LOCUS       40924_36203       10546 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Broad-host-range expression vector pQLICE, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10546)
  AUTHORS   Harms K, Schon V, Kickstein E, Wackernagel W.
  TITLE     The RecJ DNase strongly suppresses genomic integration of short but 
            not long foreign DNA fragments by homology-facilitated illegitimate 
            recombination during transformation of Acinetobacter baylyi
  JOURNAL   Mol. Microbiol. 64 (3), 691-702 (2007)
  PUBMED    17462017
REFERENCE   2  (bases 1 to 10546)
  AUTHORS   Kickstein E, Harms K, Wackernagel W.
  TITLE     Deletions of recBCD or recD influence genetic transformation 
            differently and are lethal together with a recJ deletion in 
            Acinetobacter baylyi
  JOURNAL   Microbiology (Reading, Engl.) 153 (PT 7), 2259-2270 (2007)
  PUBMED    17600070
REFERENCE   3  (bases 1 to 10546)
  AUTHORS   Huelter N.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2006) Genetics, Department of Biology and 
            Environmental Sciences, Carl von Ossietzky University of Oldenburg, 
            Carl-von-Ossietzky-Strasse 9-11, Oldenburg D-26111, Germany
REFERENCE   4  (bases 1 to 10546)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 10546)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. 
            Microbiol."; date: "2007"; volume: "64"; issue: "3"; pages: 
            "691-702"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Microbiology (Reading, Engl.) 153 (PT 7), 2259-2270 (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-DEC-2006) Genetics, Department of Biology and Environmental 
            Sciences, Carl von Ossietzky University of Oldenburg, 
            Carl-von-Ossietzky-Strasse 9-11, Oldenburg D-26111, Germany"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             63..866
                     /codon_start=1
                     /gene="strA"
                     /product="streptomycin resistance protein A"
                     /label=strA
                     /protein_id="ABM30607.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRR
                     GELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPS
                     MGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPLHDLLARV
                     ERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIA
                     NAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            63..866
                     /gene="strA"
                     /label=strA
     CDS             866..1702
                     /codon_start=1
                     /gene="strB"
                     /product="streptomycin resistance protein B"
                     /label=strB
                     /protein_id="ABM30608.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
     gene            866..1702
                     /gene="strB"
                     /label=strB
     misc_feature    1674..2034
                     /note="fragment; similar to transposase"
     misc_feature    2035..2050
                     /label=multiple cloning site
                     /note="multiple cloning site"
     protein_bind    complement(2065..2099)
                     /label=LacI repressor protein binding site
                     /bound_moiety="LacI repressor protein"
                     /note="lac operator"
     protein_bind    2074..2090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2098..2126)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     promoter        2360..2437
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2438..3517
                     /label=lacI
                     /note="lac repressor"
     misc_feature    3897..4075
                     /note="fragment; similar to transposase"
     rep_origin      complement(4208..4602)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(4629..4913)
                     /codon_start=1
                     /gene="mobC"
