LOCUS       V003827                 7961 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003827
VERSION     V003827
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7961)
  AUTHORS   Ransom EM, Williams KB, Weiss DS, Ellermeier CD.
  TITLE     Identification and characterization of a gene cluster required for
            proper rod shape, cell division, and pathogenesis in Clostridium
            difficile
  JOURNAL   J. Bacteriol. 196 (12), 2290-2300 (2014)
   PUBMED   24727226
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   Ransom EM, Ellermeier CD, Weiss DS.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2015) Microbiology, The University of Iowa, 51
            Newton Rd, Iowa City, IA 52242, USA
REFERENCE   3  (bases 1 to 7961)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7961)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2014"; volume: "196"; issue: "12"; pages:
            "2290-2300"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-AUG-2015) Microbiology, The University of Iowa, 51 Newton Rd,
            Iowa City, IA 52242, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(58..681)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     protein_bind    788..806
                     /label="tet operator"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     CDS             873..1589
                     /label="ECFP"
                     /note="enhanced CFP"
     CDS             complement(1980..2348)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(2381..2490)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(2869..3489)
                     /gene="catP"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from Clostridium
                     perfringens. Accession#: P26826"
     rep_origin      complement(3852..4440)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5233..6870)
                     /codon_start=1
                     /gene="repA"
                     /product="RepA"
                     /label="repA"
                     /note="plasmid replication protein"
                     /protein_id="AMA07817.1"
                     /translation="MEQLDSKYKLKKFLMAVFRDGIGQGNNLIDNEYVRVFQNNKSNSK
                     QLELGEEFKEYSKTTFFKNIDDIVEFTFAKNIYYENTFFNLCTTDGKAGTNENLINRYA
                     LGFDFDKKELGQGFNYKDIINLFTKIGLHYHILVDSGNGFHVYVLINKTNNIKLVSEVT
                     NTLINKLGADKQANLSTQVLRVPYTYNIKNTTKQVKIIHQDKNIYRYDIEKLAKKYCKD
                     VKTVGNTNTKYILDSKLPNCIVDILKNGSKDGHKNLDLQKIVVTLRLRNKSLSQVISVA
                     REWNYISQNSLSNSELEYQVKYMYEKLKTVNFGCTGCEFNSDCWNKIESDFIYSDEDTL
                     FNMPHKHSKDLKYKNRKGVKIMTGNQLFIYNVLLNNKDRELNIDDIMELITYKRKKKVK
                     NIVMSEKTLRETLKELQHNDYITKTKGVTKLGIKDTYNVKEVRCNIDKQYTISYFVTMA
                     VIWGIISTEELRLYTHMRYKQDLLVKDDKIKGNILRINQEELAKDLGVTQQRISNMIES
                     LLDTKILDVWETKINDRGFMYYTYRLNK"
     gene            complement(5233..6870)
                     /gene="repA"
                     /label="repA"
     CDS             complement(7145..7657)
                     /codon_start=1
                     /gene="orfB"
                     /product="OrfB"
                     /label="orfB"
                     /protein_id="AMA07818.1"
                     /translation="MRARSSKWRYHIRTNNKSICCNIKNFIHNLELFYKMELKLSDNII
                     NDKLYYSNIAEFEEFETLEKAREVESTIISQYQFLDSINHMLKQKIILLSNKDSVLNIT
