LOCUS       V003826                 7949 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003826
VERSION     V003826
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Ransom EM, Ellermeier CD, Weiss DS.
  TITLE     Fluorescent protein vectors for Clostridium difficile
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   Ransom EM, Ellermeier CD, Weiss DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2015) Microbiology, The University of Iowa, 51
            Newton Rd, Iowa City, IA 52242, USA
REFERENCE   3  (bases 1 to 7949)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7949)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-AUG-2015) Microbiology, The University of Iowa, 51 Newton Rd,
            Iowa City, IA 52242, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(58..681)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     protein_bind    788..806
                     /label="tet operator"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             900..1616
                     /label="ECFP"
                     /note="enhanced CFP"
     CDS             complement(1968..2336)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(2369..2478)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(2857..3477)
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      complement(3840..4428)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5221..6858)
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label="repA"
                     /note="plasmid replication; derived from Clostridium
                     difficile"
                     /protein_id="AMA07811.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNSK
                     QLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINRYA
                     LGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVSEVT
                     NTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKKYCKD
                     VKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQVISVA
                     REWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIYSDEDTL
                     FNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITYKRKKKVK
                     NIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYTISYFVTMA
                     VIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQQRISNMIES
                     LLDTKILDVWETKINDRGFMYYTYRLNK"
     gene            complement(5221..6858)
                     /gene="repA"
                     /label="repA"
     CDS             complement(7133..7645)
                     /codon_start=1
                     /gene="orfB"
                     /product="OrfB"
                     /label="orfB"
                     /protein_id="AMA07812.1"
                     /translation="MRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNII
                     NDKLYYSNIAEFEEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNIT
                     KNGNTNYLKVKNKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSR
                     PSRVFLK"
     gene            complement(7133..7645)
                     /gene="orfB"
                     /label="orfB"
ORIGIN
        1 gctagcataa aaataagaag cctgcatttg caggcttctt atttttatgg taccttaaga
       61 cccactttca catttaagtt gtttttctaa tccgcatatg atcaattcaa ggccgaataa
      121 gaaggctggc tctgcacctt ggtgatcaaa taattcgata gcttgtcgta ataatggcgg
      181 catactatca gtagtaggtg tttccctttc ttctttagcg acttgatgct cttgatcttc
      241 caatacgcaa cctaaagtaa aatgccccac agcgctgagt gcatataatg cattctctag
      301 tgaaaaacct tgttggcata aaaaggctaa ttgattttcg agagtttcat actgtttttc
      361 tgtaggccgt gtacctaaat gtacttttgc tccatcgcga tgacttagta aagcacatct
      421 aaaactttta gcgttattac gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa
      481 gtgagtatgg tgcctatcta acatctcaat ggctaaggcg tcgagcaaag cccgcttatt
      541 ttttacatgc caatacaatg taggctgctc tacacctagc ttctgggcga gtttacgggt
      601 tgttaaacct tcgattccga cctcattaag cagctctaat gcgctgttaa tcactttact
      661 tttatctaat ctagacatca ttaattcctc ctttttgttg acattatatc attgatagag
      721 ttatttgtca aactagtttt ttatttcgat gccctggact tcatgaaaaa ctaaaaaaaa
      781 tattgacact ctatcattga tagagtataa ttaaaataag cttgatcgta gcgttaacag
      841 atctgagctc ctgcagtaaa ggagttaatt aacagctggt cgacgcatgc ggcgcgccaa
