LOCUS       V016493                 4889 bp    DNA     circular SYN 27-FEB-2021
DEFINITION  Exported.
ACCESSION   V016493
VERSION     V016493
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Park S.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4889)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (03-DEC-2020) Food Science "
FEATURES             Location/Qualifiers
     source          1..4889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..996
                     /gene="repF"
                     /label="repF"
     CDS             1..996
                     /codon_start=1
                     /transl_table=11
                     /gene="repF"
                     /product="RepF"
                     /label="repF"
                     /note="replication protein"
                     /protein_id="QRY06366.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     rep_origin      1179..1567
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     regulatory      1580..1824
                     /label="sarA P1 promoter"
                     /note="sarA P1 promoter"
                     /regulatory_class="promoter"
     CDS             1847..2329
                     /gene="dfrA"
                     /label="Dihydrofolate reductase type 1"
                     /note="Dihydrofolate reductase type 1 from Tn4003 from
                     Staphylococcus aureus. Accession#: P13955"
     regulatory      2340..2639
                     /label="blaZ transcription terminator"
                     /note="blaZ transcription terminator"
                     /regulatory_class="terminator"
     regulatory      2658..2795
                     /label="agr P3 promoter"
                     /note="agr P3 promoter"
                     /regulatory_class="promoter"
     CDS             2815..3522
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     regulatory      3575..3632
                     /label="atl transcription terminator"
                     /note="atl transcription terminator"
                     /regulatory_class="terminator"
     CDS             complement(4467..4478)
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaagctat
      121 gcagatatac tagaaatttt aaaaatcaaa aaggctttta atgtaaaaca atgtggtaat
      181 attttagaat ttaagccaac tgatgaaggc tatttgaagt tacataagac atggttttgt
      241 aaatcaaaat tatgtccggt ttgtaattgg agacgtgcta tgaaaaatag ttatcaagct
      301 caaaaagtga ttgaaaaagt aattaaggaa aagccaaaag cacgttggtt gtttttaaca
      361 ctttcaacaa aaaatgcgat agatggagat actttagaac aaagtttgaa gcatctaact
      421 aaagcatttg ataggttgag tagatataaa aaggttaaac aaaatcttgt tggatttatg
      481 cgttcaacag aagttaccgt taataaaaat gacggtagtt ataatcagca catgcatgtt
      541 ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga attatataac tcaagaagaa
      601 tgggttaatt tatggcaaag agcattacaa gttgactatc gacctgttgc taatgttaaa
      661 gcgatcaaac cgaatagaaa aggcgataaa gacattgaat cggcaatcaa agagacctca
      721 aaatattcgg ttaaatcatc tgatttttta actgatgatg atgaaaaaaa tcaagaaatt
      781 gtaagtgatt tagaaaaagg tttgtatcga aaacgtatgt taagttatgg tggattgctt
      841 aaacaaaaac ataaaatttt aaacttagac gatgtcgaag atggtaattt gattaatgca
      901 agtgatgaag ataaaacaac agacgaagaa gaaaaagcac attcaattac cgcaatttgg
      961 aatttcgaaa agcaaaatta ttatttaaga cattagtgtt gactaatgtc ttttttgttg
     1021 attttttata aaaaagtact gtcttatttt tgtgacaaat gctgtatgta gtgtcacaaa
     1081 aataagacaa acgcaatata ttgtgtcaca aaaataagac agtacagctt tgtatgatcc
     1141 gtcgacgaaa gcctggccac gatgcgtccg gcgtagagga tctgaagatc agcagttcaa
     1201 cctgttgata gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt
     1261 actaagctct catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc
     1321 taacgtacta agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa
     1381 tataaatcag caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata
     1441 tataaggctt ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac
     1501 gcactgagaa gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg
     1561 gctgacatgg gaattcgagc tgatattttt gactaaacca aatgctaacc cagaaataca