                     KNGNTNYLKVKNKYIEKHKNKPIMRYHINCQFNTDGSVKSITQEFEPILELNKKNTLSR
                     PSRVFLK"
     gene            complement(7145..7657)
                     /gene="orfB"
                     /label="orfB"
ORIGIN
        1 gctagcataa aaataagaag cctgcatttg caggcttctt atttttatgg taccttaaga
       61 cccactttca catttaagtt gtttttctaa tccgcatatg atcaattcaa ggccgaataa
      121 gaaggctggc tctgcacctt ggtgatcaaa taattcgata gcttgtcgta ataatggcgg
      181 catactatca gtagtaggtg tttccctttc ttctttagcg acttgatgct cttgatcttc
      241 caatacgcaa cctaaagtaa aatgccccac agcgctgagt gcatataatg cattctctag
      301 tgaaaaacct tgttggcata aaaaggctaa ttgattttcg agagtttcat actgtttttc
      361 tgtaggccgt gtacctaaat gtacttttgc tccatcgcga tgacttagta aagcacatct
      421 aaaactttta gcgttattac gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa
      481 gtgagtatgg tgcctatcta acatctcaat ggctaaggcg tcgagcaaag cccgcttatt
      541 ttttacatgc caatacaatg taggctgctc tacacctagc ttctgggcga gtttacgggt
      601 tgttaaacct tcgattccga cctcattaag cagctctaat gcgctgttaa tcactttact
      661 tttatctaat ctagacatca ttaattcctc ctttttgttg acattatatc attgatagag
      721 ttatttgtca aactagtttt ttatttcgat gccctggact tcatgaaaaa ctaaaaaaaa
      781 tattgacact ctatcattga tagagtataa ttaaaataag cttgatcgta gcgttaacag
      841 atctgagctc ctgcagtaaa ggagaaaatt ttatggtttc aaaaggagaa gaattattta
      901 caggggtagt accaatttta gtagaacttg acggagatgt aaatggacac aaattttcag
      961 tatcaggaga aggagaagga gatgcaacat acggaaaatt aacacttaaa ttcatttgta
     1021 caacaggtaa actaccggtt ccatggccca cacttgtgac tactttaaca tggggagtac
     1081 aatgtttttc aagatatcca gatcatatga aacaacacga cttttttaaa tcagcaatgc
     1141 ctgaaggata cgtacaagaa agaacaatat tttttaaaga tgatggtaac tataaaacga
     1201 gagcagaagt aaaatttgaa ggagatacat tagtaaacag aattgaatta aaaggaatag
     1261 actttaaaga agatggaaat atcctaggac ataaattaga atacaattac atcagtcata
     1321 acgtatacat aacggcagat aaacagaaaa atggaattaa agctaacttt aaaataagac
     1381 acaatataga agacggatca gtacaattag cagatcacta tcaacaaaat acacctatcg
     1441 gagatggccc agtgcttcta ccagataatc attacttaag cacgcaaagc gctttatcta
     1501 aagacccaaa tgaaaaaaga gaccacatgg tattattaga attcgtaaca gcagcaggaa
     1561 ttacactagg aatggatgaa ttatataaga tgaacgcatg cgtcgactta attaaacagc
     1621 tgaggcgcgc cggatcctat aagttttaat aaaactttaa atagaaaaag gcttctctca
     1681 tgagaagtct tttttattta aaataaatat aaaataaaat agaggctata aatagcctct
     1741 attttatgtg agaaatccct aaataaaaag atgccagtgt gtgctggaat tcgcccttag
     1801 gctgcgctag ggacctcttt agctccttgg aagctgtcag tagtagggga tcggtcttgc
     1861 cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg atggagcgca
     1921 tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat
     1981 ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc ctgcttctct
     2041 tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg
     2101 tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt gatgcgggcc
     2161 agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg gccgacggcc
     2221 agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat cgctcttcgt
     2281 tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg cttggtttca
     2341 tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca
     2401 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc
