LOCUS       pQE-TriSystem.        5766 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expression of C-terminally 8xHis-tagged proteins in 
            bacterial, insect, and vertebrate cells.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-TriSystem
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5766)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5766)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        64..367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        373..650
                     /label=chicken beta-actin promoter
     exon            651..742
                     /note="Exon 1"
     intron          743..1004
     promoter        1073..1116
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     promoter        1143..1252
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     RBS             1255..1260
                     /note="ribosome binding site"
     misc_feature    1265..1372
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1267..1269
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1378..1410
                     /label=HSV tag
                     /note="HSV (herpes simplex virus) epitope tag"
     CDS             1417..1440
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_feature    1441..1451
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    1595..1650
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     terminator      1738..1785
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     1798..3256
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3415..4002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4018..4875)
                     /label=AmpR
                     /note="beta-lactamase"
     misc_recomb     4941..5766
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
ORIGIN
        1 ctctagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
       61 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      361 taccatgcat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
      421 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
      481 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
      541 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
      601 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgtgc
      661 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      721 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctt cgggctgtaa
      781 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      841 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
      901 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
      961 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1021 tggttattgt gctgtctcat cattttggca aagaattgga tcggaccgga aatcataaaa
     1081 aattatttgc tttgtgagcg gataacaatt ataatagatt caattgaggc ctcgaccacc
     1141 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
     1201 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaaaggaga
     1261 tataccatgg cgatatcccg ggagctcgtg gatccgaatt ctcagatctc ggcgcgcctg
     1321 caggtcgacg gtaccggttc gaagcttgcg gccgcacagc tgtatacacg tgcaagccag
     1381 ccagaactcg ccccggaaga ccccgaggat ctcgagcacc accatcacca tcaccatcac
     1441 taagtgatta acctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg
     1501 ccctggctca caaataccac tgagatcgat ctttttccct ctgccaaaaa ttatggggac
     1561 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     1621 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     1681 ttaaaacatc agaatgagtt tttggtttag agtttggcaa catatgccca tatgtaacta
     1741 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa agcatgcgga
     1801 ggaaattctc cttgaagttt ccctggtgtt caaagtaaag gagtttgcac cagacgcacc
     1861 tctgttcact ggtccggcgt attaaaacac gatacattgt tattagtaca tttattaagc
     1921 gctagattct gtgcgttgtt gatttacaga caattgttgt acgtatttta ataattcatt
     1981 aaatttataa tctttagggt ggtatgttag agcgaaaatc aaatgatttt cagcgtcttt
     2041 atatctgaat ttaaatatta aatcctcaat agatttgtaa aataggtttc gattagtttc
     2101 aaacaagggt tgtttttccg aaccgatggc tggactatct aatggatttt cgctcaacgc
     2161 cacaaaactt gccaaatctt gtagcagcaa tctagctttg tcgatattcg tttgtgtttt
     2221 gttttgtaat aaaggttcga cgtcgttcaa aatattatgc gcttttgtat ttctttcatc
     2281 actgtcgtta gtgtacaatt gactcgacgt aaacacgtta aatagagctt ggacatattt
     2341 aacatcgggc gtgttagctt tattaggccg attatcgtcg tcgtcccaac cctcgtcgtt
     2401 agaagttgct tccgaagacg attttgccat agccacacga cgcctattaa ttgtgtcggc
     2461 taacacgtcc gcgatcaaat ttgtagttga gctttttgga attatttctg attgcgggcg
     2521 tttttgggcg ggtttcaatc taactgtgcc cgattttaat tcagacaaca cgttagaaag
     2581 cgatggtgca ggcggtggta acatttcaga cggcaaatct actaatggcg gcggtggtgg
     2641 agctgatgat aaatctacca tcggtggagg cgcaggcggg gctggcggcg gaggcggagg
     2701 cggaggtggt ggcggtgatg cagacggcgg tttaggctca aatgtctctt taggcaacac
     2761 agtcggcacc tcaactattg tactggtttc gggcgccgtt tttggtttga ccggtctgag
     2821 acgagtgcga tttttttcgt ttctaatagc ttccaacaat tgttgtctgt cgtctaaagg
     2881 tgcagcgggt tgaggttccg tcggcattgg tggagcgggc ggcaattcag acatcgatgg
     2941 tggtggtggt ggtggaggcg ctggaatgtt aggcacggga gaaggtggtg gcggcggtgc
     3001 cgccggtata atttgttctg gtttagtttg ttcgcgcacg attgtgggca ccggcgcagg
     3061 cgccgctggc tgcacaacgg aaggtcgtct gcttcgaggc agcgcttggg gtggtggcaa
     3121 ttcaatatta taattggaat acaaatcgta aaaatctgct ataagcattg taatttcgct
     3181 atcgtttacc gtgccgatat ttaacaaccg ctcaatgtaa gcaattgtat tgtaaagaga
     3241 ttgtctcaag ctcggaacgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     3301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3421 taggctccgc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     3481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     3541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     3601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     3661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     3721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     3781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     3841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     3901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     3961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgttacca
     4021 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     4081 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     4141 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4201 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4261 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4321 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4381 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4441 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4501 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4561 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4621 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4681 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4741 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4801 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4861 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4921 tctcatgtcc gcgcgtttcc tgcatctttt aatcaaatcc caagatgtgt ataaaccacc
     4981 aaactgccaa aaaatgaaaa ctgtcgacaa gctctgtccg tttgctggca actgcaaggg
     5041 tctcaatcct atttgtaatt attgaataat aaaacaatta taaatgtcaa atttgttttt
     5101 tattaacgat acaaaccaaa cgcaacaaga acatttgtag tattatctat aattgaaaac
     5161 gcgtagttat aatcgctgag gtaatattta aaatcatttt caaatgattc acagttaatt
     5221 tgcgacaata taattttatt ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat
     5281 tccttctctt tttcattttt ctcttcataa aaattaacat agttattatc gtatccatat
     5341 atgtatctat cgtatagagt aaattttttg ttgtcataaa tatatatgtc ttttttaatg
     5401 gggtgtatag taccgctgcg catagttttt ctgtaattta caacagtgct attttctggt
     5461 agttcttcgg agtgtgttgc tttaattatt aaatttatat aatcaatgaa tttgggatcg
     5521 tcggttttgt acaatatgtt gccggcatag tacgcagctt cttctagttc aattacacca
     5581 ttttttagca gcaccggatt aacataactt tccaaaatgt tgtacgaacc gttaaacaaa
     5641 aacagttcac ctcccttttc tatactattg tctgcgagca gttgtttgtt gttaaaaata
     5701 acagccattg taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata
     5761 tagttg
//
