LOCUS       62056_18730        7877 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /label=AmpR promoter
     CDS             132..989
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2509..3321
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEVHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             7033..7689
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattggagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagctc gcgaggcctc gagatctatc gattcacaaa aaataggcac acgaaaaaca
     2221 agttaaggga tgcagtttat gcatgccatg gtacccggga gctcgaattc tagaagcttc
     2281 gtattgtcgc cctacatata tacgttaaag ctaacagctg gcaaggggat acccccattc
     2341 cccgtcccag cgccccttga gcgtcataga ctcagattgt cagcttcggt cagttgacat
     2401 ttttcttatc ggcgctctac catccgtgac ggattgaagg cgctgggcgg gaaaaagctc
     2461 gccggcacga ctctccgcca ttccatctca ctcacaggag gaccccatat gagccatatt
     2521 caacgggaaa cgtcttgctc taggccgcga ttaaattcca acatggatgc tgatttatat
     2581 gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgattgtat
     2641 gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat
     2701 gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc
     2761 aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat ccccgggaaa
     2821 acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg
     2881 gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat
     2941 cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt
     3001 gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga agtgcataaa
     3061 cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt
     3121 atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac
     3181 cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag
     3241 aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat
     3301 ttgatgctcg atgagttttt ctaggatccg attcgacctg caggcatgca agctgtacgc
     3361 ctcagctacc attcttgcag ccgcgttcag ggtcttgacg tgttctttgt atctatcagc
     3421 aaactgagaa cccaattctg gatcatcctt gtaacgccaa ctctccgacc attccgaagg
     3481 aacgccttgt agtcctcctg agccgttagg gtatgtcttt agtccaatca aatgggacat
     3541 ttgcagacag gaacgcacaa gtgtctcacg tcgcttctct atttcaatat ctaaaaattc
     3601 ttcactctta tcgttgcttg acatattaaa actgtctaac tgatccagtt cacttatatg
     3661 gaaagtatca ctaaagtcat ggcttctcag aaattcaata cttcccattg atggggaccg
     3721 gttaaccaaa tcgataaaaa tttttctgtc atgtttcaga taagtttttg gcgcgttgat
     3781 tttataataa agccgccatt cttgtgccaa caacgccacg aaaagagcca cagcagcagc
     3841 ggccacatcc cattgaaaat ctttcgccag accaaccgcc aggagaacca agccgaaata
     3901 gttagcccag cgaactaaaa aactatctat aaacgtatta agaaacgttc gggtcatatt
     3961 tccgtgaatc atatcaggac gtaaatttgc acatgacgca tttccggggg cttttatccc
     4021 tcggacttct tttcaggccc cttctcggcc tttctgtgac cctgtttttt caggccgcga
     4081 actgcgcctg gtcgggcctg acccccacac gctggccttc gtactcacgc agcctgccca
     4141 gcaggtcggc cagcgcggca ttgaccacgg cggcgaggtt gcggggcggc gttccgcctg
     4201 cggtctggtc gtgcttcacg tcgcgcagca cgcgcaccag caccgcgccc acgtcgtcgg
     4261 acacgtcccg gccagcgtcg gcggcgcggg tgatgttcca aatgagtttc cgccagaagc
     4321 ccagcgagtc ggccccgtcc ccgaaagctg ccgctagcgc ccgcgcctgc ctgtccacga
     4381 tctcggcgcg gtaggccggg cgggtttccc ggtgggcgtc ggcaagctcc cacacgacgt
     4441 tttcagcgag ggagggcgcg ggccggacag tcatgttatc ggagggggag ggaagagcgg
     4501 attttatcgc ccaggtgctc acaacttcgt atgtcacggc aaccggggcg cttttttcgc
     4561 gcagttcccc gcctgtcaca tcctcacagg gttcttgact ctccggcacg ctcttaggga
     4621 gagggtggag catgttgtag acggtgcgcc cggccttcgc gtcggcgttc aggtcgcgcc
     4681 agttccggcc ccaatcgtca tgcatcaggc ggacatatcc cgccttccct tccagcacgc
     4741 gcctcggctt gagcgtcacg gcccacagcg tcccggttgc cacagactcg ccgcgcaggt
