LOCUS       40924_36313        4635 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Baculovirus expression vector pRADM, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Yao L, Sun J.
  TITLE     Construction of a baculovirus-silkworm multigene expression system 
            and its application on producing virus-like particles
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Yao L, Sun J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2011) Hen Nan Provincial Key Laboratory of Funiu 
            Mountain Insect Biology, Nan Yang Normal University, 1638 Wolong 
            Road, Nan Yang, He Nan 473061, China
REFERENCE   3  (bases 1 to 4635)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4635)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2011) Hen Nan Provincial Key Laboratory of Funiu Mountain 
            Insect Biology, Nan Yang Normal University, 1638 Wolong Road, Nan 
            Yang, He Nan 473061, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4635
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        70..174
                     /label=AmpR promoter
     CDS             175..1032
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     oriT            1229..1519
                     /label=oriT
                     /note="origin of transfer for the bacterial F plasmid
                     (Frost et al., 1994)"
     rep_origin      1537..1925
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     mobile_element  2054..2219
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     polyA_signal    complement(2248..2382)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(2635..2726)
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     promoter        2771..2880
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     polyA_signal    3016..3064
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        3581..3609
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             3798..4328
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  4398..4622
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
ORIGIN
        1 ggcgcgccgg ccggccatag ggataacagg gtaattcgaa tttcaggtgg cacttttcgg
       61 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      121 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      181 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      241 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      301 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      361 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      421 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
      481 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
      541 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
      601 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
      661 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
      721 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
      781 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
      841 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
      901 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
      961 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1021 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1081 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1141 atcccttaac gtgagttttc gttccactga gcgtcagacc cccgtagaaa ttcgaaacgc
     1201 gctagtataa tttaacccac tccacaaaaa ggctcaacag gttggtggtt ctcaccacca
     1261 aaagcaccac accccacgca aaaacaagtt tttgctgatt tttctttata aatagagtgt
     1321 tatgaaaaat tagtttctct tactctcttt atgatattta aaaaagcggt gtcggcgcgg
     1381 ctacaacaac gcgccgacac cgttttgtag gggtggtact gactattttt ataaaaaaca
     1441 ttattttata ttaggggtgc tgctagcggc gcggtgtgtt tttttatagg ataccgctag
     1501 gggcgctgct agcggtgcgt cccttctagt gtagaggatc tgttgatcag cagttcaacc
     1561 tgttgatagt acgtactaag ctctcatgtt tcacgtacta agctctcatg tttaacgtac
     1621 taagctctca tgtttaacga actaaaccct catggctaac gtactaagct ctcatggcta
     1681 acgtactaag ctctcatgtt tcacgtacta agctctcatg tttgaacaat aaaattaata
     1741 taaatcagca acttaaatag cctctaaggt tttaagtttt ataagaaaaa aaagaatata
     1801 taaggctttt aaagctttta aggtttaacg gttgtggaca acaagccagg gatgtaacgc
     1861 actgagaagc ccttagagcc tctcaaagca attttcagtg acacaggaac acttaacggc
     1921 tgacagaatt gggcccacgg agctcattac cctgttatcc ctatggccgg ccggcgcgcc
     1981 gaagatgacg gtttgtcaca tggagttggc aggatgtttg attaaaaaca taacaggaag
     2041 aaaaatgccc cgctgtgggc ggacaaaata gttgggaact gggaggggtg gaaatggagt
     2101 ttttaaggat tatttaggga agagtgacaa aatagatggg aactgggtgt agcgtcgtaa
     2161 gctaatacga aaattaaaaa tgacaaaata gtttggaact agatttcact tatctggttc
     2221 ggatctccta ggctcaagca gtgatcagat ccagacatga taagatacat tgatgagttt
     2281 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     2341 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     2401 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     2461 tacaaatgtg gtatggctga ttatgatcct ctagtacttc tcgacaagct tgtcgagact
     2521 gcaggctcta gattcgaaag cggccgcgga gctcgtcgac gtaggccttt gaattccgcg
     2581 cgcttcggac cgggatccgc gcccgatggt gggacggtat gaataatccg gaatatttat
     2641 aggttttttt attacaaaac tgttacgaaa acagtaaaat acttatttat ttgcgagatg
     2701 gttatcattt taattatctc catgatctat taatattccg gagtaggtcg cgaatcgata
     2761 ctagtatacg gacctttaat tcaacccaac acaatatatt atagttaaat aagaattatt
     2821 atcaaatcat ttgtatatta attaaaatac tatactgtaa attacatttt atttacaatc
     2881 actcgacgaa gacttgatca cccgggatct cgagccatgg tgctagcagc tgatgcatag
     2941 catgcggtac cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     3001 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
     3061 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     3121 cattgggacc aatacgcccg cgtttcttcc ttttccccac cccaaccccc aagttcgggt
     3181 gaaggcccag ggctcgcagc caacgtcggg gcggcaagcc ctgccatagc cagctttgtt
     3241 taaacaaagc tgtagccaac cactagaact atagctagag tcctgggcga acaaacgatg
     3301 ctcgccttcc agaaaaccga ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa
     3361 gcgccgcgac ggccgaggtc taccgatctc ctgaagcaag ggcagatccg tgcacagcac
     3421 cttgccgtag aagaacagca aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt
     3481 gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca aggttgccgg
     3541 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag ttgacataag cctgttcggt
     3601 tcgtaaactg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     3661 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgt
     3721 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     3781 gttatggagc agcaacgatg ttacgcagca gcaacgatgt tacgcagcag ggcagtcgcc
     3841 ctaaaacaaa gttaggtggc tcaagtatgg gcatcattcg cacatgtagg ctcggccctg
     3901 accaagtcaa atccatgcgg gctgctcttg atcttttcgg tcgtgagttc ggagacgtag
     3961 ccacctactc ccaacatcag ccggactccg attacctcgg gaacttgctc cgtagtaaga
     4021 cattcatcgc gcttgctgcc ttcgaccaag aagcggttgt tggcgctctc gcggcttacg
     4081 ttctgcccaa gtttgagcag ccgcgtagtg agatctatat ctatgatctc gcagtctccg
     4141 gcgagcaccg gaggcagggc attgccaccg cgctcatcaa tctcctcaag catgaggcca
     4201 acgcgcttgg tgcttatgtg atctacgtgc aagcagatta cggtgacgat cccgcagtgg
     4261 ctctctatac aaagttgggc atacgggaag aagtgatgca ctttgatatc gacccaagta
     4321 ccgccaccta acaattcgtt caagccgaga tcggcttccc ggccgcggag ttgttcggta
     4381 aattgtcaca acgccgcgaa tatagtcttt accatgccct tggccacgcc cctctttaat
     4441 acgacgggca atttgcactt cagaaaatga agagtttgct ttagccataa caaaagtcca
     4501 gtatgctttt tcacagcata actggactga tttcagttta caactattct gtctagttta
     4561 agactttatt gtcatagttt agatctattt tgttcagttt aagactttat tgtccgccca
     4621 cacccgctta cgcag
//