                     /product="mobilization protein C"
                     /label=mobC
                     /protein_id="ABM30610.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     gene            complement(4629..4913)
                     /gene="mobC"
                     /label=mobC
     oriT            4944..5031
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             5860..6273
                     /codon_start=1
                     /gene="mobB"
                     /product="mobilization protein B"
                     /label=mobB
                     /protein_id="ABM30612.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     gene            5860..6273
                     /gene="mobB"
                     /label=mobB
     CDS             6270..7238
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             7302..7514
                     /codon_start=1
                     /gene="E"
                     /product="hypothetical protein"
                     /label=E
                     /note="unknown protein E"
                     /protein_id="ABM30614.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     gene            7302..7514
                     /gene="E"
                     /label=E
     CDS             7516..7722
                     /codon_start=1
                     /gene="F"
                     /product="repressor protein F"
                     /label=F
                     /note="regulates repAC operon"
                     /protein_id="ABM30615.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     gene            7516..7722
                     /gene="F"
                     /label=F
     misc_RNA        7685..7759
                     /product="regulatory RNA"
     regulatory      7739..7745
                     /gene="repA"
                     /regulatory_class="ribosome_binding_site"
     CDS             7752..8588
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             8578..9426
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             9737..10525
                     /codon_start=1
                     /gene="sulII"
                     /product="sulfonamid resistance protein"
                     /label=sulII
                     /protein_id="ABM30618.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADVI
                     DLVRHPAIPTPRLFRPTQKSRVCAGAGRAQADGIPVSLDSYQPATQAYALSRGVAYLND
                     IRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAALTGA
                     GIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRALTGRG
                     PGVSGPRHSLQSLPPPQVELTSSAHTSRAPCATGWRYWRR"
     gene            9737..10525
                     /gene="sulII"
                     /label=sulII
ORIGIN
        1 aactgcacat tcgggatatt tctctatatt cgcgcttcat cagaaaactg aaggaacctc
       61 cattgaatcg aactaatatt ttttttggtg aatcgcattc tgactggttg cctgtcagag
      121 gcggagaatc tggtgatttt gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg
      181 cacctgcttc ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt tggctcaaag
      241 gtcgaggtgt ggcttgcccc gaggtcatca actggcagga ggaacaggag ggtgcatgct
      301 tggtgataac ggcaattccg ggagtaccgg cggctgatct gtctggagcg gatttgctca
      361 aagcgtggcc gtcaatgggg cagcaacttg gcgctgttca cagcctatcg gttgatcaat
      421 gtccgtttga gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca
      481 atgccgtcaa tcccgacttc ttaccggacg aggacaagag tacgccgctg cacgatcttt
      541 tggctcgtgt cgaacgagag ctaccggtgc ggctcgacca agagcgcacc gatatggttg
      601 tttgccatgg tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca
      661 cgggtctgat cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca
      721 tgattgctaa cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg
      781 tcctattcaa tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc
      841 gattggaccc tctgacttgg ggttgatgtt catgccgcct gtttttcctg ctcattggca
      901 cgtttcgcaa cctgttctca ttgcggacac cttttccagc ctcgtttgga aagtttcatt
