LOCUS       62056_18680        9397 bp DNA     circular SYN 01-JAN-2019
DEFINITION  Cloning vector pQGS, complete sequence.
ACCESSION   MH423581
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Zhang X, Zheng H, Georgoulis SJ, Deatherage DE, Moran NA, Barrick 
            JE.
  TITLE     Evolution of small satellite plasmids stabilizes the maintenance of 
            newly acquired accessory genes in bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9397)
  AUTHORS   Zhang X.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2018) Molecular Biology, University of Texas at 
            Austin, 2500 Speedway A5000, Austin, TX 78712, USA
REFERENCE   3  (bases 1 to 9397)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2018) Molecular Biology, University of Texas at Austin, 2500
            Speedway A5000, Austin, TX 78712, USA"
FEATURES             Location/Qualifiers
     source          1..9397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(437..831)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(858..1142)
                     /codon_start=1
                     /transl_table=11
                     /product="mobC protein"
                     /label=mobC protein
                     /protein_id="AXG25720.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
     regulatory      1134..1164
                     /label=P1
                     /note="P1"
                     /regulatory_class="promoter"
     oriT            1173..1260
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             2089..2502
                     /codon_start=1
                     /transl_table=11
                     /product="mobB protein"
                     /label=mobB protein
                     /protein_id="AXG25725.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
     CDS             2499..3467
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     regulatory      3472..3503
                     /label=P4 promoter
                     /note="P4 promoter"
                     /regulatory_class="promoter"
     CDS             3531..3743
                     /codon_start=1
                     /transl_table=11
                     /product="repE protein"
                     /label=repE protein
                     /protein_id="AXG25727.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
     CDS             3745..3951
                     /codon_start=1
                     /transl_table=11
                     /product="repF protein"
                     /label=repF protein
                     /protein_id="AXG25719.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
     CDS             3981..4817
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             4807..5655
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     regulatory      5685..5818
                     /label=specR
                     /note="specR"
                     /regulatory_class="promoter"
     CDS             6038..6826
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     CDS             6961..8040
                     /label=lacI
                     /note="lac repressor"
     terminator      8063..8111
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     terminator      complement(8171..8214)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(8409..9122)
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
     regulatory      complement(9123..9218)
                     /label=gfp
                     /note="gfp"
                     /regulatory_class="promoter"
ORIGIN
        1 ctgtcagacc aagtttactc atatatactt tagattgatt tctgaaagcg accaggtgct
       61 cggcgtggca agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga
      121 gaaattcttc aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa
