LOCUS       pQE-TriSystem_Hi        5760 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expression in bacterial, insect, and vertebrate cells of 
            proteins with C-terminal Strep-Tag II and 8xHis tags.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-TriSystem His-Strep1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        64..367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        373..650
                     /label=chicken beta-actin promoter
     exon            651..742
                     /note="Exon 1"
     intron          743..1004
     promoter        1073..1116
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     promoter        1143..1252
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     RBS             1255..1260
                     /note="ribosome binding site"
     misc_feature    1265..1372
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1267..1269
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             1381..1404
                     /label=Strep-Tag II
                     /note="peptide that binds Strep-Tactin(R), an engineered 
                     form
                     of streptavidin"
     CDS             1411..1434
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_feature    1435..1445
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    1589..1644
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     terminator      1732..1779
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_recomb     1792..3250
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     rep_origin      complement(3409..3996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4012..4869)
                     /label=AmpR
                     /note="beta-lactamase"
     misc_recomb     4935..5760
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
ORIGIN
        1 ctctagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
       61 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      361 taccatgcat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
      421 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
      481 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
      541 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
      601 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgtgc
      661 gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg
      721 actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctt cgggctgtaa
      781 ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg
      841 gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct
      901 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
      961 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     1021 tggttattgt gctgtctcat cattttggca aagaattgga tcggaccgga aatcataaaa
     1081 aattatttgc tttgtgagcg gataacaatt ataatagatt caattgaggc ctcgaccacc
     1141 gggaccttta attcaaccca acacaatata ttatagttaa ataagaatta ttatcaaatc
     1201 atttgtatat taattaaaat actatactgt aaattacatt ttatttacaa tcaaaggaga
     1261 tataccatgg cgatatcccg ggagctcgtg gatccgaatt ctcagatctc ggcgcgcctg
     1321 caggtcgacg gtaccggttc gaagcttgcg gccgcacagc tgtatacacg tgcaagcgcg
     1381 tggagccacc cgcagttcga aaaactcgag caccaccatc accatcacca tcactaagtg
     1441 attaacctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg
     1501 ctcacaaata ccactgagat cgatcttttt ccctctgcca aaaattatgg ggacatcatg
     1561 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     1621 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     1681 catcagaatg agtttttggt ttagagtttg gcaacatatg cccatatgta actagcataa
     1741 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaagcatg cggaggaaat
     1801 tctccttgaa gtttccctgg tgttcaaagt aaaggagttt gcaccagacg cacctctgtt
     1861 cactggtccg gcgtattaaa acacgataca ttgttattag tacatttatt aagcgctaga
     1921 ttctgtgcgt tgttgattta cagacaattg ttgtacgtat tttaataatt cattaaattt
     1981 ataatcttta gggtggtatg ttagagcgaa aatcaaatga ttttcagcgt ctttatatct
     2041 gaatttaaat attaaatcct caatagattt gtaaaatagg tttcgattag tttcaaacaa
     2101 gggttgtttt tccgaaccga tggctggact atctaatgga ttttcgctca acgccacaaa
     2161 acttgccaaa tcttgtagca gcaatctagc tttgtcgata ttcgtttgtg ttttgttttg
     2221 taataaaggt tcgacgtcgt tcaaaatatt atgcgctttt gtatttcttt catcactgtc
     2281 gttagtgtac aattgactcg acgtaaacac gttaaataga gcttggacat atttaacatc
     2341 gggcgtgtta gctttattag gccgattatc gtcgtcgtcc caaccctcgt cgttagaagt
     2401 tgcttccgaa gacgattttg ccatagccac acgacgccta ttaattgtgt cggctaacac
     2461 gtccgcgatc aaatttgtag ttgagctttt tggaattatt tctgattgcg ggcgtttttg
     2521 ggcgggtttc aatctaactg tgcccgattt taattcagac aacacgttag aaagcgatgg
     2581 tgcaggcggt ggtaacattt cagacggcaa atctactaat ggcggcggtg gtggagctga
     2641 tgataaatct accatcggtg gaggcgcagg cggggctggc ggcggaggcg gaggcggagg
     2701 tggtggcggt gatgcagacg gcggtttagg ctcaaatgtc tctttaggca acacagtcgg
     2761 cacctcaact attgtactgg tttcgggcgc cgtttttggt ttgaccggtc tgagacgagt
     2821 gcgatttttt tcgtttctaa tagcttccaa caattgttgt ctgtcgtcta aaggtgcagc
     2881 gggttgaggt tccgtcggca ttggtggagc gggcggcaat tcagacatcg atggtggtgg
     2941 tggtggtgga ggcgctggaa tgttaggcac gggagaaggt ggtggcggcg gtgccgccgg
     3001 tataatttgt tctggtttag tttgttcgcg cacgattgtg ggcaccggcg caggcgccgc
     3061 tggctgcaca acggaaggtc gtctgcttcg aggcagcgct tggggtggtg gcaattcaat
     3121 attataattg gaatacaaat cgtaaaaatc tgctataagc attgtaattt cgctatcgtt
     3181 taccgtgccg atatttaaca accgctcaat gtaagcaatt gtattgtaaa gagattgtct
     3241 caagctcgga acgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3301 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3361 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3421 ccgccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3481 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3541 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3601 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3661 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3721 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3781 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3841 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3901 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3961 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgtt accaatgctt
     4021 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4081 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4141 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4201 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4261 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4321 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4381 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4441 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4501 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4561 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4621 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4681 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     4741 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     4801 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     4861 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     4921 gtccgcgcgt ttcctgcatc ttttaatcaa atcccaagat gtgtataaac caccaaactg
     4981 ccaaaaaatg aaaactgtcg acaagctctg tccgtttgct ggcaactgca agggtctcaa
     5041 tcctatttgt aattattgaa taataaaaca attataaatg tcaaatttgt tttttattaa
     5101 cgatacaaac caaacgcaac aagaacattt gtagtattat ctataattga aaacgcgtag
     5161 ttataatcgc tgaggtaata tttaaaatca ttttcaaatg attcacagtt aatttgcgac
     5221 aatataattt tattttcaca taaactagac gccttgtcgt cttcttcttc gtattccttc
     5281 tctttttcat ttttctcttc ataaaaatta acatagttat tatcgtatcc atatatgtat
     5341 ctatcgtata gagtaaattt tttgttgtca taaatatata tgtctttttt aatggggtgt
     5401 atagtaccgc tgcgcatagt ttttctgtaa tttacaacag tgctattttc tggtagttct
     5461 tcggagtgtg ttgctttaat tattaaattt atataatcaa tgaatttggg atcgtcggtt
     5521 ttgtacaata tgttgccggc atagtacgca gcttcttcta gttcaattac accatttttt
     5581 agcagcaccg gattaacata actttccaaa atgttgtacg aaccgttaaa caaaaacagt
     5641 tcacctccct tttctatact attgtctgcg agcagttgtt tgttgttaaa aataacagcc
     5701 attgtaatga gacgcacaaa ctaatatcac aaactggaaa tgtctatcaa tatatagttg
//