      901 tggtttcaaa aggagaagaa ttatttacag gggtagtacc aattttagta gaacttgacg
      961 gagatgtaaa tggacacaaa ttttcagtat caggagaagg agaaggagat gcaacatacg
     1021 gaaaattaac acttaaattc atttgtacaa caggtaaact accggttcca tggcccacac
     1081 ttgtgactac tttaacatgg ggagtacaat gtttttcaag atatccagat catatgaaac
     1141 aacacgactt ttttaaatca gcaatgcctg aaggatacgt acaagaaaga acaatatttt
     1201 ttaaagatga tggtaactat aaaacgagag cagaagtaaa atttgaagga gatacattag
     1261 taaacagaat tgaattaaaa ggaatagact ttaaagaaga tggaaatatc ctaggacata
     1321 aattagaata caattacatc agtcataacg tatacataac ggcagataaa cagaaaaatg
     1381 gaattaaagc taactttaaa ataagacaca atatagaaga cggatcagta caattagcag
     1441 atcactatca acaaaataca cctatcggag atggcccagt gcttctacca gataatcatt
     1501 acttaagcac gcaaagcgct ttatctaaag acccaaatga aaaaagagac cacatggtat
     1561 tattagaatt cgtaacagca gcaggaatta cactaggaat ggatgaatta tataagtaag
     1621 gatcctataa gttttaataa aactttaaat agaaaaaggc ttctctcatg agaagtcttt
     1681 tttatttaaa ataaatataa aataaaatag aggctataaa tagcctctat tttatgtgag
     1741 aaatccctaa ataaaaagat gccagtgtgt gctggaattc gcccttaggc tgcgctaggg
     1801 acctctttag ctccttggaa gctgtcagta gtaggggatc ggtcttgcct tgctcgtcgg
     1861 tgatgtactt caccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct
     1921 tggcaatcac gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc
     1981 gggcggacca cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc
     2041 agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag
     2101 agtttcagca ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg
     2161 tgctcatagt ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc
     2221 gacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg
     2281 cagtacacct tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
     2341 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
     2401 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
     2461 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
     2521 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
     2581 atgaccccga agcaggaagg gcgaattctg cagatctgac cggtctctga aaatataaaa
     2641 accacagatt gatactaaaa ccttggttgt gttgcttttc ggggcttaaa tcaaggaaaa
     2701 atccttgttt taagcctttc aaaaagaaac acaaggtctt tgtactaacc tgtggttatg
     2761 tataaaattg tagattttag ggtaacaaaa aacaccgtat ttctacgatg tttttgctta
     2821 aatacttgtt tttagttaca gacaaacctg aagttaacta tttatcaatt cctgcaattc
     2881 gtttacaaaa cggcaaatgt gaaatccgtc acatactgcg tgatgaactt gaattgccaa
     2941 aggaagtata attttgttat cttctttata atatttcccc atagtaaaaa taggaatcaa
     3001 ataatcatat cctttctgca aattcagatt aaagccatcg aaggttgacc acggtatcat
     3061 agatacatta aaaatgtttt ccggagcatt tggctttcct tccattctat gattgtttcc
     3121 ataccgttgc gtatcacttt cataatctgc taaaaatgat ttaaagtcag acttacactc
     3181 agtccaaagg ctggaaaatg tttcagtatc attgtgaaat attgtatagc ttggtatcat
     3241 ctcatcatat atccccaatt caccatcttg attgattgcc gtcctaaact ctgaatggcg
     3301 gtttacaatc attgcaatat aataaagcat tgcaggatat agtttcattc ccttttcctt
     3361 tatttgtgtg atatccactt taacggtcat gctgtaggta caaggtacac ttgcaaagta
     3421 gtggtcaaaa tactcttttc tgttccaact atttttatca attttttcaa ataccatcta
     3481 agttccctct caaattcaag tttatcgctc taatgaacaa agatattata ccacattttt
     3541 gtgaattttt caacttgccc acttcgactg cactcccgac ttaataactt cttgaacact
     3601 tgccgaaaaa gaaaaactgc cgggtacgta cccggggatc gatccccggc cgagcgctct
     3661 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3721 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3781 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3841 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3901 cgaaacccga caggactata aagataccag gcgtttcccc cttgaagctc cctcgtgcgc
     3961 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4021 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4081 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4141 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4201 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4261 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4321 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4381 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4441 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4501 atgaaatgca agtttctaac taacatacat catagttact aaactatggt aactataatt