      181 gcgcagcgaa tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc
      241 ggtggctctg gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag
      301 gcatgttccg cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt
      361 cttttaccct cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact
      421 cccgtactaa ctgtcacgaa cccctgcaat aactgtcacg cccccctgca ataactgtca
      481 cgaacccctg caataactgt cacgccccca aacctgcaaa cccagcaggg gcgggggctg
      541 gcggggtgtt ggaaaaatcc atccatgatt atctaagaat aatccactag gcgcggttat
      601 cagcgccctt gtggggcgct gctgcccttg cccaatatgc ccggccagag gccggatagc
      661 tggtctattc gctgcgctag gctacacacc gccccaccgc tgcgcggcag ggggaaaggc
      721 gggcaaagcc cgctaaaccc cacaccaaac cccgcagaaa tacgctggag cgcttttagc
      781 cgctttagcg gcctttcccc ctacccgaag ggtgggggcg cgtgtgcagc cccgcagggc
      841 ctgtctcggt cgatcattca gcccggctca tccttctggc gtggcggcag accgaacaag
      901 gcgcggtcgt ggtcgcgttc aaggtacgca tccattgccg ccatgagccg atcctccggc
      961 cactcgctgc tgttcacctt ggccaaaatc atggccccca ccagcacctt gcgccttgtt
     1021 tcgttcttgc gctcttgctg ctgttccctt gcccgcaccc gctgaatttc ggcattgatt
     1081 cgcgctcgtt gttcttcgag cttggccagc cgatccgccg ccttgttgct ccccttaacc
     1141 atcttgacac cccattgtta atgtgctgtc tcgtaggcta tcatggaggc acagcggcgg
     1201 caatcccgac cctactttgt aggggagggc gcacttaccg gtttctcttc gagaaactgg
     1261 cctaacggcc acccttcggg cggtgcgctc tccgagggcc attgcatgga gccgaaaagc
     1321 aaaagcaaca gcgaggcagc atggcgattt atcaccttac ggcgaaaacc ggcagcaggt
     1381 cgggcggcca atcggccagg gccaaggccg actacatcca gcgcgaaggc aagtatgccc
     1441 gcgacatgga tgaagtcttg cacgccgaat ccgggcacat gccggagttc gtcgagcggc
     1501 ccgccgacta ctgggatgct gccgacctgt atgaacgcgc caatgggcgg ctgttcaagg
     1561 aggtcgaatt tgccctgccg gtcgagctga ccctcgacca gcagaaggcg ctggcgtccg
     1621 agttcgccca gcacctgacc ggtgccgagc gcctgccgta tacgctggcc atccatgccg
     1681 gtggcggcga gaacccgcac tgccacctga tgatctccga gcggatcaat gacggcatcg
     1741 agcggcccgc cgctcagtgg ttcaagcggt acaacggcaa gaccccggag aagggcgggg
     1801 cacagaagac cgaagcgctc aagcccaagg catggcttga gcagacccgc gaggcatggg
     1861 ccgaccatgc caaccgggca ttagagcggg ctggccacga cgcccgcatt gaccacagaa
     1921 cacttgaggc gcagggcatc gagcgcctgc ccggtgttca cctggggccg aacgtggtgg
     1981 agatggaagg ccggggcatc cgcaccgacc gggcagacgt ggccctgaac atcgacaccg
     2041 ccaacgccca gatcatcgac ttacaggaat accgggaggc aatagaccat gaacgcaatc
     2101 gacagagtga agaaatccag aggcatcaac gagttagcgg agcagatcga accgctggcc
     2161 cagagcatgg cgacactggc cgacgaagcc cggcaggtca tgagccagac ccagcaggcc
     2221 agcgaggcgc aggcggcgga gtggctgaaa gcccagcgcc agacaggggc ggcatgggtg
     2281 gagctggcca aagagttgcg ggaggtagcc gccgaggtga gcagcgccgc gcagagcgcc
     2341 cggagcgcgt cgcgggggtg gcactggaag ctatggctaa ccgtgatgct ggcttccatg
     2401 atgcctacgg tggtgctgct gatcgcatcg ttgctcttgc tcgacctgac gccactgaca
     2461 accgaggacg gctcgatctg gctgcgcttg gtggcccgat gaagaacgac aggactttgc
     2521 aggccatagg ccgacagctc aaggccatgg gctgtgagcg cttcgatatc ggcgtcaggg
     2581 acgccaccac cggccagatg atgaaccggg aatggtcagc cgccgaagtg ctccagaaca
     2641 cgccatggct caagcggatg aatgcccagg gcaatgacgt gtatatcagg cccgccgagc
     2701 aggagcggca tggtctggtg ctggtggacg acctcagcga gtttgacctg gatgacatga
     2761 aagccgaggg ccgggagcct gccctggtag tggaaaccag cccgaagaac tatcaggcat
     2821 gggtcaaggt ggccgacgcc gcaggcggtg aacttcgggg gcagattgcc cggacgctgg
     2881 ccagcgagta cgacgccgac ccggccagcg ccgacagccg ccactatggc cgcttggcgg
     2941 gcttcaccaa ccgcaaggac aagcacacca cccgcgccgg ttatcagccg tgggtgctgc
     3001 tgcgtgaatc caagggcaag accgccaccg ctggcccggc gctggtgcag caggctggcc
     3061 agcagatcga gcaggcccag cggcagcagg agaaggcccg caggctggcc agcctcgaac
     3121 tgcccgagcg gcagcttagc cgccaccggc gcacggcgct ggacgagtac cgcagcgaga