     4801 cgcccatgtg ggcatcacag gccaccagcc cgacccggcg cagatattga aggttctcgt
     4861 agaaggcgga cttcttcaga cccacatgca ccatgagcag ctcggcgggc aggtggaaca
     4921 cagcgcgggt gacgtgttcc gcatagccgc aggcgcgggc cacgtccagc gccaccgaga
     4981 acagggcgcg gaagatgcgc cgggccgact cgcggcaggg ggcgtcatct aggcaggcgg
     5041 tgagggtctg caccagttcg gcgcgtgtgg cgatggtcaa cggcgcgggc gcgggctggg
     5101 actgctgggc gatggtgggc acgggcggcg gcgagatgga ccgggccgag ctggaaattt
     5161 cccgtgtgag cgcaggttta ggcgcatggt taccttcctg cagtgaaatc tgcgaaatca
     5221 ggcccgcttc gaggagtcgg accatgaatg gattgtgtct catgaaaaaa gcctcccctc
     5281 ttgtcagagg ggagggtccc ggtctaccat gctctcagcg acgagatagc tggggtttgt
     5341 gaccctgcac tctgacaaag ccctcaccga aaggtggggg tttagtcatt tggcctagta
     5401 aagcagaaca gaggcgattt gtcacgcaaa ggcccgccgc ccgaggggaa acaggcggcg
     5461 ggccaatggc tcggcgttgt tagctggggg tcgcaccagg gaagcacaga agcccttccc
     5521 tgagcagggt ttcaccgtgt ggtgtccctt cgtccttgaa ggcgaactgg taggccaggc
     5581 cgtaatgctc ctgcaccatc ttttgctcat ccaccgcgac aaagaaacgg cgggtgttcg
     5641 ccgggctgct gagggtctgc cactcgccgg acgcgcccac cgcataggcg tctccggctc
     5701 cacattcctc ggcctgctga tactgatact tcgtccacag catcaccgcc ttatcgggca
     5761 cctgcgcgaa gctcaccagc gccccgaacc gcccggcgct cgtggtgaag gtggacgact
     5821 gcaccatgtc ggacagttcc agcgtggcag gcgcgggcgt gttcgtcggc gcgtacaccg
     5881 tccccgcctc gcacacgacc agcggggcgg cccccgtgcc gaactcggcg gcgctgccgt
     5941 ttgccagcgt gagcgccgaa gacgccaccg cgcaggtacc catgggtgcc gaccaccagc
     6001 aggtccacgc cgtgcgccgc ctcggtgagc acctcgattg gacggcccct cagggtctga
     6061 accctcccct gcacgcccgc ctccccgatg agtgtctgcg ccgtctgctg cgcctgccgg
     6121 gcattctgct ccagcatctc ccggacgtag tcgggattca gtcccgccca ctgaacgaag
     6181 ccgctgaccg gcggctcctc gatcacagtc acgacctcca gctcggcgcc tgccgccttc
     6241 ccgagcgtta tggcctgctt caaggcgcgc tggctgggct cacttccgtc aaaggccacc
     6301 acgaatcgca tccgtcctcc tccctgacca gtcaggttca gcaagcaggg taagcctctc
     6361 ccagcactcc ggggcttcac acggccttgg gtcttttctg catacccatg agcctcagta
     6421 cccgacagtt tccggcaggg cttgaaaaag ttcggctgac acgcgagaac tggacgcgtc
     6481 tcccgatgtc ctggccaagt tgacccgctg cttcaccgcg cacttcccgg agttccgcaa
     6541 gaactaggtc gagctgctct ccctcatggt cctcgccctc cttaggggca aggacgtccg
     6601 gcatgctaaa ctcgccgcgc gcttccccgg aagcgcgcac accgcctccg tcatccggcg
     6661 ggtggaacgc ttcttcgacc gtcatcctct tcggccagct gatgtcgccc gggtcgttct
     6721 gacgctcctt cccgccgcgc agccacgcga atttatcctt gaccggacca actggaagta
     6781 tgggcagacg gacgtgaacg tcttgttgct ggccgtcatt tggcgggacg tcgccatccc
     6841 cctgctctac gagttgctgc cccatggggg cagcagcgac accgagattc ggcacaccct
     6901 gatggacgat gccctgtgcc tgctgtccgc cgctgacatc cgggtgctgt attagccgac
     6961 cgcgaattcc cagcttgagt aggacaaatc cgccgagctt cgacgagatt ttcaggagct
     7021 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatcg
     7081 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     7141 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     7201 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     7261 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     7321 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     7381 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     7441 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     7501 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     7561 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
     7621 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     7681 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct
     7741 gaataagtga taataagcgg atgaatggca gaaattcgtc gaggcggcac ctcgctaacg
     7801 gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcatccc
     7861 gcaagaggcc cggcagt
//
