LOCUS       pQE-T7-2.        5291 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing C-terminally 10xHis-tagged proteins in 
            bacteria using the QIAgenes system.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-T7-2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     A QIAgene can be cloned into this vector as an NdeI-XhoI fragment.
FEATURES             Location/Qualifiers
     source          1..5291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    137..142
                     /label=stop codons
                     /note="stop codons"
                     /note="two tandem stop codons"
     CDS             complement(146..172)
                     /label=9xHis
                     /note="9xHis affinity tag"
     misc_feature    173..224
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             complement(219..221)
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     RBS             complement(232..254)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(269..293)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(294..312)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        621..698
                     /label=lacI promoter
     CDS             699..1778
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1794..1815
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             2590..2778
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2883..3022
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3208..3796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             3918..4730
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(4825..5280)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatcttac taatgatgat ggtggtgatg gtgatgatga tgctcgaggg
      181 ctgcagacgt gggtacccgc ggccgcatga gctccatgca tatgatttat atctccttct
      241 taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat tcccctatag
      301 tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc
      361 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
      421 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
      481 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
      541 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
      601 gggagagcgt cgagatcccg gacaccatcg aatggcgcaa aacctttcgc ggtatggcat
      661 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg
      721 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
      781 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
      841 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
      901 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
      961 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     1021 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     1081 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     1141 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     1201 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     1261 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     1321 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     1381 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     1441 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     1501 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     1561 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     1621 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     1681 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     1741 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgta
     1801 agttagctca ctcattaggc accgggatct cgaccgatgc ccttgagagc cttcaaccca
     1861 gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc
     1921 tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac
     1981 cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac
     2041 gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc
     2101 attatcgccg gcatggcggc cccacgggtg cgcatgatcg tgctcctgtc gttgaggacc
     2161 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
     2221 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
     2281 ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc
     2341 cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
     2401 aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt
     2461 tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg
     2521 agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg
     2581 gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
     2641 cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
     2701 tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt
     2761 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     2821 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     2881 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     2941 catcagagca gattgtactg agagtgcacc attgcggtgt gaaataccgc acagatgcgt
     3001 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3061 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3121 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     3661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3721 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3841 cgaaaactca cgttaaggga ttttggtcat gaacaataaa actgtctgct tacataaaca
     3901 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctct aggccgcgat
     3961 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     4021 aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga
     4081 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     4141 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     4201 ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg
     4261 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     4321 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     4381 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     4441 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     4501 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
     4561 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
     4621 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
     4681 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taagaattaa
     4741 ttcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4801 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     4861 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     4921 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     4981 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     5041 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     5101 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     5161 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     5221 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     5281 cccattcgcc a
//