     3181 tggccgggct ggtcaagcgc ttcggtgatg acctcagcaa gtgcgacttt atcgccgcgc
     3241 agaagctggc cagccggggc cgcagtgccg aggaaatcgg caaggccatg gccgaggcca
     3301 gcccagcgct ggcagagcgc aagcccggcc acgaagcgga ttacatcgag cgcaccgtca
     3361 gcaaggtcat gggtctgccc agcgtccagc ttgcgcgggc cgagctggca cgggcaccgg
     3421 caccccgcca gcgaggcatg gacaggggcg ggccagattt cagcatgtag tgcttgcgtt
     3481 ggtactcacg cctgttatac tatgagtact cacgcacaga agggggtttt atggaatacg
     3541 aaaaaagcgc ttcagggtcg gtctacctga tcaaaagtga caagggctat tggttgcccg
     3601 gtggctttgg ttatacgtca aacaaggccg aggctggccg cttttcagtc gctgatatgg
     3661 ccagccttaa ccttgacggc tgcaccttgt ccttgttccg cgaagacaag cctttcggcc
     3721 ccggcaagtt tctcggtgac tgatatgaaa gaccaaaagg acaagcagac cggcgacctg
     3781 ctggccagcc ctgacgctgt acgccaagcg cgatatgccg agcgcatgaa ggccaaaggg
     3841 atgcgtcagc gcaagttctg gctgaccgac gacgaatacg aggcgctgcg cgagtgcctg
     3901 gaagaactca gagcggcgca gggcgggggt agtgaccccg ccagcgccta accaccaact
     3961 gcctgcaaag gaggcaatca atggctaccc ataagcctat caatattctg gaggcgttcg
     4021 cagcagcgcc gccaccgctg gactacgttt tgcccaacat ggtggccggt acggtcgggg
     4081 cgctggtgtc gcccggtggt gccggtaaat ccatgctggc cctgcaactg gccgcacaga
     4141 ttgcaggcgg gccggatctg ctggaggtgg gcgaactgcc caccggcccg gtgatctacc
     4201 tgcccgccga agacccgccc accgccattc atcaccgcct gcacgccctt ggggcgcacc
     4261 tcagcgccga ggaacggcaa gccgtggctg acggcctgct gatccagccg ctgatcggca
     4321 gcctgcccaa catcatggcc ccggagtggt tcgacggcct caagcgcgcc gccgagggcc
     4381 gccgcctgat ggtgctggac acgctgcgcc ggttccacat cgaggaagaa aacgccagcg
     4441 gccccatggc ccaggtcatc ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta
     4501 tcgtgttcct gcaccatgcc agcaagggcg cggccatgat gggcgcaggc gaccagcagc
     4561 aggccagccg gggcagctcg gtactggtcg ataacatccg ctggcagtcc tacctgtcga
     4621 gcatgaccag cgccgaggcc gaggaatggg gtgtggacga cgaccagcgc cggttcttcg
     4681 tccgcttcgg tgtgagcaag gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc
     4741 ggcatgacgg cggggtgctc aagcccgccg tgctggagag gcagcgcaag agcaaggggg
     4801 tgccccgtgg tgaagcctaa gaacaagcac agcctcagcc acgtccggca cgacccggcg
     4861 cactgtctgg cccccggcct gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag
     4921 ctggacgtga cgtatgacta cggcgacggc aagcggatcg agttcagcgg cccggagccg
     4981 ctgggcgctg atgatctgcg catcctgcaa gggctggtgg ccatggctgg gcctaatggc
     5041 ctagtgcttg gcccggaacc caagaccgaa ggcggacggc agctccggct gttcctggaa
     5101 cccaagtggg aggccgtcac cgctgatgcc atggtggtca aaggtagcta tcgggcgctg
     5161 gcaaaggaaa tcggggcaga ggtcgatagt ggtggggcgc tcaagcacat acaggactgc
     5221 atcgagcgcc tttggaaggt atccatcatc gcccagaatg gccgcaagcg gcaggggttt
     5281 cggctgctgt cggagtacgc cagcgacgag gcggacgggc gcctgtacgt ggccctgaac
     5341 cccttgatcg cgcaggccgt catgggtggc ggccagcatg tgcgcatcag catggacgag
     5401 gtgcgggcgc tggacagcga aaccgcccgc ctgctgcacc agcggctgtg tggctggatc
     5461 gaccccggca aaaccggcaa ggcttccata gataccttgt gcggctatgt ctggccgtca
     5521 gaggccagtg gttcgaccat gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag
     5581 ctggtcgcgc tgggctggac ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg
     5641 cccaaggcgg caggctgact cacgttaagg gattttggtc atgaccagcc aggacagaaa
     5701 tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     5761 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     5821 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     5881 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     5941 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6001 gcagcagggc agtcgcccta aaacaaagtt aaacattatg agggaagcgg tgatcgccga
