LOCUS       pQE-TriSystem_Hi        5796 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expression in bacterial, insect, and vertebrate cells of 
            proteins with an N-terminal Strep-Tag II tag and a C-terminal 8xHis 
            tag.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-TriSystem His-Strep2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        64..367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        373..650
                     /label=chicken beta-actin promoter
     exon            651..742
                     /note="Exon 1"
     intron          743..1004
     promoter        1073..1116
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     promoter        1143..1252
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     RBS             1255..1260
                     /note="ribosome binding site"
     CDS             1267..1269
                     /codon_start=1
                     /label=ATG
                     /note="ATG"
                     /note="start codon"
                     /translation="M"
     CDS             1279..1302
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             1408..1440
                     /label=HSV tag
                     /note="HSV (herpes simplex virus) epitope tag"
     CDS             1447..1470
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_feature    1471..1481
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    1625..1680
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     terminator      1768..1815
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     1828..3286
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3445..4032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4048..4905)
                     /label=AmpR
                     /note="beta-lactamase"
     misc_recomb     4971..5796
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
ORIGIN
        1 ctctagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
       61 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      361 taccatgcat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
      421 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
      481 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
      541 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
      601 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgtgc
      661 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      721 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctt cgggctgtaa
      781 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      841 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
      901 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
      961 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1021 tggttattgt gctgtctcat cattttggca aagaattgga tcggaccgga aatcataaaa
     1081 aattatttgc tttgtgagcg gataacaatt ataatagatt caattgaggc ctcgaccacc
     1141 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
     1201 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaaaggaga
     1261 tataccatgg cgagcgcgtg gagccacccg cagttcgaaa aaatatcccg ggagctcgtg
     1321 gatccgaatt ctcagatctc ggcgcgcctg caggtcgacg gtaccggttc gaagcttgcg
     1381 gccgcacagc tgtatacacg tgcaagccag ccagaactcg ccccggaaga ccccgaggat
     1441 ctcgagcacc accatcacca tcaccatcac taagtgatta acctcaggtg caggctgcct
     1501 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcgat
     1561 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     1621 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     1681 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagtt tttggtttag
     1741 agtttggcaa catatgccca tatgtaacta gcataacccc ttggggcctc taaacgggtc
     1801 ttgaggggtt ttttgctgaa agcatgcgga ggaaattctc cttgaagttt ccctggtgtt
     1861 caaagtaaag gagtttgcac cagacgcacc tctgttcact ggtccggcgt attaaaacac
     1921 gatacattgt tattagtaca tttattaagc gctagattct gtgcgttgtt gatttacaga
     1981 caattgttgt acgtatttta ataattcatt aaatttataa tctttagggt ggtatgttag
     2041 agcgaaaatc aaatgatttt cagcgtcttt atatctgaat ttaaatatta aatcctcaat
     2101 agatttgtaa aataggtttc gattagtttc aaacaagggt tgtttttccg aaccgatggc
     2161 tggactatct aatggatttt cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa
     2221 tctagctttg tcgatattcg tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa
     2281 aatattatgc gcttttgtat ttctttcatc actgtcgtta gtgtacaatt gactcgacgt
     2341 aaacacgtta aatagagctt ggacatattt aacatcgggc gtgttagctt tattaggccg
     2401 attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct tccgaagacg attttgccat
     2461 agccacacga cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga
     2521 gctttttgga attatttctg attgcgggcg tttttgggcg ggtttcaatc taactgtgcc
     2581 cgattttaat tcagacaaca cgttagaaag cgatggtgca ggcggtggta acatttcaga
     2641 cggcaaatct actaatggcg gcggtggtgg agctgatgat aaatctacca tcggtggagg
     2701 cgcaggcggg gctggcggcg gaggcggagg cggaggtggt ggcggtgatg cagacggcgg
     2761 tttaggctca aatgtctctt taggcaacac agtcggcacc tcaactattg tactggtttc
     2821 gggcgccgtt tttggtttga ccggtctgag acgagtgcga tttttttcgt ttctaatagc
     2881 ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt tgaggttccg tcggcattgg
     2941 tggagcgggc ggcaattcag acatcgatgg tggtggtggt ggtggaggcg ctggaatgtt
     3001 aggcacggga gaaggtggtg gcggcggtgc cgccggtata atttgttctg gtttagtttg
     3061 ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc tgcacaacgg aaggtcgtct
     3121 gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta taattggaat acaaatcgta
     3181 aaaatctgct ataagcattg taatttcgct atcgtttacc gtgccgatat ttaacaaccg
     3241 ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag ctcggaacgc tgcgctcggt
     3301 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3361 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3421 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc cccctgacga gcatcacaaa
     3481 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3541 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3601 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3661 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3721 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3781 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3841 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     3901 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3961 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4021 aaaggatctc aagaagatcc tttgttacca atgcttaatc agtgaggcac ctatctcagc
     4081 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     4141 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     4201 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     4261 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     4321 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     4381 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4441 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4501 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4561 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4621 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4681 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4741 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4801 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4861 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4921 atattattga agcatttatc agggttattg tctcatgtcc gcgcgtttcc tgcatctttt
     4981 aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa ctgtcgacaa
     5041 gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt attgaataat
     5101 aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa cgcaacaaga
     5161 acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag gtaatattta
     5221 aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt ttcacataaa
     5281 ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt ctcttcataa
     5341 aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt aaattttttg
     5401 ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg catagttttt
     5461 ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc tttaattatt
     5521 aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt gccggcatag
     5581 tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt aacataactt
     5641 tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc tatactattg
     5701 tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg cacaaactaa
     5761 tatcacaaac tggaaatgtc tatcaatata tagttg
//
