LOCUS       V003847                 5955 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003847
VERSION     V003847
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Swofford CA, Van Dessel N, Forbes NS.
  TITLE     Quorum-sensing Salmonella selectively trigger protein expression
            within tumors
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 112 (11), 3457-3462 (2015)
   PUBMED   25737556
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Swofford CA, Van Dessel N, Forbes NS.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2014) Chemical Engineering, University of
            Massachusetts Amherst, 686 North Pleasant Street, Amherst, MA
            01003-9303, USA
REFERENCE   3  (bases 1 to 5955)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5955)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2015"; volume: "112"; issue: "11"; pages:
            "3457-3462"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-DEC-2014) Chemical Engineering, University of Massachusetts
            Amherst, 686 North Pleasant Street, Amherst, MA 01003-9303, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..616)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             961..2064
                     /gene="asd"
                     /label="Aspartate-semialdehyde dehydrogenase"
                     /note="Aspartate-semialdehyde dehydrogenase from Salmonella
                     typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
                     Accession#: P0A1F8"
     terminator      complement(2225..2319)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(2343..2999)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3000..3102)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     regulatory      3132..3276
                     /label="luxI"
                     /note="luxI"
                     /regulatory_class="promoter"
     CDS             3312..4268
                     /gene="hly"
                     /label="Alpha-hemolysin"
                     /note="Alpha-hemolysin from Staphylococcus aureus (strain
                     NCTC 8325 / PS 47). Accession#: Q2G1X0"
     RBS             4351..4362
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             4370..5119
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             5153..5731
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
ORIGIN
        1 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
       61 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta
      241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
      481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      661 cgaaaactca cgttaaggga ttttggtcat gacgtacgtt ttcgttccat tggccctcaa
      721 acccctaatt aggatcaata aaacagcgac ggaaatgatt cccttcctaa cgcaaattcc
      781 ctgataatcg ccactggact ttctgcttgc gcggtaaggc aggataagtc gcattactga
      841 tggcttcgct atcattgatt aatttcactt gcgactttgg ctgctttttg tatggtgaag
      901 gatgcgccac aggatactgg cgcgcataca cagcacatct ctttgcagga aaaaaacgct
      961 atgaaaaatg ttggttttat cggctggcgc ggaatggtcg gctctgttct catgcaacgc
     1021 atggtagagg agcgcgattt cgacgctatt cgccctgttt tcttttctac ctcccagttt
     1081 ggacaggcgg cgcccacctt cggcgacacc tccaccggca cgctacagga cgcttttgat
     1141 ctggatgcgc taaaagcgct cgatatcatc gtgacctgcc agggcggcga ttataccaac
     1201 gaaatttatc caaagctgcg cgaaagcgga tggcagggtt actggattga tgcggcttct
     1261 acgctgcgca tgaaagatga tgccattatt attctcgacc cggtcaacca ggacgtgatt
     1321 accgacggcc tgaacaatgg cgtgaagacc tttgtgggcg gtaactgtac cgttagcctg
     1381 atgttgatgt cgctgggcgg tctctttgcc cataatctcg ttgactgggt atccgtcgcg
     1441 acctatcagg ccgcctccgg cggcggcgcg cgccatatgc gcgagctgtt aacccagatg
     1501 ggtcagttgt atggccatgt cgccgatgaa ctggcgacgc cgtcttccgc aattcttgat
     1561 attgaacgca aagttacggc attgacccgc agcggcgagc tgccggttga taactttggc
     1621 gtaccgctgg cgggaagcct gatcccctgg atcgacaaac agctcgataa cggccagagc
     1681 cgcgaagagt ggaaaggcca ggcggaaacc aacaagattc tcaatactgc ctctgtgatt
     1741 ccggttgatg gtttgtgtgt gcgcgtcggc gcgctgcgct gtcacagcca ggcgttcacc
     1801 atcaagctga aaaaagaggt atccattccg acggtggaag aactgctggc ggcacataat
     1861 ccgtgggcga aagtggtgcc gaacgatcgt gatatcacta tgcgcgaatt aaccccggcg
     1921 gcggtgaccg gcacgttgac tacgccggtt ggtcgtctgc gtaagctgaa catggggcca
     1981 gagttcttgt cggcgtttac cgtaggcgac cagttgttat ggggcgccgc cgagccgctg
     2041 cgtcgaatgc tgcgccagtt ggcgtagtgg ctattgcagc gcttatcggg cctgcgtgtg
     2101 gttctgtagg ccggataagg cgcgtcagcg ccgccatccg gcggggaaat ttgtgttaaa
     2161 ccaggggtgc atcgtcaccc tttttttgcg taatacagga gtaaacgcag atactagtgc
