LOCUS       62056_18665        4780 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             91..108
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      131..225
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             269..925
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPSFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      993..1079
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1234..1374
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1560..2148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2322..3179)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3180..3284)
                     /label=AmpR promoter
     promoter        3394..3438
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     CDS             4742..4759
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
ORIGIN
        1 ggcgggggtc gagcgacgcc agaccagaag tggcacctga ccgagcacgc catcgcctcc
       61 ggctccgcct tgcccggatc caaaggtgga catcatcatc atcatcatta agcttaatta
      121 gctgagcttg gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg
      181 ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaatccaag ctagcttggc
      241 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
      301 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
      361 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
      421 taagcacaag ttttatccgt cctttattca cattcttgcc cgcctgatga atgctcatcc
      481 ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
      541 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
      601 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
      661 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
      721 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
      781 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
      841 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
      901 ctgcgatgag tggcagggcg gggcgtaatt tttttaagtc agttattggt gcccttaaac
      961 gcctggggta atgactctct agcttgaggc atcaaataaa acgaaaggct cagtcgaaag
     1021 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     1081 cgccctctag agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     1141 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     1201 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
     1261 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     1321 catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct
     1381 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     1441 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1501 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     1561 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     1621 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     1681 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     1741 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     1801 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     1861 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1921 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1981 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     2041 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     2101 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     2161 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     2221 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2281 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2341 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2401 tagataacta caatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2461 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2521 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2581 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2641 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     2701 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     2761 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     2821 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     2881 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2941 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3001 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3061 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3121 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3181 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3241 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3301 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     3361 atcacgaggc cctttcgtct tcacctcgag aaatcataaa aaatttattt gctttgtgag
     3421 cggataacaa ttataataga ttcaattgtg agcggataac aatttcacac agaattcatt
     3481 aaagaggaga aattaaccat gggatccaga atgacacacg acgaatcccg ggatgcgcga
     3541 ctcccataac aacaccggtt tttccacacg ggccattcac cacggctacg acccgctttc
     3601 ccacggtggt gccttggtgc caccggtgta ccagaccgcg acctatgcct tcccgactgt
     3661 cgaatacggc gctgcgtgct tcgccgggga ggaggcgggg cacttctaca gccgcatctc
     3721 caaccccacc ctggccttgc tcgagcaacg catggcctcg ttggagggtg gtgaggcggg
     3781 attggcgctg gcgtcgggga tgggagccat tacttcgacc ctctggaccc tgctgcggcc
     3841 tggtgatgag ctgatcgtgg ggcgcacctt gtatggctgc acctttgcgt tcctgcacca
     3901 tggcattggc gagttcgggg tcaagatcca ccatgtcgac cttaacgatg ccaaggccct
     3961 gaaagcggcg atcaacagca aaacgcggat gatctacttc gaaacaccgg ccaaccccaa
     4021 catgcaactg gtggatatag cggcggtcgt cgaggcagtg cgggggagtg atgtgcttgt
     4081 ggtggtcgac aacacctact gcacgcccta cctgcagcgg ccactggaac tgggggcaga
     4141 cctggtggtg cattcggcaa ccaagtacct cagtggccat ggcgacatca ctgcgggcct
     4201 ggtggtgggg cgcaaggctt tggtcgaccg cattcggctg gaagggctga aagacatgac
     4261 cggggcagcc ttgtcaccgc atgacgctgc gttgttgatg cgcggcatca agaccctggc
     4321 gctgcgcatg gaccggcatt gcgccaacgc cctggaggtc gcgcagttcc tggccgggca
     4381 gccccaggtg gagctgatcc actacccggg cttgccgtcg tttgcccagt acgaactggc
     4441 acagcggcag atgcgtttgc cgggcgggat gattgccttt gagctcaagg gcggtatcga
     4501 ggccgggcgc ggcttcatga atgccctgca gctttttgcc cgtgcggtga gcctggggga
     4561 tgccgagtcg ctggcacagc acccggcgag catgacgcac tccagttaca cgccacaaga
     4621 gcgggcgcat cacgggatat cagaggggct ggtgaggttg tcagtggggc tggaggatgt
     4681 ggaggacctg ctggcagata tcgagttggc gttggaggcg tgtgcaggat ccaaaggtgg
     4741 acatcatcat catcatcata agctaatagc gagcgctgac
//
