gtttcatcac ttttttcagt gtgtaatcat cgtttagctc
     2041 aatcataccg agagcgccgt ttgctaactc agccgtgcgt tttttatcgc tttgcagaag
     2101 tttttgactt tcttgacgga agaatgatgt gcttttgcca tagtatgctt tgttaaataa
     2161 agattcttcg ccttggtagc catcttcagt tccagtgttt gcttcaaata ctaagtattt
     2221 gtggccttta tcttctacgt agtgaggatc tctcagcgta tggttgtcgc ctgagctgta
     2281 gttgccttca tcgatgaact gctgtacatt ttgatacgtt tttccgtcac cgtcaaagat
     2341 tgatttataa tcctctacac cgttgatgtt caaagagctg tctgatgctg atacgttaac
     2401 ttgtgcagtt gtcagtgttt gtttgccgta atgtttaccg gagaaatcag tgtagaataa
     2461 acggattttt ccgtcagatg taaatgtggc tgaacctgac cattcttgtg tttggtcttt
     2521 taggatagaa tcatttgcat cgaatttgtc gctgtcttta aagacgcggc cagcgttttt
     2581 ccagctgtca atagaagttt cgccgacttt ttgatagaac atgtaaatcg atgtgtcatc
     2641 cgcattttta ggatctccgg ctaatgcaaa gacgatgtgg tagccgtgat agtttgcgac
     2701 agtgccgtca gcgttttgta atggccagct gtcccaaacg tccaggcctt ttgcagaaga
     2761 gatattttta attgtggacg aatcgaactc aggaacttga tatttttcat ttttttgctg
     2821 ttcagggatt tgcagcatat catggcgtgt aatatgggaa atgccgtatg tttccttata
     2881 tggcttttgg ttcgtttctt tcgcaaacgc ttgagttgcg cctcctgcca gcagtgcggt
     2941 agtaaaggtt aatactgttg cttgttttgc aaactttttg atgttcatcg ttcatgtctc
     3001 ctctactcga ggctagcact atccctagga ctgagctagc catcagttaa taagatgatc
     3061 ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt
     3121 gcagggcggt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
     3181 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
     3241 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
     3301 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
     3361 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
     3421 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     3481 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg
     3541 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
     3601 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
     3661 ttccaggaaa tctccgccca gttcgtaagc catttccgct cgccgcagta gaacgaccga
     3721 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
     3781 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
     3841 ccaacatagt aagccagtat acactacgct agctgcattt acgttgacac tatcgaatgg
     3901 tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggaggtg
     3961 aatatgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc
     4021 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa
     4081 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa
     4141 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt
     4201 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta
     4261 gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc
     4321 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc
     4381 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt
     4441 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac
     4501 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct
     4561 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac
     4621 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc
     4681 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag
     4741 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc
     4801 tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc
     4861 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg
     4921 cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc
     4981 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     5041 cagtaataag tcgag
//
