LOCUS       62056_18695        7127 bp DNA     circular SYN 06-APR-2019
DEFINITION  Transformation vector pQUSV, complete sequence.
ACCESSION   MK424965
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Luo J, Shen P, Chen J.
  TITLE     A modular toolset of phiC31-based fluorescent protein tagging 
            vectors for Drosophila
  JOURNAL   Fly (Austin) (2019) In press
  PUBMED    30885036
REFERENCE   2  (bases 1 to 7127)
  AUTHORS   Luo J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2019) Model Animal Research Center, Nanjing 
            University, 12 Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China
REFERENCE   3  (bases 1 to 7127)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JAN-2019) Model Animal Research Center, Nanjing University, 12 
            Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China"
FEATURES             Location/Qualifiers
     source          1..7127
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    667..2546
                     /label=vermilion
                     /note="vermilion"
     protein_bind    2553..2586
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2587..3017
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     misc_feature    3033..3126
                     /label=5xQUAS
                     /note="5xQUAS"
     promoter        3175..3413
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    3418..3472
                     /label=multiple cloning site
                     /note="multiple cloning site"
     intron          3550..3615
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3745..3765
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4037..4171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    4182..4612
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    4651..4720
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(4939..4957)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4978..4994)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5002..5018)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5026..5056)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5071..5092)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5380..5968)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6142..6999)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7000..7104)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggccccattt gtgtgcgccg cactttcacc tcaagtgatt gataattccc agcctatctg
      721 gcagtgccca tcgcccagat caccgactgt gcaatcagtc ggaactggag ctctctcgct
      781 ctgttatcgg ttcgctgggg tctcatctcc ggtccgctgg cggagatcag ttcgccagca
      841 tccgccgctc gaggagtcac gatctgatct gagctgtgca ccatgagctg tccctatgca
      901 ggaaacgggt gagcaccagc acgtgctgtc caggaatgcc aatcgatctt cagttctgcg
      961 attcaattca aacccataca gaaacgatca cgatgattcg gcggtgccat taaccacgga
     1021 agtgggcaaa atctatggag agtatctgat gctggacaaa ctgctggatg cccagtgtat
     1081 gctgtccgag gaggacaagc gacccgtgca cgatgagcat ctgttcatca tcacgcacca
     1141 gggtgagtag gtttacaact ttgatgacaa cactcaatgg catttaagta ccttcgccac
     1201 agcctacgag ctttggttca agcagatcat ctttgagttc gactccatac gagacatgtt
     1261 ggatgcagag gtcatcgatg aaaccaagac gctggagatt gtcaagcgac tgaaccgagt
     1321 ggttctgatt ctaaaagtga gtgctttctg aatctcttac caaaatccgt ttataacttc
     1381 ctttgtacag ctcctggtgg accaagtgcc cattctggag accatgaccc cgctagactt
     1441 catggacttc cgcaagtacc tggcacccgc atctggtttt cagtcgctgc agttccgttt
     1501 gatcgagaac aagctgggag ttctgacaga gcagcgggtg agatacaacc agaagtactc
     1561 ggatgtcttt agcgacgagg aggcgcggaa ctcgattcgc aactcggaga aagatccctc
     1621 gctactggag ctagtgcagc gatggctgga gaggacgccc ggactggagg agagtggctt
     1681 caacttctgg gccaagtttc aggagagcgt cgatcgattc ctggaggcgc aggtacagag
     1741 cgccatggag gagcccgtgg agaaggcgaa aaactaccgc ctcatggaca ttgagaagcg
     1801 acgcgaggtg tatcgctcca tctttgatcc ggcagtgcac gatgcactgg tgcgtcgtgg
     1861 ggatcgccgg tttagccatc gtgcccttca gggagccatc atgatcacct tctataggga
     1921 tgaacccagg ttcagccaac cacaccagtt gctcaccctg ctcatggaca tcgactcgtt
     1981 aataaccaag tggagatgta agtattgcat tctttgatac tcttttataa atatatctta
     2041 tgtttaagac tggttttcct aaccaaatac tttctattcc cgccgcagac aatcacgtga
     2101 tcatggtgca acgcatgatt ggatcccaac agttgggcac tggtggctcg tctggatatc
     2161 aatatctgcg ctccactctc aggtgatcat cgcagatgtg attatatcgg ggatcaatga
     2221 actcaaactg ttctcccttt gttttttttt ggtttcagtg atcggtacaa ggtgtttctg
     2281 gatctgttca atctgtccac ttttctgatt ccccgcgagg cgattccacc gctggacgag
     2341 accattcgca agaaactgat caacaaaagt gtctgacaat cggcagggta tccaattcgt
     2401 caatgtttgg ctatgcgttg tttgttctgc ctactgtttt gtcgttttgg tgtaataaaa
     2461 ttacttgttt agtctttgtt atcacatttg atgtgttcct tttctttatg tctgacatat
     2521 aatacatata acataacaaa ataaatgcta gtataacttc gtataatgta tgctatacga