     1621 atcactgtgt ctaatgaata atttgtttta taaacacttt tttgtttact tctcattttt
     1681 aattagttat aattaactaa ataatagagc attaaatata tttaataaaa cttatttaat
     1741 gcaaaattat gactaacata tctataataa ataaagatta gatatcaata tattatcggg
     1801 caaatgtatc gagcaagatg catcggatcg atccaggagg tataccatga cattatcaat
     1861 aattgtcgct cacgataaac aaagagtcat tgggtaccaa aatcaattac cttggcactt
     1921 accaaatgat ttaaagcata ttaaacaact gaccactggg aatacacttg taatggcacg
     1981 gaaaactttt aattctatag ggaagccatt gccaaataga cgtaacgtcg tactcactaa
     2041 ccaagcttca tttcgccatg aaggggtaga tgttataaac tctcttgatg aaattaaaga
     2101 gttatctggt catgttttta tatttggagg acaaacgtta tacgaagcaa tgattgacca
     2161 ggtagatgat atgtatatca cagtaataga tggaaagttt caaggagaca cattctttcc
     2221 accatacaca ttcgaaaact gggaagtcga atcttcagta gaaggtcaac tagatgaaaa
     2281 aaatactata ccgcatacat tcttacattt agtgcgtaga aaagggaaat aggcgcgcct
     2341 attctaatgc ataataaata ctgataacat cttatatttt gtattatatt ttgtattatc
     2401 gttgacatgt ataattttga tatcaaaaac tgattttccc tctattattt tcgagattta
     2461 ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatt ataaataata
     2521 gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt
     2581 gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag
     2641 gcgatgcggc cgctagcgta atttgtattt aatattttaa cataaaaaaa tttacagtta
     2701 agaataaaaa acgactagtt aagaaaaaat ggaaaataaa tgcttttagc atgttttaat
     2761 ataactagat cacagagatg tgatggaaaa tagttgaagg aaggagtgtc tagaatggtg
     2821 agcaagggtg aggaggataa catggcaatc atcaaggagt tcatgcgttt taaagttcat
     2881 atggaaggtt cagttaatgg tcatgaattc gaaatcgagg gcgagggcga gggtcgtcca
     2941 tatgagggca cacaaaccgc aaagttgaag gtgacaaagg gtggtccatt gccattcgca
     3001 tgggacatct tatcacctca atttatgtat ggttcaaaag catatgttaa gcatccagca
     3061 gacatcccag actatttgaa gttgtcattc ccagagggct tcaagtggga gcgtgtgatg
     3121 aacttcgagg acggcggtgt ggttacagtg acacaagact catcattgca agatggtgaa
     3181 tttatttata aagttaagtt gcgtggtaca aacttcccat cagacggccc agtaatgcag
     3241 aagaagacaa tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     3301 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     3361 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     3421 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     3481 gagggccgcc actccaccgg cggcatggac gagctgtaca agtagggatc cgaatcatga
     3541 attacaagca aaagtagcgg tgattgttaa aattgatggt aaacaatcac cgctattttt
     3601 gcttgtgtat gtataaaaaa gggatcaaag gtcatccccc atgattgata gtggggggat
     3661 gacttttgat cctatgttca tgttgcttat ttaatcgcct ttgatcactt taaaatacct
     3721 taaaacccct ggaatttctg gctttgccag acctatcatt tttgaatgat agcaaattct
     3781 ccttatgctc ttacggagtt tttagagaaa aattaaaaat tctcgatttt tgataaaaaa
     3841 cgccctgcag gaatttagaa aaacatgtgg aagttttaaa ggattttatg ctaattttta
     3901 atttgcatgt aactcgaggg gaatatttga ggggattttg aaacgagttt cttcttgttt
     3961 tcacactgtt tttttattcc tattggtgtt gttgcttact ttttgttttt ctataaagat
     4021 gatgcttttg acgagatgga agaaaaatat gattatcatg aagataataa aaaatagacg
     4081 acgcatttat gccgagaaaa tttattgatg ttgagaagaa cccttaacta aacttgcaga
     4141 cgaatgtcgg catagcgtga gctattaagc cgaccattcg acaagttttg ggattgttaa
     4201 gggttccgag gctcaacgtc aataaagcaa ttggaataaa tagtatctaa agtaacccgt
     4261 atttaagatt acattgtaca tccttcgaaa ccatagtagc tgcgattcca gctaggtttc
     4321 cttttatccc aattttagct aatttttttg ctacgccctt taagtttttg gctttaacat
     4381 cttcaaaaac tgctgtgatt atgtttccag ttaaaagttc tccatatgag tttaaaactt
     4441 cagaatagaa acagtcaccg ctatttttgt agttcccacc aactgctcta ggtgatattg
     4501 aactagtttt ctttttattc tctatgtcgt tttttagttt ttggaatcct tcaggaacat
     4561 atccatatcg agatttggcc gattttattt gataatcctt ttgacttttt agaaagtttt
     4621 atttattcat tggttttcac attaatttat ttaggtttag agggtcataa aaagaagaag
     4681 gaataggttg ttttttgaaa cgagtgtgaa cgagtttctt cttgtcttga tactatatag
     4741 aaataactcg attttatata tatagctgta actgttgata ttacagtgtt taaacgtgtt
     4801 tttgtgcgtg aaaggaaaat ttgacaataa aaaaccccag ttatattatt aaggtgtcga
     4861 atcttaaata atactggggg tctttttat
//
