LOCUS       40924_36253        3499 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQStrep2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Scheich C, Sievert V, Bussow K.
  TITLE     An automated method for high-throughput protein purification applied
            to a comparison of His-tag and GST-tag affinity chromatography
  JOURNAL   BMC Biotechnol. 3, 12 (2003)
  PUBMED    12885298
REFERENCE   2  (bases 1 to 3499)
  AUTHORS   Bussow K, Tischer J.
  TITLE     Cloning vector pQStrep2
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3499)
  AUTHORS   Bussow K, Tischer J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2001) Dept. Prof. H. Lehrach, Max Planck Institute
            of Molecular Genetics, Ihnestr. 73, Berlin 14195, Germany
REFERENCE   4  (bases 1 to 3499)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3499)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol. 3, 12 (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (19-MAR-2001) Dept. Prof. H. Lehrach, Max Planck Institute of 
            Molecular Genetics, Ihnestr. 73, Berlin 14195, Germany"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      101..107
                     /regulatory_class="ribosome_binding_site"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    145..197
                     /note="multiple cloning site for BamHI, SalI, BglII and
                     NotI"
     CDS             199..222
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     terminator      245..339
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             383..1039
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1107..1193
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1349..1489
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1675..2263)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2437..3294)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3295..3399)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc gcatgcgagc tcggtacccc gggtcgacct
      181 gcaagatctg cggccgcttg gagccacccg cagttcgaaa aataagctta attagctgag
      241 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
      301 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caagctagct tggcgagatt
      361 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      421 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      481 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      541 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      601 tcgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      661 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      721 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      781 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      841 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      901 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      961 tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1021 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
     1081 ggtaatgact ctctagcttg aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1141 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
     1201 tctagagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     1261 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     1321 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg
     1381 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     1441 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc
     1501 ttcctcgctc actgactcgc tgcgctcggt ctgtcggctg cggcgagcgg tatcagctca
     1561 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1621 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1681 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1741 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1801 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1861 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1921 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1981 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2041 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2101 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2161 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2221 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2281 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2341 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2401 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2461 tatctcagcg atctgtctat ttcgttcatc catagctgcc tgactccccg tcgtgtagat
     2521 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2581 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2641 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2701 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2761 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2821 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2881 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2941 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3001 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3061 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3121 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3181 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3241 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3301 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3361 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3421 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     3481 gaggcccttt cgtcttcac
//
