LOCUS       V003835                 6447 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003835
VERSION     V003835
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Helle L, Kull M, Mayer S, Marincola G, Zelder ME, Goerke C, Wolz C,
            Bertram R.
  TITLE     Vectors for improved Tet repressor-dependent gradual gene induction
            or silencing in Staphylococcus aureus
  JOURNAL   Microbiology (Reading, Engl.) 157 (PT 12), 3314-3323 (2011)
   PUBMED   21921101
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Bertram R.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2011) Dept of Microbial Genetics, University of
            Tuebingen, Waldhaeuser Str. 70/8, Tuebingen 72076, Germany
REFERENCE   3  (bases 1 to 6447)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6447)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Microbiology (Reading, Engl.) 157 (PT 12), 3314-3323 (2011)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-AUG-2011) Dept of Microbial Genetics, University of Tuebingen,
            Waldhaeuser Str. 70/8, Tuebingen 72076, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    244..265
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        280..310
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    318..334
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     342..358
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(904..1551)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     rep_origin      2009..2944
     CDS             3042..3056
                     /label="enterokinase site"
                     /note="enterokinase recognition and cleavage site"
     CDS             complement(3371..3994)
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     regulatory      complement(4010..4065)
                     /label="pR* w/o tetO"
                     /note="pR* w/o tetO"
                     /regulatory_class="promoter"
     protein_bind    4101..4119
                     /label="tet operator"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    4136..4153
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     primer_bind     complement(4189..4205)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4679..4783
                     /label="AmpR promoter"
     CDS             4784..5641
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5815..6403
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
       61 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      121 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      181 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcggacagtg agcgcaacgc
      241 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      301 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
      361 tgattacgcc aagcttctgt aggtttttag gcataaaact atatgattta cccctaaatc
      421 tttaaaatgc cccttaaaat tcaaaataaa ggcatttaaa atttaaatat ttcttgtgat
      481 aaagtttgtt aaaaaggagt ggttttatga ctgttatgtg gttatcgatt ataggtatgt
      541 ggttttgtat tggaatggca ttttttgcta tcaaggttat taaaaataaa aattagacca
      601 cgcatttatg ccgagaaaat ttattgtgcg ttgagaagaa cccttaacta aacttgcaga
      661 cgaatgtcgg catagcgtga gctattaagc cgaccattcg acaagttttg ggattgttaa
      721 gggttccgag gctcaacgtc aataaagcaa ttggaataaa gaagcgaaaa aggagaagtc
      781 ggttcagaaa aagaaggata tggatctgga gctgtaatat aaaaaccttc ttcaactaac
      841 ggggcaggtt agtgacatta gaaaaccgac tgtaaaaagt acagtcggca ttatctcata
      901 ttataaaagc cagtcattag gcctatctga caattcctga atagagttca taaacaatcc
      961 tgcatgataa ccatcacaaa cagaatgatg tacctgtaaa gatagcggta aatatattga
     1021 attaccttta ttaatgaatt ttcctgctgt aataatgggt agaaggtaat tactattatt
     1081 attgatattt aagttaaacc cagtaaatga agtccatgga ataatagaaa gagaaaaagc
     1141 attttcaggt ataggtgttt tgggaaacaa tttccccgaa ccattatatt tctctacatc
     1201 agaaaggtat aaatcataaa actctttgaa gtcattcttt acaggagtcc aaataccaga
     1261 gaatgtttta gatacaccat caaaaattgt ataaagtggc tctaacttat cccaataacc
     1321 taactctccg tcgctattgt aaccagttct aaaagctgta tttgagttta tcacccttgt
     1381 cactaagaaa ataaatgcag ggtaaaattt atatccttct tgttttatgt ttcggtataa
     1441 aacactaata tcaatttctg tggttatact aaaagtcgtt tgttggttca aataatgatt
     1501 aaatatctct tttctcttcc aattgtctaa atcaatttta ttaaagttca tttgatatgc
     1561 ctcctaaatt tttatctaaa gtgaatttag gaggcttact tgtctgcttt cttcattaga
     1621 atcaatcctt ttttaaaagt caatattact gtaacataaa tatatatttt aaaaatatcc
     1681 cactttatcc aattttcgtt tgttgaacta atgggtgctt tagttgaaga ataaaagacc
     1741 acattaaaaa atgtggtctt ttgtgttttt ttaaaggatt tgagcgtagc gaaaaatcct
     1801 tttctttctt atcttgataa taagggtaac tattgccggc gaggctagtt acccttaagt
     1861 tattggtatg actggtttta agcgcaaaaa aagttgcttt ttcgtaccta ttaatgtatc
     1921 gttttaaatg aatagtaaaa aacatacata gaaaggggaa aaagcaactt tttttattgt
     1981 catagtttgt gaaaactaag ttgtttttat gtgttataac atggaaaagt atactgagaa
     2041 aaaacaaaga aatcaagtat ttcagaaatt tattaaacgt catattggag agaatcaaat