      961 gccagacggg actcctgcaa tcgtcaaggg attgaaacct atagaagaca ttgctgatga
     1021 actgcgcggg gccgactatc tggtatggcg caatgggagg ggagcagtcc ggttgctcgg
     1081 tcgtgagaac aatctgatgt tgctcgaata tgccggggag cgaatgctct ctcacatcgt
     1141 tgccgagcac ggcgactacc aggcgaccga aattgcagcg gaactaatgg cgaagctgta
     1201 tgccgcatct gaggaacccc tgccttctgc ccttctcccg atccgggatc gctttgcagc
     1261 tttgtttcag cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc
     1321 ggcgattata gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga
     1381 tctgcatcat gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg
     1441 tctggtcggt gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga
     1501 cgacctttgt ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct
     1561 ggacgtcgat ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc
     1621 ttggaacgcg gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca
     1681 ggtgcgacag acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca
     1741 atctcaccgg agaatatcgc tggccaaagc cttagcgtag gattccgccc cttcccgcaa
     1801 acgaccccaa acaggaaacg cagctgaaac gggaagctca acacccactg acgcatgggt
     1861 tgttcaggca gtacttcatc aaccagcaag gcggcacttt cggccatccg ccgcgcccca
     1921 cagctcgggc agaaaccgcg acgcttacag ctgaaagcga ccaggtgctc ggcgtggcaa
     1981 gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag aaatggtcga
     2041 cggatccccg ggaattctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacat
     2101 tatacgagcc gatgattaat tgtcaacagc tcatttcaga atatttgcca gaaccgttat
     2161 gatgtcggcg caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattatg
     2221 cagtgattta cgacctgcac agccatacca cagcttccga tggctgcctg acgccagaag
     2281 cattggtgca ccgtgcagtc gataagctcc gccggatcag cttgcaattc gcgcgcgaag
     2341 gcgaagcggc atttacgttg acaccatcga atggtgcaaa acctttcgcg gtatggcatg
     2401 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga
     2461 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag
     2521 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     2581 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     2641 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     2701 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     2761 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     2821 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     2881 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     2941 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     3001 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     3061 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     3121 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     3181 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     3241 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgtcaaccac
     3301 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     3361 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     3421 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3481 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa
     3541 gttagcgcga attgcaagcg acgcgctggg ctacgtcttg ctggcgttcg ccttcccaac
     3601 agttgcgcag cctgaatggc gaatggaaat tgtaagcgtt aatattttgt taaaattcgc
     3661 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgactgcg atgagtggca
     3721 gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg gttgctacgc
     3781 ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc
     3841 gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt taccggtctt
     3901 caaattcccg ttgcacatag cccggcaatt cctttccctg ctctgccata agcgcagcga
     3961 atgccgggta atactcgtca acgatctgat agagaagggt ttgctcgggt cggtggctct
     4021 ggtaacgacc agtatcccga tcccggctgg ccgtcctggc cgccacatga ggcatgttcc
     4081 gcgtccttgc aatactgtgt ttacatacag tctatcgctt agcggaaagt tcttttaccc
     4141 tcagccgaaa tgcctgccgt tgctagacat tgccagccag tgcccgtcac tcccgtacta
     4201 actgtcacga acccctgcaa taactgtcac gcccccctgc aataactgtc acgaacccct
