LOCUS       40924_36263        4825 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQTEV2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ergin A, Thiel A, Duchmann R, Sieper J, Bussow K, Adam T.
  TITLE     Homologous High-Throughput Expression and Purification of Highly 
            Conserved E. coli Proteins
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Ergin A, Adam T, Bussow K.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-FEB-2007) Gastroenterology, Charite Berlin (CBF), 
            Hindenburgdamm 30, Berlin 12200, Germany
REFERENCE   3  (bases 1 to 4825)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4825)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-FEB-2007) Gastroenterology, Charite Berlin (CBF), Hindenburgdamm
            30, Berlin 12200, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      101..107
                     /regulatory_class="ribosome_binding_site"
     misc_feature    115
                     /note="translation start site for inserts cloned into the 
                     multiple cloning site"
     CDS             121..141
                     /label=7xHis
                     /note="6xHis affinity tag"
     protein_bind    155..175
                     /label=attB1
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     CDS             178..198
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    195..230
                     /note="multiple cloning site; includes BamHI, SphI, NotI 
                     restriction sites"
     protein_bind    complement(236..260)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      282..376
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             420..1076
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1144..1230
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(1289..1310)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1326..2405)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(2406..2483)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    2675..2815
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3001..3589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3763..4620)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4621..4725)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaaa
      121 catcaccatc accatcacca tggtgctgca ggtacaagtt tgtacaaaaa agcaggcgag
      181 aatctttatt ttcagggatc cagtcttcgc atgcatgaag acgcggccgc ctaggaccca
      241 gctttcttgt acaaagtggt aagcttaatt agctgagctt ggactcctgt tgatagatcc
      301 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
      361 ttttattggt gagaatccaa gctagcttgg cgagattttc aggagctaag gaagctaaaa
      421 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
      481 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
      541 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
      601 acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg
      661 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
      721 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
      781 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
      841 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
      901 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
      961 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat ggcttccatg
     1021 tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat
     1081 ttttttaagg cagttattgg tgcccttaaa cgcctggggt aatgactctc tagcttgagg
     1141 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     1201 tcggtgaacg ctctcctgag taggacaaat ccgccctcta gattacgtgc agtcgatgat
     1261 aagctgtcaa acatgagaat tgtgcctaat gagtgagcta acttacatta attgcgttgc
     1321 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     1381 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
     1441 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
     1501 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
     1561 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
     1621 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
     1681 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
     1741 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
     1801 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
     1861 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg
     1921 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
     1981 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
     2041 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
     2101 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
     2161 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
     2221 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
     2281 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
     2341 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
     2401 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
     2461 gttttgcacc attcgatggt gtcggaattt cgggcagcgt tgggtcctgg ccacgggtgc
     2521 gcatgatcta gagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
     2581 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
     2641 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
     2701 atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     2761 ccacatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     2821 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     3481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4801 tatcacgagg ccctttcgtc ttcac
//