     2101 ggatttagtt gaagattgca atacatttct gtcttttgta gctgataaaa ctttagaaaa
     2161 acagaaatta tataaagcta attcttgtaa aaatcgattt tgtcctgtct gtgcttggag
     2221 aaaagctaga aaagatgcat tgggtttatc tttgatgatg caatatatta agcagcaaga
     2281 gaaaaaggag tttatctttt taactttgac tacacctaat gtaatgagtg atgaattaga
     2341 aaatgaaata aaacgttata ataattcttt tagaaaactt ataaagagaa aaaaagtagg
     2401 tagtgttata aagggatatg ttcgtaagtt agagattaca tataataaaa aaagagatga
     2461 ttataatcct cattttcatg tgttaattgc agtaaataaa tcgtatttca cagataaaag
     2521 atattatatt agccaacaag aatggttaga tttatggcgt gatgtaacgg gcatttcaga
     2581 aataacacaa gttcaagttc aaaaaataag acaaaataat aataaagaat tatatgaaat
     2641 ggctaagtat tctggtaaag atagtgatta tttaataaat caaaaagtct ttgatgcatt
     2701 ttataaatca cttaaaggta aacaggtatt agtttattca ggattattta aagaggctaa
     2761 aaagaaatta aaaaatgggg atttagatta cttaaaagaa attgatccaa ccgaatatat
     2821 ctatcaaatt ttttatattt ggaaacaaaa agagtattta gctagtgaac tttatgactt
     2881 aacagaacaa gaaaaaagag aaattaatca caaaatgata gacgaaatcg aggaagaaca
     2941 ataacaaaat ataagtgcta acagctgacc tcccgataac accatgtagt tattgggagg
     3001 tcagctgttg aattatgcac gagtatttta aaagttattg tgatgacgac gataaacgat
     3061 tatcaaaagt ataatgttaa aatgctttat tatactaacg ttatataaac attatacttt
     3121 cgttatacaa attttaaccc tgttaggaac tataaaaaat catgaaaatt ttaatttgca
     3181 tgtaactggg cagtgtctta aaaaatcgac actgaatttg ctcaaatttt tgtttgtaga
     3241 attagaatat atttatttgg ctcatatttg ctttttaaaa gcttgcatgc ctgcaggtcg
     3301 acggtatcga taactcgaca tcttggttac cgtgaagtta ccatcacgga aaaaggttat
     3361 gctgctttta agacccactt tcacatttaa gttgtttttc taatccgcat atgatcaatt
     3421 caaggccgaa taagaaggct ggctctgcac cttggtgatc aaataattcg atagcttgtc
     3481 gtaataatgg cggcatacta tcagtagtag gtgtttccct ttcttcttta gcgacttgat
     3541 gctcttgatc ttccaatacg caacctaaag taaaatgccc cacagcgctg agtgcatata
     3601 atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc taattgattt tcgagagttt
     3661 catactgttt ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg cgatgactta
     3721 gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa atcttgccag ctttcccctt
     3781 ctaaagggca aaagtgagta tggtgcctat ctaacatctc aatggctaag gcgtcgagca
     3841 aagcccgctt attttttaca tgccaataca atgtaggctg ctctacacct agcttctggg
     3901 cgagtttacg ggttgttaaa ccttcgattc cgacctcatt aagcagctct aatgcgctgt
     3961 taatcacttt acttttatct aatctagaca tcattaattc ctcctttttg ttgacattat
     4021 atcattgata gagttatttg tcaaactagt tttttatttg gatcccctcg agttcatgaa
     4081 aaactaaaaa aaatattgac actctatcat tgatagagta taattaaaat aagctctcta
     4141 tcattgatag agtatgatgg taccgttaac agatctgagc tcgaattcac tggccgtcgt
     4201 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     4261 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     4321 gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg
     4381 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4441 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     4501 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     4561 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     4621 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     4681 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4741 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4801 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4861 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4921 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4981 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     5041 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     5101 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     5161 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     5221 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     5281 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5341 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5401 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5461 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5521 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5581 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5641 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5701 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5761 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5821 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5881 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5941 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     6001 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     6061 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     6121 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     6181 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     6241 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6301 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6361 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6421 ctttttacgg ttcctggcct tttgctg
//