     2461 gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag
     2521 tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa
     2581 aaaatcgcta taatgacccc gaagcaggaa gggcgaattc tgcagatctg accggtctct
     2641 gaaaatataa aaaccacaga ttgatactaa aaccttggtt gtgttgcttt tcggggctta
     2701 aatcaaggaa aaatccttgt tttaagcctt tcaaaaagaa acacaaggtc tttgtactaa
     2761 cctgtggtta tgtataaaat tgtagatttt agggtaacaa aaaacaccgt atttctacga
     2821 tgtttttgct taaatacttg tttttagtta cagacaaacc tgaagttaac tatttatcaa
     2881 ttcctgcaat tcgtttacaa aacggcaaat gtgaaatccg tcacatactg cgtgatgaac
     2941 ttgaattgcc aaaggaagta taattttgtt atcttcttta taatatttcc ccatagtaaa
     3001 aataggaatc aaataatcat atcctttctg caaattcaga ttaaagccat cgaaggttga
     3061 ccacggtatc atagatacat taaaaatgtt ttccggagca tttggctttc cttccattct
     3121 atgattgttt ccataccgtt gcgtatcact ttcataatct gctaaaaatg atttaaagtc
     3181 agacttacac tcagtccaaa ggctggaaaa tgtttcagta tcattgtgaa atattgtata
     3241 gcttggtatc atctcatcat atatccccaa ttcaccatct tgattgattg ccgtcctaaa
     3301 ctctgaatgg cggtttacaa tcattgcaat ataataaagc attgcaggat atagtttcat
     3361 tcccttttcc tttatttgtg tgatatccac tttaacggtc atgctgtagg tacaaggtac
     3421 acttgcaaag tagtggtcaa aatactcttt tctgttccaa ctatttttat caattttttc
     3481 aaataccatc taagttccct ctcaaattca agtttatcgc tctaatgaac aaagatatta
     3541 taccacattt ttgtgaattt ttcaacttgc ccacttcgac tgcactcccg acttaataac
     3601 ttcttgaaca cttgccgaaa aagaaaaact gccgggtacg tacccgggga tcgatccccg
     3661 gccgagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     3721 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     3781 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     3841 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     3901 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc cccttgaagc
     3961 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4021 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4081 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4141 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4201 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4261 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     4321 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4381 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4441 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     4501 gggattttgg tcatgaaatg caagtttcta actaacatac atcatagtta ctaaactatg
     4561 gtaactataa ttttattaac tatattaagc actgattagt actataactc aatataagca
     4621 tatcccctgt atcgtaacta gagaaccaaa cgacggaaaa agcgatatag ataagtttag
     4681 atagtgcacc aattttttac ttttctattt tagaatctag catttccaat gcttactaat
     4741 actatttttg atttttaact tgttttaaaa aagcacctac taattaggat tctagctcta
     4801 tattctagtt atacattctc tatattctag ctctatattc tagttataca ttctctatat
     4861 tctagctcta tattctagtt atacattctc tatattctag ctctatattc tagttataca
     4921 ttctctatat tctagctcta tattctagtt atacattctc tatattctag ctctatattc
     4981 tagttataca ttctctatat tctagctcta tattctagtt atacattctc taaaaaacat
     5041 atatatatat actacatata tatgtacaat tattttacca aaagaaattt ttaaaacggc
     5101 ttaatttcaa tactttgatg ccattttttt tttgcaattc tcgtacaatt attttaccaa
     5161 aagaaatttt tattaattag tttttttgca taaaaatagg actagaaaat caattctaat
     5221 cctatcaaaa atctacttgt ttaatctata tgtatagtac ataaatcctc tatcatttat
