LOCUS       40924_36373        3161 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Broad host range expression vector with a trimethoprim resistance 
            marker and with the pBBR1 replicon for Gram-negative bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Lynch MD, Gill RT.
  TITLE     Broad host range vectors for stable genomic library construction.
  JOURNAL   Biotechnol. Bioeng. 2006;94:151-8.
  PUBMED    16496398
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3161)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Bioeng."; date: "2006"; volume: "94"; pages: "151-8"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3161
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(60..96)
                     /label=soxR terminator
                     /note="bidirectional E. coli soxR transcription terminator"
     rep_origin      102..871
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             872..1531
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             1631..1864
                     /codon_start=1
                     /label=TpR
                     /note="E. coli plasmid-associated dihydrofolate reductase"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     terminator      complement(1884..1915)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     CDS             complement(1946..2821)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2848..3132
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
ORIGIN
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgcggccacc ggctggctcg
      121 cttcgctcgg cccgtggaca accctgctgg acaagctgat ggacaggctg cgcctgccca
      181 cgagcttgac cacagggatt gcccaccggc tacccagcct tcgaccacat acccaccggc
      241 tccaactgcg cggcctgcgg ccttgcccca tcaatttttt taattttctc tggggaaaag
      301 cctccggcct gcggcctgcg cgcttcgctt gccggttgga caccaagtgg aaggcgggtc
      361 aaggctcgcg cagcgaccgc gcagcggctt ggccttgacg cgcctggaac gacccaagcc
      421 tatgcgagtg ggggcagtcg aaggcgaagc ccgcccgcct gccccccgag cctcacggcg
      481 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
      541 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
      601 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
      661 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
      721 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
      781 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
      841 ccgtgcggca ccctaccgca tggagataag catggccacg cagtccagag aaatcggcat
      901 tcaagccaag aacaagcccg gtcactgggt gcaaacggaa cgcaaagcgc atgaggcgtg
      961 ggccgggctt attgcgagga aacccacggc ggcaatgctg ctgcatcacc tcgtggcgca
     1021 gatgggccac cagaacgccg tggtggtcag ccagaagaca ctttccaagc tcatcggacg
     1081 ttctttgcgg acggtccaat acgcagtcaa ggacttggtg gccgagcgct ggatctccgt
     1141 cgtgaagctc aacggccccg gcaccgtgtc ggcctacgtg gtcaatgacc gcgtggcgtg
     1201 gggccagccc cgcgaccagt tgcgcctgtc ggtgttcagt gccgccgtgg tggttgatca
     1261 cgacgaccag gacgaatcgc tgttggggca tggcgacctg cgccgcatcc cgaccctgta
     1321 tccgggcgag cagcaactac cgaccggccc cggcgaggag ccgcccagcc agcccggcat
     1381 tccgggcatg gaaccagacc tgccagcctt gaccgaaacg gaggaatggg aacggcgcgg
     1441 gcagcagcgc ctgccgatgc ccgatgagcc gtgttttctg gacgatggcg agccgttgga
     1501 gccgccgaca cgggtcacgc tgccgcgccg gtagtacgta agaggttcca actttcacca
     1561 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     1621 ggaagctaaa atgggtcaaa gtagcgatga agccaacgct cccgttgcag ggcagtttgc
     1681 gcttcccctg agtgccacct ttggcttagg ggatcgcgta cgcaagaaat ctggtgccgc
     1741 ttggcagggt caagtcgtcg gttggtattg cacaaaactc actcctgaag gctatgcggt
     1801 cgagtccgaa tcccacccag gctcagtgca aatttatcct gtggctgcac ttgaacgtgt
     1861 ggcctaagaa tggatccccc tcaagtcaaa agcctccggt cggaggcttt tgactttctg
     1921 ctatggaggt caggtatgat ttttatgaca acttgacggc tacatcattc actttttctt
     1981 cacaaccggc acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga
     2041 aatagagttg atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg
     2101 tgctcaaaag cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa
     2161 tccctaactg ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg
     2221 cgacgctggc gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct
     2281 cgcgtacccg attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca
     2341 gtaacaattg ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc
     2401 cggcgttaat gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc
     2461 gaaagaaccc cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc
     2521 gcggacgaaa gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt
     2581 gatgaatctc tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc
     2641 cctgattttt caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc
     2701 ccagcggtcg gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg
     2761 ccaccagatg ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca
     2821 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt
     2881 gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta
     2941 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct
     3001 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca
     3061 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
     3121 ctgtttctcc atacccgttt ttttgggcta gagaattcgt c
//
