LOCUS       40924_36233        4823 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQLinkN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Scheich C, Kummel D, Soumailakakis D, Heinemann U, Bussow K.
  TITLE     Vectors for co-expression of an unrestricted number of proteins
  JOURNAL   Nucleic Acids Res. 35 (6), E43 (2007)
  PUBMED    17311810
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Kuemmel D, Scheich C, Buessow K.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2006) Vertebrate Genomics, Max Planck Institute 
            for Molecular Genetics, Ihnestr. 63-73, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 4823)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4823)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2007"; volume: "35"; issue: "6"; pages: "E43"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-DEC-2006) Vertebrate Genomics, Max Planck Institute for 
            Molecular Genetics, Ihnestr. 63-73, Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..27
                     /label=LIC cassette with PacI restriction site
                     /note="LIC cassette with PacI restriction site"
     promoter        36..80
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    88..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             123..134
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      127..133
                     /regulatory_class="ribosome_binding_site"
     misc_feature    141
                     /note="translation start site for inserts cloned into the 
                     multiple cloning site"
     protein_bind    complement(184..208)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      230..324
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     misc_feature    329..378
                     /label=LIC cassette with SwaI, PacI restriction sites
                     /note="LIC cassette with SwaI, PacI restriction sites"
     CDS             418..1074
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1142..1228
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(1287..1308)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1324..2403)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(2404..2481)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    2673..2813
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      2997
                     /label=ColE1 ori
                     /note="ColE1 ori"
     rep_origin      complement(2999..3587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3761..4618)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4619..4723)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagctta attaacaaca ccatttgtcg agaaatcata aaaaatttat ttgctttgtg
       61 agcggataac aattataata gattcaattg tgagcggata acaatttcac acagaattca
      121 ttaaagagga gaaattaact atgggatcca gtcttcgcat gcatgaagac gcggccgcct
      181 aggacccagc tttcttgtac aaagtggtaa gcttaattag ctgagcttgg actcctgttg
      241 atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc
      301 cgggcgtttt ttattggtga gaatccaagt aacaacacca tttaaatgga gtggttacaa
      361 atggagtggt taattaaggc tagcttggcg agattttcag gagctaagga agctaaaatg
      421 gagaaaaaaa tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat
      481 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt
      541 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac
      601 attcttgccc gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag
      661 ctggtgatat gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg
      721 ttttcatcgc tctggagtga ataccacgac gatttccggc agtttctaca catatattcg
      781 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat
      841 atgtttttcg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc
      901 aatatggaca acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac
      961 aaggtgctga tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc
     1021 ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt
     1081 ttttaaggca gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca
     1141 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1201 ggtgaacgct ctcctgagta ggacaaatcc gccctctaga ttacgtgcag tcgatgataa
     1261 gctgtcaaac atgagaattg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc
     1321 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     1381 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
     1441 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
     1501 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
     1561 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag
     1621 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
     1681 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
     1741 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
     1801 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
     1861 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
     1921 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
     1981 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
     2041 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
     2101 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
     2161 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
     2221 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
     2281 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     2341 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     2401 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     2461 tttgcaccat tcgatggtgt cggaatttcg ggcagcgttg ggtcctggcc acgggtgcgc
     2521 atgatctaga gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2581 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2641 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     2701 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     2761 acatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     2821 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2881 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2941 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3001 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3061 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3121 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3181 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3241 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3301 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3361 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3421 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3481 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3541 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3601 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3661 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3721 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3781 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3841 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3901 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3961 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4021 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4081 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4141 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     4201 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4261 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4321 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4381 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4441 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4501 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4561 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4621 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4681 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4741 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     4801 tcacgaggcc ctttcgtctt cac
//
