LOCUS       62056_18725        8661 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /label=AmpR promoter
     CDS             132..989
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1163..1751
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2193..2909)
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(2939..2967)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             3293..4105
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             7817..8473
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQSHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagct cgcgaggcct cgagaaaaat tacttgtaca gctcgtccat gccgagagtg
     2221 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
     2281 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
     2341 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
     2401 tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg
     2461 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
     2521 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     2581 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
     2641 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
     2701 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
     2761 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac
     2821 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
     2881 gtgaacagct cctcgccctt gctcaccatg aatactgttt cctgtgtgaa attccacaca
     2941 ttatacgagc cgatgattaa ttgtcaaatc tatcgattca caaaaaatag gcacacgaaa
     3001 aacaagttaa gggatgcagt ttatgcatgc catggtaccc gggagctcga attctagaag
     3061 cttcgtattg tcgccctaca tatatacgtt aaagctaaca gctggcaagg ggataccccc
     3121 attccccgtc ccagcgcccc ttgagcgtca tagactcaga ttgtcagctt cggtcagttg
     3181 acatttttct tatcggcgct ctaccatccg tgacggattg aaggcgctgg gcgggaaaaa
     3241 gctcgccggc acgactctcc gccattccat ctcactcaca ggaggacccc atatgagcca
     3301 tattcaacgg gaaacgtctt gctctaggcc gcgattaaat tccaacatgg atgctgattt
     3361 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt
     3421 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     3481 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     3541 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     3601 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     3661 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     3721 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     3781 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     3841 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     3901 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     3961 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     4021 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     4081 tcatttgatg ctcgatgagt ttttctagga tccgattcga cctgcaggca tgcaagctgt
     4141 acgcctcagc taccattctt gcagccgcgt tcagggtctt gacgtgttct ttgtatctat
     4201 cagcaaactg agaacccaat tctggatcat ccttgtaacg ccaactctcc gaccattccg
     4261 aaggaacgcc ttgtagtcct cctgagccgt tagggtatgt ctttagtcca atcaaatggg
     4321 acatttgcag acaggaacgc acaagtgtct cacgtcgctt ctctatttca atatctaaaa
     4381 attcttcact cttatcgttg cttgacatat taaaactgtc taactgatcc agttcactta
     4441 tatggaaagt atcactaaag tcatggcttc tcagaaattc aatacttccc attgatgggg
     4501 accggttaac caaatcgata aaaatttttc tgtcatgttt cagataagtt tttggcgcgt
     4561 tgattttata ataaagccgc cattcttgtg ccaacaacgc cacgaaaaga gccacagcag
     4621 cagcggccac atcccattga aaatctttcg ccagaccaac cgccaggaga accaagccga
     4681 aatagttagc ccagcgaact aaaaaactat ctataaacgt attaagaaac gttcgggtca
     4741 tatttccgtg aatcatatca ggacgtaaat ttgcacatga cgcatttccg ggggctttta
     4801 tccctcggac ttcttttcag gccccttctc ggcctttctg tgaccctgtt ttttcaggcc
     4861 gcgaactgcg cctggtcggg cctgaccccc acacgctggc cttcgtactc acgcagcctg
     4921 cccagcaggt cggccagcgc ggcattgacc acggcggcga ggttgcgggg cggcgttccg
     4981 cctgcggtct ggtcgtgctt cacgtcgcgc agcacgcgca ccagcaccgc gcccacgtcg
     5041 tcggacacgt cccggccagc gtcggcggcg cgggtgatgt tccaaatgag tttccgccag
     5101 aagcccagcg agtcggcccc gtccccgaaa gctgccgcta gcgcccgcgc ctgcctgtcc
     5161 acgatctcgg cgcggtaggc cgggcgggtt tcccggtggg cgtcggcaag ctcccacacg
