LOCUS       62056_18670        4298 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     CDS             127..144
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      1045..1139
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1183..1839
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1907..1993
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    2148..2288
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2474..3062)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3236..4093)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4094..4198)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatcgcatc accatcacca tcacggatcc tcgaaagcct ccattgggtt atttgtgcca
      181 gcaagtcctc ctctggaccc tgagaaggtc aaagagttac agcgcttcat caccctttcc
      241 aagagactcc ttgtgatgac tggggcagga atctccaccg aatcggggat accagactac
      301 aggtcagaaa aagtggggct ttatgcccgc actgaccgca ggcccatcca gcatggtgat
      361 tttgtccgga gtgccccaat ccgccagcgg tactgggcga gaaacttcgt aggctggcct
      421 caattctcct cccaccagcc taaccctgca cactgggctt tgagcacctg ggagaaactc
      481 ggaaagctgt actggttggt gacccaaaat gtggatgctt tgcacaccaa ggcggggagt
      541 cggcgcctga cagagctcca cggatgcatg gacagggtcc tgtgcttgga ttgtggggaa
      601 cagactcccc ggggggtgct gcaagagcgt ttccaagtcc tgaaccccac ctggagtgct
      661 gaggcccatg gcctggctcc tgatggtgac gtctttctct cagaggagca agtccggagc
      721 tttcaggtcc caacctgcgt tcaatgtgga ggccatctga aaccagatgt cgttttcttc
      781 ggggacacag tgaaccctga caaggttgat tttgtgcaca agcgtgtaaa agaagccgac
      841 tccctcttgg tggtgggatc atccttgcag gtatactctg gttacaggtt tatcctcact
      901 gcctgggaga agaagctccc gattgcaata ctgaacattg ggcccacacg gtcggatgac
      961 ttggcgtgtc tgaaactgaa ttctcgttgt ggagagttgc tgcctttgat agacccatgc
     1021 tgaaagctta attagctgag cttggactcc tgttgataga tccagtaatg acctcagaac
     1081 tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc
     1141 caagctagct tggcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     1201 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     1261 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     1321 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     1381 atgaatgctc atccggaatt tcgtatggca atgaaagacg gtgagctggt gatatgggat
     1441 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     1501 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     1561 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1621 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1681 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1741 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1801 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
     1861 tggtgccctt aaacgcctgg ggtaatgact ctctagcttg aggcatcaaa taaaacgaaa
     1921 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
     1981 gagtaggaca aatccgccct ctagagctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     2041 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     2101 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     2161 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
     2221 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     2281 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2341 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2401 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2461 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2521 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2581 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2641 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2701 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2761 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     2821 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2881 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     2941 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3001 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3061 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3121 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3181 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3241 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3301 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3361 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3421 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     3481 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     3541 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     3601 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     3661 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     3721 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     3781 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     3841 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     3901 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3961 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4021 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4081 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4141 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4201 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     4261 ataaaaatag gcgtatcacg aggccctttc gtcttcac
//
