LOCUS       pQE-TriSystem_St        5736 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expression in bacterial, insect, and vertebrate cells of 
            proteins with a C-terminal Strep-Tag II tag.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-TriSystem Strep
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5736)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        64..367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        373..650
                     /label=chicken beta-actin promoter
     exon            651..742
                     /note="Exon 1"
     intron          743..1004
     promoter        1073..1116
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     promoter        1143..1252
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     RBS             1255..1260
                     /note="ribosome binding site"
     misc_feature    1265..1372
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1267..1269
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1381..1404
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     misc_feature    1411..1421
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    1565..1620
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     terminator      1708..1755
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     1768..3226
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3385..3972)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3988..4845)
                     /label=AmpR
                     /note="beta-lactamase"
     misc_recomb     4911..5736
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
ORIGIN
        1 ctctagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
       61 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      361 taccatgcat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
      421 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
      481 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
      541 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
      601 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgtgc
      661 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      721 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctt cgggctgtaa
      781 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      841 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
      901 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
      961 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1021 tggttattgt gctgtctcat cattttggca aagaattgga tcggaccgga aatcataaaa
     1081 aattatttgc tttgtgagcg gataacaatt ataatagatt caattgaggc ctcgaccacc
     1141 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
     1201 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaaaggaga
     1261 tataccatgg cgatatcccg ggagctcgtg gatccgaatt ctcagatctc ggcgcgcctg
     1321 caggtcgacg gtaccggttc gaagcttgcg gccgcacagc tgtatacacg tgcaagcgcg
     1381 tggagccacc cgcagttcga aaaataacac taagtgatta acctcaggtg caggctgcct
     1441 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcgat
     1501 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     1561 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     1621 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagtt tttggtttag
     1681 agtttggcaa catatgccca tatgtaacta gcataacccc ttggggcctc taaacgggtc
     1741 ttgaggggtt ttttgctgaa agcatgcgga ggaaattctc cttgaagttt ccctggtgtt
     1801 caaagtaaag gagtttgcac cagacgcacc tctgttcact ggtccggcgt attaaaacac
     1861 gatacattgt tattagtaca tttattaagc gctagattct gtgcgttgtt gatttacaga
     1921 caattgttgt acgtatttta ataattcatt aaatttataa tctttagggt ggtatgttag
     1981 agcgaaaatc aaatgatttt cagcgtcttt atatctgaat ttaaatatta aatcctcaat
     2041 agatttgtaa aataggtttc gattagtttc aaacaagggt tgtttttccg aaccgatggc
     2101 tggactatct aatggatttt cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa
     2161 tctagctttg tcgatattcg tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa
     2221 aatattatgc gcttttgtat ttctttcatc actgtcgtta gtgtacaatt gactcgacgt
     2281 aaacacgtta aatagagctt ggacatattt aacatcgggc gtgttagctt tattaggccg
     2341 attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct tccgaagacg attttgccat
     2401 agccacacga cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga
     2461 gctttttgga attatttctg attgcgggcg tttttgggcg ggtttcaatc taactgtgcc
     2521 cgattttaat tcagacaaca cgttagaaag cgatggtgca ggcggtggta acatttcaga
     2581 cggcaaatct actaatggcg gcggtggtgg agctgatgat aaatctacca tcggtggagg
     2641 cgcaggcggg gctggcggcg gaggcggagg cggaggtggt ggcggtgatg cagacggcgg
     2701 tttaggctca aatgtctctt taggcaacac agtcggcacc tcaactattg tactggtttc
     2761 gggcgccgtt tttggtttga ccggtctgag acgagtgcga tttttttcgt ttctaatagc
     2821 ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt tgaggttccg tcggcattgg
     2881 tggagcgggc ggcaattcag acatcgatgg tggtggtggt ggtggaggcg ctggaatgtt
     2941 aggcacggga gaaggtggtg gcggcggtgc cgccggtata atttgttctg gtttagtttg
     3001 ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc tgcacaacgg aaggtcgtct
     3061 gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta taattggaat acaaatcgta
     3121 aaaatctgct ataagcattg taatttcgct atcgtttacc gtgccgatat ttaacaaccg
     3181 ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag ctcggaacgc tgcgctcggt
     3241 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3301 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3361 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc cccctgacga gcatcacaaa
     3421 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3481 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3541 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3601 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3661 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3721 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3781 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3841 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3901 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3961 aaaggatctc aagaagatcc tttgttacca atgcttaatc agtgaggcac ctatctcagc
     4021 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4081 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4141 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4201 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4261 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4321 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4381 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4441 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4501 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4561 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4621 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4681 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4741 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4801 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4861 atattattga agcatttatc agggttattg tctcatgtcc gcgcgtttcc tgcatctttt
     4921 aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa ctgtcgacaa
     4981 gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt attgaataat
     5041 aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa cgcaacaaga
     5101 acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag gtaatattta
     5161 aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt ttcacataaa
     5221 ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt ctcttcataa
     5281 aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt aaattttttg
     5341 ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg catagttttt
     5401 ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc tttaattatt
     5461 aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt gccggcatag
     5521 tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt aacataactt
     5581 tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc tatactattg
     5641 tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg cacaaactaa
     5701 tatcacaaac tggaaatgtc tatcaatata tagttg
//