     4561 ttattaacta tattaagcac tgattagtac tataactcaa tataagcata tcccctgtat
     4621 cgtaactaga gaaccaaacg acggaaaaag cgatatagat aagtttagat agtgcaccaa
     4681 ttttttactt ttctatttta gaatctagca tttccaatgc ttactaatac tatttttgat
     4741 ttttaacttg ttttaaaaaa gcacctacta attaggattc tagctctata ttctagttat
     4801 acattctcta tattctagct ctatattcta gttatacatt ctctatattc tagctctata
     4861 ttctagttat acattctcta tattctagct ctatattcta gttatacatt ctctatattc
     4921 tagctctata ttctagttat acattctcta tattctagct ctatattcta gttatacatt
     4981 ctctatattc tagctctata ttctagttat acattctcta aaaaacatat atatatatac
     5041 tacatatata tgtacaatta ttttaccaaa agaaattttt aaaacggctt aatttcaata
     5101 ctttgatgcc attttttttt tgcaattctc gtacaattat tttaccaaaa gaaattttta
     5161 ttaattagtt tttttgcata aaaataggac tagaaaatca attctaatcc tatcaaaaat
     5221 ctacttgttt aatctatatg tatagtacat aaatcctcta tcatttattt tagtttccca
     5281 tacatctaaa attttagtat ctaataaaga ttctatcata tttgaaattc tttgctgtgt
     5341 tactcctaaa tcttttgcta attcctcttg attaattctt aatatatttc cttttatttt
     5401 atcatctttg accaataaat cttgcttata tctcatatga gtatataatc ttaattcttc
     5461 agttgaaatt attccccaaa ttactgccat ggtaacaaag taactaatag tatattgttt
     5521 atctatatta catctaactt cttttacatt gtatgtatct tttattccta gctttgtaac
     5581 accttttgtt tttgtaatat aatcattatg ttgaagttct tttaatgttt ctcttaatgt
     5641 cttttcactc ataacaatgt ttttaacttt cttcttacgt ttataggtta tcagctccat
     5701 tatatcgtct atgtttaatt ctctatcttt attgttaagt aacacattat agataaacaa
     5761 ttgattacca gtcattattt taaccccttt cctattctta tatttcaaat cctttgagtg
     5821 cttatgtggc atattgaaca aagtatcttc atcactatat ataaaatctg attctatttt
     5881 attccaacaa tcactattaa actcacaacc tgtacaacca aaattaaccg ttttaagctt
     5941 ctcatacata tacttgactt gatattctag ctcactattt gaaagactat tttgtgatat
     6001 atagttccat tctctagcaa cggatattac ttgacttaaa cttttattcc tcaatcttaa
     6061 agtcacaact attttttgca aatctaggtt tttatgtcca tctttactac cattttttaa
     6121 aatatctact atacaatttg gtagcttact atctaatata tattttgtat tagtattacc
     6181 tactgttttt acatctttgc aatatttttt agctaacttt tctatgtcat atctatatat
     6241 atttttgtct tggtgtatta tttttacttg tttagtagta tttttaatat tatatgtata
     6301 aggtactctt aatacttgag tagataaatt tgcttgttta tctgcaccca atttatttat
     6361 taatgtattt gtaacttctg atactaactt aatgttatta gttttattaa ttagcacata
     6421 aacatggaat ccatttccac tatcaactag gatatgataa tgtaatccta tcttagtaaa
     6481 taaattaatt atatctttat aattaaaacc ttgtcctaat tcttttttgt caaaatcaaa
     6541 tcctaatgca tatctattta ttaagttttc attggttcct gcttttccat cagttgtaca
     6601 taggttaaaa aatgtatttt cataataaat attttttgcg aaggtaaatt ctactatatc
     6661 atctatattt ttaaaaaaag ttgttttact atattcttta aattcttctc cgagttctaa
     6721 ttgtttacta ttacttttat tattttgaaa tactctaaca tattcattat caataagatt
     6781 atttccttgt cctataccat ctctaaatac tgccattaga aattttttca acttatattt
     6841 tgaatctaat tgttccatat atttattatc tccttatcta gtttttttaa gagataacac
     6901 tacattaaat atatacttat tgactaatca acttttacaa ctattgaata attttagctt
     6961 agtatgtata ataatattgt taagatgcta aaattgatag caatattatt atataactac
     7021 agatacttaa tcagttagac cgatattgga gttggtagct ccataaactc aattggtagt
     7081 tgagttgtca ttgtctagta gtattatctc attttgttgt tatcttaaaa tattatttta
     7141 aaaatactct gcttggtcgg cttagggtat tttttttgtt taattccaat attggttcaa
     7201 actcctgtgt aatacttttg acacttccat ctgtattgaa ttgacagttg atatggtatc
     7261 tcattattgg cttgttctta tgtttttcta tgtatttatt ttttactttc aaataatttg
     7321 tatttccatt tttagttatg tttaacacac tatccttatt actcaataaa attatttttt
     7381 gttttaacat atgatttata gaatctaaaa attgatattg acttataata gtactttcta
     7441 cctctctagc tttttctagt gtttcaaatt cttcaaactc tgctatattg ctatagtata
     7501 gcttatcatt aataatatta tctgacaact ttaattccat tttataaaac aattccaagt
     7561 tatgtataaa atttttaatg ttacaacata tagacttatt atttgttctt atatggtatc
     7621 tccatttact acttctagct ctcatgttat atgcacaact actcccaaca taaacatata
     7681 ttgcttgttc taactcatat ttttgtaaaa tttcaacatc atatccagtc aaatcgtact
     7741 ctctcttata tacaaatggt ttttctaatt ttttgttcaa taaatctcct cctatttctt
     7801 acttaattta gctaaaaatc tatttatatt tcacctcctt tttgacttta agcctacgaa
     7861 taccatattt atgattataa catcttctat ttttcccaaa tccttacatc tcccctaatt
     7921 tttagactta agggcgaatt ctgcatcaa
//