     5221 acgttttcag cgagggaggg cgcgggccgg acagtcatgt tatcggaggg ggagggaaga
     5281 gcggatttta tcgcccaggt gctcacaact tcgtatgtca cggcaaccgg ggcgcttttt
     5341 tcgcgcagtt ccccgcctgt cacatcctca cagggttctt gactctccgg cacgctctta
     5401 gggagagggt ggagcatgtt gtagacggtg cgcccggcct tcgcgtcggc gttcaggtcg
     5461 cgccagttcc ggccccaatc gtcatgcatc aggcggacat atcccgcctt cccttccagc
     5521 acgcgcctcg gcttgagcgt cacggcccac agcgtcccgg ttgccacaga ctcgccgcgc
     5581 aggtcgccca tgtgggcatc acaggccacc agcccgaccc ggcgcagata ttgaaggttc
     5641 tcgtagaagg cggacttctt cagacccaca tgcaccatga gcagctcggc gggcaggtgg
     5701 aacacagcgc gggtgacgtg ttccgcatag ccgcaggcgc gggccacgtc cagcgccacc
     5761 gagaacaggg cgcggaagat gcgccgggcc gactcgcggc agggggcgtc atctaggcag
     5821 gcggtgaggg tctgcaccag ttcggcgcgt gtggcgatgg tcaacggcgc gggcgcgggc
     5881 tgggactgct gggcgatggt gggcacgggc ggcggcgaga tggaccgggc cgagctggaa
     5941 atttcccgtg tgagcgcagg tttaggcgca tggttacctt cctgcagtga aatctgcgaa
     6001 atcaggcccg cttcgaggag tcggaccatg aatggattgt gtctcatgaa aaaagcctcc
     6061 cctcttgtca gaggggaggg tcccggtcta ccatgctctc agcgacgaga tagctggggt
     6121 ttgtgaccct gcactctgac aaagccctca ccgaaaggtg ggggtttagt catttggcct
     6181 agtaaagcag aacagaggcg atttgtcacg caaaggcccg ccgcccgagg ggaaacaggc
     6241 ggcgggccaa tggctcggcg ttgttagctg ggggtcgcac cagggaagca cagaagccct
     6301 tccctgagca gggtttcacc gtgtggtgtc ccttcgtcct tgaaggcgaa ctggtaggcc
     6361 aggccgtaat gctcctgcac catcttttgc tcatccaccg cgacaaagaa acggcgggtg
     6421 ttcgccgggc tgctgagggt ctgccactcg ccggacgcgc ccaccgcata ggcgtctccg
     6481 gctccacatt cctcggcctg ctgatactga tacttcgtcc acagcatcac cgccttatcg
     6541 ggcacctgcg cgaagctcac cagcgccccg aaccgcccgg cgctcgtggt gaaggtggac
     6601 gactgcacca tgtcggacag ttccagcgtg gcaggcgcgg gcgtgttcgt cggcgcgtac
     6661 accgtccccg cctcgcacac gaccagcggg gcggcccccg tgccgaactc ggcggcgctg
     6721 ccgtttgcca gcgtgagcgc cgaagacgcc accgcgcagg tacccatggg tgccgaccac
     6781 cagcaggtcc acgccgtgcg ccgcctcggt gagcacctcg attggacggc ccctcagggt
     6841 ctgaaccctc ccctgcacgc ccgcctcccc gatgagtgtc tgcgccgtct gctgcgcctg
     6901 ccgggcattc tgctccagca tctcccggac gtagtcggga ttcagtcccg cccactgaac
     6961 gaagccgctg accggcggct cctcgatcac agtcacgacc tccagctcgg cgcctgccgc
     7021 cttcccgagc gttatggcct gcttcaaggc gcgctggctg ggctcacttc cgtcaaaggc
     7081 caccacgaat cgcatccgtc ctcctccctg accagtcagg ttcagcaagc agggtaagcc
     7141 tctcccagca ctccggggct tcacacggcc ttgggtcttt tctgcatacc catgagcctc
     7201 agtacccgac agtttccggc agggcttgaa aaagttcggc tgacacgcga gaactggacg
     7261 cgtctcccga tgtcctggcc aagttgaccc gctgcttcac cgcgcacttc ccggagttcc
     7321 gcaagaacta ggtcgagctg ctctccctca tggtcctcgc cctccttagg ggcaaggacg
     7381 tccggcatgc taaactcgcc gcgcgcttcc ccggaagcgc gcacaccgcc tccgtcatcc
     7441 ggcgggtgga acgcttcttc gaccgtcatc ctcttcggcc agctgatgtc gcccgggtcg
     7501 ttctgacgct ccttcccgcc gcgcagccac gcgaatttat ccttgaccgg accaactgga
     7561 agtatgggca gacggacgtg aacgtcttgt tgctggccgt catttggcgg gacgtcgcca
     7621 tccccctgct ctacgagttg ctgccccatg ggggcagcag cgacaccgag attcggcaca
     7681 ccctgatgga cgatgccctg tgcctgctgt ccgccgctga catccgggtg ctgtattagc
     7741 cgaccgcgaa ttcccagctt gagtaggaca aatccgccga gcttcgacga gattttcagg
     7801 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     7861 atcgcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     7921 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     7981 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     8041 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     8101 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     8161 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     8221 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     8281 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     8341 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     8401 ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     8461 gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggtgctac
     8521 gcctgaataa gtgataataa gcggatgaat ggcagaaatt cgtcgaggcg gcacctcgct
     8581 aacggattca ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgca
     8641 tcccgcaaga ggcccggcag t
//
