LOCUS       40924_36178        3494 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pQE30NST, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Bussow K, Cahill D, Nietfeld W, Bancroft D, Scherzinger E, Lehrach 
            H, Walter G.
  TITLE     A method for global protein expression and antibody screening on 
            high-density filters of an arrayed cDNA library
  JOURNAL   Nucleic Acids Res. 26 (21), 5007-5008 (1998)
  PUBMED    9776767
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Bussow K, Cahill D, Nietfeld W, Bancroft D, Scherzinger E, Lehrach 
            H, Walter G, Doebeli H, Eggimann B, Gentz R, Hochuli E, Stueber D.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-1998) Lehrach, Max-Planck-Institut fuer Molekulare
            Genetik, Ihnestrasse 73, Berlin 14195, Germany
REFERENCE   3  (bases 1 to 3494)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3494)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1998"; volume: "26"; issue: "21"; pages: 
            "5007-5008"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JUN-1998) Lehrach, Max-Planck-Institut fuer Molekulare Genetik, 
            Ihnestrasse 73, Berlin 14195, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             97..108
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     regulatory      101..107
                     /regulatory_class="ribosome_binding_site"
     misc_feature    115
                     /note="translation start site for inserts cloned into the 
                     multiple cloning site"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     promoter        152..170
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(201..219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      240..334
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             378..1034
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1102..1188
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1344..1484
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1670..2258)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2432..3289)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3290..3394)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc tatttaggtg acactataga atcgtcgacc
      181 tgcaagatct gcggccgctc cctatagtga gtcgtattaa gcttaattag ctgagcttgg
      241 actcctgttg atagatccag taatgacctc agaactccat ctggatttgt tcagaacgct
      301 cggttgccgc cgggcgtttt ttattggtga gaatccaagc tagcttggcg agattttcag
      361 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
      421 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
      481 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
      541 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaatttcgta
      601 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
      661 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
      721 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
      781 ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg agtttcacca
      841 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
      901 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
      961 tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     1021 ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggggtaa
     1081 tgactctcta gcttgaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     1141 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgctctag
     1201 agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     1261 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     1321 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     1381 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     1441 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
     1501 cgctcactga ctcgctgcgc tcggtctgtc ggctgcggcg agcggtatca gctcactcaa
     1561 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1621 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1681 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1741 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1801 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1861 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1921 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1981 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2041 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2101 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2161 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2221 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2281 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2341 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2401 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2461 cagcgatctg tctatttcgt tcatccatag ctgcctgact ccccgtcgtg tagataacta
     2521 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     2581 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2641 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     2701 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     2761 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     2821 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2881 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2941 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3001 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3061 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3121 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3181 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3241 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3301 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3361 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3421 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     3481 cctttcgtct tcac
//
