LOCUS       Exported                3430 bp DNA     circular SYN 11-SEP-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   11111111
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(1100..3430,1..1099)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(34..894)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(895..999)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1109..1153
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    1129..1145
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1161..1177
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1232..1249
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      1272..1366
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      2138..2224
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    2379..2519
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2705..3293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
       61 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      121 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
      181 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      241 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      301 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      361 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      421 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      481 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      541 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      601 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      661 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      721 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      781 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      841 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
      901 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
      961 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1021 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     1081 gaggcccttt cgtcttcacc tcgagaaatc ataaaaaatt tatttgcttt gtgagcggat
     1141 aacaattata atagattcaa ttgtgagcgg ataacaattt cacacagaat tcattaaaga
     1201 ggagaaatta accatgggag gatccagatc tcatcaccat caccatcact aagcttaatt
     1261 agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt
     1321 gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa gctagcttgg
     1381 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     1441 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     1501 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     1561 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     1621 cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     1681 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     1741 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     1801 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     1861 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     1921 tcaccatgca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     1981 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     2041 cagtactgcg atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct
     2101 taaacgcctg gggtaatgac tctctagctt gaggcatcaa ataaaacgaa aggctcagtc
     2161 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     2221 aaatccgccc tctagagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2281 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2341 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     2401 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     2461 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     2521 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2581 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2641 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2701 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2761 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2821 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2881 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2941 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3001 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3061 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3121 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     3181 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3241 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3301 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3361 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3421 ttaaatcaat
//
