LOCUS       V015962                 4906 bp    DNA     circular SYN 27-FEB-2021
DEFINITION  Exported.
ACCESSION   V015962
VERSION     V015962
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Park S.
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4906)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (03-DEC-2020) Food Science "
FEATURES             Location/Qualifiers
     source          1..4906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..996
                     /gene="repF"
                     /label="repF"
     CDS             1..996
                     /codon_start=1
                     /transl_table=11
                     /gene="repF"
                     /product="RepF"
                     /label="repF"
                     /note="replication protein"
                     /protein_id="QRY06363.1"
                     /translation="MQYNTTRSITENQDNKTLKDMTKSGKQRPWREKKIDNVSYADILE
                     ILKIKKAFNVKQCGNILEFKPTDEGYLKLHKTWFCKSKLCPVCNWRRAMKNSYQAQKVI
                     EKVIKEKPKARWLFLTLSTKNAIDGDTLEQSLKHLTKAFDRLSRYKKVKQNLVGFMRST
                     EVTVNKNDGSYNQHMHVLLCVENAYFRKKENYITQEEWVNLWQRALQVDYRPVANVKAI
                     KPNRKGDKDIESAIKETSKYSVKSSDFLTDDDEKNQEIVSDLEKGLYRKRMLSYGGLLK
                     QKHKILNLDDVEDGNLINASDEDKTTDEEEKAHSITAIWNFEKQNYYLRH"
     rep_origin      1179..1567
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     regulatory      1580..1824
                     /label="sarA P1 promoter"
                     /note="sarA P1 promoter"
                     /regulatory_class="promoter"
     CDS             1847..2329
                     /gene="dfrA"
                     /label="Dihydrofolate reductase type 1"
                     /note="Dihydrofolate reductase type 1 from Tn4003 from
                     Staphylococcus aureus. Accession#: P13955"
     regulatory      2340..2639
                     /label="blaZ transcription terminator"
                     /note="blaZ transcription terminator"
                     /regulatory_class="terminator"
     regulatory      2658..2795
                     /label="agr P3 promoter"
                     /note="agr P3 promoter"
                     /regulatory_class="promoter"
     CDS             2821..3534
                     /label="GFPuv"
                     /note="GFP variant optimized for excitation by UV light"
     regulatory      3592..3649
                     /label="atl transcription terminator"
                     /note="atl transcription terminator"
                     /regulatory_class="terminator"
     CDS             complement(4484..4495)
                     /label="WELQut site"
                     /note="WELQut protease recognition and cleavage site"
ORIGIN
        1 atgcaatata atactactag aagtataacc gaaaatcaag ataataaaac gttaaaagat
       61 atgacgaaaa gtgggaaaca acgcccatgg agagaaaaga aaatagataa tgtaagctat
      121 gcagatatac tagaaatttt aaaaatcaaa aaggctttta atgtaaaaca atgtggtaat
      181 attttagaat ttaagccaac tgatgaaggc tatttgaagt tacataagac atggttttgt
      241 aaatcaaaat tatgtccggt ttgtaattgg agacgtgcta tgaaaaatag ttatcaagct
      301 caaaaagtga ttgaaaaagt aattaaggaa aagccaaaag cacgttggtt gtttttaaca
      361 ctttcaacaa aaaatgcgat agatggagat actttagaac aaagtttgaa gcatctaact
      421 aaagcatttg ataggttgag tagatataaa aaggttaaac aaaatcttgt tggatttatg
      481 cgttcaacag aagttaccgt taataaaaat gacggtagtt ataatcagca catgcatgtt
      541 ttgttatgtg ttgaaaatgc atattttaga aaaaaagaga attatataac tcaagaagaa
      601 tgggttaatt tatggcaaag agcattacaa gttgactatc gacctgttgc taatgttaaa
      661 gcgatcaaac cgaatagaaa aggcgataaa gacattgaat cggcaatcaa agagacctca
      721 aaatattcgg ttaaatcatc tgatttttta actgatgatg atgaaaaaaa tcaagaaatt
      781 gtaagtgatt tagaaaaagg tttgtatcga aaacgtatgt taagttatgg tggattgctt
      841 aaacaaaaac ataaaatttt aaacttagac gatgtcgaag atggtaattt gattaatgca
      901 agtgatgaag ataaaacaac agacgaagaa gaaaaagcac attcaattac cgcaatttgg
      961 aatttcgaaa agcaaaatta ttatttaaga cattagtgtt gactaatgtc ttttttgttg
     1021 attttttata aaaaagtact gtcttatttt tgtgacaaat gctgtatgta gtgtcacaaa
     1081 aataagacaa acgcaatata ttgtgtcaca aaaataagac agtacagctt tgtatgatcc
     1141 gtcgacgaaa gcctggccac gatgcgtccg gcgtagagga tctgaagatc agcagttcaa
     1201 cctgttgata gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt
     1261 actaagctct catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc
     1321 taacgtacta agctctcatg tttcacgtac taagctctca tgtttgaaca ataaaattaa
     1381 tataaatcag caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata
     1441 tataaggctt ttaaagcttt taaggtttaa cggttgtgga caacaagcca gggatgtaac
     1501 gcactgagaa gcccttagag cctctcaaag caattttgag tgacacagga acacttaacg
     1561 gctgacatgg gaattcgagc tgatattttt gactaaacca aatgctaacc cagaaataca
     1621 atcactgtgt ctaatgaata atttgtttta taaacacttt tttgtttact tctcattttt
     1681 aattagttat aattaactaa ataatagagc attaaatata tttaataaaa cttatttaat
     1741 gcaaaattat gactaacata tctataataa ataaagatta gatatcaata tattatcggg
     1801 caaatgtatc gagcaagatg catcggatcg atccaggagg tataccatga cattatcaat
     1861 aattgtcgct cacgataaac aaagagtcat tgggtaccaa aatcaattac cttggcactt
     1921 accaaatgat ttaaagcata ttaaacaact gaccactggg aatacacttg taatggcacg
     1981 gaaaactttt aattctatag ggaagccatt gccaaataga cgtaacgtcg tactcactaa
     2041 ccaagcttca tttcgccatg aaggggtaga tgttataaac tctcttgatg aaattaaaga
     2101 gttatctggt catgttttta tatttggagg acaaacgtta tacgaagcaa tgattgacca
     2161 ggtagatgat atgtatatca cagtaataga tggaaagttt caaggagaca cattctttcc
     2221 accatacaca ttcgaaaact gggaagtcga atcttcagta gaaggtcaac tagatgaaaa
     2281 aaatactata ccgcatacat tcttacattt agtgcgtaga aaagggaaat aggcgcgcct
     2341 attctaatgc ataataaata ctgataacat cttatatttt gtattatatt ttgtattatc
     2401 gttgacatgt ataattttga tatcaaaaac tgattttccc tctattattt tcgagattta
     2461 ttttcttaat tctctttaac aaactagaaa tattgtatat acaaaaaatt ataaataata
     2521 gatgaatagt ttaattatag gtgttcatca atcgaaaaag caacgtatct tatttaaagt
     2581 gcgttgcttt tttctcattt ataaggttaa ataattctca tatatcaagc aaagtgacag
     2641 gcgatgcggc cgctagcgta atttgtattt aatattttaa cataaaaaaa tttacagtta
     2701 agaataaaaa acgactagtt aagaaaaaat ggaaaataaa tgcttttagc atgttttaat
     2761 ataactagat cacagagatg tgatggaaaa tagttgggat ccaaggaagg agtgtctaga
     2821 atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     2881 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     2941 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
     3001 gtcactactt tctcttatgg tgttcaatgc ttttcccgtt atccggatca tatgaaacgg
     3061 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc
     3121 aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt
     3181 aatcgtatcg agttaaaagg tgttgatttt aaagaagatg gaaacattct cggacacaaa
     3241 ctcgagtaca actataactc acacaatgta tacatcacgg cagacaaaca aaagaatgga
     3301 atcaaagcta acttcaaaat tcgccacaac attgaagatg gtaccgttca actagcagac
     3361 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     3421 ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt
     3481 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaataagat
     3541 ggggatccga atcatgaatt acaagcaaaa gtagcggtga ttgttaaaat tgatggtaaa
     3601 caatcaccgc tatttttgct tgtgtatgta taaaaaaggg atcaaaggtc atcccccatg
     3661 attgatagtg gggggatgac ttttgatcct atgttcatgt tgcttattta atcgcctttg
     3721 atcactttaa aataccttaa aacccctgga atttctggct ttgccagacc tatcattttt
     3781 gaatgatagc aaattctcct tatgctctta cggagttttt agagaaaaat taaaaattct
     3841 cgatttttga taaaaaacgc cctgcaggaa tttagaaaaa catgtggaag ttttaaagga
     3901 ttttatgcta atttttaatt tgcatgtaac tcgaggggaa tatttgaggg gattttgaaa
     3961 cgagtttctt cttgttttca cactgttttt ttattcctat tggtgttgtt gcttactttt
     4021 tgtttttcta taaagatgat gcttttgacg agatggaaga aaaatatgat tatcatgaag
     4081 ataataaaaa atagacgacg catttatgcc gagaaaattt attgatgttg agaagaaccc
     4141 ttaactaaac ttgcagacga atgtcggcat agcgtgagct attaagccga ccattcgaca
     4201 agttttggga ttgttaaggg ttccgaggct caacgtcaat aaagcaattg gaataaatag
     4261 tatctaaagt aacccgtatt taagattaca ttgtacatcc ttcgaaacca tagtagctgc
     4321 gattccagct aggtttcctt ttatcccaat tttagctaat ttttttgcta cgccctttaa
     4381 gtttttggct ttaacatctt caaaaactgc tgtgattatg tttccagtta aaagttctcc
     4441 atatgagttt aaaacttcag aatagaaaca gtcaccgcta tttttgtagt tcccaccaac
     4501 tgctctaggt gatattgaac tagttttctt tttattctct atgtcgtttt ttagtttttg
     4561 gaatccttca ggaacatatc catatcgaga tttggccgat tttatttgat aatccttttg
     4621 actttttaga aagttttatt tattcattgg ttttcacatt aatttattta ggtttagagg
     4681 gtcataaaaa gaagaaggaa taggttgttt tttgaaacga gtgtgaacga gtttcttctt
     4741 gtcttgatac tatatagaaa taactcgatt ttatatatat agctgtaact gttgatatta
     4801 cagtgtttaa acgtgttttt gtgcgtgaaa ggaaaatttg acaataaaaa accccagtta
     4861 tattattaag gtgtcgaatc ttaaataata ctgggggtct ttttat
//