     4261 gcaataactg tcacgccccc aaacctgcaa acccagcagg ggcgggggct ggcggggtgt
     4321 tggaaaaatc catccatgat tatctaagaa taatccacta ggcgcggtta tcagcgccct
     4381 tgtggggcgc tgctgccctt gcccaatatg cccggccaga ggccggatag ctggtctatt
     4441 cgctgcgcta ggctacacac cgccccaccg ctgcgcggca gggggaaagg cgggcaaagc
     4501 ccgctaaacc ccacaccaaa ccccgcagaa atacgctgga gcgcttttag ccgctttagc
     4561 ggcctttccc cctacccgaa gggtgggggc gcgtgtgcag ccccgcaggg cctgtctcgg
     4621 tcgatcattc agcccggctc atccttctgg cgtggcggca gaccgaacaa ggcgcggtcg
     4681 tggtcgcgtt caaggtacgc atccattgcc gccatgagcc gatcctccgg ccactcgctg
     4741 ctgttcacct tggccaaaat catggccccc accagcacct tgcgccttgt ttcgttcttg
     4801 cgctcttgct gctgttccct tgcccgcacc cgctgaattt cggcattgat tcgcgctcgt
     4861 tgttcttcga gcttggccag ccgatccgcc gccttgttgc tccccttaac catcttgaca
     4921 ccccattgtt aatgtgctgt ctcgtaggct atcatggagg cacagcggcg gcaatcccga
     4981 ccctactttg taggggaggg cgcacttacc ggtttctctt cgagaaactg gcctaacggc
     5041 cacccttcgg gcggtgcgct ctccgagggc cattgcatgg agccgaaaag caaaagcaac
     5101 agcgaggcag catggcgatt tatcacctta cggcgaaaac cggcagcagg tcgggcggcc
     5161 aatcggccag ggccaaggcc gactacatcc agcgcgaagg caagtatgcc cgcgacatgg
     5221 atgaagtctt gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg cccgccgact
     5281 actgggatgc tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag gaggtcgaat
     5341 ttgccctgcc ggtcgagctg accctcgacc agcagaaggc gctggcgtcc gagttcgccc
     5401 agcacctgac cggtgccgag cgcctgccgt atacgctggc catccatgcc ggtggcggcg
     5461 agaacccgca ctgccacctg atgatctccg agcggatcaa tgacggcatc gagcggcccg
     5521 ccgctcagtg gttcaagcgg tacaacggca agaccccgga gaagggcggg gcacagaaga
     5581 ccgaagcgct caagcccaag gcatggcttg agcagacccg cgaggcatgg gccgaccatg
     5641 ccaaccgggc attagagcgg gctggccacg acgcccgcat tgaccacaga acacttgagg
     5701 cgcagggcat cgagcgcctg cccggtgttc acctggggcc gaacgtggtg gagatggaag
     5761 gccggggcat ccgcaccgac cgggcagacg tggccctgaa catcgacacc gccaacgccc
     5821 agatcatcga cttacaggaa taccgggagg caatagacca tgaacgcaat cgacagagtg
     5881 aagaaatcca gaggcatcaa cgagttagcg gagcagatcg aaccgctggc ccagagcatg
     5941 gcgacactgg ccgacgaagc ccggcaggtc atgagccaga cccagcaggc cagcgaggcg
     6001 caggcggcgg agtggctgaa agcccagcgc cagacagggg cggcatgggt ggagctggcc
     6061 aaagagttgc gggaggtagc cgccgaggtg agcagcgccg cgcagagcgc ccggagcgcg
     6121 tcgcgggggt ggcactggaa gctatggcta accgtgatgc tggcttccat gatgcctacg
     6181 gtggtgctgc tgatcgcatc gttgctcttg ctcgacctga cgccactgac aaccgaggac
     6241 ggctcgatct ggctgcgctt ggtggcccga tgaagaacga caggactttg caggccatag
     6301 gccgacagct caaggccatg ggctgtgagc gcttcgatat cggcgtcagg gacgccacca
     6361 ccggccagat gatgaaccgg gaatggtcag ccgccgaagt gctccagaac acgccatggc
     6421 tcaagcggat gaatgcccag ggcaatgacg tgtatatcag gcccgccgag caggagcggc
     6481 atggtctggt gctggtggac gacctcagcg agtttgacct ggatgacatg aaagccgagg
     6541 gccgggagcc tgccctggta gtggaaacca gcccgaagaa ctatcaggca tgggtcaagg
     6601 tggccgacgc cgcaggcggt gaacttcggg ggcagattgc ccggacgctg gccagcgagt
     6661 acgacgccga cccggccagc gccgacagcc gccactatgg ccgcttggcg ggcttcacca
     6721 accgcaagga caagcacacc acccgcgccg gttatcagcc gtgggtgctg ctgcgtgaat
     6781 ccaagggcaa gaccgccacc gctggcccgg cgctggtgca gcaggctggc cagcagatcg
     6841 agcaggccca gcggcagcag gagaaggccc gcaggctggc cagcctcgaa ctgcccgagc
     6901 ggcagcttag ccgccaccgg cgcacggcgc tggacgagta ccgcagcgag atggccgggc
     6961 tggtcaagcg cttcggtgat gacctcagca agtgcgactt tatcgccgcg cagaagctgg
     7021 ccagccgggg ccgcagtgcc gaggaaatcg gcaaggccat ggccgaggcc agcccagcgc
     7081 tggcagagcg caagcccggc cacgaagcgg attacatcga gcgcaccgtc agcaaggtca
     7141 tgggtctgcc cagcgtccag cttgcgcggg ccgagctggc acgggcaccg gcaccccgcc
     7201 agcgaggcat ggacaggggc gggccagatt tcagcatgta gtgcttgcgt tggtactcac
     7261 gcctgttata ctatgagtac tcacgcacag aagggggttt tatggaatac gaaaaaagcg
     7321 cttcagggtc ggtctacctg atcaaaagtg acaagggcta ttggttgccc ggtggctttg
