LOCUS       pQE-81L-Kan.        4543 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial lacIq vector with a kanamycin resistance marker for 
            expressing N-terminally 6xHis-tagged proteins. For other reading 
            frames, use pQE-80L-Kan or pQE-82L-Kan.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-81L-Kan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Because this vector contains the lacIq gene, the pREP4 plasmid is 
            not needed.
FEATURES             Location/Qualifiers
     source          1..4543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             101..106
                     /note="ribosome binding site"
     CDS             115..117
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             127..144
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    147..194
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    194..204
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     terminator      210..304
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             348..1004
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1072..1158
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    complement(1217..1238)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1254..2333)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(2334..2411)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    2603..2743
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2929..3517)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3627..4439)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
      121 ggatctcacc atcaccatca ccatacggat ccgcatgcga gctcggtacc ccgggtcgac
      181 ctgcagccaa gcttaattag ctgagcttgg actcctgttg atagatccag taatgacctc
      241 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
      301 gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
      361 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
      421 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
      481 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
      541 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
      601 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
      661 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
      721 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
      781 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
      841 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
      901 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
      961 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
     1021 gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa
     1081 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1141 ctcctgagta ggacaaatcc gccctctaga ttacgtgcag tcgatgataa gctgtcaaac
     1201 atgagaattg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg
     1261 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1321 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
     1381 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt
     1441 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg
     1501 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg
     1561 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga
     1621 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg
     1681 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc
     1741 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
     1801 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata
     1861 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
     1921 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
     1981 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
     2041 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
     2101 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
     2161 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
     2221 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
     2281 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
     2341 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcaccat
     2401 tcgatggtgt cggaatttcg ggcagcgttg ggtcctggcc acgggtgcgc atgatctaga
     2461 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     2521 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     2581 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     2641 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     2701 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     2761 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2821 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2881 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2941 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3001 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3061 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3121 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3181 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3241 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3301 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3361 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3421 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3481 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3541 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tggcgttaag
     3601 ggattttggt catgaattaa ttcttagaaa aactcatcga gcatcaaatg aaactgcaat
     3661 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     3721 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     3781 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     3841 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct
     3901 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
     3961 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa
     4021 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     4081 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc
     4141 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     4201 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
     4261 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     4321 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     4381 tccatgttgg aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata
     4441 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga ccatgacatt
     4501 aacctataaa aataggcgta tcacgaggcc ctttcgtctt cac
//
