LOCUS       V003824                 4566 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003824
VERSION     V003824
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Guo L-X., Chen J-M., Wang Z-L., Sun C-Y., Li L, Li J-M., Song C-P.
  TITLE     Establishment of a Taqman real time RT-PCR assay for the detection
            of Nipah virus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Guo L-X., Chen J-M., Wang Z-L., Sun C-Y., Li L, Li J-M., Song C-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-APR-2006) Chinese Center for Animal Health and
            Epidemiology, Nanjing Road 369, Qingdao, Shandong 266032, China
REFERENCE   3  (bases 1 to 4566)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4566)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-APR-2006) Chinese Center for Animal Health and Epidemiology,
            Nanjing Road 369, Qingdao, Shandong 266032, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             23..1618
                     /gene="N"
                     /label="Nucleoprotein"
                     /note="Nucleoprotein from Nipah virus. Accession#: Q9IK92"
     terminator      1728..1775
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      1872..2327
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2408..2512
                     /label="AmpR promoter"
     misc_feature    2697..3075
                     /label="ISS"
                     /note="immunostimulatory sequence from the AmpR gene;
                     contains unmethylated CpG dinucleotides in the context of
                     5'-AACGTT-3' (Sato et al., 1996)"
     rep_origin      3544..4132
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        4387..4405
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             4436..4458
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4478..4495
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             4499..4531
                     /label="T7 tag (gene 10 leader)"
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             4535..4558
                     /label="Xpress(TM) tag"
                     /note="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
ORIGIN
        1 gatcctcaag aaatatatca tcatgagtga tatctttgaa gaggcggcta gttttaggag
       61 ttatcaatct aagttaggga gagatgggag ggctagtgca gcaactgcta ctttgacaac
      121 caagataagg atatttgtac cagctactaa tagtccagag ctcagatggg aactaacatt
      181 gtttgcactt gatgtgatta gatctccgag tgctgccgag tcaatgaaag ttggagctgc
      241 tttcacactc atctctatgt attcagagag acccggggct ctcattagaa gtctcctcaa
      301 tgacccagac attgaagctg taataataga tgttggatca atggtcaacg gaataccagt
      361 aatggagagg agaggagaca aggctcagga ggagatggaa ggcttgatga gaatcctcaa
      421 aactgctcga gacagcagca agggaaaaac accttttgtt gacagccgag cttacggcct
      481 acggataaca gacatgagca ccctggtctc tgcagttatc accatcgagg cccagatctg
      541 gatactgatc gctaaagcag ttacagctcc cgacactgcc gaggaaagtg aaactagaag
      601 atgggctaaa tacgtccaac aaaagagagt caatccgttc tttgctctaa ctcagcaatg
      661 gctaacagaa atgaggaatc tgctctccca gagtctatca gtaaggaagt tcatggttga
      721 gatcctcata gaagtcaaga aaggaggatc tgctaaaggc agagcagtag aaataatctc
      781 agacatcgga aactatgtcg aggaaactgg tatggcagga ttcttcgcaa ccatcagatt
      841 cgggttggag acaaggtatc cagcacttgc actcaacgaa ttccagagtg acctcaacac
      901 catcaaaagc ttgatgctac tctacagaga aattggccca agagcccctt atatggtgct
      961 tcttgaagaa tcaattcaga ctaaatttgc ccctggaggt tacccattat tgtggagctt
     1021 tgccatgggt gtggctacta ctattgacag gtctatgggg gcattgaata tcaatcgtgg
     1081 ttatcttgag cctatgtatt tcagactagg ccaaaaatca gcacgtcacc atgctggagg
     1141 aattgatcag aacatggcaa atagactggg actaagttca gatcaagttg cagaactcgc
     1201 tgctgcagtt caggaaacat cagcaggaag gcaagagagt aatgttcagg ctagagaggc
     1261 aaaatttgct gcaggaggtg tgctcattgg aggcagtgat caagatatcg atgaagggga
     1321 agaacctata gaacagagtg gcagacagtc agttaccttc aaaagggaga tgagtatttc
     1381 atcccttgct aacagtgtgc cgagcagttc tgtgagcaca tccggtggga ccagattgac
     1441 taattcatta ctaaacctca gatcaagact ggctgcaaaa gcagcaaaag aagccgcctc
     1501 atccaatgca acagatgatc cagcaatcag caacagaact caaggggaat cagagaagaa
     1561 gaataatcaa gacctcaaac ctgctcaaaa tgaccttgat ttcgtcagag ctgatgtgtg
     1621 acgtctattt ccaatattct acggtaccat ggaattcgaa gcttgatccg gctgctaaca
     1681 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     1741 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
     1801 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     1861 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     1921 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     1981 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2041 gttccgattt agagctttac ggcacctcga ccgcaaaaaa cttgatttgg gtgatggttc
     2101 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2161 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatcg cggtctattc
     2221 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2281 acaaatattt aacgcgaatt ttaacaaaat attaacgttt acaatttcgc ctgatgcggt
     2341 attttctcct tacgcatctg tgcggtattt cacaccgcat acaggtggca cttttcgggg
     2401 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2461 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2521 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2581 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2641 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2701 ttttccaatg atgagcactt ttaaagttct gctatgtgat acactattat cccgtattga
     2761 cgccgggcaa gagcaactcg gtcgcccccc gcggtattct cagaatgact tggttgagta
     2821 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2881 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2941 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3001 ggaaccggag ctgaatgaag ccataccaaa cgacgagagt gacaccacga tgcctgtagc
     3061 aatgccaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3121 acaattaata gactgaatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3181 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3241 cattgcagca ctggggccag atggtaagcg ctcccgtatc gtagttatct acacgacggg
     3301 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3361 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3421 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3481 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3541 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3601 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3661 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3721 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3781 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3841 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3901 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3961 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4021 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4081 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4141 caacgcggcc tttttacggt tcctgggctt ttgctggcct tttgctcaca tgttctttcc
     4201 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4261 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4321 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagat ctcgatcccg
     4381 cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga ataattttgt
     4441 ttaactttaa gaaggagata tacatatgcg gggttctcat catcatcatc atcatggtat
     4501 ggctagcatg actggtggac agcaaatggg tcgggatctg tacgacgatg acgataagga
     4561 tcgatg
//