     7381 gttatacgtc aaacaaggcc gaggctggcc gcttttcagt cgctgatatg gccagcctta
     7441 accttgacgg ctgcaccttg tccttgttcc gcgaagacaa gcctttcggc cccggcaagt
     7501 ttctcggtga ctgatatgaa agaccaaaag gacaagcaga ccggcgacct gctggccagc
     7561 cctgacgctg tacgccaagc gcgatatgcc gagcgcatga aggccaaagg gatgcgtcag
     7621 cgcaagttct ggctgaccga cgacgaatac gaggcgctgc gcgagtgcct ggaagaactc
     7681 agagcggcgc agggcggggg tagtgacccc gccagcgcct aaccaccaac tgcctgcaaa
     7741 ggaggcaatc aatggctacc cataagccta tcaatattct ggaggcgttc gcagcagcgc
     7801 cgccaccgct ggactacgtt ttgcccaaca tggtggccgg tacggtcggg gcgctggtgt
     7861 cgcccggtgg tgccggtaaa tccatgctgg ccctgcaact ggccgcacag attgcaggcg
     7921 ggccggatct gctggaggtg ggcgaactgc ccaccggccc ggtgatctac ctgcccgccg
     7981 aagacccgcc caccgccatt catcaccgcc tgcacgccct tggggcgcac ctcagcgccg
     8041 aggaacggca agccgtggct gacggcctgc tgatccagcc gctgatcggc agcctgccca
     8101 acatcatggc cccggagtgg ttcgacggcc tcaagcgcgc cgccgagggc cgccgcctga
     8161 tggtgctgga cacgctgcgc cggttccaca tcgaggaaga aaacgccagc ggccccatgg
     8221 cccaggtcat cggtcgcatg gaggccatcg ccgccgatac cgggtgctct atcgtgttcc
     8281 tgcaccatgc cagcaagggc gcggccatga tgggcgcagg cgaccagcag caggccagcc
     8341 ggggcagctc ggtactggtc gataacatcc gctggcagtc ctacctgtcg agcatgacca
     8401 gcgccgaggc cgaggaatgg ggtgtggacg acgaccagcg ccggttcttc gtccgcttcg
     8461 gtgtgagcaa ggccaactat ggcgcaccgt tcgctgatcg gtggttcagg cggcatgacg
     8521 gcggggtgct caagcccgcc gtgctggaga ggcagcgcaa gagcaagggg gtgccccgtg
     8581 gtgaagccta agaacaagca cagcctcagc cacgtccggc acgacccggc gcactgtctg
     8641 gcccccggcc tgttccgtgc cctcaagcgg ggcgagcgca agcgcagcaa gctggacgtg
     8701 acgtatgact acggcgacgg caagcggatc gagttcagcg gcccggagcc gctgggcgct
     8761 gatgatctgc gcatcctgca agggctggtg gccatggctg ggcctaatgg cctagtgctt
     8821 ggcccggaac ccaagaccga aggcggacgg cagctccggc tgttcctgga acccaagtgg
     8881 gaggccgtca ccgctgaatg ccatgtggtc aaaggtagct atcgggcgct ggcaaaggaa
     8941 atcggggcag aggtcgatag tggtggggcg ctcaagcaca tacaggactg catcgagcgc
     9001 ctttggaagg tatccatcat cgcccagaat ggccgcaagc ggcaggggtt tcggctgctg
     9061 tcggagtacg ccagcgacga ggcggacggg cgcctgtacg tggccctgaa ccccttgatc
     9121 gcgcaggccg tcatgggtgg cggccagcat gtgcgcatca gcatggacga ggtgcgggcg
     9181 ctggacagcg aaaccgcccg cctgctgcac cagcggctgt gtggctggat cgaccccggc
     9241 aaaaccggca aggcttccat agataccttg tgcggctatg tctggccgtc agaggccagt
     9301 ggttcgacca tgcgcaagcg ccgccagcgg gtgcgcgagg cgttgccgga gctggtcgcg
     9361 ctgggctgga cggtaaccga gttcgcggcg ggcaagtacg acatcacccg gcccaaggcg
     9421 gcaggctgac cccccccact ctattgtaaa caagacattt ttatctttta tattcaatgg
     9481 cttattttcc tgctaattgg taataccatg aaaaatacca tgctcagaaa aggcttaaca
     9541 atattttgaa aaattgccta ctgagcgctg ccgcacagct ccataggccg ctttcctggc
     9601 tttgcttcca gatgtatgct cttctgctcc tgcagctaat ggatcaccgc aaacaggtta
     9661 ctcgcctggg gattcccttt cgacccgagc atccgtatga tactcatgct cgattattat
     9721 tattatagaa gcccccatga ataaatcgct catcattttc ggcatcgtca acataacctc
     9781 ggacagtttc tccgatggag gccggtatct ggcgccagac gcagccattg cgcaggcgcg
     9841 taagctgatg gccgaggggg cagatgtgat cgacctggtc cggcatccag caatcccgac
     9901 gccgcgcctg tttcgtccga cacagaaatc gcgcgtatgc gccggtgctg gacgcgctca
     9961 ggcagatggc attcccgtct cgctcgacag ttatcaaccc gcgacgcaag cctatgcctt
    10021 gtcgcgtggt gtggcctatc tcaatgatat tcgcggtttt ccagacgctg cgttctatcc
    10081 gcaattggcg aaatcatctg ccaaactcgt cgttatgcat tcggtgcaag acgggcaggc
    10141 agatcggcgc gaggcacccg ctggcgacat catggatcac attgcggcgt tctttgacgc
    10201 gcgcatcgcg gcgctgacgg gtgccggtat caaacgcaac cgccttgtcc ttgatcccgg
    10261 catggggttt tttctggggg ctgctcccga aacctcgctc tcggtgctgg cgcggttcga
    10321 tgaattgcgg ctgcgcttcg atttgccggt gcttctgtct gtttcgcgca aatcctttct
    10381 gcgcgcgctc acaggccgtg gtccgggggt gtcggggccg cgacactcgc tgcagagctt
    10441 gccgccgccg caggtggagc tgacttcatc cgcacacacg agccgcgccc cttgcgcgac
    10501 gggctggcgg tattggcggc gctgaaagaa accgcaagaa ttcgtt
//
