LOCUS       V016334                 2999 bp    DNA     circular SYN 06-JUN-2019
DEFINITION  Exported.
ACCESSION   V016334
VERSION     V016334
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Moore CL, Papa LJ III, Shoulders MD.
  TITLE     A Processive Protein Chimera Introduces Mutations across Defined DNA
            Regions In Vivo
  JOURNAL   J. Am. Chem. Soc. 140 (37), 11560-11564 (2018)
   PUBMED   29991261
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Moore CL, Papa LJ III, Shoulders MD.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-APR-2019) Chemistry, Massachusetts Institute of
            Technology, 77 Massachusetts Avenue, 16-573A, Cambridge, MA 02139,
            USA
REFERENCE   3  (bases 1 to 2999)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Am.
            Chem. Soc."; date: "2018"; volume: "140"; issue: "37"; pages:
            "11560-11564"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-APR-2019) Chemistry, Massachusetts Institute of Technology, 77
            Massachusetts Avenue, 16-573A, Cambridge, MA 02139, USA"
FEATURES             Location/Qualifiers
     source          1..2999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             23..325
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             368..739
                     /gene="rpsL"
                     /label="Small ribosomal subunit protein uS12"
                     /note="Small ribosomal subunit protein uS12 from Salmonella
                     paratyphi A (strain ATCC 9150 / SARB42). Accession#:
                     Q5PIW1"
     rep_origin      793..1181
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1249..1911)
                     /label="amilCP"
                     /note="amilCP is a fluorescent protein published in 2008,
                     derived from Acropora millepora. It is reported to be a
                     somewhat slowly-maturing tetramer."
     regulatory      1918..1929
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
                     /regulatory_class="ribosome_binding_site"
     promoter        complement(1965..1995)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     promoter        2073..2120
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             2139..2939
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase from Tn5"
ORIGIN
        1 cacataacca ggaggtcaga ttatgcagtt taaggtttac acctataaaa gagagagccg
       61 ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt
      121 gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac tttacccggt
      181 ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt
      241 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
      301 cattaacctg atgttctggg gaatataacc cagaagctta gcaaaagcta aaaccaggag
      361 ctatttaatg gcaacagtta accagctggt acgcaaacca cgtgctcgca aagttgcgaa
      421 aagcaacgtg cctgcgctgg aagcatgccc gcaaaaacgt ggcgtatgta ctcgtgtata
      481 tactaccact cctaaaaaac cgaactccgc gctgcgtaaa gtatgccgtg ttcgtctgac
      541 taacggtttc gaagtgactt cctacatcgg tggtgaaggt cacaacctgc aggagcactc
      601 cgtgatcctg atccgtggcg gtcgtgttaa agacctcccg ggtgttcgtt accacaccgt
      661 acgtggtgcg cttgactgct ccggcgttaa agaccgtaag caggctcgtt ccaagtatgg
      721 cgtgaagcgt cctaaggctt aatggttcgc ccgcctaatg agcgggcttt tttttgaatt
      781 cttttttaat tcgatctgaa gatcagcagt tcaacctgtt gatagtacgt actaagctct
      841 catgtttcac gtactaagct ctcatgttta acgtactaag ctctcatgtt taacgaacta
      901 aaccctcatg gctaacgtac taagctctca tggctaacgt actaagctct catgtttcac
      961 gtactaagct ctcatgtttg aacaataaaa ttaatataaa tcagcaactt aaatagcctc
     1021 taaggtttta agttttataa gaaaaaaaag aatatataag gcttttaaag cttttaaggt
     1081 ttaacggttg tggacaacaa gccagggatg taacgcactg agaagccctt agagcctctc
     1141 aaagcaattt tgagtgacac aggaacactt aacggctgac atgggatccg aattaaaaaa
     1201 gaattcaaaa aaaagcccgc tcattaggcg ggcgaaccaa ccggtttagg cgaccacagg
     1261 tttgcgtgca atggaaattt cacactgctc aaccgaagtg taatccttgt tgtgattggt
     1321 tacatccagt ttgcggtcaa catagtgata ccctggcatc ttcacaggct tctttgcctt
     1381 gtaagtagtt ttaaattcac acaaatagtg accgcctcct tctaacttca gagccataaa
     1441 gttgtttcct agcagcattc catctcgtgc aaagagacgc tcagtgttgg gttcccagcc
     1501 ctgtgtcttc ttctgcatga caggtccatt gggaggaaag ttcaaaccag agaacttgac
     1561 atggtagatg aaacagttgc cttggatgct ggaatcattg ctgacagtac acactgcacc
     1621 atcttcaaag ttcatgatcc tctcccatgt atagccctcc gggaatgact gctttacata
     1681 gtcagggatg tcttcagggt acttggtgaa tggtatgctt ccgtactgac actgtggtga
     1741 taaaatatcc caagcaaatg gcagaggtcc gcccttggtg acagtgagct ttaccgtctg
     1801 ctccccctcg tagggcttac cttttccatc gccttcgacc tcaaagtagt gtccattgac
     1861 cgtgcctgac atataaacct tgtaggtcat ttgtttagcg atcacactca tctagtattt
     1921 ctcctcttta attactagat ccacacatta taggtacaaa aagacattat acgagccgga
     1981 agcataaagt gtaaaggtac ccatcagtgc caacatagta agccagtata cactccgcta
     2041 gcgcggccgc ctcgagtttc gacctgcagc ctgttgacaa ttaatcatcg gcatagtata
     2101 tcggcatagt ataatacgac aaggtgagga actaaaccat gggatcggcc attgaacaag
     2161 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     2221 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     2281 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     2341 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     2401 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     2461 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     2521 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     2581 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     2641 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gatgatctcg
     2701 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     2761 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     2821 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     2881 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     2941 gaggggatca attctctaga gctcgctgat cagcctcgac tgtaccgtta gccctccca
//
