LOCUS       62056_18740        5663 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5663)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        100..131
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             164..1813
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1860..1981)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2175..2532
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2563..3354
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3382..3430
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3757..4345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4548..5405)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    5572..5663
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtaccg ctagcctcga taggtcacct ggaggtcacg gaggtcacct
       61 ggaggtcacg ggcgcgtaga tctgcagaag cttagacact agagggtata taatggaagc
      121 tcgacttcca gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaaaaa
      181 cattaagaag ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct
      241 gcacaaagcc atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca
      301 tatcgaggtg gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc
      361 tatgaagcgc tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt
      421 gcagttcttc atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa
      481 cgacatctac aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt
      541 attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat
      601 acaaaagatc atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac
      661 cttcgtgact tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt
      721 cgaccgggac aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa
      781 gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat
      841 cttcggcaac cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg
      901 cttcggcatg ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta
      961 ccgcttcgag gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct
     1021 gctggtgccc acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct
     1081 aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc
     1141 cgtggccaaa cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac
     1201 cagcgccatt ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt
     1261 gcccttcttc gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca
     1321 gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga
     1381 ggctacaaac gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg
     1441 ggacgaggac gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg
     1501 ctaccaggta gccccagccg aactggagag catcctgctg caacacccca acatcttcga
     1561 cgccggggtc gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt
     1621 gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt
     1681 tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact
     1741 gaccggcaag ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg
     1801 caagatcgcc gtgtaataat tctagagtcg gggcggccgg ccgcttcgag cagacatgat
     1861 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     1921 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     1981 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     2041 ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt ttgcgtattg
     2101 ggcgctcttc cgctgatctg cgcagcacca tggcctgaaa taacctctga aagaggaact
     2161 tggttagcta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt
     2221 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     2281 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     2341 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     2401 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     2461 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     2521 ggcttttgca aaaagctcga ttcttctgac actagcgcca ccatgatcga acaagacggc
     2581 ctccatgctg gcagtcccgc agcttgggtc gaacgcttgt tcgggtacga ctgggcccag
     2641 cagaccatcg gatgtagcga tgcggccgtg ttccgtctaa gcgctcaagg ccggcccgtg
     2701 ctgttcgtga agaccgacct gagcggcgcc ctgaacgagc ttcaagacga ggctgcccgc
     2761 ctgagctggc tggccaccac cggcgtaccc tgcgccgctg tgttggatgt tgtgaccgaa
     2821 gccggccggg actggctgct gctgggcgag gtccctggcc aggatctgct gagcagccac
     2881 cttgcccccg ctgagaaggt ttctatcatg gccgatgcaa tgcggcgcct gcacaccctg
     2941 gaccccgcta cctgcccctt cgaccaccag gctaagcatc ggatcgagcg tgctcggacc
     3001 cgcatggagg ccggcctggt ggaccaggac gacctggacg aggagcatca gggcctggcc
     3061 cccgctgaac tgttcgcccg actgaaagcc cgcatgccgg acggtgagga cctggttgtc
     3121 acacacggag atgcctgcct ccctaacatc atggtcgaga atggccgctt ctccggcttc
     3181 atcgactgcg gtcgcctagg agttgccgac cgctaccagg acatcgccct ggccacccgc
     3241 gacatcgctg aggagcttgg cggcgagtgg gccgaccgct tcttagtctt gtacggcatc
     3301 gcagctcccg acagccagcg catcgccttc taccgcttgc tcgacgagtt cttttaatga
     3361 tctagaaccg gtcatggccg caataaaata tctttatttt cattacatct gtgtgttggt
     3421 tttttgtgtg ttcgaactag atgctgtcga ccgatgccct tgagagcctt caacccagtc
     3481 agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt
     3541 atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc
     3601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     3961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4141 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     4201 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4261 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4321 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     4381 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     4441 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     4501 aacttggtct gacagcggcc gcaaatgcta aaccactgca gtggttacca gtgcttgatc
     4561 agtgaggcac cgatctcagc gatctgccta tttcgttcgt ccatagtggc ctgactcccc
     4621 gtcgtgtaga tcactacgat tcgtgagggc ttaccatcag gccccagcgc agcaatgatg
     4681 ccgcgagagc cgcgttcacc ggcccccgat ttgtcagcaa tgaaccagcc agcagggagg
     4741 gccgagcgaa gaagtggtcc tgctactttg tccgcctcca tccagtctat gagctgctgt
     4801 cgtgatgcta gagtaagaag ttcgccagtg agtagtttcc gaagagttgt ggccattgct
     4861 actggcatcg tggtatcacg ctcgtcgttc ggtatggctt cgttcaactc tggttcccag
     4921 cggtcaagcc gggtcacatg atcacccata ttatgaagaa atgcagtcag ctccttaggg
     4981 cctccgatcg ttgtcagaag taagttggcc gcggtgttgt cgctcatggt aatggcagca
     5041 ctacacaatt ctcttaccgt catgccatcc gtaagatgct tttccgtgac cggcgagtac
     5101 tcaaccaagt cgttttgtga gtagtgtata cggcgaccaa gctgctcttg cccggcgtct
     5161 atacgggaca acaccgcgcc acatagcagt actttgaaag tgctcatcat cgggaatcgt
     5221 tcttcggggc ggaaagactc aaggatcttg ccgctattga gatccagttc gatatagccc
     5281 actcttgcac ccagttgatc ttcagcatct tttactttca ccagcgtttc ggggtgtgca
     5341 aaaacaggca agcaaaatgc cgcaaagaag ggaatgagtg cgacacgaaa atgttggatg
     5401 ctcatactcg tcctttttca atattattga agcatttatc agggttacta gtacgtctct
     5461 caaggataag taagtaatat taaggtacgg gaggtattgg acaggccgca ataaaatatc
     5521 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
     5581 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
     5641 tgcaggtgcc agaacatttc tct
//