     6061 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     6121 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     6181 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     6241 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     6301 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     6361 atttggagaa tggcagcgca atgacattct tgcgggtatc ttcgagccag ccacgatcga
     6421 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     6481 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     6541 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     6601 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     6661 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     6721 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     6781 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag cggccgctcg
     6841 gtatgcggta gtttattctt gacatgacga gacaggtgtg gtataatggg tctagattag
     6901 gatacagaaa cagaggagat attacggtac gcaagtacac aagatacagg agaggtactc
     6961 atgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
     7021 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
     7081 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
     7141 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
     7201 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
     7261 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
     7321 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
     7381 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
     7441 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
     7501 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
     7561 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
     7621 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
     7681 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
     7741 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
     7801 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
     7861 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccg
     7921 gtgtcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     7981 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     8041 tgatgtaaaa gcaagctgat aaaccgatac aattaaaggc tccttttgga gccttttttt
     8101 ttggagattt tcaacatgaa gaaattatta tttgatgatc agatagcggc ggggaactgc
     8161 cagacatcaa ataaaacaaa aggctcagtc ggaagactgg gccttttgtt ttatctgttg
     8221 tttgtcggtg aacactctcc cggttgtacc ctccatcgcc tcgcattctc ggatgtcctg
     8281 ggggttcgta acatctctgt aactgcttga gacgcagctg acaaatgctc tttccctaaa
     8341 ctccccccat aaaaaaaccc gccgaagcgg gtttttacgt tatttgcgga ttaacgatta
     8401 cggatttatt tgtatagttc atccatgccg tgtgtaatcc cagcagccgt gacaaactca
     8461 agaaggacca tgtggtcgcg cttttcgttg ggatctttcg aaagggcaga ttgagtggac
     8521 aggtaatggt tgtctggtaa cagcacaggc ccatcgccaa ttggggtgtt ttgttggtaa
     8581 tggtcggcta gttgaacgct tccatcctca atgttgtgtc tgatcttgaa gttaaccttg
     8641 ataccgttct tttgcttgtc ggccataatg tacacattat gggagttata gttatattcc
     8701 agtttgtggc ctaaaatatt tccatcttcc ttaaagtcaa taccctttaa ctcgattcta
     8761 ttaacaaggg tatcaccctc gaacttcact tcggcgcgtg tcttatagtt gccgtcatcc
     8821 ttaaaaaaga tggttctctc ctgtacatag ccttcgggca ttgcactctt gaaaaagtcg
     8881 tgctgtttca tgtgatctgg gtagcgcgca aagcactgaa ccccataccc gaacgtagtg
     8941 accagtgttg gccatggaac aggtagttta cccgtggtgc aaataaattt aagggtcagc
     9001 ttgccatatg ttgcgtcgcc ttcaccctct ccactgacag agaacttgtg cccattaaca
     9061 tcaccgtcta attccacgag gattgggaca actcctgtga aaagctcttc tcctttactc
     9121 atatgtaata tctcctctgt ttctgtattg actcactgtc gtttcatgtg gtacatcgta
     9181 ccgtgtttac ttcatgttgt caaccgtttt gtttcacccg ttgtattgcg acgaattgcc
     9241 acgttgtcgc agtgtctgta ctgtgggtta atgtctgtga tcgccgtcgt gagttcgtgt
     9301 catccgcgca tgattcagat ggtcctggag atcgttgctt gtaccggctg tttgtggctc
     9361 taccacgtct catcagtcta gatgcgaatt caggggc
//