     5281 tttagtttcc catacatcta aaattttagt atctaataaa gattctatca tatttgaaat
     5341 tctttgctgt gttactccta aatcttttgc taattcctct tgattaattc ttaatatatt
     5401 tccttttatt ttatcatctt tgaccaataa atcttgctta tatctcatat gagtatataa
     5461 tcttaattct tcagttgaaa ttattcccca aattactgcc atggtaacaa agtaactaat
     5521 agtatattgt ttatctatat tacatctaac ttcttttaca ttgtatgtat cttttattcc
     5581 tagctttgta acaccttttg tttttgtaat ataatcatta tgttgaagtt cttttaatgt
     5641 ttctcttaat gtcttttcac tcataacaat gtttttaact ttcttcttac gtttataggt
     5701 tatcagctcc attatatcgt ctatgtttaa ttctctatct ttattgttaa gtaacacatt
     5761 atagataaac aattgattac cagtcattat tttaacccct ttcctattct tatatttcaa
     5821 atcctttgag tgcttatgtg gcatattgaa caaagtatct tcatcactat atataaaatc
     5881 tgattctatt ttattccaac aatcactatt aaactcacaa cctgtacaac caaaattaac
     5941 cgttttaagc ttctcataca tatacttgac ttgatattct agctcactat ttgaaagact
     6001 attttgtgat atatagttcc attctctagc aacggatatt acttgactta aacttttatt
     6061 cctcaatctt aaagtcacaa ctattttttg caaatctagg tttttatgtc catctttact
     6121 accatttttt aaaatatcta ctatacaatt tggtagctta ctatctaata tatattttgt
     6181 attagtatta cctactgttt ttacatcttt gcaatatttt ttagctaact tttctatgtc
     6241 atatctatat atatttttgt cttggtgtat tatttttact tgtttagtag tatttttaat
     6301 attatatgta taaggtactc ttaatacttg agtagataaa tttgcttgtt tatctgcacc
     6361 caatttattt attaatgtat ttgtaacttc tgatactaac ttaatgttat tagttttatt
     6421 aattagcaca taaacatgga atccatttcc actatcaact aggatatgat aatgtaatcc
     6481 tatcttagta aataaattaa ttatatcttt ataattaaaa ccttgtccta attctttttt
     6541 gtcaaaatca aatcctaatg catatctatt tattaagttt tcattggttc ctgcttttcc
     6601 atcagttgta cataggttaa aaaatgtatt ttcataataa atattttttg cgaaggtaaa
     6661 ttctactata tcatctatat ttttaaaaaa agttgtttta ctatattctt taaattcttc
     6721 tccgagttct aattgtttac tattactttt attattttga aatactctaa catattcatt
     6781 atcaataaga ttatttcctt gtcctatacc atctctaaat actgccatta gaaatttttt
     6841 caacttatat tttgaatcta attgttccat atatttatta tctccttatc tagttttttt
     6901 aagagataac actacattaa atatatactt attgactaat caacttttac aactattgaa
     6961 taattttagc ttagtatgta taataatatt gttaagatgc taaaattgat agcaatatta
     7021 ttatataact acagatactt aatcagttag accgatattg gagttggtag ctccataaac
     7081 tcaattggta gttgagttgt cattgtctag tagtattatc tcattttgtt gttatcttaa
     7141 aatattattt taaaaatact ctgcttggtc ggcttagggt attttttttg tttaattcca
     7201 atattggttc aaactcctgt gtaatacttt tgacacttcc atctgtattg aattgacagt
     7261 tgatatggta tctcattatt ggcttgttct tatgtttttc tatgtattta ttttttactt
     7321 tcaaataatt tgtatttcca tttttagtta tgtttaacac actatcctta ttactcaata
     7381 aaattatttt ttgttttaac atatgattta tagaatctaa aaattgatat tgacttataa
     7441 tagtactttc tacctctcta gctttttcta gtgtttcaaa ttcttcaaac tctgctatat
     7501 tgctatagta tagcttatca ttaataatat tatctgacaa ctttaattcc attttataaa
     7561 acaattccaa gttatgtata aaatttttaa tgttacaaca tatagactta ttatttgttc
     7621 ttatatggta tctccattta ctacttctag ctctcatgtt atatgcacaa ctactcccaa
     7681 cataaacata tattgcttgt tctaactcat atttttgtaa aatttcaaca tcatatccag
     7741 tcaaatcgta ctctctctta tatacaaatg gtttttctaa ttttttgttc aataaatctc
     7801 ctcctatttc ttacttaatt tagctaaaaa tctatttata tttcacctcc tttttgactt
     7861 taagcctacg aataccatat ttatgattat aacatcttct atttttccca aatccttaca
     7921 tctcccctaa tttttagact taagggcgaa ttctgcatca a
//