     2581 agttattggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     2641 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     2701 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     2761 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     2821 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaagaat
     2881 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     2941 ataccttttc ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg
     3001 gtgtgccaac caacaacacg cgtactggat ccgggtaatc gcttatcctc ggataaacaa
     3061 ttatcctcac gggtaatcgc ttatccgctc gggtaatcgc ttatcctcgg gtaatcgctt
     3121 atccttaagc ttgatatcga attgctgcag cccgagcgga gactctagcg agcgccggag
     3181 tataaataga ggcgcttcgt ctacggagcg acaattcaat tcaaacaagc aaagtgaaca
     3241 cgtcgctaag cgaaagctaa gcaaataaac aagcgcagct gaacaagcta aacaatctgc
     3301 agtaaagtgc aagttaaagt gaatcaatta aaagtaacca gcaaccaagt aaatcaactg
     3361 caactactga aatctgccaa gaagtaatta ttgaatacaa gaagagaact ctgaataggg
     3421 aattgggaat tcgttaacag atctgcggcc gcggctcgag ggtacctcta gaggatcttt
     3481 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     3541 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     3601 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     3661 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     3721 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     3781 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     3841 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     3901 aaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
     3961 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     4021 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4081 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4141 actcatcaat gtatcttatc atgtctggat cctcaaggcc ttggccacgt aataagtgtg
     4201 cgttgaattt attcgcaaaa acattgcata ttttcggcaa agtaaaattt tgttgcatac
     4261 cttatcaaaa aataagtgct gcatactttt tagagaaacc aaataatttt ttattgcata
     4321 cccgttttta ataaaataca ttgcataccc tcttttaata aaaaatattg catactttga
     4381 cgaaacaaat tttcgttgca tacccaataa aagattatta tattgcatac ccgtttttaa
     4441 taaaatacat tgcataccct cttttaataa agaatattgc atacgttgac gaaacaaatt
     4501 ttcgttgcat acccaataaa agattattat attgcatacc ttttcttgcc ataccattta
     4561 gccgatcaat tgtgctcggc aacagtatat ttgtggtgtg ccaaccaaca acactagaga
     4621 tccactagtg tcgacgatgt aggtcacggt ctcgaagccg cggtgcgggt gccagggcgt
     4681 gcccttgggc tccccgggcg cgtactccac ctcacccatc tggtccatca tgatgaacgg
     4741 gtcgaggtgg cggtagttga tcccggcgaa cgcgcggcgc accgggaagc cctcgccctc
     4801 gaaaccgctg ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt cggcgggtgc
     4861 ggatacgcgg ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt cgacactagt
     4921 tctagccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc
     4981 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     5041 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     5101 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     5161 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     5221 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     5281 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     5341 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     5401 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     5461 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     5521 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     5581 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     5641 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     5701 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     5761 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     5821 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5881 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5941 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6001 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6061 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6121 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6181 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6241 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6301 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6361 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6421 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6481 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6541 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     6601 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     6661 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     6721 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     6781 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6841 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6901 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6961 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7021 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7081 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