     2221 ttggattctc accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     2281 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     2341 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     2401 tggaagccat cacagacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     2461 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     2521 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     2581 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     2641 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     2701 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     2761 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     2821 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     2881 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     2941 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     3001 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     3061 atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccag
     3121 atatcgacgt cagtcctttg attctaataa attggatttt tgtcacacta ttgtatcgct
     3181 gggaatacaa ttacttaaca taagcacctg taggatcgta caggtttacg caagaaaatg
     3241 gtttgttata gtcgaataaa cgcaagggag gttggtgcta gctcgtttaa aaaaatagaa
     3301 ggatgatgaa aatgaaaaca cgtatagtca gctcagtaac aacaacacta ttgctaggtt
     3361 ccatattaat gaatcctgtc gctaatgccg cagattctga tattaatatt aaaaccggta
     3421 ctacagatat tggaagcaat actacagtaa aaacaggtga tttagtcact tatgataaag
     3481 aaaatggcat gcacaaaaaa gtattttata gttttatcga tgataaaaat cacaataaaa
     3541 aactgctagt tattagaacg aaaggtacca ttgctggtca atatagagtt tatagcgaag
     3601 aaggtgctaa caaaagtggt ttagcctggc cttcagcctt taaggtacag ttgcaactac
     3661 ctgataatga agtagctcaa atatctgatt actatccaag aaattcgatt gatacaaaag
     3721 agtatatgag tactttaact tatggattca acggtaatgt tactggtgat gatacaggaa
     3781 aaattggcgg ccttattggt gcaaatgttt cgattggtca tacactgaaa tatgttcaac
     3841 ctgatttcaa aacaatttta gagagcccaa ctgataaaaa agtaggctgg aaagtgatat
     3901 ttaacaatat ggtgaatcaa aattggggac catatgatag agattcttgg aacccggtat
     3961 atggcaatca acttttcatg aaaactagaa atggttctat gaaagcagca gataacttcc
     4021 ttgatcctaa caaagcaagt tctctattat cttcagggtt ttcaccagac ttcgctacag
     4081 ttattactat ggatagaaaa gcatccaaac aacaaacaaa tatagatgta atatacgaac
     4141 gagttcgtga tgattaccaa ttgcattgga cttcaacaaa ttggaaaggt accaatacta
     4201 aagataaatg gacagatcgt tcttcagaaa gatataaaat cgattgggaa aaagaagaaa
     4261 tgacaaatta acctgcaggc atgcaagctt aatgcggtag tttatcacag ttaaattgct
     4321 aacgcagtca ggcaccgtgt atcgatcgtt aaagaggaga aaggtaccca tgaaaaacat
     4381 aaatgccgac gacacataca gaataattaa taaaattaaa gcttgtagaa gcaataatga
     4441 tattaatcaa tgcttatctg atatgactaa aatggtacat tgtgaatatt atttactcgc
     4501 gatcatttat cctcattcta tggttaaatc tgatatttca attctagata attaccctaa
     4561 aaaatggagg caatattatg atgacgctaa tttaataaaa tatgatccta tagtagatta
     4621 ttctaactcc aatcattcac caattaattg gaatatattt gaaaacaatg ctgtaaataa
     4681 aaaatctcca aatgtaatta aagaagcgaa aacatcaggt cttatcactg ggtttagttt
     4741 ccctattcat acggctaaca atggcttcgg aatgcttagt tttgcacatt cagaaaaaga
     4801 caactatata gatagtttat ttttacatgc gtgtatgaac ataccattaa ttgttccttc
     4861 tctagttgat aattatcgaa aaataaatat agcaaataat aaatcaaaca acgatttaac
     4921 caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt gggatatttc
     4981 aaaaatatta ggctgcagtg agcgtactgt cactttccat ttaaccaatg cgcaaatgaa
     5041 actcaataca acaaaccgct gccaaagtat ttctaaagca attttaacag gagcaattga
     5101 ttgcccatac tttaaaaatt aagcggccgg aataaacgca agggaggttg gtatgactat
     5161 aatgataaaa aaatcggatt ttttggcaat tccatcggag gagtataaag gtattctaag
     5221 tcttcgttat caagtgttta agcaaagact tgagtgggac ttagttgtag aaaataacct
     5281 tgaatcagat gagtatgata actcaaatgc agaatatatt tatgcttgtg atgatactga
     5341 aaatgtaagt ggatgctggc gtttattacc tacaacaggt gattatatgc tgaaaagtgt
     5401 ttttcctgaa ttgcttggtc aacagagtgc tcccaaagat cctaatatag tcgaattaag
     5461 tcgttttgct gtaggtaaaa atagctcaaa gataaataac tctgctagtg aaattacaat
     5521 gaaactattt gaagctatat ataaacacgc tgttagtcaa ggtattacag aatatgtaac
     5581 agtaacatca acagcaatag agcgattttt aaagcgtatt aaagttcctt gtcatcgtat
     5641 tggagacaaa gaaattcatg tattaggtga tactaaatcg gttgtattgt ctatgcctat
     5701 taatgaacag tttaaaaaag cagtcttaaa ttaatgttgt taaatcatta atttatttta
     5761 aatactaagt atattatagg gaaaataatg aataaatgta ttccaatgat aattaatgga
     5821 atgattcaag atcctaggcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5881 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5941 gcaaaaggcc aggaa
//
